• Journal of Food Hygiene and Safety
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• v.19 no.4
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• pp.198-202
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• 2004

It was carried out to compare the relationship of EEC 4-plate test method and Charm II test method by HPLC. 46 only tetracyclines, 4 only sulfonamides, 4 sulfonamides+tetracyclines, one amphenicols+tetracyclines, and one amphenicols+sulfonarnides+tetracyclines, it was detected on 71 porks of EEC 4-plate positive or Charm II positive by HPLC. In case of sulfonamides and chloramphenicols, two test methods had related with each other, but had no relation in tetracyclines. Also, it was $10.5\%(6/57)$ tetracyclines, $81.8\%(9/11)$ chloramphenicols that were EEC 4 plate positive or Charm II positive, but not detectrd by HPLC. Consequently, EEC 4-plate test method and Charm II test method were suitable by screening test on antibiotics and sulfonamides etc.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.11
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• pp.1515-1522
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• 2008

Monitoring the pesticide residues in agricultural products is essential to protect consumers, obtain data for risk assessment, and ensure fair trade practices. We developed a multi-residue method for the analysis of 37 pesticides with different physico-chemical properties in agricultural products and analyzed the amount of pesticide residues on about 1,000 samples circulated in South Korea. The samples consisted of 26 different types of agricultural products selected at markets in 14 major cities; cereals (2 species), nuts (1 species) potatoes (1 species), beans (2 species), fruits (3 species), vegetables (16 species), and mushrooms (1 species). In this study, residual pesticides were detected in 23 samples (2.2%) and one sample was detected to be over maximum residue limits (MRLs, 0.1%) for pesticides in foods by the Korea food code. In leafy vegetables such as pepper leaves, radish leaves, cham-na-mul, shin-sun-cho, crown daisy, chwi-na-mul and citrus fruits such as kumquat, 8 kinds of pesticides were detected. Specially, diazinon were detected over MRLs and also, endosulfan, ethoprophos and phenthoate were detected frequently. Based on these results, we investigated the risk assesment from amount of residual pesticide, total %ADI was 1.262%, but the value has not effected on human health.

• Journal of Pharmacopuncture
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• v.17 no.1
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• pp.7-12
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• 2014

Objectives: The objective of this study is to investigate the effects of Salviae Miltiorrhizae Radix hot aqueous extract on nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) production and on 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical scavenging in macrophages. Methods: Salviae Miltiorrhizae Radix (300 g) was heated at $100^{\circ}C$ with distilled water (2 L) for 4 hours. The extract was filtered and concentrated to 100 mL by using a rotary evaporator, was frozen at $-80^{\circ}C$, and was then freeze-dried by using a freezing-drying system. The RAW 264.7 macrophage was subcultured by using $10-{\mu}g/mL$ lipopolysaccharide (LPS). In order to evaluate cytotoxicity, we performed 3-(4,5-dimrthylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays and measured the cell viability. The NO production was measured by using Griess assays, and the $PGE_2$ production was measured by using enzyme immunoassays. The antioxidant activity, the 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical scavenging capability, was measured by using the DPPH method. Results: Cell viability with the 1-, 5-, 25-, 125- and $625-{\mu}g/mL$ Salviae Miltiorrhizae Radix hot aqueous extract was not significantly decreased compared to the cell viability without the extract. When 125 and $625{\mu}g/mL$ of Salviae Miltiorrhizae Radix hot aqueous extract were used, nitric oxide (NO) production in LPS-stimulated RAW 264.7 macrophages was significantly inhibited compared to that in the control group. When 25, 125, and $625{\mu}g/mL$ of Salviae Miltiorrhizae Radix hot aqueous extract were used, $PGE_2$ production in LPS-stimulated RAW 264.7 macrophages was significantly inhibited compared to that in the control group. The 125- and $625-{\mu}g/mL$ Salviae Miltiorrhizae Radix hot aqueous extracts had high DPPH free-radical scavenging capabilities in RAW 264.7 macrophages. Conclusion: This study indicates that Salviae Miltiorrhizae Radix hot aqueous extract suppresses NO and $PGE_2$ production and improves DPPH free-radical scavenging capability. Thus, it seems that Salviae Miltiorrhizae Radix hot aqueous extract may have an anti-inflammation effect and antioxidant activity.

• Journal of Environmental Impact Assessment
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• v.25 no.2
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• pp.89-102
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• 2016

Damyang riverine wetland was designated as a wetland protected area in 2004; that is located in the Yeongsan river mainstream. Total 30 phytosociological releves at field studies were classified with 22 vegetation types including of 101 species (unidentified 1 species). Legends of actual vegetation map were separated by 6 types; riparian forest, substitute vegetation, synanthropic vegetation, wet meadow vegetation, open water, an area of wetland vegetation is about 35 % ($386,841.86m^2$). Results of this study area as follows. The plant society of Damyang riverine wetland was conjectured that it was formed by rapidly water environment change with installed weir on the upstream of protected area and operating of Damyang dam on top of the basin. Until recently, the terrace land on the river was used to cultivate, but that would be formed fallow vegetation scenery on riverfront caused by no cultivation after designated protected area. Paspalum distichum var. indutum community designated as invasive alien plant by Korea Ministry of Environment was widely developed and Myriophyllum spicatumunrecorded in the country as newly alien species was discovered in the study zone. The plants as lapped over developing environment for Leersia japonica must be occupied habitat of native plant species having similar niche. The various plant society in Damyang riverine wetland should be developed because of environmental changes, disturbances and damages of stream.

• Korean Journal of Microbiology
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• v.51 no.1
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• pp.14-20
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• 2015

Coastal plant species, Plantago camtschatica Cham. native to the coastal region of the East Sea were sampled and then morphologically different 20 endophytic fungal strains were purely isolated. Phylogenetic analysis of isolates was done by the Bayesian program based on sequenced internal transcribed spacer (ITS-rDNA) region. Culture filtrates of each of 20 isolates were treated to Waito-c rice (WR) seedlings for verifying plant growth-promoting activity, respectively. As the results, E/PC/10/1 strain showed the highest plant growth-promoting activity among them. The culture filtrate of the strain E/PC/10/1 was revealed as containing gibberellins ($GA_1$, $GA_3$, $GA_4$) by using HPLC, and gas GC/MS with selected ion monitoring (SIM). Finally, this strain was identified as novel Penicillium spinulosum species that producing new GAs with microscopic observation and further molecular analysis with beta-tubulin gene sequence.

• Journal of Environmental Impact Assessment
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• v.25 no.3
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• pp.190-208
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• 2016

This study was carried out to understand the spatial distribution and conservation, restoration plan for vegetation in the Hwaeom wetland protected area. Total 29 phytosociological releves consisted of 117 plant species (unidentified species contained) was made during field studies conducted at 2008 and 2013 applying Braun-Branquet method. The collected vegetations were arranged 8 plant communities and thus divided hydric types (Drosera rotundifolia-Eleocharis congesta community, Carex forficula-Molinia japonica community) and xeric types (Miscanthus sinensis community, Rhododendron yedoense var. poukhanense community, Tripterygium regelii community, Carex gifuensis-Quercus mongolica community) under moisture conditions. The hydric types growing in hydric conditions distributed along hollowed out ground or the water channel that have a key qualification to protect. In the wetland protected area, the hydric vegetation types were mostly covered about 3.8% and the xeric types dominating by Miscanthus sinensis were most wildly distributed about 51.5%. Various woody plant species invaded the place. In 2008, invasion plants observed with total 14 species - 8 tree species (227 individual), 6 shrub species (51 individual) and alders (Aluns japonica) found in 2013. We suggested finding out that the sustainable conservation and management in Hwaeom wetland should be setting up plan focused on activity and variability such as continuous monitoring (changing vegetation types, spatial distribution, invasion plants) and zonation management (core zone, buffer zone, transition zone).

• Food Science and Preservation
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• v.18 no.4
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• pp.590-596
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• 2011

Pimpinella brachycarpa, called as cham-na-mul in Korea, is an edible popular herb. However, the study of biological activity of P. brachycarpa is still rudimentary in worldwide. In this study, from the cultivated P. brachycarpa, we prepared the methanol extract and its subsequent solvent fractions, and their antimicrobial, antioxidation, and anti-proliferative activities were evaluated. The fraction yields of n-hexane (H), methylene chloride (EC), ethylacetate (EA), butanol (B), and water residue (W) from the methanol extract were 18.71, 0.7, 0.56, 4.57, and 71.51%, respectively. Analysis of total flavonoid and total polyphenol showed that the EA fraction contained the highest contents (89.23 and 200 mg/g), and the W residue has the lowest contents (19.6 and 2.27 mg/g) among the factions. In antimicrobial activity assay, the EA fraction showed a broad-range antibacterial activity, while the H fraction is effective against gram positive bacteria. In antioxidation activity assay, EA and B fraction showed strong DPPH anion and ABTS cation scavenging activities including reducing power, and Hand MC fraction showed effective nitrite scavenging activity (71.43~83.82 ${\mu}g$/mL of $IC_{50}$). In a while, only B fraction showed strong anti-proliferative activity against human colorectal cancer HCT-116 (166 ${\mu}g$/mL of $IC_{50}$) as a dose-dependent manner up to 200 ${\mu}g$/mL. These results suggest that the EA and B fraction of P. brachycarpa could be developed as functional food ingredients.

• Journal of Acupuncture Research
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• v.30 no.3
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• pp.101-107
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• 2013

Objectives : The purpose of this study is to investigate the correlation between acupuncture therapy on knee osteoarthritis and BMI. Methods : Data on assessment measurements of knee osteoarthritis and obesity were obtained from 31 patients suffering from knee osteoarthritis from May, 2011 to September, 2012. The assesment measurements consisted of body mass index(BMI), visual analogue scale(VAS) and Western Ontario and McMaster Universities(WOMAC) index. Statistical correlations among assessment measurements were evaluated by examining the Pearson's correlation coefficients. Results : 1. The average age of all patients was $58.87{\pm}9.48$(male : $63.67{\pm}11.34$, female : $57.72{\pm}8.86$). 2. The average BMI of all patients was $25.41{\pm}3.06kg/m^2$. According to clinical definition of obesity by Korean Society for the Study of Obesity, 25.81% of all patients was within normal weight, 25.81% was overweight, 38.71% was obesity and 9.67% was morbid obesity. 3. There were no significant correlations in statistics among assessment measurements. Conclusions : There is significant correlation between knee osteoarthritis and obesity according to many clinical and experimental researches. So more studies on correlation between knee osteoarthritis and obesity and development of assessment measurement are needed.

• The Korean Journal of Food And Nutrition
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• v.30 no.5
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• pp.1113-1118
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• 2017

The objective of this study was to investigate the non-thermal sterilization effect of methods such as high hydrostatic pressure (HHP) and UV-irradiation (specifically with regard to the storage stability and shelf-life of carrot juice. The microbial counts of the non-sterilized product increased from 5.51 to 7.34 log CFU/mL up to the fifth day, and then decreased to 5.46 log CFU/mL at six days. UV-irradiation was increased from 2.37 to 4.92 log CFU/mL. HHP was maintained under the 3 log CFU/mL. The pH of UV-irradiation and HHP was maintained withing the range of 6.29~6.30 and 6.20~6.22 during storage. However, the pH arising from non-sterilization decreased from 6.31 to 4.49. The color of non-sterilization changed significantly during storage, but UV-irradiation and HHP were similar during storage. The ${\beta}$-carotene content of non-sterilization was noted to have decreased from 269.45 to $65.19{\mu}g/mL$ during storage. The UV-irradiation and HHP decreased from 263.46 and $268.35{\sim}281.16{\mu}g/mL$ to 243.42 and $244.09{\sim}269.29{\mu}g/mL$, respectively. In conclusion, these findings suggest that HHP can be used for the pasteurization, or sterilization, of carrot juice and the optimal condition is two minutes.

• Korean Journal of Plant Taxonomy
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• v.46 no.4
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• pp.413-419
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• 2016

Two unrecorded naturalized species, Stachys agraria Schltdl. & Cham. and Dracocephalum moldavica L (Lamiaceae), are newly reported in Korea. Stachys agraria and D. moldavica were found at Seogwipo-si on Jeju-do and at ChunChen-si in Gangwondo in Korea, respectively. Stachys agraria is distinguished from other related Korean taxa by corolla of 2.4-5.2 mm long with six to twelve flowers in the node. Its local name is "Ae-gi-seok-jam-pul" based on shorter than longer corollar. Dracocephalum moldavica was distinguishable from other Dracocephalum in Korea by the following combination of characters, oblong to ovate-triangular ($1.7-2.4cm{\times}0.8-1.2cm$) leaves and rough crenate at the margin. This taxon is referred to as 'Hyang-yong-meo-ri' in Korean based on its fragrance. We provide a description, illustrations, photographs, and a key of related taxa in Korea.