• Biomolecules & Therapeutics
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• 제18권4호
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• pp.433-441
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• 2010

Chenopodium album Linne (CAL) is a fast-growing weedy annual plant. The leaves and young shoots may be eaten as a leafy vegetable. In oriental medicine, CAL has been used for treatment of skin disease, fever, stomach ache, toothache, and paralysis. After a preliminary screening of CAL ethanol extract and its fractions obtained from CAL leaves for anti-gastritic and anti-Helicobacter pylori (H. pylori) activity, the butanol (BuOH) fraction was found to have the most significant effect. We also examined antioxidative properties of the total CAL extract and its fractions, and also betaine as an ingredient of the BuOH fraction. To investigate the antioxidant effects of CAL on gastritis, the reducing power, free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH), and lipid peroxidation effects were determined. Additionally, the BuOH fraction reduced cell viability in a concentration dependent manner in human gastric cancer cell lines. The results of this study revealed that CAL has excellent antioxidant activity, and may be useful in treating gastritis and gastric cancer.

• Biomolecules & Therapeutics
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• 제19권4호
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• pp.487-492
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• 2011

In our previous study, we investigated Chenopodium album Linne (CAL) ethanol extract and its fractions on anti-gastritic actions using the HCl/ethanol and indomethacin induced gastric lesion model and Helicobacter pylori (H. pylori). Based on the results, butanol fraction was most effective among fractions obtained from CAL. This study aims to elucidate the mechanisms of butanol fraction, and betaine as a constituent of the butanol fraction, on gastritis and anti-gastric cancer cell growth. First, we examined antioxidant properties using hydrogen peroxide and superoxide radical, and we found that butanol fraction and betaine may be good antioxidants. Second, cytotoxicity was assessed by measuring cell viability and 4,6-diamidino-2-phenylinodole dihydrochloride (DAPI) staining of human gastric cancer cells (AGS cells). We also examined the relationship between the cytotoxicity and intracellular $Ca^{2+}$ signaling mechanism. The butanol fraction demonstrated cell viability 71.49% at the concentration of 100 ${\mu}g/ml$ and increased intracellular $Ca^{2+}$ concentration in a dose dependent manner. Finally, we observed the mucus content as a defensive factor and gastric secretion as an aggressive factor, and found that the mucus content noticeably increased when treated with butanol fraction and betaine and gastric secretion decreased when treated with betaine in vivo study. From these results, we suggest that CAL butanol fraction and betaine may have protective effects on gastritis.

• 환경위생공학
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• 제5권2호
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• pp.105-116
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• 1990

승봉도는 인천항으로 부터 약100여km 떨어진 곳에 위치한 소도로서 1981년 6월 30일부터 7월 1일까지 2일간, 1981년 8월 20일부터 21일까지 2일간 조사에 의해서 밝혀진 결과는 다음과 같다. 본 도서의 소생식물은 총 193종(70과, 151속, 170종, 22변종, 1품종)으로 곰솔(Pinus thunbergii)이 우점종으로 밝혀졌으며 자연도가 높은 것을 볼 수 있었다. 양치식물 계수(Pte-Q)는 0.95로 한반도전체(1.7)보다 낮은 값을 나타내었다. 또한 곰솔 임상하에는 삽주, 억새 및 진달래가 주로 분포되어 있고 명아주, 강아지풀 및 비름 등의 분포가 빈약한 것은 곰솔 잎에서 분필되는 어떠한 화학물질에 의한 allelopathy현상으로 생각된다. 타도서에 비하여 화작지와 초지의 면적이 적기 때문인것으로 생각되며, 우점종인 곰솔림하의 식물의 생장이 빈약한것은 곰솔 림하의 상대 조도가 낮은데 그 원인이 있다고 생각된다. 해양의 식물은 총 11종으로 비교적 종수가 다양하였다.