• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.503-504
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• 2001

Characteristics of $CO_2$ fixation by Chlorella sp. HA -1 against NO and $SO_2$ were investigated. Culture pH in nitric oxide gas remained stable indicating that nitric oxide was not likely to be a problem for growth, while $SO_2$ could inhibit the cell growth because of pH drop. Chiarella sp. HA -1 containing 10% $CO_2$ from stack gas can be tolerant to 100 ppm nitric oxide and 100 ppm sulfuric oxide through pH control.

• KSBB Journal
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• v.8 no.5
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• pp.438-445
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• 1993

Raw cow manure was treated by a 4-step integrated system with phase separation anaerobic digestion and algal culture. When the first methane fermentation was performed by the effluent from the acid fermenter with retention time of 4 days, the elrerage blogas production rate was 977m1/1 culture/day Gas productivity compared to conventional single-stage anaerobic digestion increased up to 31.4%. As the 2nd methane fermenter was fed by the effluent from the first methane fermenter with 4 days of retention time, average amount of 428m1/1 culture/day of biogas was produced. The reduction rate of COD in the effluent from the acid fermenter, the 1st and the 2nd methane fermenter were 71.8%, 42.6% and 24.0% respectively. Finally, we examined algal treatment process for the effluent from the 2nd methane fermenter. A semi-continuous culture of Chlorella sp. PSH3 was conducted by feeding the effluent with retention time of 10days. In this process, the production rate of algal biomass and COD reduction rate were averaged 1.8g/1 culture/day(2.8$\times$106 cells/ml) and 73%, respectively. Through the 4-setp treatments, the total chemical oxygen demand was reduced from 51,300ppm to 85ppm. Therefore, the reduction rate of total chemical oxygen demand reached about 99.8%. The results indicate that the integrated system could be applicable for treatment of organic wastes, concurrently producing biogas and algal biomass.

• Molecular & Cellular Toxicology
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• v.5 no.1
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• pp.88-92
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• 2009

The effect of Chlorella vulgaris (CV) on the urinary excretion of di-ortho PCB congeners (PCB-138, -153) was investigated. Sprague-Dawley rats (6-weeks-old, n=10 rats/group) were randomly divided into one control (0CV) or 2% CV (2CV) or 5% CV (5CV) or 10% CV (10CV) groups, respectively. Composition of normal and chlorella meal-based diet were made up of 30% casein, 15% cornstarch, 50% sucrose, 5% cellulose, 5% coconut oil, 3.5% mineral mixture, 1 % vitamin mixture. All rats had free access to water and diet for 4 weeks. A significant increase in both PCB 138 and 153 in urinary level was detected in CV fed groups, 540% and 167% for 2CV, 155% and 89% for 5CV, 114% and 144% for 10CV group, respectively, when compared with their controls. These findings suggest that CV may have potential to eliminate body burden levels of dioxin-like PCB compounds.

• Nutrition Research and Practice
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• v.2 no.4
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• pp.204-210
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• 2008

This study was performed to investigate effects of Chiarella vulgaris on lipid metabolism in rats fed high fat diet. Sixty 6-week-old male Wistar rats were divided into two groups; normal diet group and high fat diet group, then the rats in each group were further divided into three subgroups and fed 0%, 5% and 10% (w/w) chlorella-containing diets, respectively, and raised for 9 weeks, Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and total protein and albumin concentration were not different among groups. Serum total lipids and liver TG concentration were significantly lower in 5% and 10% chlorella groups than 0% chlorella group in high fat diet groups (p<0.05). Serum TG, serum total cholesterol, liver total lipid and liver total cholesterol concentrations were significantly lower in 10% chlorella groups than 0% chlorella group in high fat diet groups (p<0.05). Fecal total lipid, TG and total cholesterol excretions were significantly higher in 5% and 10% chlorella groups than 0% chlorella groups in normal diet and high fat diet groups, respectively (p<0.05). These results suggest that Chlorella vulgaris is effective for prevention of dyslipidemia which may be due to the modulation of lipid metabolism and increased fecal excretion of lipid.