• ALGAE
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• 제28권3호
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• pp.289-296
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• 2013

For the quantitative detection of algal toxin, microcystin, a chemiluminescence immunochromatographic assay method was developed. The developed system consists of four parts, chemiluminescence assay strip (nitrocellulose membrane), horse radish peroxidase labeled microcystin monoclonal antibodies, chemiluminescence substrate (luminol and hydrogen peroxide), and luminometer. The performance of the chemiluminescence immunochromatographic assay system was compared with high performance liquid chromatography (HPLC) detection. The detection limit of chemiluminescence immunochromatographic assay system is several orders of magnitude lower than with HPLC. The chemiluminescence immunochromatography and HPLC results correlated very well with the correlation coefficient ($r^2$) of 0.979.

• Current Research on Agriculture and Life Sciences
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• 제24권
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• pp.9-16
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• 2006

Ultraweak photons could be emitted from the chemical reaction without any electromagnetic radient. These chemiluminescence could be detected by highly sensible photomultiplier recently. We will introduce the principal of chemiluminescence generation reaction, and the application studies in the determination of food-borne microorganisms in field. The counts of surface contaminated microorganism in mackeral and chicken for cold storage determined by ATP-chemiluminescence method had good correlation with the counts by standard agar plate method. However, there was little correlation between 2 methods in ginseng powder whose microbial counts were lower than 10,000 CFU.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2003년도 International Symposium on Clean Environment
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• pp.109-112
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• 2003

A method to determine As(V) ions in aqueous solution by chemiluminescence method has been studied using a stopped flow system. The method is based on the increased chemiluminescence intensity with the addition of As(V) ion to a solution of lucigenin and hydrogen peroxide. The effects of KOH concentration, $H_2O_2$ concentration and flow rate of reagents on the chemiluminescence intensity have been investigated. The calibration curve for As(V) was linear over the range from $1.0{\times}l0^{-6}$M to $1.0{\times}l0^{-4}$M, the coefficient of correlation was 0.997 and the detection limit was $3.3{\times}l0^{-7}$M under the optimal experimental conditions.

• 센서학회지
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• 제15권5호
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• pp.317-322
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• 2006

A method to determine quinine in aqueous solution by chemiluminescence method using a stopped flow system has been studied. The method is based on the increased chemiluminescence intensity with the addition of quinine to a solution of lucigenin and hydrogen peroxide. The effects of KOH concentration, flow rate of reagents, $H_{2}O_{2}$ concentration used for the masking of quinine on the chemiluminescence intensity have been investigated. The calibration curve for quinine was linear over the range from $1.0{\times}10^{-7}$ M to $1.0{\times}10^{-3}$ M, coefficient of correlation was 0.993 and the detection limit was $3.0{\times}10^{-8}$ M under the optimal experimental conditions of 1.0 M, 1.5 M, 3.0 mL/min for the concentration of $H_{2}O_{2}$, KOH and flow rate of reagents, respectively.

• 한국식품영양과학회지
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• 제25권2호
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• pp.193-198
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• 1996

담즙산과 결합을 하여 콜레스테롤 농도에 영향을 주는 것으로 보고된 대두 단백질의 생체내 효소에 의한 미분해성 분획물 개량 메주로부터 분리하였다. 개량 메주로부터 pH 6.8 과 pH 4.5의 분획물들을 분리하여, 초음파 처리와 투석을 행한 후 Sephadex G-75 column chromatography를 실시하여 부분 정제하였다. 부분 정제된 분획물들을 chemiluminescence 방법에 의해 in vitro에서 검색을 한 결과, pH 4.5 분획물중의 하나가 담즙산과 강하게 결합하는 것으로 밝혀졌다. 또한 대두 기원의 pepsin 비분해성 분획물 또한 pH 4.5 분획물의 chemiluminescence 결과와 일치하였다. 따라서 본 연구에서 고안한 chemiluminescence 법은 in vitro에서 담즙산 결합 활성도 측정 및 콜레스테롤 대사에 관한 연구의 기본적인 연구 수단을 평가되며 따라서 본 연구 결과는 이 분야의 연구에 기초 자료로 제공될 수 있는 가능성을 제시하였다.

• Bulletin of the Korean Chemical Society
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• 제32권1호
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• pp.149-152
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• 2011

A new chemiluminescence immunochromatographic analysis system with high sensitivity and high reproducibility was developed for the determination of microcystins (MCs) in water. Horse radish peroxidase (HRP) labeled microcystin monoclonal antibody was used for the sensitive chemiluminescence detection. The chemiluminescence immunochromatographic analysis system was composed of microcystin LR (MCLR)-monoclonal antibody (mAb)-Horse Radish Peroxidase (HRP) conjugate, MCLR-BSA conjugate, luminol, hydrogen peroxide mixture solution, an immunochromatographic assay strip and luminometer. To detect the concentration of microcystins in water, we utilized one spot analysis of the strip instead of flow type analysis. We could detect the microcystins in water at a concentration as low as 9.45 pg/mL with the chemiluminescence (CL) detection.

• 한국안전학회지
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• 제9권4호
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• pp.69-76
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• 1994

Low density polyethylene is widely used as an insulating material In electric power cables. It is most important to know the beginning of its aging by partial discharge and UV-ray. That Is reason which we investigate chemiluminescence of the LDPE treated by partial discharge, UV-ray and corona discharge. We find that it takes one hour on spectra of IR but 0.17 minutes on chemiluminescence in the variation of chemical by partial discharge. Also we knew that Lithium carbonate-added(1phr) LDPE offered obvious suppressing effect on aging by partial discharge through the chemiluminescence. Therefore, the chemiluminescence is a highly sensitive and effective measurement method to appreciate characteristics of the treated surface and the oxidation reaction.

• 대한화학회지
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• 제45권3호
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• pp.223-229
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• 2001

흐름주입 장치를 이용하여 화학발광법에 의한 전혈 중의 당을 정량하는 방법에 대하여 연구하였다. 당의 효소반응에서 생성되는 과산화수소에 의하여 424nm에서 발생하는 luminol의 화학발광 세기의 차이를 정량에 이용하였다. 효소반응기는 glucose oxidase를 aminopropyl glass bead에 혼입하여 만들었으며, 흐름셀에서 발생하는 화학발광의 세기는 광섬유 다발을 이용하여 측정하였다. 최적 실험조건을 구하기 위하여 화학발광 시약 및 효소반응기의 pH, 흐름속도 및 온도가 화학발광세기에 미치는 영향을 조사하였다. 최적실험조건에서 구한 검정 곡선은 $1.0{\times}10^{-1}$~ 7.0mM에서 직선성이 성립하였으며, 검출한계는 $6.0{\times}10^{-2}$mM이었다. 본 방법을 전혈 중의 당 정량에 적용하였으며, 그 결과를 기존의 분석법에서 구한 결과와 비교하였다. 또한, 회수율 측정을 통하여 본 방법의 신뢰성을 검증하였다.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2003년도 International Symposium on Clean Environment
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• pp.103-108
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• 2003

A method to determine chromium(III) ion in aqueous solution by chemiluminescence method using a lucigenin entrapped silica sol-gel film has been studied. An optical probe for chromium(III) ion has been prepared by entrapping lucigenin into silica sol-gel film coated on a glass support by dip coating. The chromium(III) optical sensor is based on the catalytic effect of chromium(IIII) ion on the reaction between lucigenin and hydrogen peroxide in basic solutions. The effects of Nafion, DMF and Triton X-100 were investigated to find the optimum condition to minimize cracking and leaching from the probe. The effects of pH and concentrations of lucigenin and hydrogen peroxide on the chemiluminescence intensity were investigated. The chemiluminescence intensity was increased linearly with increasing chromium(III) concentration from $2.5{\times}10^{-4}$M to $8.0{\times}10^{-7}$M and the detection limit was $4.0{\times}10^{-7}$M.

• 한국연소학회지
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• 제20권1호
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• pp.24-31
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• 2015

Flame structure, which contains a useful information for studying combustion instability of the flame, is often quantitatively visualized with PLIF (planar laser-induced fluorescence) and/or chemiluminescence images. The latter, a line-integral of a flame property, needs to be preprocessed before being inverted, mainly due to its inherent noise and the axisymmetry assumption of the inversion. A preprocessing scheme utilizing multi-division of ROI (region of interest) of the chemiluminescence image is proposed. Its feasibility has been tested with OH PLIF and $OH^*$ chemiluminescence images of SNG (synthetic natural gas) swirl-stabilized flames taken from a model gas turbine combustor. It turns out that the multi-division technique outperforms two conventional ones: those are, one without preprocessing and the other with uni-division preprocessing, reconstructing the SNG flame structure much better than its two counterparts, when compared with the corresponding OH PLIF images. It is also found that the Canny edge detection algorithm used for detecting edges in the multi-division method works better than the Sobel algorithm does.