• Title/Summary/Keyword: Chimeric thallus

Search Result 1, Processing Time 0.016 seconds

Cross-breeding of Neopyropia spp. (Bangiales, Rhodophyta) Using CAPS (Cleaved Amplified Polymorphic Sequence) Markers (CAPS (Cleaved Amplified Polymorphic Sequence) 마커를 적용한 김 교잡육종 기술 개발)

  • Eun-Jeong Park
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.56 no.1
    • /
    • pp.124-132
    • /
    • 2023
  • This study aimed to cross between Korean and Japanese pure lines of Neopyropia strains to establish cross breeding technology and identify a superior variety that harbors the strength of both parents. Four crossing combinations were tried using three methods, resulting in 1,476 single conchocelis colonies. The three co-dominant Cleaved Amplified Polymorphic Sequence (CAPS) markers (EF-1α/Mse I, TOP2/Mse I, car A/ApaL I) were used to distinguish heterozygotic sporophytes and their maternal lines obtained from the inter and intraspecific cross-fertilization within the wild type of Neopyropia strains. Of the 1,476 colonies, 26.9% (218) were heterozygotes obtained from the nuclear CAPS markers. Their maternal line was clearly confirmed using organelle CAPS marker and chimeric thallus was obtained from crossing experiment of Japanese N. yezoensis (♀) and Korean N. yezoensis (♂). The use of CAPS markers improved the efficiency of crossbreeding by quickly screening heterozygotes and maternal lines in the conchocelis phase, which otherwise required pigmentation mutants as genetic markers.