• 한국발생생물학회지:발생과생식
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• 제15권4호
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• pp.309-313
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• 2011

SOHLH2 is a novel germ cell-specific transcription factor that is crucial for folliculogenesis in the ovary and spermatogenesis in the testis. SOHLH2 represents a candidate gene for infertility with premature ovarian failure. We analyzed whether mutations in the SOHLH2 gene in 98 Korean women with premature ovarian failure. The sequence analysis identified six novel SNPs (c.431-41G>C, c.656A>T, c.1000+27C>T, c.1000+33G>T, c1258-106G>A, and c.2094+ 11T>C) from Korean patients with premature ovarian failure. The c.656A>T found in exon 7 results in change of an amino acid, tyrosine to phenylalanine. Functional mutations in SOHLH2 gene are rare in Korean women with premature ovarian failure.

• Clinical and Experimental Reproductive Medicine
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• 제35권4호
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• pp.239-245
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• 2008

• Investigative Magnetic Resonance Imaging
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• 제20권4호
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• pp.254-258
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• 2016

Spontaneous perforation of the bile duct without any traumatic or iatrogenic injury is extremely rare. We report a case of abscess formation related to spontaneous perforation of the common bile duct by a gallstone, mimicked a cholecochal cyst.

• Journal of Acupuncture Research
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• 제22권4호
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• pp.13-19
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• 2005

Objectives : This report intended to estimate effect taken by using acupuncture, herbal medication on the patient with Pyogenic Spondylitis Methods : From 17 December, 2003 to 30 August, 2004. The patient received TDP, Infra-Red and Acupuncture therapy. Results : After being hospitalized for 8 months, the patient's Motor Grade, VAS, ODI, Nurick's grading system were improved each from III to IV+, from 5 to 4, from 21.25 to 17.5, from Gr. IV to Gr. II. Conclusion : We considered that oriental treatment has an useful effect on Pyogenic spondylitis patient's treatment and recovery.

• Clinical and Experimental Reproductive Medicine
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• 제48권4호
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• pp.283-294
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• 2021

A genetic etiology of male infertility is identified in fewer than 25% of infertile men, while 30% of infertile men lack a clear etiology, resulting in a diagnosis of idiopathic male infertility. Advances in reproductive genetics have provided insights into the mechanisms of male infertility, and a characterization of the genetic basis of male infertility may have broad implications for understanding the causes of infertility and determining the prognosis, optimal treatment, and management of couples. In a substantial proportion of patients with azoospermia, known genetic factors contribute to male infertility. Additionally, the number of identified genetic anomalies in other etiologies of male infertility is growing through advances in whole-genome amplification and next-generation sequencing. In this review, we present an up-to-date overview of the indications for appropriate genetic tests, summarize the characteristics of chromosomal and genetic diseases, and discuss the treatment of couples with genetic infertility by microdissection-testicular sperm extraction, personalized hormone therapy, and in vitro fertilization with pre-implantation genetic testing.

• 한국발생생물학회지:발생과생식
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• 제12권2호
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• pp.141-149
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• 2008

This study was conducted to develop an efficient cryopreservation method of human embryonic stem (ES) cells using vitrification. In an initial experiment, sub-clumps of human ES cells (CHA-hES3 and CHA-hES4) were vitrified using grids after incubation with STO feeder cells for 1 or 16 h (Groups 1-1 and 1-2, respectively). After storage for $2{\sim}4$ months, thawed clumps were re-plated on a fresh feeder layer. The survival rates of warmed CHA-hES3 and CHA-hES4 cells of Group 1-2 were significantly higher than those of the corresponding Group 1-1 cells. In the second experiment, human ES cells were vitrified after incubation with feeder or feeder-conditioned medium (Groups 2-1 to -7). Relative mRNA expression of BM proteins and survival rates were increased following incubation of ES cells with fresh feeder cells for 16 h. In conclusion, increasing of tight adhesion between ES cells by extended incubation with feeder could reduce cryoinjury after vitrifying/warming.

• Reproductive and Developmental Biology
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• 제31권4호
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• pp.267-272
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• 2007

Human embryonic stem (ES) cells are derived from the inner cell mass of the preimplantation embryo and have the capacity to differentiate into various types of cells in the body. Hence, these cells may potentially be an indefinite source of cells for cell therapy in various degenerative diseases including neuronal disorders. For clinical applications of human ES cells, directed differentiation of these cells would be necessary. The objective of this study is to develop the culture condition for the expansion of neural precursor cells derived from human ES cells. Human ES cells were able to differentiate into neural precursor cells upon a stepwise culture condition. Neural precursor cells were propagated up to 5000-fold in cell numbers over 12-week period of culture and evaluated for their characteristics. Expressions of sox1 and pax6 transcripts were dramatically up-regulated along the differentiation stages by RT-PCR analysis. In contrast, expressions of oct4 and nanog transcripts were completely disappeared in neural precursor cells. Expressions of nestin, pax6 and sox1 were also confirmed in neural precursor cells by immunocytochemical analysis. Upon differentiation, the expanded neural precursor cells differentiated into neurons, astrocytes, and oligodendrocytes. In immunocytochemical analysis, expressions of type III ${\beta}$-tubulin and MAP2ab were observed Presence of astrocytes and oligodendrocytes were also confirmed by expressions of GFAP and O4, respectively. Results of this study demonstrate the feasibility of long-term expansion of human ES cell-derived neural precursor cells in vitro, which can be a potential source of the cells for the treatment of neurodegenerative disorders.

• 건축역사연구
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• 제14권2호
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• pp.143-157
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• 2005

This study aims to analyze functions and characteristics of 'Cha-il(遮日)' which was used at ritual events(宮中儀禮行事) in the Joseon dynasty, Joseon dynasty had many ritual events related with O-Rae(五禮). Cha-il was almost used at important ritual events, especially at the congratulatory events. The congratulatory events(宮中宴享) are the representative events were held with many temporary install facilities(假設施設) in a palace. Cha-il was a kind of Korean traditional membrane structures. Cha-il was set up for cutting off interior space from sunlight and rain. In general, Cha-il was Consisted of Cha-il-jang(遮日帳:woven fabric), Cha-il-jook(遮日竹:bamboo column), some ropes, and fixing wares. In the congratulatory events, three types of Cha-il. were set up. Baek-mok-Dae-Cha-il(白木大遮日)was made of cotton cloth. Yu-Dun-Cha-Il(油芚遮日) was made of oiled paper or oiled cotton cloth. Man-Joen-Cha-Il(滿箭遮日) consisted of wooden structure. Baek-mok-Dae-Cha-il(白木大遮日)was set up on the most important area of stage for protecting from sunlight. Yu-Dun-Cha-Il was set up on less important area of stage for protecting from sunlight and rain. Man-Joen-Cha-Il was set up below Baek-mok-Dae-Cha-il for supporting and draining raindrops off. The results of this study were as follows; Functions of Cha-il were to protect ritually space from sunlight and rain, and to extend ritually space, and to reconstruct ritually space. Cha-il was the peculiar temporary install facility which differed from other countries.

• Journal of Acupuncture Research
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• 제21권4호
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• pp.263-268
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• 2004

Objective: The purpose of this case study is to show a case of ulcer due to buerger's disease improved by conservative acupuncture therapy. Methods : The patient was treated from 29 December, 2003 to 12 April, 2004. The patient received TDP, Infra-Red and Acupuncture therapy. Results: We evaluated through visual analog scale(VAS) and picture of patient's hand. Conclusion: We suggest to treat ulcer resulting from buerger's disease with Acupuncture therapy as a conservative therapy and would like to research acupuncture treatment plan for the further treatment.

• 생물정신의학
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• 제21권4호
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• pp.161-167
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• 2014

Objectives This study aims to investigate possible associations between proneness toward smartphone addiction and certain psychopathological variables to evaluate the psychopathological meaning of smartphone addiction. Methods Questionnaires were assigned to 755 adults between September and November 2012. We used the Korean Smartphone Addiction Proneness Scale (SAPS), the Beck Depression Inventory (BDI), the Beck Anxiety Inventory (BAI), the Obsessive-Compulsive Inventory-Revised (OCI-R), and the Barratt Impulsivity Scale-11 (BIS-11). Subjects were classified into two groups according to the scores of the SAPS ; the addiction proneness group and the normal-user group. Results The addiction proneness group had significantly higher scores than the normal-user group in the BDI, BAI, OCI-R, and BIS-11. The scores of the SAPS were positively correlated with the scores of the BDI, BAI, OCI-R, and BIS-11. Using logistic regression analysis, smartphone addiction proneness showed a significant association with BIS-11. Conclusions The results of this study suggest that smartphone addiction proneness may be associated with depression, anxiety, obsessive-compulsive symptoms, and impulsivity. Furthermore, impulsivity could be a vulnerability marker for smartphone addiction proneness.