• 대한기계학회논문집B
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• 제36권2호
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• pp.189-196
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• 2012

현 운행중인 중수로의 안전장치인 감속재는 원전사고시 최종 열침원의 역할을 감당한다. 감속재 연구 수행을 위해 CANDU6 의 축소화 모델인 HUKINS 는 최대출력 10kW 로, 칼란드리아 직경은 원모델의 1/8 에 해당하는 0.95m 이며 축방향 길이가 38.4mm 의 열원 88 개가 삽입되어 있다. HUKINS 내 감속재 유동패턴의 발생 여부를 판단하고자 화학처리기법을 활용하였고 그 결과 출력파워 약 7.7kW 에서 각입력유량을 4,7,11L/min 으로 유입시 감속재의 유동패턴이 부력기조유동, 혼합양상유동, 모멘텀 기조유동의 양상을 나타났다. 3 가지 유동패턴에 대해 육면체 격자를 기본으로 구성된 약 190 만개의 격자수 내에서 난류모델 $k{\varepsilon}$의 예측결과와 실험결과간에 유사성을 보임으로써 HUKINS 가 CANDU6 감속재 유동의 실험적 연구에 사용 가능함을 입증했다.

• Bulletin of the Korean Chemical Society
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• 제35권4호
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• pp.1191-1194
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• 2014

Imaging Differential Optical Absorption Spectroscopy (Imaging-DOAS) has been utilized in recent years to provide slant column density (SCD) distributions of several trace gas species in the plume. The present study introduces a new method using Imaging-DOAS data to determine two-dimensional plume structure from the plume emissions of power plant in conditions of negligible aerosol effects on radiative transfer within the plume. We demonstrates for the first time that two-dimensional distributions of sulfur dioxide ($SO_2$) and nitrogen dioxide ($NO_2$) in power plant emissions can be determined simultaneously in terms of SCD distribution. The $SO_2$ SCD values generally decreased with increasing distance from the stack and with distance from the center of the plume. Meanwhile, high $NO_2$ SCD was observed at locations several hundred meters away from the first stack due to the ratio change of NO to $NO_2$ in NOx concentration, attributed to the NO oxidation by $O_3$. The results of this study show the capability of the Imaging-DOAS technique as a tool to estimate plume dimensions in power plant emissions.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2002년도 학술대회지
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• pp.743-746
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• 2002

Experimental investigation of the flow field of supersonic cavity is described. In this research, supersonic cavity is used in chemical laser system. For efficient laser, downstream flow after cavity need to be uniform and clear for pressure recovery system. In previous research, it's known that there's oscillation In cavity and is due to Mach number and L/D ratio. A strong recompression occurs at the after wall and the flow is visibly unsteady. Cavity flow in this research is of the open type, that is, length-to-depth ratio $L/D<10\;at\;M\;=\;3$. Experiment is done with pressure measurement by piezo-type sensor and visualization by Schlirern method. The time-dependent experimental result is compared with computation.

• 대한의생명과학회지
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• 제8권3호
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• pp.161-165
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• 2002

Apoptosis can be difficult to detect in routine histological sections. Since extensive DNA fragmentation is an important characteristic of this process, visualization of DNA breaks could greatly facilitate the identification of apoptotic cells. Several techniques for the qualitative and quantitative detection of this process have been established; recently, an in situ nick end-labelling technique based on the detection of DNA fragmentation, which is a molecular characteristic of apoptotic cell death, was described. Applying this method to paraffin sections of rat tissues, sensitivity was observed to be inconsistently low with regard to the expected number of apoptotic cells. I describe a new modified method for formalin-fixed, paraffin-embedded tissue sections, pretense pretreatment to permeate the tissue sections that involves an TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) is acknowledged as a method of choice in the rapid identification and quantification of the apoptotic cell fraction in paraffin tissue preparations. TUNEL was performed without apoptosis and with apopotosis samples to each of the three concentrations of proteinase K (10, 25, 40 mg/ml) pretreatments. In this study, I show that chemical pretreatments of the tissue sections in proteinase K (25 mg/ml for 15 min at room temperature) considerably enhances the sensitivity of this nick end labelling technique.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2012년도 제38회 춘계학술대회논문집
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• pp.429-434
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• 2012

금속 연료로 사용되는 마이크로/나노 알루미늄 입자를 산화피막에 의한 점화 지연을 최소화 하는 점화 방법을 제시 하였다. 알루미늄 입자를 생성시킴과 동시에 가열하여 입자가 생성된 직후 산소와 접촉시 격렬한 산화 반응을 유도하여 점화를 시키는 방법이다. 1064 nm 파장의 Nd:YAG 펄스 레이저를 이용한 레이저 삭마(laser ablation)를 알루미늄 시편에 발생시켜 입자를 생성하였으며, 산란 기법(scattering method)을 이용하여 입자를 가시화하여 생성을 확인하였다. 10.6 ${\mu}m$ 파장의 $CO_2$ 연속 레이저를 사용하여 알루미늄 시편을 가열하고 생성된 입자의 점화 열원으로 사용하여 알루미늄 입자가 점화되고 연소되어 이동하는 궤적을 확인하였다.

• 에너지공학
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• 제22권1호
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• pp.28-37
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• 2013

교반 탱크(stirred tank)는 회전하는 임펠러(impeller)를 이용하여 단상 또는 다상의 유체를 지속적으로 유동시키는 장치로 여러 산업분야에 활용되고 있다. 우수한 성능의 교반기를 설계하기 위해서는 교반 성능에 영향을 미치는 다양한 내부유동특성의 정량적 데이터의 확보가 반드시 필요하지만, 복잡한 구조의 내부유동에 관한 정량적 해석은 현재까지 어려운 문제로 인식되고 있다. 본 연구에서는 전산유체 해석을 통해 교반 탱크에 적합한 기법을 제안하기 위해 Flunet 6.3의 두 가지 모델을 사용하였다. mixture model을 이용하여 교반 탱크 혼합을 해석하였으며, standard, k-${\varepsilon}$ model을 이용하여 교반 탱크 내의 유동을 해석하였다. 해석 기법으로는 다중 좌표계(Multiple Reference Frame)와 이동 격자(Sliding Mesh) 기법을 이용하였다. 전산유체해석 결과를 가시화 실험 결과와 비교하여 교반 탱크의 내부 유동 및 혼합 특성을 파악하고, 교반 탱크 내부 유동 해석 시 적절한 해석기법 선정의 기초자료를 제시하였다.

• 한국가시화정보학회:학술대회논문집
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• 한국가시화정보학회 2004년도 Proceedings of 2004 Korea-Japan Joint Seminar on Particle Image Velocimetry
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• pp.65-74
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• 2004

Measurement of concentration distributions of suspended particles in a micro-channel is out of the most crucial necessities in the area of Lab-on-a-chip to be used for various bio-chemical applications. One most feasible way to measure the concentration field in the micro-channel is using micro-LIF(Laser Induced Fluorescence) method. However, an accurate concentration field at a given cross plane in a micro-channel has not been successfully achieved so far due to various limitations in the light illumination and fluorescence signal detection. The present study demonstrates a novel method to provide an ultra thin laser sheet beam having five(5) microns thickness by use of a micro focus laser line generator. The laser sheet beam illuminates an exact plane of concentration measurement field to increase the signal to noise ratio and considerably reduce the depth uncertainty. Nile Blue A was used as fluorescent dye for the present LIF measurement. The enhancement of the fluorescent intensity signals was performed by a solvent mixture of water $(95\%)$ and ethanol (EtOH)/methanol (MeOH) $(5\%)$ mixture. To reduce the rms errors resulted from the CCD electronic noise and other sources, an expansion of grid size was attempted from $1\times1\;to\;3\times3\;or\;5\times5$ pixel data windows and the pertinent signal-to-noise level has been noticeably increased accordingly.

• Korea-Australia Rheology Journal
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• 제17권4호
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• pp.207-215
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• 2005

We present a finite difference solution for electrokinetic flow in rectangular microchannels encompassing Navier's fluid slip phenomena. The externally applied body force originated from between the nonlinear Poisson-Boltzmann field around the channel wall and the flow-induced electric field is employed in the equation of motion. The basic principle of net current conservation is applied in the ion transport. The effects of the slip length and the long-range repulsion upon the velocity profile are examined in conjunction with the friction factor. It is evident that the fluid slip counteracts the effect by the electric double layer and induces a larger flow rate. Particle streak imaging by fluorescent microscope and the data processing method developed ourselves are applied to straight channel designed to allow for flow visualization of dilute latex colloids underlying the condition of simple fluid. The reliability of the velocity profile determined by the flow imaging is justified by comparing with the finite difference solution. We recognized the behavior of fluid slip in velocity profiles at the hydrophobic surface of polydimethylsiloxane wall, from which the slip length was evaluated for different conditions.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2005년도 BIOINFO 2005
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• pp.215-220
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• 2005

Generally, enzymes in the starch biosynthesis pathway exist in many isoforms, contributing to the difficulties in the dissection of their specific roles in controlling starch properties. In this study, we present an algorithm as an alternative method to classify isoforms of starch biosynthesis enzymes based on their conserved secondary structures. Analysis of the predicted secondary structure of plant soluble starch synthase I (SSI) and soluble starch synthase II (SSII) demonstrates that these two classes of isoform can be reclassified into three subsets, SS-A, SS-B and SS-C, according to the differences in the secondary structure of the protein at C-terminus. SS-A reveals unique structural features that are conserved only in cereal plants, while those of SS-B are found in all plants and SS-C is restricted to barley. These findings enable us to increase the accuracy in the estimation of evolutionary distance between isoforms of starch synthases. Moreover, it facilitates the elucidation of correlations between the functions of each enzyme isoforms and the properties of starches. Our secondary structure analysis tool can be applicable to study the functions of other plant enzyme isoforms of economical importance.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2008년도 춘계학술대회논문집
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• pp.666-669
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• 2008

Droplet in miniaturized microfluidic systems have received much focused attention recently. In this work, electrical charging phenomenon of a conducting water droplet on the electrode under the dc electric field is studied and using this phenomenon droplet actuation method for microreactor applications is experimentally demonstrated. To find effects of key factors, the effects of electric field, medium viscosity, and droplet size are investigated. A scaling law of charging for the conducting droplet is derived from the experimental results. Unlike the case of a perfect conductor, the estimated amount of electrical charge ($Q_{est}$) of a water droplet is proportional to the 1.59 power of the droplet radius (R) and the 1.33 power of the electric field strength (E). (For a spherical perfect conductor, Q is proportional to R2 and E.) It is thought that the differences are mainly due to incomplete charging of a water droplet resulted from the combined effect of electrochemical reaction at electrode and the relatively low conductivity of water. Using this phenomenon, we demonstrate the transport of the charged droplet and fusion of two oppositely-charged droplets. When electric field is subjected sequentially on the electrode, the charged droplet is transported on the electrode. For the visualization of fusion of charged droplets, the precipitation reaction is used. When subjected to a DC voltage, two droplets charged are moving and merging toward each other due to the Coulombic force and chemical reaction is simultaneously occurred by coalescence of droplets. It may be due to the interchange effect of charge. It is shown that the droplet can be used for microreactor where transporting, merging etc. of reagents constitute unit operation.