• Animal Bioscience
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• v.35 no.6
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• pp.814-825
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• 2022

Objective: This study was conducted to identify the functional long non-coding RNAs (lncRNAs) for swine lactation by RNA-seq data of mammary gland. Methods: According to the RNA-seq data of swine mammary gland, we screened lncRNAs, performed differential expression analysis, and confirmed the functional lncRNAs for swine lactation by validation of genome wide association study (GWAS) signals, functional annotation and weighted gene co-expression network analysis (WGCNA). Results: We totally identified 286 differentially expressed (DE) lncRNAs in mammary gland at different stages from 14 days prior to (-) parturition to day 1 after (+) parturition, and the expressions of most of lncRNAs were strongly changed from day -2 to day +1. Further, the GWAS signals of sow milk ability trait were significantly enriched in DE lncRNAs. Functional annotation revealed that these DE lncRNAs were mainly involved in mammary gland and lactation developing, milk composition metabolism and colostrum function. By performing weighted WGCNA, we identified 7 out of 12 lncRNA-mRNA modules that were highly associated with the mammary gland at day -14, day -2, and day +1, in which, 35 lncRNAs and 319 mRNAs were involved. Conclusion: This study suggested that 18 lncRNAs and their 20 target genes were promising candidates for swine parturition and colostrum occurrence processes. Our research provided new insights into lncRNA profiles and their regulating mechanisms from colostrogenesis to lactogenesis in swine.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.5
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• pp.520-529
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• 2013

Needham Problem(NP) is the influential question that English historian of Chinese science Joseph Needham raised, "Why modern science had not developed in the Chinese civilisation but only in that of Europe?" Our objectives in this paper are as follows: First, we will revisit NP in the broad context of the emergence of modernity rather than treating it just as an internal problem of Chinese science. After that, the problem of modernity in Chinese medicine will be discussed from the viewpoint of NP. After NP's intellectual backgrounds are summarized, its value and implications are examined, and then Needham's own answers are presented. Afterwards, we present supplementary hypotheses, adapted from Weber, as our solution to NP in Chinese science and medicine. Needham thought that the European scientific revolution would not have been possible without the rise of modern capitalism. He also believed that Chinese bureaucratism facilitated early development of Chinese science and in turn, inhibited later radical change by interrupting the rise of capitalism. According to our hypotheses, scientific changes are related to social changes, especially to the legitimation crises, which lead to the alternations of mode of justification in sciences. The Chinese society did not go through the legitimation crises as the European society did, and therefore it failed to produce a radically different kind of justification from the traditional one. This is the reason why there was no revolution in science and medicine in China.

• Nuclear Engineering and Technology
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• v.55 no.2
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• pp.595-602
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• 2023

The EAST ICRF system operating space has been extended in power and phase control with a low-level RF system for the new double-strap antenna. Then the multi-step power and periodic phase scanning experiment were conducted in L-mode plasma, respectively. In the power scanning experiment, the stored energy, radiation power, plasma impedance and the antenna's temperature all have positive responses during the short ramp-ups of P_L;ICRF. The core ion temperature increased from 1 keV to 1.5 keV and the core heating area expanded from |Z| ≤ 5 cm to |Z| ≤ 10 cm during the injection of ICRF waves. In the phasing scanning experiment, in addition to the same conclusions as the previous relatively phasing scanning experiment, the superposition effect of the fluctuation of stored energy, radiation power and neutron yield caused by phasing change with dual antenna, resulting in the amplitude and phase shift, was also observed. The active control of RF output facilitates the precise control of plasma profiles and greatly benefits future experimental exploration.

• Animal Bioscience
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• v.34 no.6
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• pp.975-984
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• 2021

Objective: Inbreeding depression of reproduction is a major concern in the conservation of native chicken genetic resources. Here, based on the successful development of strongly inbred (Sinb) and weakly inbred (Winb) Langshan chickens, we aimed to evaluate inbreeding effects on reproductive traits and identify candidate genes involved in inbreeding depression of reproduction in Langshan chickens. Methods: A two-sample t-test was performed to estimate the differences in phenotypic values of reproductive traits between Sinb and Winb chicken groups. Three healthy chickens with reproductive trait values around the group mean values were selected from each of the groups. Differences in ovarian and hypothalamus transcriptomes between the two groups of chickens were analyzed by RNA sequencing (RNA-Seq). Results: The Sinb chicken group showed an obvious inbreeding depression in reproduction, especially for traits of age at the first egg and egg number at 300 days (p<0.01). Furthermore, 68 and 618 differentially expressed genes (DEGs) were obtained in the hypothalamus and ovary between the two chicken groups, respectively. In the hypothalamus, DEGs were mainly enriched in the pathways related to vitamin metabolism, signal transduction and development of the reproductive system, such as the riboflavin metabolism, Wnt signaling pathway, extracellular matrix-receptor interaction and focal adhesion pathways, including stimulated by retinoic acid 6, serpin family F member 1, secreted frizzled related protein 2, Wnt family member 6, and frizzled class receptor 4 genes. In the ovary, DEGs were significantly enriched in pathways associated with basic metabolism, including amino acid metabolism, oxidative phosphorylation, and glycosaminoglycan degradation. A series of key DEGs involved in folate biosynthesis (gamma-glutamyl hydrolase, guanosine triphosphate cyclohydrolase 1), oocyte meiosis and ovarian function (cytoplasmic polyadenylation element binding protein 1, structural maintenance of chromosomes 1B, and speedy/RINGO cell cycle regulator family member A), spermatogenesis and male fertility (prostaglandin D2 synthase 21 kDa), Mov10 RISC complex RNA helicase like 1, and deuterosome assembly protein 1) were identified, and these may play important roles in inbreeding depression in reproduction. Conclusion: The results improve our understanding of the regulatory mechanisms underlying inbreeding depression in chicken reproduction and provide a theoretical basis for the conservation of species resources.

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.259-262
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• 2011

In organic Chinese cabbage fields, Commercial Bacillus thuringiensis products are used widely against diamond back moth, Plutella xylostella. We conducted the study to determine the effective spray-interval of commercialized B. thuringiensis against diamond back moth on Chinese cabbages. Chinese cabbage leaves were collected 0, 1, 2, 3, 6, 10days after treatment in first trial and 0, 2, 4, 7, 9, 11days after treatment. We compared the insecticidal property of sprayed B. thuringiensis and the density of it on surface of Chinese cabbages using collected leaves. The insecticidal property maintained high until nine days after commercial B. thuringiensis products sprayed.

• Journal of the Korean Society for Library and Information Science
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• v.22
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• pp.219-257
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• 1992

Among the Chinese Characters originated in Japan, some of them are very ancient in their origin and others come to exist as different forms by being used widely in forged books in Chinese. These Characters can be divided into three groups. First, the Chinese Characters whose forms are different. Most of these are 'hoiui' (회의)character, being made by imitating the forms of the original Chinese Letters. These characters do have meaning but not pronunciation. This is one distinct feature of Chinese Characters originated in Japan. Second, the Chinese Characters whose meaning has been assigned by the Japanese people. These letters can be grouped into two. One is the letters whose meanings are entirely different from original Chinese Characters, and the other is the letters whose meanings are not known although their pronunciations are known. It can be explained that the letters with different forms are made because of the ignorance of letter's existence. Or, the letters were made on purpose in ordoer to be used in different meanings. Third, the Characters with a partial modification of original Chinese Characters. Among the Characters in three groups above, pure Japanese-made Chinese Characters are those in group one and three since those in group two are Chinese Letters whose meanings (or pronunciation) only are Japanese. As a results of detailed investigation of pure Japanese-made Chinese Character in JIS X 0208-1990, the followings are discovered: 1. Pure Japanese-made Chinese Characters are 147 in numbers. 2. The Characters which were originally Chinese but now considered to be Japanese-made are 5 in numbers. Among these letters, 39 Characters are not listed in TaeHanHwaSaJon(Whose fame is well known as the authoritative dictionary of Chinese Characters), 47 Characters are not found in the dictionaries of Chinese Characters compiled in Korea. 3. 14 Characters seem to be Japanese-made Chinese Characters although it cannot be said so with accuracy because of various meanings found in several dictionaries of Chinese Characrters.

• Parasites, Hosts and Diseases
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• v.51 no.5
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• pp.511-517
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• 2013

Species identification using DNA sequences is the basis for DNA taxonomy. In this study, we sequenced the ribosomal large-subunit RNA gene sequences (3,037-3,061 bp) in length of 13 Chinese Theileria stocks that were infective to cattle and sheep. The complete 28S rRNA gene is relatively difficult to amplify and its conserved region is not important for phylogenetic study. Therefore, we selected the D2-D3 region from the complete 28S rRNA sequences for phylogenetic analysis. Our analyses of 28S rRNA gene sequences showed that the 28S rRNA was useful as a phylogenetic marker for analyzing the relationships among Theileria spp. in ruminants. In addition, the D2-D3 region was a short segment that could be used instead of the whole 28S rRNA sequence during the phylogenetic analysis of Theileria, and it may be an ideal DNA barcode.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.7
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• pp.903-909
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• 2004

The variability of mtDNA hypervariable segment I (HVS I) sequences was investigated in a total of 48 birds belonging to 12 Chinese native chicken breeds. Sixteen haplotypes were identified from 35 polymorphic nucleotide sites which accounted for 6.4% of a sequenced 544 bp fragment. Diversity analysis of the haplotypes showed that Tibetan, Langshan and Henan cockfight chicken had only one haplotype, while ancient haplotypes existed in Taihe silky and Chahua chicken. Phylogenetic analysis of the haplotypes suggested that Chinese native chicken breeds shared 5 maternal lineages and some breeds would share the same maternal lineage, regardless of their external features and ecological types. Both divergent and phylogenetic analysis of the haplotypes indicated the close genetic relationships between the Chinese native chicken breeds and G. g. gallus and G. g. spadiceus from different areas, which implied that G. g. gallus and G. g. spadiceus were the original ancestors of the Chinese native chicken breeds.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.5
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• pp.556-563
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• 2015

Chinese cabbage is one of the most important vegetables in Korea and a target crop for market stabilization as well. Remote sensing has long been used as a tool to extract plant growth, cultivated area and yield information for many crops, but little research has been conducted on Chinese cabbage. This study refers to the derivation of simple Chinese cabbage growth prediction equation by using RapidEye derived vegetation index. Daesan-myeon area in Gochang-gun, Jeollabuk-do, Korea is one of main producing district of Chinese cabbage. RapidEye multi-spectral imagery was taken on the Daesan-myeon five times from early September to late October during the Chinese cabbage growing season. Meanwhile, field reflectance spectra and five plant growth parameters, including plant height (P.H.), plant diameter (P.D.), leaf height (L.H.), leaf length (L.L.) and leaf number (L.N.), were measured for about 20 plants (ten plants per plot) for each ground survey. The normalized difference vegetation index (NDVI) for each of the 20 plants was measured using an active plant growth sensor (Crop $Circle^{TM}$) at the same time. The results of correlation analysis between the vegetation indices and Chinese cabbage growth data showed that NDVI was the most suited for monitoring the L.H. (r=0.958~0.978), L.L. (r=0.950~0.971), P.H. (r=0.887~0.982), P.D. (r=0.855~0.932) and L.N. (r=0.718~0.968). Retrieval equations were developed for estimating Chinese cabbage growth parameters using NDVI. These results obtained using the NDVI is effective provided a basis for establishing retrieval algorithm for the biophysical properties of Chinese cabbage. These results will also be useful in determining the RapidEye multi-spectral imagery necessary to estimate parameters of Chinese cabbage.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.11
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• pp.1405-1411
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• 2010

Calpastatin (CAST), an endogenous inhibitor of the calpains, plays an important role in post-mortem tenderization of meat. The objectives of this study were to investigate single nucleotide polymorphisms (SNPs) in the bovine CAST gene and association with carcass and meat quality traits. A total of 212 cattle from commercial herds were tested in this study including 2 pure introduced breeds, 4 cross populations, and 3 pure Chinese native breeds. Five SNPs were identified at position 2959 (A/G), 2870 (G/A), 3088 (C/T), 3029 (G/A) and 2857 (C/T) in the CAST gene (GenBank Accession No. AF159246). Allele frequencies of SNP2959 and SNP2870 were 0.701 (A) and 0.462 (A), respectively. A general linear model was used to evaluate the associations between the two markers and 7 traits. The results showed that both SNP2959 and SNP2870 were significantly (p<0.01) associated with the Warner-Bratzler shear force (WBSF), while they had no significant association with the other 6 traits in the whole population. However, in Chinese native pure breeds, only SNP2870 had significant association with WBSF (p<0.05). The simultaneous analysis of two-marker genotype effects indicated animals containing the A/G haplotype (A for SNP2959 and G for SNP2870) tended to have lower shear force than those containing the G/A haplotype, and, especially, animals homozygous for the A/G haplotype had approximately 2 kg lower shear force than those homozygous for the G/A haplotype (p<0.01). These results suggested that both markers may be effective for the marker-assisted selection of meat quality traits in Chinese commercial herds, especially SNP2870 which can be used for Chinese native cattle.