• Horticultural Science & Technology
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• v.28 no.3
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• pp.343-352
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• 2010

In order to understand the plant responses to salt stress (0, 50, and 100 mM NaCl), Chinese cabbage seedlings grown up to two leaf stages by hydroponic culture were used. Fresh and dry weight, chlorophyll (Chl), antioxidant materials, polyamine content, antioxidant enzyme activities, and inorganic ion level were evaluated. Fresh and dry weights of Chinese cabbage increased with the increase in salinity while the optimal growth occurred at 50 mM NaCl. The Chl a, total Chl, carotenoid content, and Chl a/b ratio increased by the 6 days after treatment with 100 mM NaCI; however, the Chl b content decreased. Glutathione increased in the root of Chinese cabbage for 6 days. Dehydroascorbate increased remarkably by day 6 caused by the salt stress in both leaf and the root. While ascorbate peroxidase increased, the activity of catalase and glutathione reductase decreased gradually in the first leaf for 6 days. The $Na^+$ content increased by 12.5-fold in the 3 days after treatment with 100 mM NaCI in the shoot, whereas the $Ca^{2+}$, $K^+$, and $Mg^{2+}$ content measured in the same treatment decreased by 43 to 57%. Spermidine content decreased as salinity increased, but spermine content increased. The growth promotion, glutathione and ascorbic acid content in Chinese cabbage were increased by low salt stress, and shortening of the cultivation period for growth increase of Chinese cabbage is expected.

• The Plant Pathology Journal
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• v.35 no.5
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• pp.521-529
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• 2019

Tomato yellow leaf curl China virus is a species of the widespread geminiviruses. The infection of Nicotiana benthamiana by Tomato yellow leaf curl China virus (TYLCCNV) causes a reduction in photosynthetic activity, which is part of the viral symptoms. ${\beta}C1$ is a viral factor encoded by the betasatellite DNA ($DNA{\beta}$) accompanying TYLCCNV. It is a major viral pathogenicity factor of TYLCCNV. To elucidate the effect of ${\beta}C1$ on plants' photosynthesis, we measured the relative chlorophyll (Chl) content and Chl fluorescence in TY-LCCNV-infected and ${\beta}C1$ transgenic N. benthamiana plants. The results showed that Chl content is reduced in TYLCCNV A-infected, TYLCCNV A plus $DNA{\beta}$ (TYLCCNV A + ${\beta}$)-infected and ${\beta}C1$ transgenic plants. Further, changes in Chl fluorescence parameters, such as electron transport rate, $F_v/F_m$, NPQ, and qP, revealed that photosynthetic efficiency is compromised in the aforementioned N. benthamiana plants. The presense of ${\beta}C1$ aggravated the decrease of Chl content and photosynthetic efficiency during viral infection. Additionally, the real-time quantitative PCR analysis of oxygen evolving complex genes in photosystem II, such as PsbO, PsbP, PsbQ, and PsbR, showed a significant reduction of the relative expression of these genes at the late stage of TYLCCNV A + ${\beta}$ infection and at the vegetative stage of ${\beta}C1$ transgenic N. benthamiana plants. In summary, this study revealed the pathogenicity of TYLCCNV in photosynthesis and disclosed the effect of ${\beta}C1$ in exacerbating the damage in photosynthesis efficiency by TYLCCNV infection.

• Journal of Aquaculture
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• v.19 no.4
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• pp.310-314
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• 2006

This study was carried out to investigate grazing of brackish water cyclopoid copepod Paracyclopina nana on four microalgae species (Tetraselmis suecica, Isochrysis galbana, Phaeodactylum tricornutum and Dunaliella tertiolecta) and different food concentrations raging from 5 to 55 ng chl a/ml. The grazing of P. nana was examined by the analysis of decreased number of microalgae and chlorophyll a content in rearing water and pigment content in the gut of P. nana. The maximum content of decreased chlorophyll a and gut pigment in P. nana varied with microalgae species and concentrations. It appeared at the food concentration 30 ng chl a/ml in T. suecica, 40 ng chl a/ml in I. galnaba and D. tertiolecta, and 45 ng chl a/ml in P. tricornutum, respectively. The grazing rate of a P. nana per hour also varied with different microalgae species and concentrations. The maximum grazing rate per hour of P. nana fed T. suecica with 39.3 ng chl a/ml was the highest with 0.63 ng chl a/h, but lowest with 0.52 ng chl a/h. From these results, it can be concluded that T. suecica is the best species among four microalgae species for the mass culture of P. nana and daily optimum food concentration of P. nana is $25{\sim}39$ ng chl a (approximately $10{\sim}15{\times}10^4$ cells).

• Horticultural Science & Technology
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• v.29 no.5
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• pp.433-440
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• 2011

In head lettuce seedlings, NaCl stress was treated with hydroponic culture containing 0, 50, or with 100 mM NaCl in the seedling grown up to two leaf stages. Our focus was on the effect of NaCl on fresh and dry weights, antioxidant materials, and inorganic ion level. Fresh and dry weights of head lettuce seedlings increased with the increase in salinity while the optimal growth occured at 50 mM NaCl. The chlorophyll a (Chl a), total Chl and Chl a/b ratio increased 6 days after treatment with 100 mM NaCI. However, the Chl b content decreased. Total glutathione increased only in the root of head lettuce seedlings, whereas significant increase of total arcorbate content was observed in both shoot and root after the treatment with 100 mM NaCl. In addition, the NaCl treatment resulted in the decreased level of spermidine content, and a increased spermine content. Furthermore, $Na^+$ content in shoot and root increased significantly while $K^+$, $Ca^{2+}$, and $Mg^{2+}$ content decreased. The alteration of inorganic ion level after treatment with NaCl caused the reduction of $K^+/Na^+$, $Ca^{2+}/Na^+$, and $Mg^{2+}/Na^+$ ratio with the increase of NaCl concentration. Taken together, these findings indicate that the treatment of NaCl causes the induction of oxidative stress, and results in the alteration of metabolic mechanism in head lettuce seedlings.

• Korean Journal of Environmental Agriculture
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• v.33 no.4
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• pp.344-349
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• 2014

BACKGROUND: Purpose of the study was to establish the extinction coefficients of chlorophyll a and b in N,N-dimethylformamide(DMF) and Dimethylsulfoxide(DMSO) solvents and to find out the conditions of optimal extraction temperature and time in citrus leaves. METHODS AND RESULTS: Chlorophyll a and b standards were dissolved in DMF and DMSO. Extinction coefficients of chlorophyll pigments were determined and their contents were quantified using spectrophotometer. Chlorophyllous pigments of citrus(Citrus unshiu Marc. cv. Okitsu wase) leaves were extracted at 25, 40, 60 and $80^{\circ}C$ for 4, 6, 8, 24 and 48 hours to determine the optimal extraction condition. CONCLUSION: The extinction coefficients of chlorophyll a(Chl a) and chlorophyll b(Chl b) of DMF extracts for high extinction wavelength were 663.8 and 647.2 nm. Similarly, the high extinction wavelength of DMSO extracts were 665.8 and 649.0 nm for chl a and b respectively. Chl a, Chl b and total chlorophyll content of DMF extracts were Chl a = $12.10A_{663.8}-2.74A_{647.2}$, Chl b = $21.94A_{647.2}-5.06A_{663.8}$ and total $Chl=19.193A_{647.2}+7.04A_{663.8}$. Similarly, Chl a, Chl b and total Chl of DMSO extracts were Chl a = $14.53A_{665.8}-5.40A_{649.0}$, Chl b = $26.98A_{649.0}-7.11A_{665.8}$ and total $Chl=21.58A_{649.0}+7.43A_{665.8}$. The chlorophyll extracts of DMF and DMSO were very stable in dark. High chlorophyll contents of citrus leaves were found at $80^{\circ}C$ extraction for 6 hours in DMF and at $80^{\circ}C$ extraction for 24 hours in DMSO. However, the chlorophyll content was decreased significantly after 8 hours in DMF extraction while it was remained up to 30 hours in DMSO extraction.

• Journal of Plant Biotechnology
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• v.50
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• pp.115-126
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• 2023

A hydroponics experiment was performed to study the physiological and biochemical changes in Moroccan barley (Hordeum vulgare L.) varieties cultivated under salt stress conditions. Four barley varieties were grown under exposure to three salt concentrations, including 0, 200, and 300 mM NaCl. The ANOVA for both salt stress-sensitive and resistant varieties indicated that salt treatment represented the main source of variability in all studied traits. Salt treatment significantly reduced root and shoot dry weight (RDW and SDW), relative water content (RWC), and chlorophyll content (Chl a, Chl b, and Chl T). However, increases in electrolyte leakage (EL) along with proline and total soluble sugar (TSS) contents were recorded. In addition, large variations in all measured traits were found between varieties. The 'Massine' and 'Laanaceur' varieties displayed relatively higher RDW and SDW values. The 'Amira' and 'Adrar' varieties showed lower RWC values and Chl contents than those of the controls indicating their relative sensitivity to salt stress. Principal component analysis revealed that most of the variation was captured by PC1 (72% of the total variance) which grouped samples into three categories according to salt treatment. Correlation analyses highlighted significant associations between most parameters. Positive relationships were found between RDW, SDW, RWC, Chl content, and soluble proteins contents, while all of these parameters were negatively associated with EL intensity, proline content, and TSS content. The results from this study showed that the 'Massine' and 'Laanaceur' varieties were relatively salt-tolerant. These two salt-tolerant varieties present a good genetic background for breeding of barley varieties showing high salt tolerance.

• BMB Reports
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• v.34 no.6
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• pp.496-501
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• 2001

Lincomycin, an inhibitor of plastid protein synthesis, was found to block the synthesis of apoprotein P700 with a molecular mass of 72 kDa and the assembly of the Chl a-protein of PS I. Synthesis of the polypeptides of 48, 43.5, and 32 kDa of the PS II complex is also suppressed. This process is accompanied by the disappearance of the PS Two reaction center Chl a at 683 nm, and of the PS One reaction center Chl a at 690, 696, and 705 nm on the fourth derivative of the absorption spectra at 77K. Lincomycin does not affect the synthesis of LHC subunits. It increases the content of the two main Chl forms of LHC at 648 nm (Chl b) and 676 nm (Chl a). The low-temperature fluorescence ratio F736/F685 is also increased. However, the effect of cycloheximide (an inhibitor of cytoplasmic protein synthesis) leads to the reduction of polypeptides of the light-harvesting Chl a/b-protein complex in the range of 29.5-22 kDa. Under these conditions, the relative amount of Chl b and the F736/ F685 fluorescence ratio decrease significantly. This is obviously the result of blocking the LHC I and LHC II synthesis. At the same time rifampicin and actinomycin D (inhibitors which block transcription in chloroplast and nuclear genome, respectively) inessentially affect the characteristics of these complexes.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.4
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• pp.231-237
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• 1973

Eeffect of temperature (3-day or 7-day treatment under $15^{\circ}C$, $20^{\circ}C$ and $25^{\circ}C$) on chlorophyll, nitrogen and potassium content in the second leaf blade from top of IR667 line (Suwon 213 and 214) and leading local varieties (Jinheung and Paldal) was investigated at the end of nursery, maximum tillering and flowering stage using phytotron. 1. Chlorophyll content was higher in IR667 line than in the local at $25^{\circ}C$ but reversed at $20^{\circ}C$ or $15^{\circ}C$ and chlorophyll a/b value was always high in the local. 2. Chlorophyll content per fresh weight decreased with growth and low temperature effect was greater at the end of nursery and on chlorophyll a than on chlorophyll b. 3. Chlorophyll a/b value increases with the increase of chlorophyll content and the increasing rate of chl. a/b value per chlorophyll increment tends to decrease under unfavorable condition. This decrease is greater in low temperature sensitive IR667 than local varieties. 4. According to chlorophyll retention value IR667 line was weaker at low temperature. 5. The content of total nitrogen or soluble nitrogen (methanol soluble) was decreased by low temperature. 6. Chl. (a+b)/S-N value decreased with growth and seemed not to be greatly affected by temperature and always higher in the local. 7. Potassium content (total or methanol soluble) tends to decrease at low temperature and soluble K increased with chlorophyll content. 8. High yielding ability of IR667 seems to be attributable to its high chlorophyll content at high temperature and easy leaf discoloration by low temperature or by nitrogen depression of IR667 seems to be attributable to the low Chl./S-N value.

• Journal of Environmental Science International
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• v.28 no.11
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• pp.935-942
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• 2019

Statistical analysis was carried out to identify the influence on environmental factors between paddy water chemical properties and Chl-a concentration (algae biomass index) using water samples collected in June for 3 years at rice paddy field. As a result of correlation analysis, there was a significantly negative relationships in DO(-0.366) and pH(-0.141). In contrast, significantly positive relationships were founded in COD(+0.431) and TOC(+0.422). According to the result of factor analysis, 3 factors were obtained and indicated that PC1 were Ca, K, Mg cation, PC2 were TOC, T-P, $PO{_4}^{3-}-P$ and DO, and PC3 were T-N and $NH_4{^+}-N$. As a result of linear regression analysis to develop a prediction model for chl-a concentration, the total amount of explanation was 20.6%, PC2 had the greatest influence on the increase of chl-a concentration and PC1 also showed a positive correlation but the PC3 has a negative correlation. In conclusion, carbon and phosporous content are the main factors for the increase algae blooms of rice paddy.

• Journal of Plant Biology
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• v.26 no.2
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• pp.91-99
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• 1983

The formation of chlorophyll-protein complexes (CP-complexes) during the greening of rape cotyledons (Brassica napus cv. Yongdang) was investigated by the SDS-polyacrylamide gel electrophoresis. The total chlorophyll content and Chl a/b ratio were also determined. In addition, the effects of dark treatment on the CP-complex patterns during greening have been examined with respect to their photosynthetic electron transport activity. Greening has brought about the increasein total chlorophyll content and the decrease in Chl a/b ratio, but there have been no changes in Chl a/b ratio after 24 hrs of greening. The light-harvesting chlorophyll a/b-protein complex (LHCP-complex0 was predominant during the initial greening period. Thereafter, the amout of chlorophyll a-protein complex (CP I-complex) was gradually increased. Twenty-four-hr dark treatment immediately after illumination for 6 hrs and 12 hrs resulted in the increase of the Chl a/b ration and the CP I complex, otherwise the decrease of the LHCP-complex. The LHCP/CP I ratio was gradually decreased with further greening, and appeared no change after 48 hrs illumination. The investigation of the photosynthetic electron transport activity indicated that photosystem (PS) II activity (H2Olongrightarrowp-PD*＋FeCy**) did not change, but the activity of PS I was increased suddenly due to the dark treatment. The data suggests that the increase of CP I-complex may result in that of P-700, that is, the increase of PS I activity.