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Effect of NaCl Stress on the Growth, Antioxidant Materials, and Inorganic Ion Content in Head Lettuce Seedlings  

Kim, Ju-Sung (Majors in Plant Resource Sciences and Environment, Jeju National University)
Hyun, Tae-Kyung (Division of Applied Life Science, Gyeongsang National University)
Publication Information
Horticultural Science & Technology / v.29, no.5, 2011 , pp. 433-440 More about this Journal
Abstract
In head lettuce seedlings, NaCl stress was treated with hydroponic culture containing 0, 50, or with 100 mM NaCl in the seedling grown up to two leaf stages. Our focus was on the effect of NaCl on fresh and dry weights, antioxidant materials, and inorganic ion level. Fresh and dry weights of head lettuce seedlings increased with the increase in salinity while the optimal growth occured at 50 mM NaCl. The chlorophyll a (Chl a), total Chl and Chl a/b ratio increased 6 days after treatment with 100 mM NaCI. However, the Chl b content decreased. Total glutathione increased only in the root of head lettuce seedlings, whereas significant increase of total arcorbate content was observed in both shoot and root after the treatment with 100 mM NaCl. In addition, the NaCl treatment resulted in the decreased level of spermidine content, and a increased spermine content. Furthermore, $Na^+$ content in shoot and root increased significantly while $K^+$, $Ca^{2+}$, and $Mg^{2+}$ content decreased. The alteration of inorganic ion level after treatment with NaCl caused the reduction of $K^+/Na^+$, $Ca^{2+}/Na^+$, and $Mg^{2+}/Na^+$ ratio with the increase of NaCl concentration. Taken together, these findings indicate that the treatment of NaCl causes the induction of oxidative stress, and results in the alteration of metabolic mechanism in head lettuce seedlings.
Keywords
ascorbate; glutathione; growth promotion; hydroponic culture; polyamine;
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1 Bowler, C., M. van Montagu, and D. Inze. 1992. Superoxide dismutase and stress tolerance. Annu. Rev. Plant Physiol. Mol. Biol. 43:83-116.   DOI   ScienceOn
2 Bruggemann, L.I., I.I. Pottosin, and G. Schonknecht. 1998. Cytoplasmic polyamines block the fast activating vacuolar cation channel. Plant J. 16:101-105.   DOI   ScienceOn
3 Cha, H.S., A.R. Youn, S.H. Kim, K.H. Kwon, and B.S. Kim. 2007. Evaluation of quality and analysis of hazard management at different seasons of lettuce. J. Kor. Soc. Food Sci. Nutr. 36:932-937.   DOI
4 Yamaguchi, K., Y. Takahashi, T. Berberich, A. Imai, A. Miyazaki, T. Takahashi, A. Michael, and T. Kusano. 2006. The polyamine spermine protects against high salt stress in Arabidopsis thaliana. FEBS Lett. 580:6783-6788.   DOI   ScienceOn
5 Youn, A.R., K.H. Kwon, B.S. Kim, S.H. Kim, B.S. Noh, and H.S. Cha. 2009. Effect of agrichemical during cultivation on quality and shelf-life of fresh-cut lettuce. J. Kor. Soc. Food Sci. Nutr. 38:217-224.   DOI
6 Zapata, P.J., M. Serrano, M.T. Pretel, A. Amoros, and M.A. Botella. 2003. Changes in ethylene evolution and polyamines profile of seedlings of nine cultivars of Lactuca sativa L. in response to salt stress during germination. Plant Sci. 164:557-563.   DOI   ScienceOn
7 Singh, M.P., S.K. Pandey, M. Singh, P.C. Ram and B.B. Singh. 1990. Photosynthesis, transpiration, stomatal conductance and leaf chlorophyll content in mustard genotypes grown under sodic conditions. Photosynthetica 24:623-627.
8 Simon-Sarkadi, L., G. Kocsy, and Z. Sebestyen. 2002. Effect of salt stress on free amino acid and polyamine content in cereals. Acta Biol. Szeg. 46:73-75.
9 Singh, A. and R. Prasad. 2009. Salt stress effects growth and cell wall bound enzymes in Arachis hypogaea L. seedings. Int. J. Integr. Biol. 7:117-123.
10 Singh, A.K. and R.S. Dubey. 1995. Changes in chlophyll a and b contents and activites of photosystems 1 and 2 in rice seediness incuced by NaCl. Photosynthetica 31:489-499.
11 Tang, L., S.Y. Kwon, S.S. Kwak, C.K. Sung, and H.S. Lee. 2003. Susceptibility of two potato cultivars to various environmental stresses. Kor. J. Plant Biotechnol. 30:405-410.   DOI
12 Turan, M.A., N. Turkmen, and N. Taban. 2007. Effect of NaCl on stomatal resistance and proline, chlorophyll, Na, Cl and K concentrations of Lentil plants. J. Agron. 6:378-381.   DOI   ScienceOn
13 Wang, S.Y., H.J. Jiao, and M. Faust. 1991. Changes in ascorbate, glutathione, and related enzyme activities during thidiazuroninduced bud break of apple. Physiol. Plant. 82:231-236.   DOI   ScienceOn
14 Lee, H.J. and S.E. Oh. 1994. Interrelationship between environmental stresses, reactive oxygen species and stress-ethylene. Kor. J. Ecol. 17:91-100.
15 Wang, Y. and N. Nil. 2000. Changes in chlorophyll, ribulose biphosphate carboxylase-oxygenase, glycine betaine content, photosynthesis and transpiration in Amaranthus tricolor leaves during salt stress. J. Hort. Sci. Biotechnol. 75:623-627.   DOI
16 Yamamoto, A., I.S. Shim, S. Fujihara, T. Yoneyama, and K. Usui. 2004. Effect of difference in nitrogen media on salt-stress response and contents of nitrogen compounds in rice seedlings. Soil Sci. Plant Nutr. 50:85-93.   DOI   ScienceOn
17 Lacerda, C.F., J. Cambraia, M.A. Cano, H.A. Ruiz, and J.T. Prisco. 2003. Solute accumulation and distribution during shoot and leaf development in two sorghum genotypes under salt stress. Environ. Exp. Bot. 49:107-120.   DOI   ScienceOn
18 Maiale, S., D.H. Sanchez, A. Guirado, A. Vidal, and O.A. Ruiz. 2004. Spermine accumulation under salt stress. J. Plant Physiol. 161:35-42.   DOI   ScienceOn
19 Musyimi, D.M., G.W. Netondo, and G. Ouma. 2007. Effects of salinity on gas exchange and nutrients uptake in avocados. J. Biol. Sci. 7:496-505.   DOI
20 Parida, A.K. and A.B. Das. 2005. Salt tolerance and salinity effects on plants. Ecotoxicol. Environ. Saf. 60:324-349.   DOI   ScienceOn
21 Peter, R., V. Wim, D. Frank, and D. Johan. 2004. Consumer perception and choice of minimally processed vegetable and packaged fruit. Food Qual. Prefer. 15:259-270.   DOI   ScienceOn
22 Rhee, H.C., K.H. Kang, K.B. Kweon, Y.H. Choi, and H.T. Kim. 2002. Effect of NaCl stress on the growth, photosynthetic rate and mineral uptake of tomato, red pepper, and egg plant in pot culture. J. Bio-Environment Control 11:133-138.
23 Sanchez, D.H., J.C. Cuevas, M.A. Chiesa, and O.A. Ruiz. 2005. Free spermidine and spermine content in Lotus glaber under long-term salt stress. Plant Sci. 168:541-546.   DOI   ScienceOn
24 Jamil, M., S. Rehman, K.J. Lee, J.M. Kim, H.S. Kim, and E.S. Rha. 2007. Salinity reduced growth PS2 photochemistry and chlorophyll content in radish. Sci. Agric. 64:111-118.   DOI
25 Santa-Cruz, A., M. Acosta, F. Perez-Alfocea, and M.C. Bolarin. 1997. Changes in free polyamine levels induced by salt stress in leaves of cultivated and wild tomato species. Physiol. Plant 101:341-346.   DOI   ScienceOn
26 Savoure, A., D. Thorin, M. Davey, X.J. Hua, S. Mauro, M. Van Montagu, D. Inze, and N. Verbruggen. 1999. NaCl and $CuZnSO_{4}$ treatments trigger distinct oxidative defense mechanism in Nicotiana plumbaginifolia L. Plant Cell Environ. 22:387-396.   DOI   ScienceOn
27 Jamil, M., S. Rehman, and E.S. Rha. 2007. Salinity effect on plant growth, PSII photochemistry and chlorophyll content in sugar beet (Beta vulgaris L.) and cabbage (Brassica oleracea capitata L.). Pak. J. Bot. 39:753-760.
28 Jeschke, W.D., J.S. Pate, and C.A. Atkins. 1987. Partitioning of $K^{+}$, $Na^{+}$, $Mg^{2+}$, and $Ca^{2+}$ through xylem and phloem component organs and nodulated white lupin under mild salinity. J. Plant Physiol. 128:77-93.   DOI
29 Kasugai, S. 1939. Studies on the hydroponic cultures. J. Sci. Soil Manure 13:669-822.
30 Katsuhara, M., K. Kuchitsu, K. Takeshige, and M. Tazawa. 1989. Salt stress-induced cytoplasmic acidification and vacuolar alkalinization in Nitellopsis obtuse cells. Plant Physiol. 90:1102-1107.   DOI   ScienceOn
31 Kim, B.S., D.C. Shin, S.E. Lee, G.B. Nahm, and J.W. Jeong. 1995. Freshness prolongat of crisphead lettuce by vacuum cooling and cold-chain system. Kor. J. Food Sci. Technol. 27:546-554.
32 Kim, J.S., I.S. Shim, and M.J. Kim. 2010. Physiological response of Chinese cabbage to salt stress. Kor. J. Hort. Sci. Technol. 28:343-352.
33 Chappele, E.W., M.S. Kim, and J.E. McMurtrey. 1992. Ratio analysis of reflectance spectra (RARS): An algorithm for remote estimation of the concentration of chlorophyll a, chlorophyll b, and carotenoids in soybean leaves. Remote Sens. Environ. 39:239-247.   DOI   ScienceOn
34 Kim, Y.H., I.S. Shim, K. Kobayashi, and K. Usui. 1999. Relationship between Na content or K/Na ratio in shoots and salt tolerance in several gramineous plants. J. Weed Sci. Tech. 44:293-299.   DOI   ScienceOn
35 Krishnamurthy, R. and K.A. Bhagwat. 1989. Polyamines as modulators of salt tolerance in rice cultivars. Plant Physiol. 91:500-504.   DOI   ScienceOn
36 Lacerda, C.F., J. Cambraia, M.A. Cano, and H.A. Ruiz. 2001. Plant growth and solute accumulation and distribution in two sorghum genotypes, under NaCl stress. Rev. Bras. Fisiol. Veg. 13:270-284.   DOI
37 Cheong, M.S. and D.J. Yun. 2007. Salt-stress signaling. J. Plant Biol. 50:148-155.   DOI
38 Choi, Y.J., D.C. Won, and H.D. Chung. 2003. Effects of soil EC on emergence rate, seedling growth, and physiological disorders of leafty and root vegetable crops, and diminishing effect of soil EC level by washing with water or manure adding. J. Kor. Soc. Hort. Sci. 44:575-581.
39 De Araujo, S.A.M., J.A.G. Silveira, T.D. Almeida, I.M.A. Rocha, D.L. Morais, and R.A. Viegas. 2006. Salinity tolerance of halophyte Atriplex nummularia L. grown under increasing NaCl levels. R. Bras. Eng. Agríc. Ambiental 10:848-854.   DOI
40 Efrose, R.C., E. Flemetakis, L. Sfichi, C. Stedel, E.D. Kouri, M.K. Udvardi, K. Kotzabasis, and P. Katinakis. 2008. Charaterization of spermidine and spermine synthases in Lotus japonicas: Induction and spatial organization of polyamine biosynthesis in nitrogen fixing nodules. Planta 228:37-49.   DOI   ScienceOn
41 Foyer, C.H., P. Descourvieres, and K.J. Kunert. 1994. Protection against oxygen radicals: important defense mechanism studied in transgenic plants. Plant Cell Environ. 17:507-523.   DOI   ScienceOn
42 Ashraf, M. and A. Orooj. 2006. Salt stress effects on growth, ion accumulation and seed oil concentration in an arid zone traditional medicinal plant ajwain (Trachyspermum ammi L. Sprague). J. Arid. Environ. 64:209-220.   DOI   ScienceOn
43 Gronwald, J.W., E.P. Fuerst, C.V. Eberlein, and M.A. Egli. 1987. Effect of herbicide antidotes on glutathione concentration and glutathione S-transferase activity of sorghum shoots. Pestic. Biochem. Physiol. 29:66-76.   DOI
44 Heo, E.J., H.H. Jung, and K.S. Kim. 2007. Response of Dianthus japonicus Thunb. to NaCl stress imposed at different growth stages. Hort. Environ. Biotechnol. 48:381-386.
45 Allakhverdiev, S.I., A. Sakamoto, Y. Nishiyama, M. Inada, and N. Murata. 2000. Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp. Plant Physiol. 123:1047-1056.   DOI
46 Bolu, W.H. and A. Polle. 2004. Growth and stress reactions in roots and shoots of a salt-sensitive poplar species (Populus ${\times}$ canescens). Trop. Ecol. 45:161-171.