• Title/Summary/Keyword: Choi Chang Hak

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Effects of Rubus coreanus byproducts on intestinal microbiota and the immune modulation

  • Yu, Da Yoon;Kim, Sung Hak;Kim, Jeong A;Kim, In Sung;Moon, Yang Soo;Lee, Sang Suk;Park, Hwa Chun;Jung, Jong Hyun;Chung, Yi Hyung;Shin, Dae Keun;Nam, Ki Chang;Choi, In Soon;Cho, Kwang Keun
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.3
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    • pp.429-438
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    • 2018
  • Objective: Although the efficacy of Rubus coreanus (RC) byproducts as a feed additive has been recognized, its effects on intestinal microorganisms and the immune system are still unknown. Methods: Six-week-old male rats were treated with 0.5% RC (T1), 1.0% RC (T2), and 1.5% RC (T3) for 4 weeks. Results: We found that treatment with RC byproducts significantly increased the daily gain of body weight and feed intake. Treg-cell differentiation was enhanced in the mesenteric lymph nodes and spleen from the rats fed with RC byproducts. Illumina sequencing showed that bacteria in the phylum Firmicutes decreased and while those in the phylum Bacteroidetes increased in RC-treated groups. Particularly, the pathogenic microorganisms in the family Peptococcaceae decreased, and the non-pathogenic families Lachnospiraceae and S24-7 increased. Quantitative polymerase chain reaction analysis showed that the RC byproducts increased the lactic acid bacteria Bifidobacterium spp., Oscillospira spp., Leuconostoc citreum, and Weissella cibaria in a concentration-dependent manner. Conclusion: RC byproducts may be effective in immunomodulation by affecting intestinal microorganisms.

Hematology and Serum Chemistry Values of Growing Miniature Pigs (성장 미니돼지의 혈액 및 혈청화학 성분의 연구)

  • Jung, Joo-Hyun;Chang, Jin-Hwa;Kim, Hak-Sang;Yoon, Jung-Hee;Choi, Min-Cheol
    • Journal of Life Science
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    • v.18 no.8
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    • pp.1169-1172
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    • 2008
  • The purpose of this study was to establish blood reference values for miniature pigs from various growth ages before pre-puberty. 12 miniature pigs from two swine farms were sampled by jugular venipuncture. Routine hematological and biochemical determinations were performed using whole blood and serum. For the variables examined in each age group the means and the standard deviations were calculated. In hematological values, there are significant changes (p<0.05) in RBC, hematocrit and platelet values in each group. In RBC and hematocrit values, 4-week group shows the lowest level, whereas in platelet values, 4-week group shows the highest level. In biochemical values, there are significant chnges (p<0.05) in glucose and GGT in each group, and in other biochemical values there are no significant changes during growing periods. The values presented here may be useful for knowing the normal values for miniature pigs and also screening the normal pigs in the early days of their life and during the developemental stages.

Activated Leukocyte Cell Adhesion Molecule: Expression in the Uterine Endometrium during the Estrous Cycle and Pregnancy in Pigs

  • Kim, Min-Goo;Shim, Jang-Soo;Seo, Hee-Won;Choi, Yo-Han;Lee, Chang-Kyu;Ka, Hak-Hyun
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.7
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    • pp.919-928
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    • 2011
  • The pig exhibits true epitheliochorial placentation, where the fetal membrane maintains attachment throughout pregnancy but does not invade into the maternal uterine endometrium. Accordingly, the expression and function of cell adhesion molecules are very important for embryo implantation and the establishment of pregnancy. In our recent microarray analysis, we found that activated leukocyte cell adhesion molecule (ALCAM) was expressed in the uterine endometrium during pregnancy in pigs. To better understand the roles of ALCAM in the establishment and maintenance of pregnancy, we examined ALCAM expression in the uterine endometrium during the estrous cycle and pregnancy in pigs. Real-time RT-PCR analysis showed that ALCAM was differentially expressed in the uterine endometrium during the estrous cycle and pregnancy, with the highest levels on D12 of pregnancy. ALCAM mRNA was localized to the luminal and glandular epithelial cells and to the trophectoderm of conceptuses during early pregnancy. The steroid hormones estrogen and progesterone had no effect on ALCAM expression in an endometrial explant culture study. Further, we found that ALCAM expression in the uterine endometrium from gilts with somatic cell nuclear transfer-derived embryos was not different from that in gilts with embryos from natural mating. ALCAM was expressed in a pregnancy stage- and cell type-specific manner in the uterine endometrium and conceptuses during pregnancy. These findings suggest that ALCAM may play a role in the establishment of pregnancy. Further analysis of ALCAM will provide insight into the implantation process and establishment of pregnancy in pigs.

A New Cultivar of Gypsophila paniculata with Powdery Mildew Resistance and Double Florets of Pink Color, 'Dream Pink' (흰가루병에 강한 분홍색 절화용 안개꽃 '드림핑크' 육성)

  • Cheong, Dong-Chun;Lim, Hoi-Chun;Choi, Chang-Hak;Song, Young-Ju;Kim, Jeong-Man
    • Horticultural Science & Technology
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    • v.29 no.5
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    • pp.500-502
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    • 2011
  • 'Dream Pink', a cultivar of Gypsophila paniculata was newly developed for cut flowers by Jeollabuk-do Agricultural Research & Extension Services. It has morphological characteristics of the intermediate plant type, double florets with many petal and two pistils, emarginate petal tip and campanulate calyx shape. Also 'Dream Pink' had several other traits such as strong flower stalk, deep pink flower color, resistance to powdery mildew and pest injury. Furthermore, 'Dream Pink' produced higher number of nodes, primary branches, longer flower stalk length with long intermodal length. In subalpine area, 'Dream Pink' cultivar showed narrow branching angle as compare to 'My Pink', during the summer cultivation. Blooming in 'Dream Pink' was delayed by 5 days, its florets were broader with more number of petals as compare to 'My Pink'.

The Superficial Inferior Epigastric Artery Flap and its Relevant Vascular Anatomy in Korean Women

  • Kim, Byung Jun;Choi, Jun Ho;Kim, Tae Hoon;Jin, Ung Sik;Minn, Kyung Won;Chang, Hak
    • Archives of Plastic Surgery
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    • v.41 no.6
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    • pp.702-708
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    • 2014
  • Background Lower abdominal soft tissue transfer is the standard procedure for breast reconstruction. However, abdominal wall weakness and herniation commonly occur postoperatively at the donor site. To reduce the morbidities of the donor site, the superficial inferior epigastric artery (SIEA) flap was introduced, but inconsistent anatomy of the SIEA has reduced its utility. In the present study, the anatomy of the superficial inferior epigastric vessels in Korean women was determined with regards to breast reconstructive surgery. Methods The vascular anatomies of the SIEA and superficial inferior epigastric vein (SIEV) were evaluated on 32 breast cancer patients receiving free transverse rectus abdominis musculocutaneous flap reconstruction after mastectomy. The existence, pulsation, location, external diameter, and depth of the SIEA and SIEV were measured at the lower abdominal incision level. Results SIEA and SIEV were present in 48/64 (75.00%) and 63/64 (98.44%) hemi-abdomens, respectively. Pulsation of the SIEA was found in 44/48 (91.67%) cases. The mean locations of SIEA and SIEV were +5.79 (${\pm}12.87$) mm, and -8.14 (${\pm}15.24$) mm from the midpoint between the anterior superior iliac spine and symphysis pubis, respectively. The mean external diameters of SIEA and SIEV were 1.20 (${\pm}0.39$) mm and 1.37 (${\pm}0.33$) mm, and they were found at a mean depth of 9.75 (${\pm}2.67$) mm and 8.33 (${\pm}2.65$) mm, respectively. Conclusions The SIEA was absent in 25% of Korean women and had a relatively small caliber. Therefore, careful preoperative assessment of the lower abdominal vasculature is required to achieve successful breast reconstruction using SIEA flaps.

Diagnostic Imaging of Nasal Adenocarcinoma in Four Dogs (개에서 비강 샘암종의 영상 진단 증례)

  • Jung, Joo-Hyun;Chang, Jin-Hwa;Oh, Sung-Kyoung;Kim, Kyoung-Min;Lee, Sung-Ok;Lee, Jung-Min;Kim, Hak-Sang;Yoon, Jung-Hee;Choi, Min-Cheol
    • Journal of Veterinary Clinics
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    • v.23 no.3
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    • pp.355-360
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    • 2006
  • Four dogs with similar respiratory signs were referred to Veterinary Medical Teaching Hospital, Seoul National University. The clinical signs observed in these cases were anorexia, nasal discharge, sneezing, epistaxis, ocular discharge, and exophthalmoses. The routine laboratory tests revealed leukocytosis in two cases. On the skull radiographs, soft tissue density filled nasal cavity with loss of turbinate detail and increased density in frontal sinuses were found in all cases. Lysis of nasal bone was seen in two cases. Lysis of zygomatic arch was seen in one case. On computed tomography scan images, asymmetrical destruction of turbinate and nasal septum, and the superimposition of a soft tissue mass over the turbinate with peripheral contrast enhanced effect were identified in all cases. Destruction of ipsilateral orbital bone and invasion to retrobulbar region were visualized in all cases. In addition, all cases had lysis of cribriform plate. Lysis of nasal bone and destruction of hard palate were seen in two cases. Swelling of submandibular lymph node and salivary gland was seen in a case. Invasion to brain was identified in a case. All cases were diagnosed as nasal adenocarcinoma by cytology with fine needle aspiration and curettage.

Preparation of $\beta$-Cyclodextrinized Cellulosic Fiber and Deodouring Property ($\beta$-시클로덱스트린화 셀룰로오스 섬유의 제조 및 소취성)

  • Choi, Chang-Nam;Hwang, Tae-Yeon;Ko, Bong-Kook;Kim, Ryong;Hong, Sung-Hak;Kim, Sang-Yool
    • Polymer(Korea)
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    • v.25 no.5
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    • pp.635-641
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    • 2001
  • $\beta$-Cyclodextrine/benzoic acid complex was prepared and reacted with cyanuric chloride (2,4,6-trichloro-1,3,5-triazine). Identification of complex formation and reaction was checked by FT-IR, UV-Vis, and EDX. By reacting this material with cotton fiber, the deodourant fiber was prepared. The deodourizing property was evaluated by the concentration changes of aqueous ammonia solution after flowing ammonia gas through the column titled with deodourant fiber prepared. The deodourizing property was increased with an increase of concentration of $\beta$-cyclodextrine unit in the fiber. In the case of $\beta$-cyclodextrine/benzoic acid complex, the deodourzing property was much increased, comparing with the $\beta$-cyclodextrine only. It was considered to be the binding of aamonia gas caused by benzoic acid in the complex.

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A Study on the Immobilization of Biomolecules on Poly(acrylic acid)-grafted MWCNTs Prepared by Radiation-Induced Graft Polymerization (방사선 그래프트 중합에 의하여 제조된 폴리(아크릴 산)이 그래프트된 탄소나노튜브에 생체분자 고정화에 관한 연구)

  • Jung, Chan-Hee;Lee, Byoung-Min;Hwang, In-Tae;Choi, Jae-Hak;Nho, Young-Chang;Hong, Sung-Kwon
    • Polymer(Korea)
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    • v.34 no.2
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    • pp.150-153
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    • 2010
  • In this research, biomolecule-immobilized multi-walled carbon nanotubes (MWCNTs) were prepared by using radiation-induced graft polymerization. For the immobilization of biomolecules, the surface of MWNCTs was functionalized by radiation-induced graft polymerization of acrylic acid. Based on the results of TGA and Raman spectroscopy it was found that acrylic acid was effectively graft-polymerized on the MWCNTs. Biomolecules such as DNA and proteins were immobilized onto the resultant poly(acrylic acid)-grafted MWCNTs. The results of the X-ray photoelectron spectroscopy and fluorescence microscopy confirmed that the biomoelcules were successfully immobilized on the poly(acrylic acid)-grafted MWCNTs.

A Case of Dermoid Cyst in Temporal Fossa (측두와에 발생한 피부모양기형낭종)

  • Lee, Hak Sung;Choi, Matthew Seung Suk;Ahn, Hee Chang;Lee, Jang Hyun
    • Archives of Craniofacial Surgery
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    • v.11 no.1
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    • pp.65-68
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    • 2010
  • Purpose: Dermoid cysts are benign neoplasms that are derived from both ectoderm and mesoderm. Approximately 7 percent of all dermoid cysts occur in the head and neck, as most common sites are the lateral ends of the eyebrows, the midline in the nasal root and neck. Rarely they can be found in the frontal sinus, temporal bone, maxilla and the floor of the mouth. Dermoid cysts in the temporal fossa are extremely rare. We experienced a characteristic dermoid cyst that occupied the temporal fossa. Methods: A 16-year-old man had a progressive enlarging mass on the left eyebrow. Computerized tomographic scan showed a bulging mass in the temporal fossa, and it had the density similar to that of fat. The size of the mass was $3{\times}3{\times}2cm$, and it was composed of high density of fat with clear margin. There was no bony invasion, but the mass was fixed on bone. Results: We performed the surgery through coronal incision under general anesthesia. Because the mass was closely connected with temporal fat pads, we removed this mass with some portion of temporal fat pads, avoiding damage to the facial nerve. The postoperative course was ordinary without complication. Conclusion: The reports about dermoid cyst on the temporal fossa is uncommon. However, if there is a mass in the temporal fossa which has the density similar to that of fat in CT scan, we should consider the possibility of dermoid cyst. We suggest that excision through coronal incision with bewaring temporal fat pad can induce good result.

Liquid Crystalline Properties of Schiff Base Mono- and Dimesogenic Compounds (Schiff Base 단일- 및 이메소제닉화합물의 액정성)

  • Park, Joo-Hoon;Choi, Ok-Byung;Lee, Jin-Seok;Kang, Keun-Myoung;Shin, Joo-Cheol;Kim, Ki-Hwan;Kim, Hak-Jin;Lee, Chang-Joon;So, Bong-Keun;Lee, Soo-Min
    • Korean Chemical Engineering Research
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    • v.43 no.1
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    • pp.176-180
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    • 2005
  • The liquid crystalline properties of a series of main chain liquid crystalline polymers and four series compounds consisting of aromatic type Schiff base mesogenic units and polymethylene flexible spacers were studied. The thermal and liquid crystalline properties were investigated by differential scanning calorimetry and on the hot stage of a polarizing microscope. The nature of the liquid crystalline phase of the polymers and compounds depended greatly on the length of the central polymethylene spacer and on the terminal alkoxy groups. Polymers I and Series III exhibited an even-odd effect in melting and isotropization temperatures but Series II and Series IV exhibited an even-odd effect in isotropization temperatures. They formed nematic and smectic mesophases in melts as judged by their optical textures observed through a polarizing microscope.