• Genomics & Informatics
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• 제5권1호
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• pp.30-31
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• 2007

Many visualization tools have been designed to aid information processing during whole genome projects. We have developed a trace viewer program, ChroView, which can read a chromatogram file and display the chromatogram traces of the four bases. The program can be used to examine sequencing quality and base-calling errors. It can also help researchers to edit and save base-calling results while browsing the traces. Additionally, this program has a basecalling feature which can produce supplementary data for validation of the results from other base-calling programs.

• 대한본초학회지
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• 제23권1호
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• pp.109-116
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• 2008

Objectives : This study was performed to investigate the discriminative criteria of 6 kinds of Achyranthis Radix by HPLC/DAD. Methods : 20-hydroxyecdysone is isolated by silica gel column chromatography ($CHCl_3$:MeOH, 7:1-1:1 v/v) and identified by nuclear magnetic resonance, A high-performance liquid chromatographic method with diode array detection was used to identify 20-hydroxyecdysone in A. japonica. The analysis was performed using $C_{18}$ column with isocratic elution consisted of 18% acetonitrile and 82% water and the detection was carried out by DAD at 254 nm. 6 kinds of Achyranthis Radix from different locations were extracted in MeOH. Each extracts was analyzed by HPLC in same condition as used in analysis of 20-hydroxyecdysone. The identities of each extracts were determined by comparing the retention time and UV spectrum with that of reference compound. Results : 1. A. japonica and A. bidentata showed the similar patterns of HPLC chromatogram and 20-hydroxycedysone was present in both of them because the peaks having the same retention time and UV spectrum as 20-hydroxyecdysone were shown in the HPLC chromatograms of A. japonica and A. bidentata 2. Cyathula officinalis and C. capitata showed the similar patterns of HPLC chromatogram. The peak having the same retention time and UV spectrum as 20-hydroxyecdysone was shown in the HPLC chromatogram of C. capitata but not shown in the HPLC chromatogram of C. officinalis. 3. Two species of medicinal drugs from Sacheon province showed similar patterns of HPLC chromatogram. Achyranthis Radix from Sacheon(wild) did not have 20-hydroxycedysone but Achyranthis Radix from Sacheon(cultivated) showed the peak having the same retention time as 20-hydroxyecdysone but UV spectrum of the peak was different from that of 20-hydroxyecdysone. Conclusions : These results suggested that 20-hydroxyecdysone could be the discriminative criteria for Achyranthis Radix contain 20-hydroxyecdysone though they belong to different genus and species. And the patterns of HPLC chromatogram also could be the discriminative criteria as the different species of Achyranthis Radix belonging to the same genus showed similar patterns of HPLC chromatogram.

• 약학회지
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• 제4권1호
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• pp.8-11
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• 1959

The method for the estimation of trace elements in hair is studied on this paper modifying the circular paper chromatography method shown by Giri and Balakrishnan for the estimation of vitamins from the multivitamin preparation. The elements studied are Ni, Al, Mn, Mg, Zn, Ca, Co, Cu, and Fe. The content of the element is estimated quantitatively by comparison the size and the color density of spots of the standard chromatogram prepared by the known amount of each standard substance and the chromatogram prepared from sample solution, after checking those chromatogram qualitatively. The comparative study has been made between the results from this method and the results from the other methods which are applying routinely at this laboratories. The experimental errors at each elements were within 10% limits. There was no interference between each elements. It is recommendable that this method can be applied to treat numerous speciemens saving times within experimental error of 10%.

• 대한예방한의학회지
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• 제9권2호
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• pp.125-134
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• 2005

It is well known that Crataegus Fructus strengthens the Stomach and Spleen, helps digestion, tonify stomachache, and decreases bleeding. A major component isolated from this herb consist of carotene, riboflavin, quercetin, chlorogenic acid, amygdalin, ursolic acid etc. In this study, we aimed to analyze the changes of liquid chromatogram pattern, one of major standardization method, to determine the quality of Crataegus Fructus.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 1991년도 가을 학술발표회 논문집
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• pp.1-4
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• 1991

Relationship between againg and characteristic change of asphalt is not well known through asphalt rheolopy. Therefore, this study was conducted to evaluate characteristic change of asphalt cement due to againg based on experimental results of chromatogram and selected physical properties. Currently, three virgin asphalt cements were tested for physical properties and chromatograms before and after artificially conditioning for againg. Results in the current study showed that againg caused significant changes of physical properties and increase of larger size molecules and approximately corresponding reduction of smaller size molecule in asphalt cement molecular size distribution.

• 콘크리트학회지
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• 제3권1호
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• pp.71-78
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• 1991

3종류의 AC-20 아스팔트 시멘트의 분자입자분포를 고압-겔투수 크로마토그래프(HP-GPC)를 이용하여 관찰하였다. 건조상태의 습윤상태의 인장강도와 뤠질리언계수 측정을 위하여 한종류의 골재와 아스팔트 시멘크를 사용하여 실험실에서 역청콘크리트 공시체를 제조하였다. 이들의 역학적 성질시험결과와 아스팔트 시멘트 크로마토그램의 형태를 분석하여 상호간의 관계를 조사하였다. 희귀분석 결과는 역학적 성질과 크로마토그램의 분할면적비율간에 강한 상관관계가 있음을 보여주었다. 이 희귀분석 결과로부터 수학적 추정모델이 얻어졌다. 연구결과 아스팔트 크로마토그램의 특성이 정해진 아스팔트콘크리트의 역학적 성질추정이 잘 사용될 수 있음을 보여주었다.

• KSBB Journal
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• 제15권1호
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• pp.60-65
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• 2000

크로마토그래피의 거동을 수치 모사하기 위하여, retention time method와 moment method에 의해 계산된 파라미터들을 흡착 등온선 모델식에 사용하였다. 이 경우, 이론치와 비교하기 위해서 등용매 조건하게 유기산 이온 크로마토그래피 실험을 수행하였고, 크로마토그램의 거동을 설명하는 수학적 모델로 equilibrium-dispersive model을 사용하였다. Moment method에서는 평균체류시간을 나타내는 1차 모멘트, $m^2$1와 분배 계수 K의 관계로 나타낼 수 있으며, 실험을 통해 분배 계수는 쉽게 구해 질 수 있다. 운전 변수의 변화에 따른 크로마토그램의 특성을 살펴보기 위해서 유기산의 농도, 이동상의 유속, 용리액의 이온 강도 그리고 용질의 주입량을 변화시켜 수치모사를 수행하였다. 각각의 조건에 대해서 크로마토그램은 용질의 농도가 증가할수록, 유속이 증가할수록, 이온 강도가 증가할수록 평균 체류 시간이 감소하고 날카로운 피크를 얻을 수 있었으며, retention time method에서 보다 moment method와 data fitting에 의해 계산된 파라미터들이 실험치와 더 잘 일치함을 볼 수 있었다. 본 연구를 바탕으로, gradient 조건에서 유기산에 대한 이온 크로마토그래피의 거동 연구로 확장할 수 있다.

• Archives of Pharmacal Research
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• 제9권3호
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• pp.131-138
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• 1986

A specific and sensitive gas chromatographic (GC) procedure with the flame photometric detector (FPD) was developed for determination of barbiturates such as barbital, allobarbital, secobarbital, phenobarbital and thiopental in plasma. In order to evaluate the performance of the FPD, the results were campared with those of the flame ionization detector (FID). After extraction of barbiturates from plasma, the barbiturates were quantitatively N, N-dimethylthiometyl (MTM)-derivatized with methylthiomethyl chloride in 1, 8-diazabicyclo [5, 4, 0] undec-7-ene catalyst. The data indicate that the FPD is about 4 times more sensitive than the FID for barbiturates, although it is less reproducible. The FPD also produced chromatogram with less back ground for extracted plasma sample. The FPD also produced chromatogram with less background for extracted plasma sample. The minimum detectable amount of MTM-thiopental on 3% OV-225 column was 4, 4fmol and that of other MTM-barbiturate was about 10.0fmol.

• Journal of Ginseng Research
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• 제21권3호
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• pp.209-213
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• 1997

Sesquiterpenes of Korean ginseng (Panax ginseng C.A. Meyer, KG) and American ginseng (Panax quinquefolium, AG) were Isolated by simultaneous distillation and extraction (SDE) with 30% methanol and n-hexane or adsorption using C18 Sep-Pak . The sesquiterpenes in KG and AG were compared each other by using TLC and GC/MS. Thin layer chromatogram of KG gave 4~5 spots of terpenes colored by vanillin-sulfuric acid, while AG did one major spot. Total ion chromatogram of KG showed about 30 peaks of sesquiterpene having molecular weight 204. Among these, 9 components such as alloaromadendrene, germacrene B, isocaryophyllene, $\alpha$-neoclovene, ${\gamma}$-muurolene and $\beta$-panasinsene together occupied 81.5% of total sesquiterpenes identified. In AG, however, only 3 components, i.e., isocaryophyllene, $\beta$-bisabolene, $\beta$-sesquiphellandrene occupied 79.3% of total sesquiterpenes identified. Especially isocaryophyllene was a dominant component of AG occupying 56.6%. Eight sesquiterpenes, including alloaromadendrene, germacrene B and $\alpha$-humulene, were not detected in AG. These results indicate that sesquiterpenes could be used as indices for the chemical difference between KG and AG.

• Journal of Microbiology and Biotechnology
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• 제5권3호
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• pp.143-148
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• 1995

A strain producing soybean paste flavor was isolated from traditional Korean soybean paste. The isolate was identified as Bacillus subtilis PM3. The neutral fraction representing the traditional soybean paste aroma was obtained from the whole volatile components produced by B. subtilis PM3 in cooked soybean. Each separated peak from the neutral fraction of gas chromatogram was identified by gas chromatography-mass spectrometry (GC/MS) and Kovat's retention index, and the aromas of each peak were investigated by a sniffing test with the exercise panel. The twenty-nine components, including six character impact compounds and twelve components of flavors of Korean soybean paste, were confirmed. Some regions of gas chromatogram represented the soybean paste odor. It has been confirmed that traditional Korean soybean paste can be manufactured with the strain B. subtilis PM3.