• Microbiology and Biotechnology Letters
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• v.14 no.6
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• pp.433-439
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• 1986

Abundant amount of Chiamydia trachomatis could be amplified in mammalian McCoy cells and purified using descontinuous Uroarafin gradient centrifugation. As a chemical means io increase the Chlamydia trachomatis inclusions in McCoy cells IUdR treatment was found to be more effective than the cycloheximide treatment and was recommendanble for the proliferation of Chlamydia trachomatis. Centrifugation promoted Chlamydia trachomatis adhesion to McCoy cell surface, and maximal percentage of infected cells was obtained at about 3000g. The purified Chlamydia trachomatis could be kept in SPG solution for 48 hours at +4$^{\circ}C$ but for longer storage freezing to -7$0^{\circ}C$ was necessary.

• Journal of Microbiology
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• v.41 no.2
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• pp.137-143
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• 2003

The present study was performed to identify pathogenic factors of Chlamydia trachomatis, which invade the host cell membrane. We prepared monoclonal antibody against C. trachomatis and searched for pathogenic factors using this antibody, and subsequently identified the surface components of the elementary body of C. trachomatis, i.e., major outer membrane protein (MOMP), lipopolysaccharide (LPS), and two other surface exposure proteins. These proteins are believed to be important in the pathogenesis of host cell chlamydial infection. Additionally, to identify factors related to the host cell and C. trachomatis, we prepared C. trachomatis infected and non-infected McCoy cell extracts, and reacted these with anti-chlamydial LPS monoclonal antibody. We found that anti-chlamydial LPS monoclonal antibody reacted with a 116 kDa proteinaceous McCoy cell membrane component.

• The Journal of the Korean Society for Microbiology
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• v.22 no.3
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• pp.197-208
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• 1987

Chlamydia trachomatis has now shown that this interesting intracellular parasite is a cause of nongonococcal urethritis, infantile pneumonia, pelvic inflammatory disease and epididymitis, in addition to lymphogranuloma venerum and inclusion conjunctivitis. There are several diagnostic methods for C. trachomatis, but the method using monoclonal antibody is the most sensitive and specific. The hybride cell were prepared by fusion of myeloma cell($P_3X_{63}\;Ag_8{\cdot}V_{653}$) of mouse and lymphocyte of mouse(BALB/c) that were immunized with formalin killed C. trachomatis serotype D. The cell mixtures after fusion were dispensed into 640 wells of the 96 well culture plates and continuously cultured in HAT medium for 2 weeks. The supernatants of culture media in 83(13%) wells were reacted with C. trachomatis, which were determined by enzyme-linked immunosorbent assay in 96 well microplate. The clones that secreted antibody to C. trachomatis were cloned by limiting dilution. Only six monoclones secreted antibody to C. trachomatis. The antibody titer of ascitic fluid that collected from same BALB/c mice bearing hybridoma cells was above 1:100,000. These monoclonal antibodies that were IgG reacted with elementary and reticulate bodies of all serotypes(Ba, D, E, F, G, H, J and LGV type-I) using ELISA and indirect immunofluorescence stain, but there were no cross reaction with other bacteria(coagulase negative Staphylococcus, Proteus and E. coli). We concluded these six monoclones secreted the same monoclonal antibody to C. trachomatis. The sensitivity and specificity of the monoclonal antibody compared with Microtrak(confirmatory test of C. trachomatis, Syva) was 100%, respectively.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.8
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• pp.3701-3704
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• 2012

Background: Cervical cancer is one of the most common cancers in developing countries and the second most common type of cancer in women globally. Several recent studies suggested a co factor role for Chlamydia trachomatis in pathogenesis of cervical cancer. This study aimed to evaluate existence of C. trachomatis DNA in pathologic blocks of patients with cervical cancer. Materials and methods: Seventy-six formaldehyde fixed paraffin embedded tissue specimens from patients with histologically proven history of cervical cancer as well as 150 blocks from healthy peoples were included in the present study. Thin slices were prepared from selected blocks followed by deparaffinization and DNA extraction; the presence of C. trachomatis DNA was examined by Taq Man real-time PCR. Results: Our TaqMan real time PCR assay with cervical specimens of patients with cervical cancer showed that there was no C. trachomatis DNA. Also, we found three positive specimens among our control group. Conclusion: It seems that based on results obtained from the specimens examined in the present study, there is no association between the presence of C. trachomatis DNA in cervical specimens and cervical cancer.

• The Journal of the Korean Society for Microbiology
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• v.21 no.2
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• pp.261-270
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• 1986

A total of 339 urethral, vaginal swab and eye discharge materials from the out-patients in the hospitals of Seoul area was microbiologically collected for the detection of Chlamydia trachomatis infection during May through August, 1985. McCoy cell culture system (MCC) and enzyme-likned immunosorbent assay (EIA) methods were employed in this study as the tools for the detection of C. trachomatis, and the detectabilities of two methods were compared. The results obtained in this study are summarized as follows: 1. The positive rate of C. trachomatis in 339 swab specimens was 18.6%, and the rate in females (20.1%) was much higher than that in males (7.1%). 2. The positive rate of C. trachomatis infection the prostitutes was the highest (24.2%), and the rate in the eye discharge specimens obtained from the new barns was 12.8%. 3. The positive rates of C. trachmoatis infection detected in the specimens from the patients with vaginitis and leucorrhea, with infertility, with cystitis and with nongonococcal urethritis were 17.2%, 21.9%. 18.0% and 7.1%, respectively. 4. The positive rate of C. trachomatis infection in 20-25 age group was 30.5%. This rate was the highest among the other age groups. 5. The positive rate of C. trachomatis infection in the randomly screened 89 swab specimens by EIA (30.3%) was much higher than the rate detected by MCC (18.6%). 6. The positive rate of C. trachomatis infection in females detected by EIA was also much higher than in males, and the 20-25 age group showed the highest positive rate as compared to the other age groups. 7. Sensitivity and specificity of EIA for the detection on C. trachomatis were 100% and 88.6%, respectively, in case that MCC was regarded as perfect method. In summarizing the above results, it is known that considerable cases with genital diseases and with eye discharges were associated with C. trachomatis, and that EIA method is recommendable for the detection of C. trachomatis especially in the specimens swabed from the genital tracts.

• Clinical and Experimental Pediatrics
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• v.51 no.7
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• pp.729-735
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• 2008

Purpose : Chlamydia trachomatis is one of the most common sexually transmitted diseases and is also a cause of pneumonia in infants. Respiratory infections by respiratory viruses are also common for infants. The objectives of this study were to identify the clinical manifestations and to determine the prevalence of C. trachomatis respiratory infections and coinfections by respiratory viruses in infants younger than 6 months of age. Methods : For this study, we enrolled 6 months or younger infants who were admitted to the Dankook University Hospital between January 2002 and July 2007, with respiratory symptoms. Nasopharyngeal aspirates or throat swabs were collected within s d of hospitalization and C. trachomatis was detected using polymerase chain reaction (PCR). Patients who tested positive underwent multiplex PCR for respiratory viruses. Results : A total of 690 patients underwent chlamydial PCR testing and 36 (5.2%) had positive results. Of the 36, 28 (78%) were male; 30 were vaginally delivered. From the 36 patients positive for C. trachomatis, 26 underwent multiplex respiratory viral PCR; 12 were coinfected with viruses. Respiratory syncytial virus (RSV) was the most frequent pathogen that was detected in 6 patients. Increased C-reactive protein and fever were significant in patients coinfected with respiratory viruses. Conclusion : C. trachomatis can infected in infants delivered by cesarean section as well as in 6 months old or younger infants. Infant with C. trachomatis respiratory infections can also be coinfected with respiratory infection also coinfected with respiratory viruses. Further studies are needed to better understand the prevalence rates of the this infection and its coinfection rate with respiratory viruses.

• The Journal of the Korean Society for Microbiology
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• v.21 no.3
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• pp.393-397
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• 1986

Chlamydia trachomatis is one of the most common cause in non-gonococcal urethritis. There are several diagnostic methods for Chlamydia trachomatis; culture method using McCoy cell, enzyme immunoassay and direct immunofluorescence staining etc. We have studied a sensitivities of culture, chlamydiazyme and direct immunofluorescence staining(DIF). 85 male patients previously conformed to non-gonococcal urethritis have been selected in this study. Three samples were concurrently collected in the same patient. First sample was used to inoculation in McCoy cell, 2'nd sample was used to Chlamydiazyme test and 3'rd sample was used to direct immunofluorescence staining method. The results are following. 1. All culture, Chlamydiazyme and DIF positive cases are 15/85(17.7%). 2. Culture and Chlamydiazyme positive but DIF negative cases absent. 3. Culture and DIF positive, but Chlamydiazyme negative cases are 2/85(2.4%). 4. Chlamydiazyme and DIF positive, but culture negative cases are 9/85(10.6%). 5. Culture positive, but Chlamydiazyme and DIF negative cases are 6/85(7.1%). 6. Chlamydiazyme positive, but culture and DIF negative cases are 7/85(8.2%). 7. DIF positive, but culture and Chlamydiazyme negative cases are 3/85(3.5%). 8. All culture, Chlamydiazyme and DIF negative cases are 43/85(50.1%). In summarized, anyone positive cases of culture, Chlamydiazymc and DIF are 42/85(49.9%).

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.3
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• pp.1497-1500
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• 2014

Cervical cancer continues to be an important public health problem in Thailand. While the high risk human papillomavirus (HPV) types have been established as the principle causative agent of both malignancies and the precursor lesions, cervical intraepithelial neoplasia (CIN), other factors may also be involved like other sexually transmitted diseases, as well as smoking. Chlamydia trachomatis is an obligate intracellular Gramnegative bacterium which has a tendency to cause chronic infection featuring inflammation and therefore might be expected to increase the risk of cervical cancer. In the present nested case-control study, 61 cases of cervical cancer and 288 matched controls with original serum samples were identified from the Khon Kaen Cohort, established in the North-East of Thailand, by linkage to the Khon Kaen population based cancer registry. C. trachomatis specific IgG antibodies at recruitment were measured by microimmunofluorescence and assessed for association with cervical cancer using STATA release10. No significant link was noted either with all cancers or after removal of adenocarcinomas. The results suggest no association between Chlamydia infection and cervical cancer development in North-East Thailand, but possible influencing factors must be considered in any future research on this topic.

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.204.2-204
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• 1994

No Abstract, See Full Text

• Advances in pediatric surgery
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• v.17 no.2
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• pp.188-192
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• 2011

Fitz-Hugh-Curtis 증후군은 골반 내 염증성 질환을 가진 환자의 직접적 간 실질 침범이 없는 간피막 염증에 의한 간주위염으로, 1930년대에 Thomas Fitz-Hugh와 Arthur Curtis에 의해 보고되었다. Neisseria gonorrhea나 Chlamydia trachomatis에 의해 발병하며, 항생제로 치료되는 양성 성교전파질환이다. 대부분 가임기의 젊은 여성에서 발견되지만, 15세의 청소년에서 진단된 증례가 있어 보고하는 바이다. 15세 여자 환자가 1개월 전부터 발생한 간헐적인 우상복부와 하복부의 통증을 주소로 내원하였다. 환자는 한 달 전 남자친구와 첫 성교를 한 이후, 5일 전까지 10여 차례정도 성교를 하였다. 사회력 상 고등학교 1학년 생이고, 월경 주기는 28-30일로 규칙적이었다. 내원 당일 시행한 임신 반응 검사는 음성이었다. 시행한 복부전산화단층촬영에서 우측 간엽의 가쪽 부분이 동맥기 조영증강을 보이며 골반내감염을 동반하고 있었다. 부인과 검진 상 질경부 면봉 검사에서 Chlamydia trachomatis 양성소견을 보였으며, 소변배양검사에서 Neisseria gonorrhea가 동정되었다. Fitz-Hugh-Curtis 증후군 진단 하에 4주간 doxycycline과 metronidazole의 경구용 항생제 복용 후 호전되었다.