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http://dx.doi.org/10.7314/APJCP.2012.13.8.3701

Lack of Association between Chlamydia trachomatis Infection and Cervical Cancer - Taq Man Realtime PCR Assay Findings  

Farivar, Taghi Naserpour (Cell and Molecular Research Center, Qazvin University of Medical Sciences)
Johari, Pouran (Cell and Molecular Research Center, Qazvin University of Medical Sciences)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.8, 2012 , pp. 3701-3704 More about this Journal
Abstract
Background: Cervical cancer is one of the most common cancers in developing countries and the second most common type of cancer in women globally. Several recent studies suggested a co factor role for Chlamydia trachomatis in pathogenesis of cervical cancer. This study aimed to evaluate existence of C. trachomatis DNA in pathologic blocks of patients with cervical cancer. Materials and methods: Seventy-six formaldehyde fixed paraffin embedded tissue specimens from patients with histologically proven history of cervical cancer as well as 150 blocks from healthy peoples were included in the present study. Thin slices were prepared from selected blocks followed by deparaffinization and DNA extraction; the presence of C. trachomatis DNA was examined by Taq Man real-time PCR. Results: Our TaqMan real time PCR assay with cervical specimens of patients with cervical cancer showed that there was no C. trachomatis DNA. Also, we found three positive specimens among our control group. Conclusion: It seems that based on results obtained from the specimens examined in the present study, there is no association between the presence of C. trachomatis DNA in cervical specimens and cervical cancer.
Keywords
Chlamydia trachomatis DNA; real-time PCR; cervical cancer;
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1 Anonymous (2010). Disease Control and Prevention, and National Cancer Institute (2010). Available at:http://www.cdc.gov/uscs.
2 Bosch FX, de Sanjosé S (2007). The epidemiology of human papillomavirus infection and cervical cancer. Dis Markers, 23, 213-27.   DOI   ScienceOn
3 Carozzi F, Ronco G, Gillio-Tos A, et al (2012). Concurrent infections with multiple human papillomavirus (HPV) types in the New Technologies for Cervical Cancer (NTCC) screening study, Working Group. Eur J Cancer, 48, 1633-7.   DOI
4 Castle PE, Escoffery C, Schachter J, et al (2003). Chlamydia trachomatis, herpes simplex virus 2, and human T-cell lymphotrophic virus type 1 are not associated with grade of cervical neoplasia in Jamaican colposcopy patients. Sex Transm Dis, 30, 575-80.   DOI   ScienceOn
5 Dahlstrom LA, Andersson K, Luostarinen T, et al (2011). Prospective Seroepidemiologic Study of Human Papillomavirus and Other Risk Factors in Cervical Cancer. Cancer Epidemiol Biomarkers Prev, 20, 2541-50.   DOI
6 Daling JR, Madeleine MM, McKnight B, et al (1996). The relationship of human papillomavirus-related cervical tumors to cigarette smoking, oral contraceptive use, and prior herpes simplex virus type 2 infection. Cancer Epidemiol Biomarkers Prev, 5, 541-8.
7 Deluca GD, Marin HM, Schelover E, et al (2006). Chlamydia trachomatis and papillomavirus infection in women with cytohistological abnormalities in uterine cervix. Medicina (B Aires), 66, 303-6.
8 Du P, Lemkin A, Kluhsman B, et al (2010). The roles of social domains, behavioral risk, health care resources, and chlamydia in spatial clusters of US cervical cancer mortality: not all the clusters are the same. Cancer Causes Control, 21, 1669-83.   DOI
9 Farivar TN, Johari P, Shafei S, et al (2012). Lack of association between herpes simplex virus type 2 infection and cervical cancer-Taq Man Real-time PCR assay findings. Asian Pac J Cancer Prev, 13, 339-42.   DOI   ScienceOn
10 Finan RR, Musharrafieh U, Almawi WY, et al (2006). Detection of Chlamydia trachomatis and herpes simplex virus type 1 or 2 in cervical samples in human papilloma virus (HPV)-positive and HPV-negative women. Clin Microbiol Infect, 12, 927-30.   DOI
11 Jensen KE, Munk C, Sparen P, et al (2011). Women's sexual behavior.Population-based study among 65,000 women from four Nordic countries before introduction of human papillomavirus vaccination. Acta Obstet Gynecol Scand, 90, 459-67.   DOI
12 Klomp JM, Boon ME, Dorman MZ, (2010). Trends in inflammatory status of the vaginal flora as established in the Dutch national screening program for cervical cancer over the last decade. Acta Cytol, 54, 43-9   DOI
13 Kwasniewska A, Korobowicz E, Zdunek M, et al (2009). Prevalence of Chlamydia trachomatis and herpes simplex virus 2 in cervical carcinoma associated with human papillomavirus detected in paraffin-sectioned samples. Eur J Gynaecol Oncol, 30, 65-70.
14 Madeleine MM, Anttila T, Schwartz SM, et al (2007). Risk of cervical cancer associated with Chlamydia trachomatis antibodies by histology, HPV type and HPV cofactors. Int J Cancer, 120, 650-5.   DOI
15 Magaldi TG, Almstead LL, Bellone S, et al (2012). Primary human cervical carcinoma cells require human papillomavirus E6 and E7 expression for ongoing proliferation. Virology, 5, 114-24.
16 Naucler P, Chen HC, Persson K, et al (2007). Seroprevalence of human papillomaviruses and Chlamydia trachomatis and cervical cancer risk: nested case-control study. J Gen Virol, 88, 814-22.   DOI   ScienceOn
17 Menczer J (2003) The low incidence of cervical cancer in Jewish women: Has the Puzzle Finally Been Solved?, 5,120-12
18 Miller WC, Ko EM, ONE AUTHOR, et al (2011). Does chlamydial infection increase the risk of cervical dysplasia? Sex Transm Infect, 87, 366-7   DOI
19 Naserpour Farivar T, Johari P, Pahlavan AA, et al (2012). Agreement rate of rapid urease test, conventional PCR, and Scorpion real-time PCR in detecting Helicobacter pylori from tonsillar samples of patients with chronic tonsillitis. J Glob Infect Dis, 4, 38-42.   DOI
20 Paavonen J, Karunakaran KP, Noguchi Y, et al (2003). Serum antibody response to the heat shock protein 60 of Chlamydia trachomatis in women with developing cervical cancer. Am J Obstet Gynecol, 189, 1287-92.   DOI
21 Quint, KD, de Koning MNC, Geraets DT, et al (2009) Comprehensive analysis of Human Papillomavirus and Chlamydia trachomatis in in-situ and invasive cervical adenocarcinoma. Gynecologic Oncology, 114, 390-4   DOI   ScienceOn
22 Safaeian M, Quint K, Schiffman M, et al (2010). Chlamydia trachomatis and risk of prevalent and incident cervical premalignancy in a population-based cohort. J Natl Cancer Inst, 102, 1794-804.   DOI   ScienceOn
23 Samoff E, Koumans EH, Markowitz EL, et al (2005). association of chlamydia trachomatis with persistence of high-risk types of human papillomavirus in a cohort of female adolescents. Am J Epidemiol, 162, 668-75.   DOI
24 Steben M, Duarte-Franco E (2007). Human papillomavirus infection: epidemiology and pathophysiology. Gynecol Oncol, 107, 2-5.   DOI
25 Saveleva NV, Zagryadskaya YE, Klimashevskaya SV, et al (2010). Scanning diagnostically significant antigenic regions of major Chlamydia trachomatis protein MOMP using series of overlapping recombinant proteins. Molecular Genetics. Microbiology and Virology, 24, 133-7.
26 Simonetti AC, Melo JH, de Souza PR, et al (2009). Immunological's host profile for HPV and Chlamydia trachomatis, a cervical cancer cofactor. Microbes Infect, 11, 435-42.   DOI
27 Smith JS, Bosetti C, Munoz N, et al (2004). Chlamydia trachomatis and invasive cervical cancer: a pooled analysis of the IARC multicentric case-control study.IARC multicentric case-control study. Int J Cancer, 111, 431-9.   DOI
28 Valadan M, Yarandi F, Eftekhar Z, et al (2010). Chlamydia trachomatis and cervical intraepithelial neoplasia in married women in a Middle Eastern community. East Med Health J, 16, 304-7.
29 Vidal AC, Murphy SK, Hernandez BY, et al (2011). Distribution of HPV genotypes in cervical intraepithelial lesions and cervical cancer in Tanzanian women. InfectAgent Cancer, 14, 20-5.
30 Wallin KL,Wiklund F,Luostarinen T, et al (2002) A populationbased prospective study of Chlamydia trachomatis infection and cervical carcinoma. Inter J Cancer, 101, 371-4.   DOI   ScienceOn
31 Zereu M, Zettler CG, Cambruzzi E, et al (2007) Herpes simplex virus type 2 and Chlamydia trachomatis in adenocarcinoma of the uterine cervix. Gynecol Oncol, 105, 172-5.   DOI