• Journal of Ginseng Research
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• 제20권1호
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• pp.88-95
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• 1996

The effects of media, incubation time, temperature and pH on production of conidia and chlamydospore of Cylindrocarpon destructans (Zinssm.) Scholen causing root rot of Panax ginseng were studied. Microconidia of the pathogen were abundantly produced on V-8 juice agar as a solid substrate with 5.73(log conidia/mm2) and in V-8 broth as a liquid substrate with 6.65 (log conidia/ml) among media tested. No difference was observed on the length of microconidia produced from the media with a range of 9.50∼11.38 $\mu\textrm{m}$. However, tryptic soy agar produced the broadest microconidia (average 5.00 $\mu\textrm{m}$) among the media tested. All the media produced chlamydospores In a range of 1.06∼4.37 (log chlamydospores/mm2) without a significant difference in number, while V-8 juice agar produced the bigger one (18.39 $\mu\textrm{m}$ in diameter) as compared to the tested media. The fungus began to sporulate conidia after three days of incubation and reached maximum at the 8th day. It seemed to be in a stationary phase until 30 days of incubation but was decreased thereafter. Chlamydospore was produced at 4th day after incubation. Maximum production was observed at 8th day and the number seemed to be maintained during the observation period. Both conidia and chlamydospore of the pathogen were able to be spoluated at 10∼25$^{\circ}C$. However, optimum temperatures of conidia and chlamydospore formation were 15∼25$^{\circ}C$ and 10∼20$^{\circ}C$, respectively. C. destrmtans produced conida with an wide range of pH from 3.3 to 8.0 and chlamydospore from 2.8 to 8.0. Number of conidia was increased with an increase of pH up to 4.0. There was no significant difference in the number between 4.0 to 8.0. It seemed to have two optimum pH ranges, 3.3∼4.0 and 7.1∼8.0 for the chlamydospore formation.

• Journal of Ginseng Research
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• 제22권4호
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• pp.304-309
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• 1998

Incubation condition affecting the chlamydospore formation and isolation from mycelia and conidia of Cylindrocarpon destructanse (isolate ACY-9701), isolated from the root rot lesion of the American ginseng (Panax quinquefolium) was investigated. Chlamydospores were formed from mycilia but not from conidia on the Czapek-Dox agar without carbon or nitrogen source after 20 days incubation at 2$0^{\circ}C$. In the medium added with nitrogen and carbon sources, immatured chlamy-dospore-like cells were formed from microconidia and mycelia as well. Immatured chlamydospore-like cells were formed from mycelia as well as microconidia In corn, kidney bean, and pea root extracts after 20 days incubation at 20"C, while typical chlamydospores were formed from both of them in the root extract of Panax quinquefolium. The 3.6 log chlamydospore/mm" was converted from microconidia in the medium, which was equal to 2.5% conidia formed. Under the light condition (251.1 pmol/m" sec, 12 hrs dark and light cycle), 4.2 log/mm" of chlamydospores were converted from interracially or terminal cells of macroconidia, which was 4.0% of macroconidia produced on Potato dextrose agar (PDA). When mycelia and microconidia were stored at -7$0^{\circ}C$ for 32 days and incubated on PDA after thawing at room temperature to isolate chlamydospores from them, microconidia and mycelia were still alive. Meanwhile, microconidial lysis was found after heating them at 32$^{\circ}C$ for 7 days, but the chlamydospores converted from macroconidia were not lysed up to 13 days at 32"C. to 13 days at 32"C.ot;C.

• 한국식물병리학회지
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• 제13권1호
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• pp.30-36
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• 1997

The effects of carbon and nitrogen sources on the mycelial growth and sporulation of microconidia and chlamydospores of five isolates of Cylindrocarpon destructans (Zinssm.) Scholten causing root rot of Panax ginseng were studied. For the carbon sources, fructose, glucose, maltose, and sucrose in Czapek-Dox broth showed good mycelial growth of 178∼201 mg in dry weight compared with 64 mg of the control. The best carbon sources tested for conidial formation were sucrose and maltose with 2.75 and 3.03 log conidia/ml, respectively. For the nitrogen sources, aspartic acid, NaNO3, KNO3, arginine, threonine, and leucine increased mycelial growth of the fungi to 208∼231 mg in dry weight without significant difference (p=0.05) among them. Meanwhile the growth with cystine was poor (26.3 mg dry weight), and no conidium and chlamydospore were formed. Maximum microconidial formation was observed in the media with NaNO3 and KNO3 as 3.37 and 3.35 log conidia/ml, and for the chlamydospore formation the (NH4)2SO4-containing medium and the nitrogen-absent medium were the best as 3.40 and 3.57 log chlamydospores/ml, respectively. No conidium was found in the medium without nitrogen sources, in which chlamydospore formation increased 6 times more than in the nitrogen-amended medium. However, deletion of carbon source in the medium did not affect on the formation of conidia and chlamydospores of C. destructans.

• Mycobiology
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• 제44권1호
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• pp.63-65
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• 2016

Cylindrocarpon destructans causes root rot disease in ginseng and can survive for a long time, producing chlamydospores. We optimized conditions to induce chlamydospore production from the conidia of C. destructans, isolated from Korean ginseng. This will provide the basis for testing the efficacy of control agents targeting these chlamydospores.

• Mycobiology
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• 제46권4호
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• pp.396-406
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• 2018

A newly isolated white rot fungal strain KU-RNW027 was identified as Trametes polyzona, based on an analysis of its morphological characteristics and phylogenetic data. Aeration and fungal morphology were important factors which drove strain KU-RNW027 to secrete two different ligninolytic enzymes as manganese peroxidase (MnP) and laccase. Highest activities of MnP and laccase were obtained in a continuous shaking culture at 8 and 47 times higher, respectively, than under static conditions. Strain KU-RNW027 existed as pellets and free form mycelial clumps in submerged cultivation with the pellet form producing more enzymes. Fungal biomass increased with increasing amounts of pellet inoculum while pellet diameter decreased. Strain KU-RNW027 formed terminal chlamydospore-like structures in cultures inoculated with 0.05 g/L as optimal pellet inoculum which resulted in highest enzyme production. Enzyme production efficiency of T. polyzona KU-RNW027 depended on fungal pellet morphology as size, porosity, and formation of chlamydospore-like structures.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.163-163
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• 2005

• 한국식물병리학회지
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• 제12권4호
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• pp.422-427
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• 1996

인삼뿌리썩음병균(Cylindrocarpon destructans)후막 포자의 형성은 CD(Czapek`s dox broth)와 SNY(Spezieller Nahrtoffarmer yeast extract broth)배지에서 진탕배양(2$0^{\circ}C$, 120 rpm)하면 양호하였다. 특히 CD배지에서 2$0^{\circ}C$로 30일간 진탕배양하면 5.99 log 후막포자/ml로 형성되었다. 후막포자의 발아율은 12.9~23.3%로 CD배지와 SNY배지에서 좋았으며 발아적온은 5~1$0^{\circ}C$였고, 최적 pH는 6이었다. 그러나 2$0^{\circ}C$이상에서는 전혀 발아하지 않았으며 pH 7.0이상에서는 발아율이 급속히 저하되었다. 인삼추출물 3%, GA 10 ppm, IAA 10 ppm, NOVOZYM\ulcorner234 20 ppm을 처리 할 경우 대조구에 비해 발아율이 증가하였다. 특히 NOVOZYM\ulcorner234 20 ppm 처리구는 발아율이 무처리에 비해 2배 증가하였다. CD배지(pH5)에 NOVOZYM\ulcorner234 20 ppm과 GA 10 ppm을 조합처리하고 5$^{\circ}C$로 정치배양 할 때 후막포자의 발아율은 49.4%로 가장 높았다.

• Journal of Ginseng Research
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• 제27권4호
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• pp.195-201
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• 2003

인삼 뿌리썩음병균 Cylindrocarpon destructans의 후막포자형태에 대해서 광학현미경 및 전자현미경을 사용하여 조사하였다. 전형적인 C. destructans의 후막포자는 균사로부터 형성되었으나 분생포자에서는 생성되지 않았다. 그러나 pH 4로 조절된 Czapek Dox broth에서 2$0^{\circ}C$, 12일간 배양하였을 때는 소형분생포자가 미성숙한 후막포자 유사세포로 변형되었다. 후막포자는 potato dextrose agar 및 V-8 Juice agar 그리고 질소원이 결제된 Czapek Dox broth배지에서 2$0^{\circ}C$, 16∼20일 배양으로 생성되었고, 생성된 후막포자는 황갈색 혹은 적갈색을 띄었으며 균사의 중간이나 말단 부위에서 한 개 혹은 사슬형태로 존재하였다. 후막포자는 직경이 11.3∼11.9 $\mu\textrm{m}$이었으며 외부에 1.5∼1.8 $\mu\textrm{m}$ 길이의 혹 같은 돌기가 여러 개 형성되어 표면이 울퉁불퉁 하였다.

• Journal of Ginseng Research
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• 제23권3호
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• pp.123-129
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• 1999

Potato dextrose agar(PDA), V-8 agar, Ginseng extract agar배지에 C. destnctam를 배양한 후 기중균사를 제거하고 25,000 Lux의 광처리로 대형분생포자 생성을 유도한 결과, 암처리에 비해 대형분생포자 생성이 $3.7\~8.1$배 촉진되었다. 위 공시 배지의 액체배지들의 경우에는 광처리에 의해 대형분생포자 생성이 암처리에 비해 $7.7\~18.0$배 촉진되었다. 광처리로서 대형분생포자를 가장 많이 생성한 배지는 PDA와 V-Bagar로 조사되었다. C. destnctans의 대형분생포자 생성에 미치는 광처리 조건중 기중균사 제거 효과를 검토하기 위해 C. destructans가 생육된 Potato dexoose agm에 기중균사를 제거하고 광량별 처리로 대형분생포자 생성량을 측정한 결과, 25,000Lux 의 광처리로 대형분생포자 생성량이 최고 1,585$macroconidia/mm^2$로 측정되어 3,000Lux 및 10,000Lux의 광처리 후 측정된 최고 생성량에 비해 각각 3.2배, 1.4배 많이 생성되었다. 그러나 기중균사 무제거구는 $3,000\~25,000$Lux의 광처리 조건에서 대형분생포자는 $20\~99\;macroconidia/mm^2$의 적은양이 생성되어 광처리시 기중균사 제거는 C. destructans의 대형 분생포자 생성을 촉진하는 것으로 나타났다. 대형분생포자를 구성하는 세포수는 기중균사의 제거 및 무제거와 광량별 처리에 관계없이 $1~3$개로 관찰되었고 2개의 세포로 구성된 것이 $69.4\~100\%$로 대부분을 차지하였다. 광처리로부터 얻어진 대형분생포자는 광처리 6~7일 경과후 부터 분해되기 시작하였다. C. destructans의 기중균사 무제거 상태에서 광처리를 55일간 지속한 결과 광량증가에 따라 균사체가 변형된 후막포자 형성이 촉진되어, 25,000Lux광처리구에서 후막포자 생성량이 1,285$chlamydospres/mm^2$로서 3,000 및 10,000Lux의 경우에 비해 각각 2.8, 1.2배 많이 생성되었다. C. destructans의 기중균사를 제거한 상태로 광을 처리한 경우에는 균사체변형 후 막포자 형성이 더욱 촉진되어 기중균사 제거후 3,000Lux, 10,000Lux, 및 25,000Lux의 각 광처리에서는 기중균사 무제거구의 각 광처리 대조구들에 비해 후막포자가 각각 1.9, 2.5, 1.4배 많이 생성되었다. 균사체변형 후막포자 chain내 세포수는 기중균사 제거 혹은 무제거구의 광 및 암처리에 관계없이 1~8 개이었고 1개의 단독세포로 구성된 것이 $34.2\~58.9\%$로 가장 많았다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.132.3-133
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• 2003

Leaf blight of kudzu ( Pueraria lobata ) was found in Jeonbuk province in 2002. The main symptoms appeared as leaf blight and showed yellowing and wilting. The causal pathogen of the leaf blight was isolated from symptomed kudzu leaf and produced white to cream, usually floccose mycelium. It readily formed reddish orange mycelium on PDA. It produced typical microconidia and macroconidia. The microconidia were the reniform. The macroconidia were wide, slightly curved, usually 3 to 4 septate and size was 45 ∼ 85 ${\times}$ 5 ∼ l0$\mu\textrm{m}$. The pathogen produced chlamydospore singly on short hyphal branches within 2 to 3 weeks, which was hyaline, globose, and smooth walled. The pathogen was, therefore, identified as Fusarium solani based on cultural and morphological characters. This is the first report on the leaf blight of kudzu caused by Fusarium solani in Korea.