• The Journal of Korean Academy of Prosthodontics
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• v.37 no.4
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• pp.425-432
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• 1999

The purpose of this study is to evaluate the color differences that can exist between different brands of dentin porcelain having identical shade designations. The instrumental colorimetric technique was used to determine these differences. The three brands of dentin porcelain used were Vita VMK-68, Shofu Vintage, and Ceramco II. The three shades selected were the Vita Lumin shades A2, B2, and C2. The color difference values were compared with each other using $L^*,\;a^*,\;b^*$, three variables of the CIELAB color system, and ${\Delta}E$. The results of this study were as follows: 1 Corresponding shades of different brands of dentin porcelain produced different $L^*,\;a^*,\;b^*$ values. 2. The greatest ${\Delta}E$ value in corresponding shade was found to exist between Ceramco II and Shofu Vintage, followed by between Vita VMK-68 and Ceramco II, and then between Vita VMK-68 and Shofu Vintage. 3. The ${\Delta}E$ values in shade C2 were mainly influenced by ${\Delta}L^*$ value ; however none of ${\Delta}L^*,\;{\Delta}a^*,\;{\Delta}b^*$ values had greater influence on ${\Delta}E$ values than the others in shade A2, B2. 4. Shofu Vintage had the highest $b^*$ value, followed by Vita VMK-68, and then Ceramco II.

• Journal of Life Science
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• v.24 no.8
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• pp.843-850
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• 2014

In the current study, we prepared eighty-five different kinds of solvent fractions of rice wine lees and nuruk extracts and investigated their effects on cell viability and nitric oxide (NO) production in mouse RAW 264.7 cells. Among the treated solvent fractions, only three solvent fractions (KSD-E1-3, KSD-E2-3 and KSD-E4-3) significantly decreased NO production in LPS-activated RAW 264.7 cells without affecting cell viability. And, they also reduced the expression of pro-inflammatory genes such as COX-2, TNF-alpha and iNOS. To understand the molecular mechanisms involved in the inhibition of inflammation in (KSD-E4-3)-treated RAW 264.7 cells, we carried out oligo DNA microarray analysis using Agilent Mouse microarray. To confirm microarray data, 6 genes (IL-1F6, iNOS, IL-10, Fabp4, IL-1RN and CSF2) were selected and performed RT-PCR and quantitative real-time PCR analysis with gene specific primers. The results of RT-PCR and real-time PCR agreed with microarray data. Overall, our results suggest that rice wine lees can be a novel resource for the development of foods and drugs which possess anti-inflammatory activity.

• Proceedings of the Korea Institute of Applied Superconductivity and Cryogenics Conference
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• 1999.02a
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• pp.65-68
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• 1999

The major deisng parameters that are considered in this paper are: 1) EMF according to width of field coil. 2) EMF according to magnetic shield length. Because of superconducting generator (SG) is actually and air cored machine with its rotor iron and stator iron teeth having been removed. In this case, the desing of the SG must be based on the 3D analysis of the magnetic field. This study presents an effective armature winding type with 3D FEM(Finite Element Method), and compares analyzed and measured results.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.199.3-199.3
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• 2003

Acanthopanacis Cortex (Acanthopanax sessiliflorum, Araliaceae, KP VIII), an important Korean medicinal herbal drug, has been widely used as tonic, anti-stress and immuno-enhancing drugs. To monitor the contents of active ingredients (acanthoside D[=eleutheroside E], eleutheroside B, isofraxidin, chlorogenic acid, and caffeic acid) in Acanthopanax sp., we developed the HPLC analysis method and validated. The simultaneous determination of five active ingredients was achieved in a C$\sub$18/ column with an acetonitrile water (containing 1 % phosphoric acid) (15 : 85) mobile phase. (omitted)

• Journal of Microbiology and Biotechnology
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• v.7 no.5
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• pp.299-304
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• 1997

Expression in the periplasm of Escherichia coli of cloned heavy and light chain cDNAs for Fab fragment of a murine monoclonal antibody MabB9 (${\gamma}2b$, K), specific for human plasma apolipoprotein B-100 of LDL, was studied. For the purpose, a vector for two-cistronic expression of the heavy chain cDNA, at the 5' terminus, and light chain cDNA, at the 3' terminus, was constructed using the signal sequences, pelB (for heavy chain) and ompA (for light chain) in a pET vector system. The constructed vector was transformed into E. coli BL21(DE3). The expressed heavy chain (25 kDa) and light chain (23 kDa) of the antibody molecule were detected in total cell extracts as well as in the periplasmic extracts of E. coli.

• Journal of Auto-vehicle Safety Association
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• v.3 no.2
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• pp.11-16
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• 2011

In recent years, rapid-increasing market share of compact cars and SUVs has brought for both consumer and automaker to pay more attention on crash compatibility between the compact passenger vehicles and the light trucks (i.e., Pickups and SUVs). Vehicle compatibility regarding both self and partner protection in frontal crash of different class vehicles is one of hot issues in vehicle safety. Furthermore, it is expected that the amendment of UNECE-Regulation 94 to implement compatibility issues in couple of coming years. In this study, conceptual design of compatibility compliant frontal vehicle structure which subjects to improve? the distribution of frontal crash loading and structural engagement between vehicles is introduced. The effects of proposed vehicle structure on both possible candidates (i.e. FWRB, FWDB and PDB) for a compatibility evaluation test procedure and car-to-car crash are also investigated.

• International Journal of Internet, Broadcasting and Communication
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• v.12 no.3
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• pp.108-115
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• 2020

Blended learning is a teaching method utilizing all the advantages in 'on and off-line' learning circumstances in order to enhance the learning effect and efficiency, more than the simple use of online factors in the classroom education. In this paper, we present the realization and simulation of algorithm for the realtime evaluation of low-grade and high-grade subjects in order to implement smart e-learning system, considering a lecture intelligibility. In order to grasp the levels of student's intelligibility, we simulated a function that automatically summarizes the study contents of class given by a lecturer. Especially, in administrator mode of smart e-learning system, we suggested and simulated a system in order to help the lecturer to easily manage the student's grades, and we have provided software to tell the student's intelligibility of lecture, analyzed the rate of incorrect answers, automatic judgment of lecture intelligibility and judge the weakest subject.

• Journal of the Korea Concrete Institute
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• v.16 no.3 s.81
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• pp.415-423
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• 2004

To improve the brittle column behavior during seismic excitation, benefits of using steel fiber reinforced concrete in columns were investigated. For experimental study, eight specimens were used to evaluate the shear enhancement effect. The variables in this study were amount of shear reinforcement ratio (i.e., 0.26, 0.21 $\%$) and steel fiber volume fraction (i.e., 0.0, 1.0, 1.5, 2.0$\%$). The test results indicated that the maximum enhancement of shear capacity was shown in $1.5\%$ steel fiber content. In addition, to predict the maximum shear strength, equations of ACI 318-99, AIJ MB, NZS 3101, Hirosawa and Priestley were reviewed. From the parametric and regression study, modified Priestely equation was proposed by adding steel fiber effect.

• Asian Journal of Atmospheric Environment
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• v.9 no.3
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• pp.222-227
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• 2015

The environmental education with ideal teaching tools (e.g., a small-scaled experimental kit) is essential for raising the public's, especially student's, awareness of many environmental issues. This study was undertaken to develop a do-it-yourself model instrument of acid rain and estimating its applicability to the environmental education. Our novel model tool consists of a body (17 cm width, 6 cm depth, and 17 cm height), an acid gas injector, acid four rain reservoirs, and a drop supply pump. An alternative model instrument that can be more readily assembled was also suggested. Based on our novel approach, we carried out several model experiments with educatees (i.e., high school and college students, and adults). The results of questionnaires indicated that educatees achieved a very deep understanding of acid rain through an experienced model experiment. A 77 to 89% of educatees experienced the reappearance experiment of acid rain by our model instrument felt that it was very useful as a teaching tool of environmental education.

• Biotechnology and Bioprocess Engineering:BBE
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• v.2 no.2
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• pp.86-89
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• 1997

Epidermal growth factor(EGF) known as a urgastrone is a powerful mitogen with a wide variety of possibilities for medical usages. A mature EGF coding region was isolated from human prepro-EGF sequence by a conventional PCR and cloned into pQE vector in which the gene product was supposed to be expressed with 6$\times$His tag for the subsequent purification. The recombinant mature EGF was expressed in M15[Rep4], an Escherichia coli host strain, in amount of 30-40% of total proteins pressent in E. coli extract by the addition of isopropylthio-$\beta$-galactopyranoside (IPTG). The recombinant EGF purified using a Ni2+-NTA affinity colume chromatography was active in its ability to induce phosphorylation on tyrosine residues of several substrate proteins when murine NH3T3 and human MRC-5 fibroblast cells were stimulated with it. This work may provide the basic technology and information for the production of recombinant EGF.