• Title/Summary/Keyword: Chlorophyll Content

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Enterococcus faecium LKE12 Cell-Free Extract Accelerates Host Plant Growth via Gibberellin and Indole-3-Acetic Acid Secretion

  • Lee, Ko-Eun;Radhakrishnan, Ramalingam;Kang, Sang-Mo;You, Young-Hyun;Joo, Gil-Jae;Lee, In-Jung;Ko, Jae-Hwan;Kim, Jin-Ho
    • Journal of Microbiology and Biotechnology
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    • v.25 no.9
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    • pp.1467-1475
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    • 2015
  • The use of microbial extracts containing plant hormones is a promising technique to improve crop growth. Little is known about the effect of bacterial cell-free extracts on plant growth promotion. This study, based on phytohormonal analyses, aimed at exploring the potential mechanisms by which Enterococcus faecium LKE12 enhances plant growth in oriental melon. A bacterial strain, LKE12, was isolated from soil, and further identified as E. faecium by 16S rDNA sequencing and phylogenetic analysis. The plant growth-promoting ability of an LKE12 bacterial culture was tested in a gibberellin (GA)-deficient rice dwarf mutant (waito-C) and a normal GA biosynthesis rice cultivar (Hwayongbyeo). E. faecium LKE12 significantly improved the length and biomass of rice shoots in both normal and dwarf cultivars through the secretion of an array of gibberellins (GA1, GA3, GA7, GA8, GA9, GA12, GA19, GA20, GA24, and GA53), as well as indole-3-acetic acid (IAA). To the best of our knowledge, this is the first study indicating that E. faecium can produce GAs. Increases in shoot and root lengths, plant fresh weight, and chlorophyll content promoted by E. faecium LKE12 and its cell-free extract inoculated in oriental melon plants revealed a favorable interaction of E. faecium LKE12 with plants. Higher plant growth rates and nutrient contents of magnesium, calcium, sodium, iron, manganese, silicon, zinc, and nitrogen were found in cell-free extract-treated plants than in control plants. The results of the current study suggest that E. faecium LKE12 promotes plant growth by producing GAs and IAA; interestingly, the exogenous application of its cell-free culture extract can be a potential strategy to accelerate plant growth.

Effect of Temperture and Light on Growth of Leaf Lettuce(Lactuca sativa L. var. crispa cv. Cheongchima) in Hydroponics (배양액 온도 및 차광정도가 청치마 상추의 생육에 미치는 영향)

  • 남상용;권용웅
    • Journal of Bio-Environment Control
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    • v.6 no.4
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    • pp.270-276
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    • 1997
  • This experiment was conducted to develop a practical method to growth in hydroponics conditions. For this purpose, leaf lettuce (Lactuca sativa L., cv. 'Cheongchima') was cultivated under different temperature and light conditions in experimental fields of Sahmyook university from March, 1991 to May, 1996. The visual quality score(VQS), a quantitative parameter to evaluate harvested leaf lettuce quality. The leaf lettuce cultivated at $25^{\circ}C$ solution temperature showed the highest fresh weight pe, plant (59.7g/plant), but lowest visual quality score (5.2), while leaf lettuce cultivated at 15$^{\circ}C$ solution temperature showed lower fresh weight per plant (25.2g/plant) but highest visual quality score (7.2). In general, growth of leaf lettuce was retarded by low solution temperature, but quality was higher than those of other solution temperature. Different day/night temperature of nutrient solutions 20/2$0^{\circ}C$ and 25/2$0^{\circ}C$ was highest in yield, but leaf type was grow longer, and visual quality are inferior to low temperature. Yield was decreased as 10 and 25% at 60 and 80% shading treatment, respectively. Chlorophyll content was decreased by shading. but plant height and root length were increased of leaf lettuce growth with hydroponics.

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Effect of Nutrient Solution Strength on Growth of Phalaenopsis in an Ebb and Flow System (Ebb and Flow 시스템을 이용한 호접란 수경재배시 생장에 미치는 배양액 농도의 영향)

  • An, Dong-Choon;Park, Seon-Hye;Been, Chul-Gu;Jeong, Byoung-Ryong
    • Journal of Bio-Environment Control
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    • v.16 no.3
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    • pp.199-204
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    • 2007
  • This experiment was conducted to investigate the effect of nutrient solution strength on the growth of miniature multiflora P. amabilis and P. Taisuco Red Jewel at two growth stages during four months cultivation in an ebb and flow system. Early stage plants gained the biggest leaf length and width when nutrient solution strength was EC 0.5 or $1.0dS{\cdot}m^{-1}$ in P. amabilis, and EC $1.5dS{\cdot}m^{-1}$ in P. Taisuco Red Jewel. Root length and weight were the greatest when nutrient solution strength was EC $0.5dS{\cdot}m^{-1}$ in both cultivars and this trend was also found in middle stage plants. Fresh and dry weights of leaves increased as nutrient solution strength was elevated and were the greatest when nutrient solution strength was EC $1.5dS{\cdot}m^{-1}$ in P. amabilis. On the contrary elevation of nutrient solution strength decreased fresh weight of roots, being the greatest when nutrient solution strength was EC $0.5dS{\cdot}m^{-1}$. This trend was also found in P. Taisuco Red Jewel and P. amabilis at middle growth stage. The T/R ratio and chlorophyll content increased with nutrient solution strength and were the greatest regardless of cultivar and growth stage when nutrient strength was EC $1.5dS{\cdot}m^{-1}$.

Comparative Study on the Leaf Pigment Compositions of Korean Ginseng(Panax ginseng C.A. Meyer) as Shade Plant (음지식물(陰地植物) 인삼(人蔘)(Panax ginseng C.A. Meyer)의 엽색소(葉色素) 구성(構成)에 대한 비교연구(比較硏究))

  • Lim, Sun-Uk;Lee, Mi-Kyong
    • Applied Biological Chemistry
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    • v.29 no.2
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    • pp.219-226
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    • 1986
  • The differences of pigment compositions in leaf chloroplast between sun and shade plant were analyzed to investigate the photo-induced destructive effects on shade species under the strong light. Ginseng was selected as a C-3 shade plant, soybean as C-3 sun species and corn as C-4 malate forming sun species. To study the effects of light, ginseng was divided into two subgroups; the 1 and 2 lines received sunlight a little more than those of 3 and 4 lines. Total amounts of chlorophylls, carotenes and xanthophylls were not considerably different among 3 and 4-lined ginseng, soybean and corn. However, the amounts of the three components of 1 and 2-lined ginseng and the chlorophylls content in corn leaves were smaller than those of others. The molar ratio of lutein to total carotenoids was significantly high in ginseng, that of violaxanthin was considerably high in corn and that of neoxanthin the highest in 1 and 2-lined ginseng among tested samples. Chlorophylls to carotenes ratio was 16.0 and highest in ginseng, 13.2 in soybean and 12.0 in corn. In 1 and 2-lined ginseng, the molar ratios of lutein and carotenes were lower while those of neoxanthin and violaxanthin were higher than those of the samples of more light. It was noticeable that an antheraxanthin-like epoxy carotenoid detected in soybean and corn leaves extracts was not observed in ginseng leaf extract.

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Relativity between Growth and Soil Environment of Viola orientalis Habitat (노랑제비꽃 자생지의 생육환경과 토양환경간 상관성)

  • You, Ju-Han;Jung, Sung-Gwan;Park, Kyung-Hun;Kim, Kyung-Tae;Lee, Woo-Sung
    • Current Research on Agriculture and Life Sciences
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    • v.25
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    • pp.1-5
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    • 2007
  • This study was carried out to offer the raw data of floricultural resource by scientific analyzing the growth and soil environment in Viola orientalis habitat, and establish the ecological characteristics in developing the relative model. In the results of soil factors analysis, there showed that acidity was pH 5.1, organic matter content of 9.1%, available $P_2O_5$ of 40.6mg/kg, exchangeable $K^+$ of $0.2cmol^+/kg$, exchangeable $Ca^{2+}$ of $3.5cmol^+/kg$, exchangeable $Mg^{2+}$ of $0.8cmol^+/kg$, cation exchange capacity(C.E.C) of $13.7cmol^+/kg$ and electrical conductivity(EC) of 0.4dS/m. The growth characteristics were surveyed that height was 12.4cm, leaf width of 2.5cm, leaf length of 3.0cm, flower width of 2.5cm, peduncle of 2.3cm and chlorophyll of $38.5{\mu}g{\cdot}mg^{-1}$.

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Breeding of Artificial Autotetraploids from Cold Hardness Lines of Yongchonppong and Yeongbyonppong (내동성계 재래뽕 용천뽕과 영변뽕의 동질4배체 육성)

  • 박광준
    • Journal of Sericultural and Entomological Science
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    • v.38 no.2
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    • pp.93-99
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    • 1996
  • By treatment(dropping) of 0.1~0.4% colchicine solution on the sprouts of winter buds of hard wood cutting slips for 4~5 days, two lines of artificial autotetraploids from Yongchonppong and one line from Yeongbyonppong were bred and the important cultivative characteristics of those new lines were as follows. The greentip sprouting stage of the new bred lines in spring season is later than the parental varieties by two days, but growth speed of the new lines after sprouting was faster than that of the parental varieties reaching the same level development with the parental varieties at the fifth leaf sprouting stage to be mid varieties same as the origins. The leaf shape of the new bred lines was wide round and the petioloes were long and thick. The thickness of leaf was thicker than the parental varieties by 17-33% and single leaf weight was heavy. The leaf area weight increased by 21-31% and the content of chlorophyll was also higher by 11-33%. With all the characteristics, the new breds produced good quality of leaves. The length and number of branches were shorter and less, respectively, than the parental varieties, but the internode length was either same or longer than the parents. Looking at the characteristics, the constitution of shoots was slightly inferior to the parental varieties. The cold hardness expressed by the death top rate of Sawonppong 23 and Sawonppong 24 was same level as that of Yongchonppong, but Sawonppong 25 was stronger than Yeongbyonppong in it with a high infection rate of dwarf disease. The productivity was lower than the parental varieties, but young shoot rate to shoot and branch and the ratio of leaf to young shoot were higher than the parental varieties. The fertility of Sawonppong 23 and Sawonppong 24 was comparatively high with 62% of cross success, but that of Sawonppong 25 was low with 23.9% of cross success.

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Drone-based Vegetation Index Analysis Considering Vegetation Vitality (식생 활력도를 고려한 드론 기반의 식생지수 분석)

  • CHO, Sang-Ho;LEE, Geun-Sang;HWANG, Jee-Wook
    • Journal of the Korean Association of Geographic Information Studies
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    • v.23 no.2
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    • pp.21-35
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    • 2020
  • Vegetation information is a very important factor used in various fields such as urban planning, landscaping, water resources, and the environment. Vegetation varies according to canopy density or chlorophyll content, but vegetation vitality is not considered when classifying vegetation areas in previous studies. In this study, in order to satisfy various applied studies, a study was conducted to set a threshold value of vegetation index considering vegetation vitality. First, an eBee fixed-wing drone was equipped with a multi-spectral camera to construct optical and near-infrared orthomosaic images. Then, GIS calculation was performed for each orthomosaic image to calculate the NDVI, GNDVI, SAVI, and MSAVI vegetation index. In addition, the vegetation position of the target site was investigated through VRS survey, and the accuracy of each vegetation index was evaluated using vegetation vitality. As a result, the scenario in which the vegetation vitality point was selected as the vegetation area was higher in the classification accuracy of the vegetation index than the scenario in which the vegetation vitality point was slightly insufficient. In addition, the Kappa coefficient for each vegetation index calculated by overlapping with each site survey point was used to select the best threshold value of vegetation index for classifying vegetation by scenario. Therefore, the evaluation of vegetation index accuracy considering the vegetation vitality suggested in this study is expected to provide useful information for decision-making support in various business fields such as city planning in the future.

Effects of Shading Treatment on Photosynthetic Activity of Acanthopanax senticosus (차광처리가 가시오갈피의 광합성 활성에 미치는 영향)

  • Kim, Pan-Gi;Lee, Kab-Yeon;Hur, Seong-Doo;Kim, Sun-Hee;Lee, Eun-Ju
    • The Korean Journal of Ecology
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    • v.26 no.6
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    • pp.321-326
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    • 2003
  • This study was conducted to investigate and effects of shading treatment on photosynthetic activity of Acanthopanax senticosus. We investigated plant growth, light response curve and A-Ci curve to photosynthesis of A. senticosus at 55%, 75%, and 90% shading treatment. As results, the ratio of above-ground/under-ground biomass was increased at 75% shaded condition and showed highest dry biomass. Under shaded conditions, plants had lower chlorophyll a+b content and a/b ratio and also showed thinner leave. But shaded plants showed higher leaf area and higher total leaf area per a plant. This apparently indicates adapted responses to shaded treatment. Effects of shading treatments on photosynthetic activity were higher in apparent quantum yield, carboxylation efficiency, re-phosphrylation but lower in light compensation point. These results suggested that higher photosynthesis rates in shaded treatments were due to activated carboxylation efficiency. Shading treatment had lower water use efficiency thatn controls but still higher than other tree species.

Influence of Drought and High Temperature on the Physiological Response and Yield in Hot Pepper (토양 건조 스트레스와 기온상승에 의한 고추의 생리반응 및 생산량 변화)

  • Lee, Sang Gyu;Lee, Hee Ju;Kim, Sung Kyeom;Mun, Bo heum;Lee, Jin Hyoung;Lee, Hee Su;Do, Kyung Ran
    • Journal of Environmental Science International
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    • v.27 no.4
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    • pp.251-259
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    • 2018
  • This study was conducted to determine the effects of combination of air temperature and soil water content on the growth, physiological disorder rate, and yield of hot peppers. The study was carried out in a typical plastic house (open on one side and with ventilation fans on the other side), which was maintained with gradient air temperature (maximum difference in air temperature: $6^{\circ}C$). The deficit irrigation (DI) treatment commenced 65 days after transplanting. The height of plant and fresh and dry weights of the stem increased at high air temperature (ambient + $6^{\circ}C$, extreme high temperature; EHT). Furthermore, the leaf area decreased significantly with the DI treatment. There were no significant differences in the stem diameter, number of branches, and fresh and dry weights of the leaves among all the treatments. The net photosynthesis rate of the full irrigation (FI) treatment was higher than that of the DI treatment. The photosynthesis rate at ambient air temperature was $19.7{\mu}mol\;CO_2m^{-2}{\cdot}s^{-1}$, the highest among all the treatments; however, the photosynthesis rate of the EHT treatment decreased by 60% ($12.3{\mu}mol\;CO_2m^{-2}{\cdot}s^{-1}$). Additionally, the formation of guard cells in the leaf was abnormal with the EHT treatment, and there was a decrease in translocation efficiency. The effects of air temperature treatment were more pronounced on the physiological disorder rate and yield. The physiological disorder rate of the EHT treatment was the highest under the DI treatment condition. The yield of the AFI (ambient air temperature with full irrigation) treatment was 3,771 kg/10a, the highest among all the treatments; however, the yield of the EHT treatment with DI and FI was 1,282 and 1,327 kg/10a, respectively. These results indicate that growth and physiological disorder rate improved with the EHT treatment; however, there was a decrease in yield. Furthermore, the formation of guard cells was abnormal and malfunctional.

Growth pf Plug Seedlings of Capsicum annuum and Lycopersicum esculentum as Affected by the Mixing Ratio of Aquafarm Waste Water Sludge in the Growing Medium (담수양어장 슬러지의 배지내 혼합비율이 고추(Capsicum annuum)와 토마토(Lycopersicum esculentum) 공정묘의 생장에 미치는 영향)

  • Lee, Eun-Ju;Hwang, Seung-Jae;Kim, Ik-Joon;Park, Young-Hoon;Jeong, Byoung-Ryong
    • Journal of Bio-Environment Control
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    • v.12 no.4
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    • pp.184-189
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    • 2003
  • This research was conducted to determine the effect of mixing ratio of aquafarm waste water sludge (AWWS) in the growing medium as a source of fertilizers on growth of plug seedlings of pepper (Capsicum annuum L.) and tomato (Lycopersicum esculentum Mill.). Increased mixing ratio of AWWS resulted in increased fresh and dry weights, leaf area, plant height, and total chlorophyll content, although there were slight differences in growth characteristics at 20 and 40 days after sowing. Concentration of AWWS affected insignificantly the percent dry matter, number of leaves, and length of the longest root. The addition of AWWS increased pH and decreased EC in the medium as compared to that of chemical fertilizer. Compared to the control of a liquid fertilizer, 4 or 8 kg AWWS${\cdot}45L^{-1}$ medium (Sludge 4) gave a similar or slightly better growth. Above results suggested that addition of about 4 kg AWWS${\cdot}45L^{-1}$medium is sufficient for seedling growth and the AWWS can be used as a substitute for the liquid fertilizer in plug seedling production.