• Korean Journal of Environmental Biology
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• v.20 no.3
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• pp.256-262
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• 2002

This research investigated experimentally on the population growth in the aquatic microcosm with the wastewater of plating factory. The purpose of this study was to evaluate the effect of culture conditions of the characteristic growth pattern of the examined species. Population of the system is consists of three organisms; Chlorella vulgaris as a producer, Cyclidium glaucoma as a consumer and Pseudomonas putida as a decomposer. The different growth patterns of each population are followed by surfactant type; Especially C. glaucoma was sensitive, Ch. uvlgaris was maintained population size stably even at high level of surfactant and p. putida was not significantly affected. After treatment of waste water from plating factory, it began to be affected at 1.0% solution treatment to Ch. vulgaris which the cell number was decreased prominently after 2 days, and C. glaucoma was disappeared at 2.5% solution treatment. P. putida was showed increasing pattern according to treatment concentration, at 2.5% solution and population size grew double. The result from current microcosm study indicates that this model system can be applied to environmental assessment method for various pollutants.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.16
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• pp.6773-6779
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• 2014

Background: Nanotechnology opens new applications in many fields including medicine. Among all metallic nanoparticles, silver nanoparticles (silver NPS) have proved to be the most effective against a large variety of organisms including toxic cyanobacteria. Materials and Methods: Silver NPs were biosynthesized in vivo with different alga species namely, Spirulina piatensis, Chlorella vulgaris and Scenedesmus oh/iquus following two scenarios. First: by suspending a thoroughly washed algae biomass in 1 mM aqueous $AgN0_3$ solution. Second: by culturing them individually in culture media containing the same concentration of $AgN0_3$. Silver NPs were characterized using UV-Vis spectroscopy, transmission electron microscopy (TEM), energy dispersive analysis (EDX) and Fourier transform infra-red (FfIR) spectroscopy. The biosynthesized silver NPs were tested for cytotoxic activity against a cancer promoter cyanobacteruim Microcystis aeruginosa, considering effects on cell viability and chlorophyll content. Results: The surface plasmon band indicated the biosynthesis of silver NPs at ~400 nm. Transmission electron microscopy (TEM) revealed that the silver NPs had a mean average size below 100 nm. Energy-dispersive analysis X-ray (EDX) spectra confirmed the presence of silver element. FfIR spectral analyses suggested that proteins and or polysaccharides may be responsible for the biosynthesis of silver NPs and (-COO-) of carboxylate ions is responsible for stabilizing them. The toxic potentialities ofthe biosynthesized silver NPs against the cancer promoter cyanobacterium, Microcystis aeruginosa showed high reduction in viable cells count and the total chlorophyll content. Conclusions: The potential activity of the biosynthesized silver NPs from the studied algae species against Microcystis aernginosa cells is expected to be mainly mediated by the release of silver ions (Ag+) from the particle surface and bioactive compounds as indicated by FfIR analysis.