• Fashion & Textile Research Journal
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• v.19 no.4
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• pp.486-492
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• 2017

This study finds surface color and functions of natural dyeing from Chamaecyparis obtusa leaves which help cure skin problems such as atopic dermatitis. This study also seeks to find dyeing properties and functions of natural colorants derived from Chamaecyparis obtusa and Rubia tinctorum on silk. Dyeing properties and functions are compared between silks dyed with Chamaecyparis obtusa and silks composite-dyed with Chamaecyparis obtusa and Rubia tinctorum. The comparison examines the feasibility of developing healthy and high functional fabrics and wellness of fashion merchandises. Silk dyeing with Chamaecyparis obtuse leaves yields yellow and double coloring from Chamaecyparis obtusa; however, Rubia tinctorum yields yellow/red on silks. Silks dyed with Chamaecyparis obtusa and silks composite-dyed with Chamaecyparis obtusa and Rubia tinctorum show a high level on dry cleaning, ultraviolet protection and deodorization. Especially, the dyed silks from leaves of Chamaecyparis obtusa show a 99.7% antimicrobial effect against staphylococcus aureus ATCC 653B. Silks composite-dyed with Chamaecyparis obtusa and Rubia tinctorum are better known for medicinal herb for dermatitis, and natural colorant, Rubia tinctorum does not improve significantly functions compared with silks dyed with Chamaecyparis obtusa. However, the dyeing properties improve by composite dyeing. This implies that ways to maximize effects of tie-dyeing technique could be developed.

• KSBB Journal
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• v.17 no.4
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• pp.357-364
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• 2002

For the effecient extraction methods of essential oil, pretreatment of leaves, ratios of water and leaves, extraction time, and collection season from the Chamaecyparis obtusa and Chamaecyparis pisifera leaves were studied. The chemical composition of essential oil was analyzed by GC-MS. The yield of essential oil from ground leaves was higher than that of chopped leaves. The yield of essential oil was not affected much by mixing ratios of water and leaves. The yield of essential oil reached maximum after 5 hours. The content of essential oil of C. obtusa leaves collected during winter was 4.5%, whereas the content of essential oil of C. pisifera collected during fall was 5.3%. The composition of essential oils extracted form C. obrusa and C. pisifera was different. The major constituents in the essential oil of C. obtusa were monoterpene as limonene, terpinene-4-ol, ${\gamma}$-selinene, and a-cedrene, and those of C. pisifera was monoterpens as ${\alpha}$-pinene, myrcene, limonene, bornyl acetate, ${\beta}$- caryophyllene, longifolene, and ${\beta}$-cedrene.

• Journal of Forest and Environmental Science
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• v.18 no.1
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• pp.53-60
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• 2001

2kg of the dried leaves of chamaecyparis obtusa Endlicher were ground, extracted with acetone-$H_2O$(7:3, v/v), concentrated, and fractionated with a series of hexane, $CH_2Cl_2$, EtOAc and water on a separatory funnel. Each fraction was freeze dried to give dark-brown powder and a EtOAc soluble portion of the powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol and ethanol-hexane mixture as eluents. Spectrometric analyses such as NMR and FAB-MS including TLC were performed to characterize the structures of the isolated compounds. The leaves of chamaecyparis obtusa Endlicher contained a large amount of taxifolin-3-O-${\beta}$-D-xylopyranoside and (+)-catechin in addition to a small amount of quercetin-3-O-${\alpha}$-L-rhamnopyranoside.

• Fashion & Textile Research Journal
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• v.19 no.3
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• pp.331-336
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• 2017

Bacteria exist everywhere and continuously come into contact with daily surroundings and humans. Super bacterium methicillin-resistant Staphylococcus aureus, resistant to methicillin, has recently appeared. The morbidity and rate of death associated with super bacteria infection has increased. This study investigated the antibacterial activity of fabrics naturally dyed with Chamaecyparis obtusa leaves extract against methicillin-resistant Staphylococcus aureus. Fabrics were left for 15 min in a natural dyeing solution prepared by extraction from C. obtusa leaves using 11.3% (o.w.f) with a fixed liquor ratio of 1:22 at $40^{\circ}C$. The dyeing process was conducted using three different mordants; subsequently, the K/S value of the dyed fabrics increased in the order of None < Cu < Fe < Al. The color fastness property of the fabrics to washing, dry-cleaning, and rubbing was found to be excellent and ranked in the 4-5 grade. The color fastness to light of natural dyeing is low in most cases and has the problem that the dye color soon becomes bleached. Yet, in most cases cloth dyed with retinispora leaves, the color fastnezz to light was good with a third to fourth grade. Non-mordant fabrics, aluminum mordants, and copper mordants also showed better antibacterial properties (99.9% reduction) against methicillin-resistant Staphylococcus aureus, compared to the control fabrics. The dyed fabrics showed the same antibacterial activity even after three washes. The results highlight the strong potential of fabrics naturally dyed with C. obtusa-extract as a medicinal material with excellent antibacterial function against methicillin-resistant Staphylococcus aureus.

• Journal of Applied Biological Chemistry
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• v.48 no.1
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• pp.26-28
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• 2005

Evaluation of larvicidal activities of Chamaecyparis obtusa and Thuja orientalis oils against 4th-instar larvae of Aedes aegypti and Culex pipiens pallens revealed larvicidal activities of leaf oils extracted from C. obtusa and T. orientalis were significantly higher than those of stem, fruit, and seed oils. Strong mortality was observed in age class II of C. obtusa and T. orientalis against Ae. aegypti and Cx. pipiens pallens larvae. These results show both leaf part and age class II of C. obtusa and T. orientalis have strong larvicidal activity against Ae. aegypti and Cx. pipiens pallens. Leaf oils of C. obtusa and T. orientalis leaves show promise as natural larvicides against Ae. aegypti and Cx. pipiens pallens.

• Applied Biological Chemistry
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• v.44 no.2
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• pp.116-121
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• 2001

The compositions of essential oils isolated from the leaves and fruits of Chamaecyparis obtusa (Sieb. et Zucc). Endl. and Chamaecyparis pisifera (Sieb. et Zucc.) Endl. were analyzed through GC and GC-MS. The oil yields were 0.83% (as fresh weight) and 1.36% in the leaves and the fruits of C. obtusa, and were 0.92% and 1.28% in those of C. pisifera, respectively. More than 90 components were identified, including high contents of monoterpenoids and sesquiterpenoids. Contents of monoteipenoids in the leaf and fruit oils of C. pisifera were higher than in those of C. obutsa. The major constituents in the leaf oil of C. obtusa were sabinene (11.81% as determined through GC peak area), limonene (7.73%), bornyl acetate (6.92%), $borneol+{\alpha}-teirineol$ (15.67%), and elemol (12.82%), and those in the fruit oil were myrcene (8.12%), ${\gamma}-terpinene$(5.91%), p-cymene(7.62%), $borneol+{\alpha}-terpineol$(6.53%) and ${\beta}-caryophyllene$ (23.74%). The major constituents in the leaf oil of C. pisifera were ${\alpha}-pinene$(32.34%), ${\delta}-3-carene$(25.28%), myrcene(11.72%), and bornyl acetate (8.77%), and those in the fruit oil were ${\alpha}-pinene$ (29.38%), ${\delta}-3-carene$(30.27%), myrcene(15.05%), and limonene(8.10%).

• Journal of the Korean Applied Science and Technology
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• v.37 no.6
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• pp.1496-1506
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• 2020

This study was conducted as follows in order to find out the possibility of using (Chamaecyparis obtusa) by part as a cosmetic material. Chamaecyparis obtusa was separated leaves, bark, wood, and root, and extracted with 99.9% ethanol, and the antioxidant and whitening effects of the sample were confirmed. For this, the following studies were conducted. Antioxidant evaluation was confirmed by 1 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS+) radical scavenging activity. To evaluate the whitening effect, mushroom tyrosinase inhibitory activity evaluation and cytotoxicity evaluation of Chamaecyparis obtusa extract through MTT assay were conducted, and cellular tyrosinase inhibition rate was measured and melanin contents was confirmed. As a result of antioxidant activity, Bark extract among Chamaecyparis obtusa parts showed the best efficacy, and bark in B16F10 melanoma cell showed tyrosinase activity inhibition and melanin contents inhibitory effects. Therefore, it was confirmed that the ethanol extract of Chamaecyparis obtusa was developed as a natural material for safe and excellent whitening functional cosmetics.

• The Korean Journal of Ecology
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• v.23 no.4
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• pp.323-329
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• 2000

The allelopathic effects of volatile substances from Chamaecyparis obtusa (S. et Z.) Endl. were examined on the germination and seedling growth of some plant species, and on the population growth of some microorganisms. The germination and seedling growth of the receptor plants were suppressed more severely by leaf and fruit essential oils than by those of other parts. Colonial growth of fungi was severely inhibited by essential oils extracted from leaves and fruits. The development of root hairs of the receptor plants was also severely inhibited by the essential oils. The cortical cells at the root tips of Lactuca sativa L. treated with essential oils showed contraction of the cytoplasm, resulting in plasma membranes becoming detached from the cell walls and the cells metamorphosing irregularly. Accumulation of lipid granules inside the contracted cytoplasm and degeneration of mitochondrial cristae were also observed.

• Journal of Korea Foresty Energy
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• v.7 no.1
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• pp.1-10
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• 1987

This study was carried out to estimate the aboveground biomass of Cryptomeria japonica and Chamaecyparis obtusa which was planted in Changsong district, the southern part of the Korean peninsula. Nine sample trees at each plot(20m x 20m drom C. japonica of 20-year-old and C. obtusa of 25-year-old, according to DBH distribution. were felled to measure the dry weights of stem, branches and leaves sectioned respectively. Since it is very difficult to separate leaves from twigs. all green parts including pure leaves and twigs were tentatively treated as 'leaves'. 1. The logarithmic regression equations between dry weight of each component (stem. branches. leaves land the variable of $(DBH)^2{\cdot}H$ were obtained (Table 6). The aboveground standing crops was estimated 108. 75ton/ha in C. japonica and 112.56 ton/ha in C. obtusa. Percentage of each part based on the aboveground standing crop in stand was and in order of stem >leaves>branches for C. japonica and in order of stem> branches> leaves for C. obtusa repectively. 2. Net production of each stand was estimated as 1:3.32ton/ha/yr in C. japonica and 11.69ton/ha/yr in C. obtusa. and its composition was in order of stem>leaves>branches for both species. 3The net assimilation rate was estimated as 1.10 and 1.2lkg/kg/yr, the efficiency of leaves to produce stem was 0.71 and 0.75 kg/kg/ha and biomass accumulation ratio was 8.16 and 9.63kg/kg/yr in each stand of C. japonica and C. obtusa respectively.

• Journal of the Korean Applied Science and Technology
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• v.40 no.2
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• pp.309-321
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• 2023

Chamaecyparis obtusa (C. obtusa) belongs to Cupressaceae and grows wild in southern area of Korea. C. obtusa has been used for furnitures because of the superior quality of the lumber and remaining branches and leaves were used for extraction of essential oil (C. obtusa oil). C. obtusa oil has numerous health effects, including anti-inflammation, antibacterial, deodorization, sedative effect. This study was performed to evaluate the effects of C. obtusa oil on anti-wrinkles, skin-barrier and moisturizing. First, DPPH and ABTS assay were conducted to evaluate antioxidant effect and C. obtusa oil showed significance scavenging activity on both radicals. And elastase activity was examined for anti-wrinkle effect. At the result, elastase activity was significantly reduced by C. obtusa oil. Next, real-time PCR was performed to evaluate gene expression. At the results, MMP-1 mRNA expression was significantly reduced by C. obtusa oil in HDF cells. In addition, filaggrin and HAS-2 mRNA expression significantly increased by C. obtusa oil in HaCaT cells. Taken together, these results indicate that C. obtusa oil has potential as a cosmetic material for anti-aging by enhancing anti-wrinkle, skin-barrier and moisturizing effects.