• Journal of Microbiology and Biotechnology
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• v.5 no.4
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• pp.206-212
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• 1995

A strain BL-29, which produces a extracellular lytic enzyme on E. coli was isolated from the soil. The strain was identified as belonging to the genus Bacillus sp. The lytic enzyme was purified to homogeneity by ion exchange chromatography and gel filtration. Specific activity of the purified enzyme was 28, 850 U/mg protein and yield of the enzyme was 5$%$. The purified enzyme showed a single band on SDS-PAGE and its molecular weight was estimated to be 31, 000 by SDS-polyacrylamide gel electrophoresis and gel filtration column chromatography. The optimum temperature and pH were $55^{\circ}C$ and pH 10.0, respectively. The enzyme was stable at $45^{\circ}C$ but enzyme activity was reduced by up to 50$%$ when the temperature was raised to $55^{\circ}C$ for 15 min. Stable range of pH was from 5.0 to 11.0. but Enzyme activity was inhibited by lead-acetate, mercuric chloride, ethylene glycol-bis-[$\beta$-aminoethyl ether]-N, N, $N^1, $N^1$-tetraacetic acid (EGTA), and ethylenediamine tetraacetic acid (EDTA), but not affected considerably by treatment with other chemical reagents.

• Journal of Advanced Marine Engineering and Technology
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• v.27 no.3
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• pp.447-452
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• 2003

Crevice corrosion is localized form of corrosion usually associated with a stagnant solution on the micro-environmental level. Such stagnant micro environments tend to occur in crevices (shielded areas) such as those formed under gaskets washers insulation material. fastener heads. surface deposits. disbonded coatings. threads. lap joints and clamps. Crevice corrosion is initiated by changes in located electrochemical reaction within the crevice such as a) depletion of inhibitor in the crevice b) depletion of oxygen in the crevice c) a shift to acid conditions in the crevice and d) build-up of aggressive ion species (e.g chloride) in the crevice. In this study. the mechanism of crevice corrosion for Type 430 stainless steel is investigated undercondition that the size of specimen is $15{\times}20\{times}3mm$, in 1N $H_2SO_4$ + 0.05N NaCl solution. and the artificial crevice gap size of 3 x 0.2 x 15 mm. Crevice corrosion is measured under applied potential -300mV(SCE) to the external surface. The obtained result of this study showed that 1) the induced time for initiation of crevice is 750 seconds. 2) potential of the crevice was about from -320mV to -399mV. which is lower than that of external surface potential of -300mV It is considered that potential drop in the crevice is one of mechanisms for the crevice corrosion

• Bulletin of the Korean Chemical Society
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• v.26 no.1
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• pp.38-42
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• 2005

Bis(2-methylbenzaldehyde)butane-2,3-dihydrazone(TDSB) was used as new N-N Schiff's base which plays the role of an excellent ion carrier in the construction of a La(III) membrane sensor. The best performance was obtained with a membrane containing, 30% poly(vinyl chloride), 60% benzyl acetate, 6% TDSB and 4% sodium tetraphenyl borate. This sensor reveals a very good selectivity towards La(III) ions over a wide variety of cations, including alkali, alkaline earth, transition and heavy metal ions. The proposed electrode exhibits a Nernstian behavior (with slope of 19.8 mV per decade) over a wide concentration range (1.0 ${\times}$ 10$^{-5}$-1.0 ${\times}$ 10$^{-1}$ M). The detection limit of the sensor is 7.0 ${\times}$ 10$^{-6}$ M. It has a very short response time, in the whole concentration range ($\sim$5 s), and can be used for at least twelve weeks in the pH range of 3.0-9.4. The proposed sensor was successfully applied as an indicator electrode for the potentiometric titration of a La(III) solution, with EDTA. It was also successfully applied in the determination of fluoride ions in three mouth wash preparations.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.8
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• pp.829-836
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• 2005

In this study, the characteristics of atrazine decompositon with photo-chemical oxidation process was investigated by the oxidation products analysis. The main products of the process were OIET(2-hydroxy-4-ethylamino-6-isopropylamino s-triazine), OIAT(2-hydroxy-4-amino-6-isopropylamino s-triazine) and OAAT(2-hydroxy-4,6-diamino-s-triazine), resulting i n dechlorination or hydroxylation as the main mechanism of the photo-chemical oxidation process. Through the material balance analysis of TOC and chloride ion in the aqueous solution, it was concluded that mineralization of the atrazine was not occurred but the dechlorination of atrazine had been completed.

• Journal of Photoscience
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• v.4 no.1
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• pp.17-21
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• 1997

The photochemical reactions of the neat chloroform and the aqueous chloroform in the absence (saturated with argon) and presence of 02 (saturated with air or oxygen) have been investigated using 184.9 nm UV light. The irradiation of the deoxygenated neat chloroform causes the formation of hexachloroethane, pentachloroethane, and 1, 1, 2, 2-tetrachloroethane. The initial quantum yields of the products were determined to be 6.37 x 10$^{-4}$, 4.04 x 10$^{-4}$ and 1.76 x 10$^{-4}$, respectively. In the irradiation of aqueous chloroform, chloride ion was also formed along with the products listed above and 1, 1, 2, 2-tetrachloroethane was the predominant product among the chlorinated organic products, which contrasts to the case of the neat chloroform. The presence of oxygen during the irradiation of aqueous chloroform had an effect on the yield of the products. With increasing the concentration of oxygen, the formation of the products was decreased. Probable reaction mechanisms for the photochemical reaction were presented on the basis of products analysis.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2006.05a
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• pp.167-171
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• 2006

In this study, for the establishment of the performance evaluation methods of repair material and method for reinforced concrete structure and the quality control standards of durability recovery method, the quantitative exposure data by exposure experiment under the coastal and normal atmosphere environment is accumulated and analyzed. Investigating and evaluating the result of exposure experiment during 30 months of exposure age under the coastal and normal atmosphere environment, carbonation depth and chlorideion penetration depth very little penetrated than cover depth. It seems reasonable to conclude that main cause of corrosion of reinforcing bar are inner chloride-ion and macro cell from the result of corrosion area and corrosion velocity. Accordingly, it is considered that fundamental data on the performance evaluation and quality control standards of repair material and method could be presented through continuous exposure test in the future.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2005.11a
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• pp.29-32
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• 2005

Recently, for the problem solution of demand and supply imbalance of fine aggregate due to the shortage of natural fine aggregate and the environment regulation on sea sand extraction in the construction field, the studies for the application of recycled fine aggregate using waste concrete are being progressed versatilely. The purpose of this study is to investigate quality of recycled fine aggregate manufactured by drying manufacturing system which is the manufacture method of high duality recycled fine aggregate, and to analyze on thehardened and durability properties of recycled concrete using it. Therefore it is to present the fundamental data for application and utilization of recycled concrete. The results of this study are as follows; Quality of recycled fine aggregate by drying manufacturing system is improved, and compressive strength, carbonation depth and chloride ion penetration depth of recycled concrete using high quality recycled fine aggregate are similar to those of normal concrete using natural and crashed sand. But, resistance to $H_{2}SO_{4}$ show decreased somewhat.

• Journal of the Korean Ceramic Society
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• v.45 no.11
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• pp.719-724
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• 2008

Every concrete structure should continue to perform its intended functions such as to maintain the required strength and durability during its lifetime. Deterioration of the concrete structure, however, occurs more progressively from the outside of the concrete exposed to severe conditions. Main deteriorations in concrete structures result from carbonation, chloride ion attack and frost attack. Concrete can therefore be more durable by applying surface protection to increase its durability using impregnants, which are normally classified into two large groups in polymeric and silicate materials. Concrete impregnants are composed of silanes and alkali silicates (sodium, potassium and lithium silicate). Thus, this study is concerned with elevating the carbonation and Cl- penetration resistance of concrete structures by applying alkali silicate hydrophilic impregnants including lithium and potassium silicates. From the experimental test results, lithium and potassium silicates produced a good improvement in carbonation resistance and are expected to be used as hydrophilic impregnants of concrete structures.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2007.04a
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• pp.3-17
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• 2007

This paper provides an introduction to life-span simulation and numerical approach to support the performance design processes of reinforced concrete structures. An integrated computational system is proposed for life-span simulation of reinforced concrete. Conservation of moisture, carbon dioxide, oxygen, chloride, calcium and momentum is solved with hydration, carbonation, corrosion, ion dissolution. damage evolution and their thermodynamic/mechanical equilibrium. Coupled analysis of mass transport and damage mechanics associated with steel corrosion is presented for structural performance assessment of reinforced concrete. Multi-scale modeling of micro-pore formation and transport phenomena of moisture and ions are mutually linked for predicting the corrosion of reinforcement and volumetric changes. The interaction of crack propagation with corroded gel migration can also be simulated. Two finite element codes. multi-chemo physical simulation code (DuCOM) and nonlinear dynamic code of structural reinforced concrete (COM3) were combined together to form the integrated simulation system. This computational system was verified by the laboratory scale and large scale experiments of damaged reinforced concrete members under static loads, and has been applied to safety and serviceability assessment of existing structures. Based on the damage details predicted by the nonlinear finite element analytical system, the life-span-cost of RC structures including the original construction costs and the repairing costs for possible damage during the service life can be evaluated for design purpose.

• Corrosion Science and Technology
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• v.7 no.1
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• pp.35-39
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• 2008

We have been studying application of electrochemical noise (Fluctuation) analysis for localized corrosion. Foils of Zinc, Aluminum and Magnesium were used as specimens for electrochemical cell simulating localized corrosion. These specimens were dipped in sodium chloride solutions adjusted to each exponent of Hydrogen ion concentration (pH) condition of 5.5, 10, 12 respectively. Time variations of potential and current were measured in those solutions, and simultaneously the surfaces of specimens were observed using microscope with television monitor. Two types of electrochemical cells were arranged for experiments simulated localized corrosion. The fluctuations on trendy component of short-circuited potential and short-circuited current were appeared in synchronization. It was seemed that these fluctuations result from hydrogen evolution on the aluminum active site in the crevice from the microscopic observation. In the case of zinc and magnesium, fluctuations appeared on the trendy component of the corrosion potential. Two types fluctuation were detected. First one is the fluctuation varied periodically. The second one is the random fluctuation. It was seemed that these fluctuations result from generation of corrosion products and hydrogen evolution on the active site in the crevice of zinc and magnesium from the microscopic observation.