• Asian-Australasian Journal of Animal Sciences
• /
• v.17 no.11
• /
• pp.1599-1607
• /
• 2004

This study examined the effects of pre-slaughter fasting, chasing stress and chiller ageing on objective meat quality, and their relations to the proteome profile of longissimus muscle using 20 male Korean native black pigs. Treatments were composed of two levels of pre-slaughter feed withdrawal, two levels of pre-slaughter stress and four chiller ageing times. A 15 min chasing stress immediately prior to slaughter significantly (p<0.05) decreased detectable levels of $\mu$-calpain activity during rigor development and chiller ageing, but did not have any direct effect on objective meat quality. On the other hand, pigs fed until the morning of slaughter resulted in significantly (p<0.05) higher hunter L* value and cooking loss than those which received an 18 h feed withdrawal prior to slaughter. Cooking loss and hunter L* value were constant during 7 d of chiller ageing, followed by significant increases at 14 d. The fed animals showed a significantly (p<0.05) higher hunter a* value at both 3 and 7 d, while the other group maintained a stable redness for 7 d. WB-shear force was not affected by the pre-slaughter treatments, but had significant (p<0.05) linear reduction from 1 to 7 d. A gelbased proteome analysis was performed on selected animals for low and high hunter L* values at 1 d. Ten and five spots had greater than two-fold spot densities for the low and high hunter L* groups, respectively. The ten spots included chain A, deoxyribounclease I complex with actin, heat shock protein 27 kDa, a protein similar to cardiac $Ca^{2+}$ release channel, and myosin heavy chain, while the five spots included chain A aldehyde dehydrogenase, glycerol-3 phosphate dehydrogenase, and hemoglobin alpha chain. In general, feeding until the morning of slaughter resulted in more desirable meat color, but appeared to reduce palatability due to increased cooking loss. Proteome analysis demonstrated that various proteins were concomitantly involved in the determination of final meat color. The most noticeable observation in the current study was that various isoforms for a particular protein differed in degradation and/or expression rate depending on meat quality.

• Journal of Korean Medical classics
• /
• v.20 no.4
• /
• pp.45-63
• /
• 2007

In this dissertation, I will focus on the channel entry, the effect and the treatment throughout books of oriental medicine from ancient to modern in order to classify the medicines of the Spleen as main or supplementary organ. The results are as follows: 1. The medicines which work on the Spleen(本臟) chiefly were 38, which were Gingseng Radix(人蔘), Astragali Radix, Hoelen, Atractylodis Rhizoma alba(白朮), Glycyrrhizae Radix(甘草), Atractyodis Rhizoma(蒼朮), Aurantii Nobilis Pericarpium(陳皮), Pinelliae Rhizoma(半夏), Nelumbinis Semen(蓮肉), Semen Euryacles, Crataegi Fructus, Dolichoris Semen(扁豆), Hordei Fructus Germinatus(麥芽), Dioscoreae Radix(山藥), Paeoniae Radix(白芍藥), Zingiberis Rhizoma(乾薑), Arecae Pericarpium(大腹皮), Cimicifugae Rhizoma(升麻), Aurantii Fructus(枳殼), Tiglii Semen(巴豆), Scirpi Rhizoma(三稜), Paeoniae Radix rubra(赤芍藥), Amydae Carapax(鱉甲), (Coptidis Rhizoma(黃連), Dioscoreae Radix(萎藥), Amomi Semen(砂仁), Zingiberis Rhizoma(生薑), Saussureae Radix(木香), Cinnamomi Cortex Spissus(肉桂), Myristicae Semen, Alpiniae Fructus(益智仁), Evodiae Fructus(吳萸), Caryophylli Flos(丁香), Agastachis Herba(藿香), Fructus Piperis Nigri Seu Albi(胡椒), Acontii Tuber(附子), Alpiniae Officinari Rhizoma(良薑), Fructus Galangae. 2. The medicines which work on the other viscera(他臟) chiefly were 12, which were Talcum(滑石), Bupleuri Radix(柴胡), Semen Lepidii Seu Descurainiae, Mori Cotex Radicis(桑白皮), Aurantii lmmaturi Pericarpium(靑皮), Gardeniae Fructus(梔子), Forsythiae Frucus(連翹), Antelopis cornu(羚羊角), Alimatis Rhizoma(澤瀉), Epimedii Herba(仙靈脾), Cyperi Rhizoma(香附子), Rhizome Chuanxiong(川芎). 3. medicines, effected on the Spleen functioned through any other viscera were as follows: Talcum(滑石) works to treat renal heat Entering the Spleen(腎熱入脾) Bupleuri Radix(柴胡) works to treat Hepatic Asthenia Entering the Spleen(肝虛入脾) Semen Lepidii Seu Descurainiae and Mori Cotex Radicis(桑白皮) works to treat Pulmonary gi Entering the Spleen(肺氣入脾) Aurantii lmmaturi Pericarpium(靑皮) works to treat Hepatic gi Entering the Spleen(肝氣入脾) Gardeniae Fructus(梔子) and Forsythiae Frucus(連翹) works to treat Cardiac Heat Entering the Spleen(心熱入脾) Antelopis cornu(羚羊角) works to treat Hepatic wind Entering the Spleen(肝風人脾) Alimatis Rhizoma(澤瀉) works to treat Hepatic heat Entering the Spleen(肝熱入脾) Epimedii Herba(仙靈脾) works to treat Renal asthenia Entering the Spleen(腎虛入脾) Cyperi Rhizoma(香附子) 와 Rhizome Chuanxiong(川芎) works to treat Hepatic gi Entering the Spleen(肝氣入脾) In the study of concerning the medicines effected on the spleen, It is considered that it dedicated to development of the medicines related to the spleen and making efficient use of the medicines.

• The Journal of the Acoustical Society of Korea
• /
• v.37 no.1
• /
• pp.31-38
• /
• 2018

Based on the measured data in the south western sea of Jeju Island during the SAVEX15(Shallow Water Acoustic Variability EXperiment 2015), the effect of internal waves on the PPD (Predictive Probability of Detection) of a sonar system was analyzed. The southern west sea of Jeju Island has complex flows due to internal waves and USC (Underwater Sound Channel). In this paper, sonar performance is predicted by probabilistic approach. The LFM (Linear Frequency Modulation) and MLS (Maximum Length Sequence) signals of 11 kHz - 31 kHz band of SAVEX15 data were processed to calculate the TL (Transmission Loss) and NL (Noise Level) at a distance of approximately 2.8 km from the source and the receiver. The PDF (Probability Density Function) of TL and NL is convoluted to obtain the PDF of the SE (Signal Excess) and the PPD according to the depth of the source and receiver is calculated. Analysis of the changes in the PPD over time when there are internal waves such as soliton packet and internal tide has confirmed that the PPD value is affected by different aspects.

• The KIPS Transactions:PartC
• /
• v.17C no.1
• /
• pp.1-14
• /
• 2010

IEEE 802.15.4[1] has been standardized for the physical layer and MAC layer of LR-PANs(Low Rate-Wireless Personal Area Networks) as a technology for operations with low power on sensor networks. The standardization is applied to the variety of applications in the shortrange wireless communication with limited output and performance, for example wireless sensor or virtual wire, but it includes vulnerabilities for various attacks because of the lack of security researches. In this paper, we analyze the vulnerabilities against the denial of sleep attacks on the MAC layer of IEEE 802.15.4, and propose a detection mechanism against it. In results, we analyzed the possibilities of denial of sleep attacks by the modification of superframe, the modification of CW(Contention Window), the process of channel scan or PAN association, and so on. Moreover, we comprehended that some of these attacks can mount even though the standardized security services such as encryption or authentication are performed. In addition to, we model for denial of sleep attacks by Beacon/Association Request messages, and propose a detection mechanism against them. This detection mechanism utilizes the management table consisting of the interval and node ID of request messages, and signal strength. In simulation results, we can show the effect of attacks, the detection possibility and performance superiorities of proposed mechanism.

• The Korean Journal of Physiology
• /
• v.26 no.1
• /
• pp.55-68
• /
• 1992

The effects of changes in extracellular $Na^+\;and\;Ca^+$ concentration on the membrane potential and contractility were studied in the antral circular muscle of guinea pig stomach in order to elucidate the existence and the nature of $Na^+/Ca^{2+}$ exchange mechanism. All experiments were performed in tris buffered Tyrode solution which was aerated with 100% $O_2$ and kept at $35^{\circ}C.$ The treatment of $10^{-5}$ ouabain was performed to induce intracellular $Na^+$ loading prior to the start of experiment. The results were as follows: 1. $Na^+$-free Tyrode or high $Ca^{2+}$-Tyrode solution hyperpolarized the membrane potential and induced contracture. The time course of contracture was similar to that of change in membrane potential. 2. The degree of hyperpolarization and the amplitude of contracture decreased in accordance with the increase of extracellular $Na^+$ concentration. 3. $Na^+$-free contracture was developed even after blocking the influence of intrinsic nerves by the pretreatment with atropine, guanethidine and TTX. 4. $Ca^{2+}$-channel blockers(D-600 or $Mn^{2+}$) and the blocker of intracellular $Ca^{2+}$ release from sarcoplasmic reticulum(ryanodine) did not suppress the development of $Na^+$-free contracture. And also, dinitrophenol had no effect on $Na^+$-free contracture. 5. Dose-response relationship between extracellular $Na^+$ concentrations and the magnitude of contractures showed a sigmoid pattern. The slope of straight line from Hill plot was 2.7. 6. In parallel with the increase of extracellular $Ca^{2+}$ concentration, the amplitude of contracture increased dose dependently and was maximum at 8 mM $Ca^{2+}$-Tyrode solution. 7. The relationship between extracellular $Ca^{2+}$ concentrations and the magnitude of contractures showed hyperbolic pattern. The slope of straight line from Hill plot was 1.1. From the above results, it is suggested that $Na^+/Ca^{2+}$ exchange mechanism exists in the antral circular muscle of guinea pig stomach and this mechanism affects the membrane potential electrogenically.

• Journal of Distribution Science
• /
• v.14 no.6
• /
• pp.89-98
• /
• 2016

Purpose - This article tackles risk communication issues and aims to address the characteristics of MERS risk information distribution in South Korea, and secondly to examine the communicative behavior of the public health authority in terms of the quality of communication strategies. Thirdly, the study attempts to figure out the risk communication to cope with MERS through the applications of SMCRE model in chronological order. We employ the social amplification of risk framework for analyzing the emergent public response as one of the main approaches. Research Design, Data and Methodology - The main framework of this study is theoretically based on the social amplification of risk, which describes signals about risk transmitted and processed by individuals and social groups. The model also reflects the interactions between social groups and institutes about disaster-related risk issues, which are potential amplifiers or attenuators of communication signals. S-M-C-R-E Model is methodologically employed to examine the social amplification for MERS risk information in each period, which we defined operationally. The proposed methodology allows the assessment of effectiveness and ineffectiveness on risk communication to be conceptualized as a countermeasure against disasters. The paper focuses on exploring how social risk amplification can be applied and organized in each stage. Results - The SMCRE model describes the exchange of risk information and is also applied to all forms of communication between stakeholders including public health authority, local government and media. Each factor of risk communication includes source, message, channel, receiver and effect. The results support that the effective risk communication involves not only the improved reliability of public health authority as a key factor of risk communication, but also a close cooperation and good collaboration with local governments. It does not seem to be possible that the government-initiated risk communication based on controllability and management cope effectively with infectious disease in early stage. The results of this study imply that the shared risks between local, regional and national authorities can enhance risk communication system. Conclusions - The study supports that the disparities in how disaster-related risk information is interpreted and coded, have made effective risk communication and public sense-making impeded. Our findings support a more communicative discussion about the role of risk information sharing between governments for the improvement of emergency management and underline the importance of social elements in the risk communication, such as relationship and trust building. Findings suggest that trust building between stakeholders could be added to help explain the processes of social amplification and attenuation of risk. It would be recommended that the continuous risk communication with all the involved stakeholders will be able to help national health promotion policy to be improved regarding emergency management. Furthermore, risk communication has to be a scientific approach for the communication pertaining to potentially sensitive or controversial situations with public concerns and low public trust.

• The Korean Journal of Physiology and Pharmacology
• /
• v.4 no.6
• /
• pp.471-477
• /
• 2000

In the rabbit renal artery, acetylcholine $(ACh,\;1\;nM{\sim}10\;{\mu}M)$ induced endothelium-dependent relaxation of arterial rings precontracted with norepinephrine $(NE,\;1\;{\mu}M)$ in a dose-dependent manner. $N^G-nitro- L-arginine$ (L-NAME, 0.1 mM), an inhibitor of NO synthase, or ODQ $(1\;{\mu}M),$ a soluble guanylate cyclase inhibitor, partially inhibited the ACh-induced endothelium-dependent relaxation. The ACh-induced relaxation was abolished in the presence of 25 mM KCl and L-NAME. The cytochrome P450 inhibitors, 7- ethoxyresorufin $(7-ER,\;10\;{\mu}M),$ miconazole $(10\;{\mu}M),$ or 17-octadecynoic acid $(17-ODYA,\;10\;{\mu}M),$ failed to inhibit the ACh-induced relaxation in the presence of L-NAME. 11,12-epoxyeicosatrienoic acid $(11,12-EET,\;10\;{\mu}M)$ had no relaxant effect. The ACh-induced relaxation observed in the presence of L-NAME was significantly reduced by a combination of iberiotoxin $(0.3\;{\mu}M)$ and apamin $(1\;{\mu}M),$ and almost completely blocked by 4-aminopyridine (5 mM). The ACh-induced relaxation was antagonized by $P_{2Y}$ receptor antagonist, cibacron blue $(10\;and\;100\;{\mu}M),$ in a dose-dependent manner. Furthermore, 2-methylthio-ATP (2MeSATP), a potent $P_{2Y}$ agonist, induced the endothelium-dependent relaxation, and this relaxation was markedly reduced by either the combination of iberiotoxin and apamin or by cibacron blue. In conclusion, in renal arteries isolated from rabbit, ACh produced non-NO relaxation that is mediated by an EDHF. The results also suggest that ACh may activate the release of ATP from endothelial cells, which in turn activates $P_{2Y}$ receptor on the endothelial cells. Activation of endothelial $P_{2Y}$ receptors induces a release of EDHF resulting in a vasorelaxation via a mechanism that involves activation of both the voltage-gated $K^+$ channels and the $Ca^{2+}-activated\;K^+\;channels$. The results further suggest that EDHF does not appear to be a cytochrome P450 metabolite.

• The Korean Journal of Physiology and Pharmacology
• /
• v.20 no.1
• /
• pp.9-14
• /
• 2016

Adenosine 3',5'-cyclic monophosphate (cAMP) participates in the regulation of numerous cellular functions, including the $Na^+-K^+$-ATPase (sodium pump). Ouabain, used in the treatment of several heart diseases, is known to increase cAMP levels but its effects on the atrium are not understood. The aim of the present study was to examine the effect of ouabain on the regulation of atrial cAMP production and its roles in atrial endothelin-1 (ET-1) secretion in isolated perfused beating rabbit atria. Our results showed that ouabain ($3.0{\mu}mol/L$) significantly increased atrial dynamics and cAMP levels during recovery period. The ouabain-increased atrial dynamics was blocked by KB-R7943 ($3.0{\mu}mol/L$), an inhibitor for reverse mode of $Na^+-Ca^{2+}$ exchangers (NCX), but did not by L-type $Ca^{2+}$ channel blocker nifedipine ($1.0{\mu}mol/L$) or protein kinase A (PKA) selective inhibitor H-89 ($3.0{\mu}mol/L$). Ouabain also enhanced atrial intracellular cAMP production in response to forskolin and theophyline ($100.0{\mu}mol/L$), an inhibitor of phosphodiesterase, potentiated the ouabain-induced increase in cAMP. Ouabain and 8-Bromo-cAMP ($0.5{\mu}mol/L$) markedly increased atrial ET-1 secretion, which was blocked by H-89 and by PD98059 ($30{\mu}mol/L$), an inhibitor of extracellular-signal-regulated kinase (ERK) without changing ouabain-induced atrial dynamics. Our results demonstrated that ouabain increases atrial cAMP levels and promotes atrial ET-1 secretion via the mitogen-activated protein kinase (MAPK)/ERK signaling pathway. These findings may explain the development of cardiac hypertrophy in response to digitalis-like compounds.

• The Korean Journal of Physiology and Pharmacology
• /
• v.20 no.1
• /
• pp.101-109
• /
• 2016

Reducing $[Mg^{2+}]_o$ to 0.1 mM can evoke repetitive $[Ca^{2+}]_i$ spikes and seizure activity, which induces neuronal cell death in a process called excitotoxicity. We examined the issue of whether cultured rat hippocampal neurons preconditioned by a brief exposure to 0.1 mM $[Mg^{2+}]_o$ are rendered resistant to excitotoxicity induced by a subsequent prolonged exposure and whether $Ca^{2+}$ spikes are involved in this process. Preconditioning by an exposure to 0.1 mM $[Mg^{2+}]_o$ for 5 min inhibited significantly subsequent 24 h exposure-induced cell death 24 h later (tolerance). Such tolerance was prevented by both the NMDA receptor antagonist D-AP5 and the L-type $Ca^{2+}$ channel antagonist nimodipine, which blocked 0.1 mM $[Mg^{2+}]_o$-induced $[Ca^{2+}]_i$ spikes. The AMPA receptor antagonist NBQX significantly inhibited both the tolerance and the $[Ca^{2+}]_i$ spikes. The intracellular $Ca^{2+}$ chelator BAPTA-AM significantly prevented the tolerance. The nonspecific PKC inhibitor staurosporin inhibited the tolerance without affecting the $[Ca^{2+}]_i$ spikes. While $G{\ddot{o}}6976$, a specific inhibitor of $PKC{\alpha}$ had no effect on the tolerance, both the $PKC{\varepsilon}$ translocation inhibitor and the $PKC{\zeta}$ pseudosubstrate inhibitor significantly inhibited the tolerance without affecting the $[Ca^{2+}]_i$ spikes. Furthermore, JAK-2 inhibitor AG490, MAPK kinase inhibitor PD98059, and CaMKII inhibitor KN-62 inhibited the tolerance, but PI-3 kinase inhibitor LY294,002 did not. The protein synthesis inhibitor cycloheximide significantly inhibited the tolerance. Collectively, these results suggest that low $[Mg^{2+}]_o$ preconditioning induced excitotoxic tolerance was directly or indirectly mediated through the $[Ca^{2+}]_i$ spike-induced activation of $PKC{\varepsilon}$ and $PKC{\xi}$, JAK-2, MAPK kinase, CaMKII and the de novo synthesis of proteins.

• Korean Journal of Veterinary Research
• /
• v.46 no.4
• /
• pp.323-326
• /
• 2006

Suaeda (S.) asparagoides $M_{IQ}$, one of the halophyte groups, has been used as a folk remedy for digestive disturbances in Korea. However, its pharmacological activity on gastrointestinal motility has not been reported yet. In this study, the effects of this halophyte extracts with various solvent fractions (ethanol, hexane, chloroform, ethyl acetate, butanol, and water) on mice ileal spontaneous motility was examined. All solvent fractions at the concentration of $100{\mu}g/ml$ showed inhibitory actions on spontaneous motility of ileum with the potency order of water > 70% ethanol > hexane ${\gg}$ chloroform ${\geq}$ butanol ${\geq}$ ethyl acetate, respectively. In addition, the water fraction of extracts from S. asparagoides $M_{IQ}$ (WFSA) dose-dependently ($1-100{\mu}g/ml$) inhibited the amplitude of spontaneous phasic contraction and area under the contractile curve (AUC). The inhibitory effect of water fraction at the concentration of $10{\mu}g/ml$ was not affected by tetrodotoxin (TTX), $Na^+$ channel blocker ($1{\mu}M$), and $N^w$-nitro-L-arginine Methyl Ester (L-NAME), nitric oxide synthase inhibitor ($100{\mu}M$). However, cyclopiazonic acid (CPA, $10{\mu}M$), inhibitor of sarcoplasmic reticulum $Ca^{2+}$-ATPase, almost blocked the inhibitory effects of WFSA ($10{\mu}g/ml$) on the spontaneous phasic contraction of mouse ileum. But, CPA did not inhibit the lowering basal tone effects of WFSA. The result of this study showed that various extracts of S. asparagoides $M_{IQ}$ induce inhibitory effects on spontaneous contraction of mice ileal segments. More over, the polar solvent fractions were shown to be more potent than non-polar solvent fractions. The effects of S. asparagoides $M_{IQ}$ extracts are not mediated by nerve or nitric oxide. The inhibitory effects of WFSA at least partially mediated by sarcoplasmic reticulum $Ca^{2+}$-ATPase. However, further study is required to determine the exact pharmacological mechanisms of this halophyte on its gastrointestinal motility inhibitory effects.