• Archives of Pharmacal Research
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• v.20 no.5
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• pp.459-464
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• 1997

The human liver cDNA clone UDPGTh2, encoding a liver UDP-glucuronosyltransferase (UDPGT) was isolated from a .gamma. gt 11 cDNA library by hybridization to mouse transferase cDNA clone, UDPGTm1. UDPGTh2 encoded a 529 amino acid protein with an amino terminus membrane-insertion signal peptide and a carboxyl terminus membrane-spanning region. There were three potential asparagine-linked glycosylation sites at residues 67, 68, and 315. In order to obtain UDPGTh2 protein encoded from cloned human liver UDP-glucuronosyltransferase cDNA, the clone was inserted into the pSVL vector (pUDPGTh2) and expressed in COS 1 cells. The presence of a transferase with Mr-52,000 in transfected cells cultured in the presence of $[^{35}S]$ methionine was shown by immunocomplexed products with goat antimouse transferase IgG and protein A-Sepharose and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. The expressed UDPGT was a glycoprotein as indicated by electrophoretic mobility shift in Mr-3,000-4,000 when expressed in the presence of tunicamycin. The extent of glycosylation was difficult to assess, although one could assume that glycosyl structures incorporated at the level of endoplasmic reticulum were always the core oligosaccharides. Thus, it is likely that at least two moieties inserted can account for the shift of Mr-3,000-4,000. This study demonstrates the cDNA and deduced amino acid sequence of human liver UDP-glucuronosyltransferase cDNA, UDPGTh2.

• Journal of Korean Clinical Health Science
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• v.2 no.3
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• pp.167-175
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• 2014

Purpose. The purpose of this study was to analyze the trend of the narrative therapy research on 42 studies, the master's and doctor's dissertations in Korea and to propose a direction of the narrative therapy study. Methods. The material used for this study were 42 studies collected from National Digital Library database. The analytic frame of this study is on the year of study, research design, research subjects, key concept, major of researcher and narrative therapy program. Results. Regarding research design, quasi-experimental study was the most used and survey study, program development research in this order. As a research subject, adolescents were the most used and child, aged, in this order. As the key concept, self-esteem was the most used and there were aggression, depression, self-efficacy, etc. As the major of researcher, counseling were the most used and theology, pedagogy, in this order. Conclusions. As a result on the research above, following recommendation can be made. Narrative therapy program should be develop the verify on effect of variables. Narrative therapy study needs to expanded to various subjects. In education field, we should be develop the various methods to apply narrative therapy.

• Perspectives in Nursing Science
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• v.17 no.2
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• pp.80-89
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• 2020

Purpose: The aim of this study is to review literature on anger management intervention programs for parents published over the last 10 years and to extract the key elements of the interventions through an integrative review. Methods: This research was carried out in stages following Whittemore and Knafl's integrative literature methodology. Key words in Korean and English were used to search the PubMed, MEDLINE, EMbase, CINAHL, RISS, KISS and National Assembly Library databases. Several intervention factors were extracted from the selected papers on the basis of the framework which was helpful to identify the intervention patterns and were classified into meaningful themes. Results: The extracted intervention factors from the final nine studies classified into four themes: 1) Modifying irrational beliefs through cognitive approaches, 2) Empowering parenting competencies through learning a parent's role, 3) Utilizing emotion management skills, and 4) Parent-child relationship improvement training based on self-reflection. Conclusion: Four main themes were drawn from the key components of the various interventions. These findings should be considered in practice, and further intervention development studies for parents using these findings should be conducted.

• Journal of Families and Better Life
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• v.27 no.5
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• pp.43-57
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• 2009

This study analyzes the tendency for studies of children's manners in Korea. This research provides basic information for future research on the study of children's manners. I reviewed previous research during Mar.10~Apr. 30., 2009, and published articles that used terms such as 'ye-jul', 'yua ye-jul', 'jun-tong ye-jul', and 'yua-dare kyo yuk' as key-words provided by the National Assembly Library and Academic Information Center, and the terms were used for contents analysis. In the past, parents usually taught manners to their children at home, but now, most children learn etiquette at kindergarten and day care centers. A greater portion of the studies dealing with children's manners has been published since 2001. The experimental method was used for most of this research. Based on the results, I have made suggestion regarding the direction of future studies of children's manners. Research should investigate the value of children's manners and philosophy in future research. Based on the personal characteristics of each child and their understanding of the ecological environment, studies of children's manners require an interdisciplinary research approach or ecological research approach. These studies should continue to research in cooperation with kindergartens and day care centers. In addition, further research should focus on an effective teaching method or instructional media research of children's manners.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2004.11a
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• pp.191-202
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• 2004

한국화합물은행(KCB)에서 보유중인 12만 개의 화합물을 주요 골격에 따라 분류하고, 4 가지 protease 작용점에 대한 활성도와 골격사이의 관계를 조사하였다. 화합물들은 합성기관의 합성 목적과 주요 고리골격 등을 고려하여 분류되었으며, 이를 이용하여 scaffold 분류를 위한 분류 계통도를 작성하였다. 화합물들은 이 계통도에 따라 7 가지의 race, 168 tribe, 493 parent, 439 child, 325 grandchild 등 1,087개의 scaffold로 분류되었으며, 각 race 및 scaffold 별 골격의 개수는 고르게 분포되었다. 골격별 분류 시스템을 이용하여 4 가지의 protease에 대한 활성도와 골격 간의 상관관계를 조사한 결과, Protease C에 대하여 몇 가지 골격이 활성이 뛰어남을 보였다.

• Korean Journal of Childcare and Education
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• v.12 no.4
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• pp.41-69
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• 2016

The purpose of this study is to promote the use of cultural facilities of North Korean refugee mothers with young children by finding out their past experience of cultural facility use. The subjects of this study were 183 North Korean refugee mothers with young children and a total of four research participants. A survey and in-depth interviews were used to draw reliable research results. The research results are as follows: First, as to uses of cultural facilities, North Korean refugee mothers with young children got information about cultural facilities through the Internet community such as Internet cafes and blogs. Their considerations for using cultural facilities were diversity of activity programs and subdivision of performances and arts activities. Over 50% of mothers responded that cultural facilities were needed to enhance the creativity of their children. Second, concerning difficulties of cultural facility use, the most difficulty was high costs, and the second most difficulty was absence of cultural facilities nearby their residence. Third, as to the needs of cultural facility use, a children's library was needed the most for children's development. They responded that the most necessary policy for cultural facility use was to increase cultural vouchers for low-income households and to expand recipients of these vouchers.

• Journal of Dental Anesthesia and Pain Medicine
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• v.20 no.3
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• pp.107-118
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• 2020

Chloral hydrate is the oldest and most common sedative drug used in moderate sedation for pediatric dental patients. Hence, the purpose of this article is to review the safety and possible adverse events of this drug when used for pediatric dental treatment. A bibliographic search in PubMed, MEDLINE, Cochrane Library and KMbase, KISS, DBpia, KoreaMed, and RISS databases was performed. Using the keywords "dental sedation," "chloral hydrate," and "children or adolescent," 512 scientific articles were found. Subsequently, 183 studies were individually assessed for their suitability for inclusion in this literature review. Altogether, 24 studies were selected. They included 12 cases of death before, during, or after chloral hydrate sedation for dental treatment, majorly due to dosing error and use of multiple sedatives. Additionally, intraoperative adverse events were mostly respiratory problems such as hypoxia and apnea, but most events were temporary. After treatment, prolonged sedation, including excessive sleep and less activity were the most common postoperative adverse events, and even death cases were reported. Despite the wide acceptance of chloral hydrate as a sedative-hypnotic agent, the risk of adverse events and adequate dose should be of great concern when using it for pediatric dental sedation.

• Journal of Dental Anesthesia and Pain Medicine
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• v.22 no.6
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• pp.395-404
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• 2022

Hydroxyzine is one of the most popular oral sedatives used in pediatric dentistry. This study aimed to investigate the safety and possible side effects of sedation using hydroxyzine in pediatric dentistry. "Hydroxyzine," "Dental sedation," "Child," and "Safety" and their associated synonyms were searched using the Cochrane Library, Embase, PubMed, KISS, KMBASE, and KoreaMed databases. Academic information and portals of DBpia and RISS were also perused. Altogether, 340 papers were found, among which a total of 24 papers were selected according to the detailed criteria. Nine studies used hydroxyzine as monotherapy, and 10 studies compared its safety when hydroxyzine used as multitherapy. In addition, seven studies employed a drug regimen wherein hydroxyzine was one of the components. All these studies revealed that the adverse events specific to hydroxyzine usage were drowsiness and dryness of the mouth, and that there were respiratory complications due to a synergistic reaction of hydroxyzine. Although classified as a histamine blocker, hydroxyzine with its sedative, antiemetic, anticonvulsant, and anticholinergic properties is an oral sedative available without serious adverse events, If the proper dosage of the drug is used and its synergistic effects with other drugs are ascertained in the route of administration.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.26 no.4
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• pp.65-82
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• 2015

This study tries to discuss the problems of reading education based on the theory of multiple intelligences and after reading activity. For the theory of multiple intelligences, Dr. Howard Gardner asserted eight types of multiple intelligences under the presupposition the anyone had infinite ability. He analyzed and applied reading education by types of multiple intelligences and proposed a new program for the reading education aiming to evenly promote children's various intelligences and heighten their creativities. The purpose of the new program is to have children throw away the idea that they should perfect their resultants in reading education time, develop their own strong intelligences through various experiences and processes and complement their lacking abilities. To prove the validity of the program I made FGI (Focus Group Interview) to the sample group consisting of child reading education experts. Analyzing the interview showed that the general idea of the group corresponded to the researcher's expectation effect. I see that reading education has one-dimensionally developed significance if it enables all the children of the present and the future not to have the oppressive feeling that they should produce masterpieces in reading education but to take pleasure in the activities and improve their own abilities.

• Journal of Aquaculture
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• v.17 no.1
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• pp.1-7
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• 2004

A rainbow trout uncoupling protein 3 (UCP3) cDNA clone, encoding a 310 amino acid protein, was cloned and sequenced from a liver cDNA library. Two different splice variants designated UCP3-vl and UCP3-v2, were identified through liver cDNA library screening using rainbow trout UCP3 cDNA clone as a probe. UCP3-vl has 3 insertions in the UCP3 cDNA: the first insertion (133 bp), the second (141 bp), and the third (370 bp) were located 126 bp, 334 bp and 532 bp downstream from the start codon, respectively. UCP3-v2 contained a single insertion, identical in sequence and location to the second insertion of UCP3-vl. UCP3, a mitochondrial protein, functions to modulate the efficiency of oxidative phosphorylation. UCP3 has been detected from heart, testis, spinal cord, eye, retina, colon, muscle, brown adipose tissue and white adipose tissue in mammalian animals. Human and rodent UCP3s are highly expressed in skeletal muscle and brown adipose tissue, while they show weak expression of UCP3 in heart and white adipose tissue. In contrast to mammalian studies, RT-PCR and Southern blot analysis of the rainbow trout demonstrated that UCP3 is strongly expressed in liver and heart. UCP3, UCP3-vl, and UCP3-v2 all contain an Ava III short interspersed element (SINE), located in the 3'untraslated region (UTR). PCR using primers from the Ava III SINE and the UCP3 3'UTR region indicates that the UCP3 cDNA is structurally conserved among salmonids and that these primers may be useful for salmonid species genotyping.