• 대한수의학회지
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• 제6권1호
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• pp.1-9
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• 1966

돈(豚)콜레라는 세계각처(世界各處)에서 널리 유행(流行)되는 돼지의 중요(重要)한 질병(疾病)이다. 그러나 이 질병(疾病)을 혈청학적(血淸學的)으로 진단(診斷)할 수 있는 간단(簡單)하고 고도(高度)로 특이(特異)한 방법(方法)은 아직 없다. 이 연구(硏究)에서는 보체결합반응(補體結合反應)을 이용(利用)한 보다 우수한 혈청적진단법(血淸的診斷法)을 실험(實驗)하여 좋은 성적(成績)을 얻었다. 검출항원(檢出抗原)은 감염돈(感染豚)의 췌장유제(膵臟乳劑)이 양성예(陽性例)에서의 역가(力價)는 1/16 이상이였다. 항혈청(抗血淸)은 조직배양원(組織培養源) 바이러스 재료(材料)를 가토(家兎)에 접종(接種)하여 만들었고 이의 역가(力價)는 1/32 이상이었다. 보체(補體)는 1.1~1.2 충분단위(充分單位)를 사용하였고 감작적혈구(感作赤血球)는 2 단위(單位) 2%로 하였다. 이 방법(方法)으로써 감염돈(感染豚)의 95% 이상이 특이적(特異的)으로 검출(檢出)될 수 있었고, 비감염돈(非感染豚)은 97% 이상이 음성(陰性)으로 검출(檢出)될 수 있었다. 따라서 이 방법(方法)은 돈(豚)콜레라를 진단(診斷)하는데 이용(利用)될 수 있는 보다 낳은 혈청학적(血淸學的) 방법(方法)으로 믿어진다.

• 대한의생명과학회지
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• 제23권3호
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• pp.175-184
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• 2017

Cholera toxin (CT) is an ADP-ribosylating bacterial exotoxin that has been used as an adjuvant in animal studies of oral immunization. The mechanisms of mucosal immunogenicity and adjuvanticity of CT remain to be established. In this study, we investigated the role of indoleamine 2,3-dioxygenase (IDO), which participates in the induction of immune tolerance, in CT-mediated breakdown of oral tolerance. When IDO-deficient ($IDO^{-/-}$) mice and their littermates were given oral ovalbumin, significant changes in antibody responses, footpad swelling and $CD4^+$ T cell proliferation were not observed in $IDO^{-/-}$ mice. Feeding of CT decreased IDO expression in mesenteric lymph nodes (MLN) and Peyer's patch (PP). CT-induced downregulation of IDO expression was reversed by inhibitors of nuclear factor-kappa B (NF-${\kappa}B$), pyrrolidine dithiocarbamate and p50 small interfering RNA. IDO expression was downregulated by the NF-${\kappa}B$ inducers lipopolysaccharide and tumor necrosis factor-${\alpha}$. CT dampened IDO activity and mRNA expression in dendritic cells from MLN and PP. These data indicate that CT disrupts oral tolerance by activating NF-${\kappa}B$, which in turn downregulates IDO expression. This study betters the understanding of the molecular mechanism underlying CT-mediated abrogation of oral tolerance.

• Journal of Preventive Medicine and Public Health
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• 제3권1호
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• pp.31-36
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• 1970

An epidemiological survey on cholera was made toward the 291 cases admitted in Pusan National University Hospital. The results were as follows. 1. The most predominant age groups among all cholera patients were from 30 to 39(21.3%). 2. 74.9% of all cases got under the educational background of primary school and 69.4% of all cases occurred at the low income group. 3. Daily average numbers of patient were 6.2 and maximum numbers wore 15, August 19, 1970. 4. Among the food eaten prior to onset, drinking water was the most predominant with 16.2% and next, food and noodle groups were 14.8% and 10.3% respectively. 5. Regarding incubation period, it was found that 'within 48 hours' was 83.5%. 6. Patients occurred mainly in Suh Ku area with 105(36.1%) of all cases. 7. Carrier rates among family members were 1.78%. 8. Carrier rates among residents of epidemic area were 0.12%.

• 대한수의학회지
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• 제25권1호
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• pp.69-75
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• 1985

Hog cholera serum neutralizing antibody of piglets and sows were titrated by means of END SN method. The piglets of a variety of ages, precolostrally immunized with LOM living HC vaccine were subjected to the test. The sows were vaccinated with lapinized living HC vaccine after 25 days from the parturition. Throughout the studies the following results were obtained and summarised. 1. Hog cholera antibody titers of inbred sows immunized with lapinized living HC vaccine after 25 days from parturition were high except Hampshire group(Table 2). 2. Sows, different stage of the pregnancy or the day of parturition, and of 3 way crossed, that were immunized with lapinized living HC vaccine have shown no significant difference on HC antibody titer(Table 2, 3). 3. HC antibody titers of piglets, immunized with a single dose of LOM HC vaccine before feeding colostrum, were high in case of the younger group(1 week) compare to the older(7 week) (Table 4). 4. The piglets that were booster immunized with LOM HC vaccine at the age of 7 weeks have shown an inconsistent antibody titers(Table 5).

• 대한수의학회지
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• 제25권2호
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• pp.103-112
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• 1985

국내에서 분리된 enterovirus의 병원성을 관찰하기 위하여 1~2일령의 자돈 10두와 35일 령의 유돈 6두에 enterovirus 조직배양부유액을 뇌내 또는 근육내 주사한 후 임상 및 병리학적으로 관찰하였던 바 다음과 같은 결과를 얻었다. 임상적으로는 체온의 상승, 보양창랑, 보행실조, 유약성 마비 및 고도의 삭수가 관찰되었다. 병리조직학적으로는 중추신경계 전반에 걸쳐 수막하 세포침윤, 혈관주위 원형세포침윤, 신경세포의 변성, 미만성과 한국성 gliosis, glial nodule의 형성 등이 관찰되었으며 백질부보다 회백질부에서 다소 심한 경향이었고 배측 신경절염이 전 실험예에서 인정되었다. 한편 hog cholera백신과 enterovirus의 공동주사예에서는 중추신경계의 병변이 enterovirus 단독주사예에 비해 급격히 진행되는 경향이었다. 이상의 소견들을 종합해 볼때 국내에서 분리된 enterovirus는 뇌내 또는 근육내에 접종하였을 때 자돈에서 polioencephalomyelitis를 일으킬 수 있는 병원성이 강한 형으로 추측되며 hog cholera백신과 enterovirus를 공동주사한 예에서 병변의 진행이 급격한 점으로 보아 enterovirus의 자연감염에 hog cholera 백신 등의 stress요인이 작용될 것으로 추측된다.

• 대한간호
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• 제8권5호통권43호
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• pp.23-26
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• 1969

• 대한미생물학회지
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• 제14권1호
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• pp.39-47
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• 1979

• 대한수의사회지
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• 제27권12호
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• pp.725-728
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• 1991

The END method for titration of hog cholera virus and its serum neutralizing antibody was improved using ST cells grown and kept in modified media. ST cells were grown in Eagles media containing 0.5$\%$ lactalbumin hydrolysate, 10$\%$

• 환경위생공학
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• 제2권2호
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• pp.61-67
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• 1987

The genus Vibrio contains some of the most important intestinal pathogens of humans, including Vibrio cholerae, the cause of epidemic Asiatic cholera. A group of organisms which have been reffered to as the non-agglutinating vibrio (NAG) do not agglutinate in the Vibrio cholerae 0 group 1 antisera, but are indistinguishable from the 0-1 group both chemically and genetically. Non-O-l Vibrio cholerae can cause isolated as well as focal outbreaks of diarrhea, but the volume of fluid loss does not approach that of classic cholera, and the disease is usually self-limiting. These free-living organisms are found world-widely distributed in the environment including sewage, contaminated water, estuaries, seafood and animals. These strains involved in several cases were isolated from the environment and some patients of diarrhea, and a few epidemiologic reports indicated the wide distribution of the strains throughout the country, giving an attention to the role the organisms may play in an outbreak of diarrhea in Korea. More research on the epidemiology, serologic typing and virulence of the group of organisms, should be, therefore, done to obtain a complete understanding of their role in human disease.

• 대한수의사회지
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• 제33권9호
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• pp.544-553
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• 1997

Outbreaks of hog cholera(HC; classical swine fever) in 10 farrow-to-finish farms of regional corporative swine unit in the vicinity of Taegu(southeastern part of Korea) were investigated during the period of 6months from January to June 1996. The disease