• Title/Summary/Keyword: ChAT

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Distribution and Differentiation of the Choline Acetyltransferase-immunoreactive Nerve Cells in the Basal Nucleus of Meynert of the Rat Forebrains During the Postnatal Development (흰쥐 출생후 발생에 따른 전뇌 기저부 Meynert기저핵에서 Choline Acetyltranseferase 면역반응 신경세포의 분포 및 분화)

  • Lee, Nam-Seob;Chung, Young-Wha
    • Applied Microscopy
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    • v.29 no.4
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    • pp.479-491
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    • 1999
  • This study was performed to investigate the distribution and differentiation of choline acetyltransferase (ChAT)-immunoreactive cells in the basal nucleus of Meynert of the postnatal and adult rat forebrains, utilizing techniques of immunocytochemistry. According to the cell shape and the ratio of long axis vs short axis of cell soma, the ChAT-immunoreactive nerve cells in the basal nucleus of Meynert of the adult rat were classified into six types. In the adult rat, the frequency distributions (FD) of round, oval, elongated, fusiform, triangular and polygonal cells were 9.4%, 35.5%, 32.1%, 5.9%, 9.1% and 8.0%, respectively. The FD of oval and round nerve cells on the postnatal day (PND) 14 were observed to be 18.7% and 51.5%, respectively. Those were shown to be progressively decreased during developmental process to the adult. Also, those of elongated and triangular nerve cells on the PND 21 were observed to be 30.4% and 10.1%, respectively. Those were shown to be same phenomenon a,1 those in the round and oval cells. Meanwhile, those of the triangular and polygonal nerve cells were progressively increased from the early postnatal stage to the adult. The total mean volumes of ChAT-immunoreactive cell somata in the PND 7 rat were the lowest $(1,083{\mu}m^3)$ and those in the PND 21 rat were shown to be the highest $(5,045{\mu}m^3)$. But in the adult, those were decreased to $(2,731{\mu}m^3)$. Those in the PND 21 rat were shown to be about 84.7% larger than those in the adult. On the electron micrography, the cell organelles such as ribosomes, polysomes, rough endoplasmic reticula (RER) and mitochondria were well developed in the PND 21 rat forebrains, but Golgi complexes were shown to be proliferating phase. Especially, ribosomes, polysomes and RER were immunoreactive in the tissues treated with 0.05% triton X-100. According to the observations in the present study, it is considered that the ChAT-immunoreactive nerve cells in the basal nucleus of Meynert of the rat forebrains are differentiated throughout the following processes of changes during the postnatal development: 1) increase of cell soma volumes with the differentiation of tell organelles and neurites, 2) increase in the FD of differentiated tell types and 3) cell schrinkage without cell loss. The ribosomes, polysomes and RER are considered to be closely related to the intracellular localization and biosynthesis of the ChAT but not Colgi complex.

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Characteristics and Preparation of Gas Sensor Using Nano Indium Coated ZnO:In (나노 Indium을 부착한 ZnO:In 가스센서의 제작 및 특성)

  • Jung, Jong-Hun;Yu, Yun-Sik;Yu, Il
    • Korean Journal of Materials Research
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    • v.21 no.9
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    • pp.486-490
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    • 2011
  • Nano-indium-coated ZnO:In thick films were prepared by a hydrothermal method. ZnO:In gas sensors were fabricated by a screen printing method on alumina substrates. The gas sensing properties of the gas sensors were investigated for hydrocarbon gas. The effects of the indium concentration of the ZnO:In gas sensors on the structural and morphological properties were investigated by X-ray diffraction and scanning electron microscopy. XRD patterns revealed that the ZnO:In with wurtzite structure was grown with (1 0 0), (0 0 2), and (1 0 1) peaks. The quantity of In coating on the ZnO surface increased with increasing In concentration. The sensitivity of the ZnO:In sensors was measured for 5 ppm $CH_4$ gas and $CH_3CH_2CH_3$ gas at room temperature by comparing the resistance in air with that in target gases. The highest sensitivity to $CH_4$ gas and $CH_3CH_2CH_3$ gas of the ZnO:In sensors was observed at the In 6 wt%. The response and recovery times of the 6 wt% indiumcoated ZnO:In gas sensors were 19 s and 12 s, respectively.

Catalytic Reactions of 3-Phenyl-2-propen-1-ol with Perchloratocarbonylbis (triphenylphosphine) rhodium (I)$^\dag$

  • Park, Jeong-Han;Chin, Chong-Shik
    • Bulletin of the Korean Chemical Society
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    • v.8 no.4
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    • pp.324-328
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    • 1987
  • Reaction of Rh $(ClO_4)(CO)(PPh_3)_2$ (1) with trans-$C_6H_5CH = CHCH_2OH$ (2) produces a new cationic rhodium(Ⅰ) complex, $[Rh(trans-C_6H_5CH = CHCHO)(CO)(PPh_3)_2]ClO_4$ (3) where 2 is coordinated through the oxygen atom but not through the olefinic group. At room temperature under nitrogen, complex 1 catalyzes dehydrogenation, hydrogenolysis, and isomerization of 2 to give $trans-C_6H_5CH$ = CHCHO (4), trans-$C_6H_5CH = CHCH_3$ (5) and $C_6H_5CH_2CH_2CHO$ (6), respectively, and oligomerization of 2 whereas under hydrogen, complex 1 catalyzes hydrogenation of 2 to give $C_6H_5CH_2CH_2CH_2OH$ (7) and hydrogenolysis of 2 to 5 which is further hydrogenated to $C_6H_5CH_2CH_2CH_3$ (8). The dehydrogenation and hydrogenolysis of 2 with 1 suggest an interaction between the rhodium and the oxygen atom of 2, whereas the isomerization and hydrogenation of 2 with 1 indicate an interaction between the rhodium and the olefinic system of 2.

Mountain-cultivated ginseng protects against cognitive impairments in aged GPx-1 knockout mice via activation of Nrf2/ChAT/ERK signaling pathway

  • Bao Trong Nguyen;Eun-Joo Shin;Ji Hoon Jeong;Naveen Sharma;Ngoc Kim Cuong Tran;Yen Nhi Doan Nguyen;Dae-Joong Kim;Myung Bok Wie;Yi Lee;Jae Kyung Byun;Sung Kwon Ko;Seung-Yeol Nah;Hyoung-Chun Kim
    • Journal of Ginseng Research
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    • v.47 no.4
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    • pp.561-571
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    • 2023
  • Background: Escalating evidence shows that ginseng possesses an antiaging potential with cognitive enhancing activity. As mountain cultivated ginseng (MCG) is cultivated without agricultural chemicals, MCG has emerged as a popular herb medicine. However, little is known about the MCG-mediated pharmacological mechanism on brain aging. Methods: As we demonstrated that glutathione peroxidase (GPx) is important for enhancing memory function in the animal model of aging, we investigated the role of MCG as a GPx inducer using GPx-1 (a major type of GPx) knockout (KO) mice. We assessed whether MCG modulates redox and cholinergic parameters, and memory function in aged GPx-1 knockout KOmice. Results: Redox burden of aged GPx-1 KO mice was more evident than that of aged wild-type (WT) mice. Alteration of Nrf2 DNA binding activity appeared to be more evident than that of NFκB DNA binding activity in aged GPx-1 KO mice. Alteration in choline acetyltransferase (ChAT) activity was more evident than that in acetylcholine esterase activity. MCG significantly attenuated reductions in Nrf2 system and ChAT level. MCG significantly enhanced the co-localization of Nrf2-immunoreactivity and ChAT-immunoreactivity in the same cell population. Nrf2 inhibitor brusatol significantly counteracted MCG-mediated up-regulation in ChAT level and ChAT inhibition (by k252a) significantly reduced ERK phosphorylation by MCG, suggesting that MCG might require signal cascade of Nrf2/ChAT/ERK to enhance cognition. Conclusion: GPx-1 depletion might be a prerequisite for cognitive impairment in aged animals. MCG-mediated cognition enhancement might be associated with the activations of Nrf2, ChAT, and ERK signaling cascade.

A Comparative Study on the Literature of Korean and Chinese Shroud (한국수의와 중국수의와의 문화적 비교연구)

  • 유관순
    • Journal of the Korean Society of Costume
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    • v.34
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    • pp.79-89
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    • 1997
  • Comparison of Korean shroud with Chinese shroud are as follows. 1. Taetae Simeui P'oo Hansam Ko, Mal, Nukpaek, Kwatu, Ch'ungi Po-kkon Myokmok Ri Aksu Mo and Om were used the most inchina. However Mangkon Tapho Tanko Sotae Ri and Kop'o were used more widely in Korea. 2. The cloths of Chinese shroud were p'o, Paek and Kyun but those of the orean were paek Chu Chung and P'o The colors of the chinese and Koean shroud was Hyun Hun and white. 3. The size of the Cinese shroud is as follows. The size of the Ch ungi ws si-milar tothe size of jujube kernel the len-gth of Myokmok was one Ch'ok two Ch'on or one Ch'ok five Ch'on the length of Aksu was one Ch'ok two Ch'on and its width was five Ch'on. The chil of Mo reached the hands and the length of Sw-ae was three Ch'ok and the length of Om ws five Ch'on. the size of the Korean shroud was the same as Chinese shroud except that the size fo Myokmok and the lenth of Chil and Swae was seven Ch'ok respectively,. 4. In Korean and Chinese shroud Aksu was tied by the strings at two corners Myokmok was teid by the strings of four corners. The tip of the Om was divided and Mo wrapped the shole body. 5. The clothes of Soryom was nineteen Ch'ing in Korean and chinese shroud. The clothes of Taeryom in Kun were one hundred Ch'ing in the chinese and ninety Ch'ing in the Korean shroud. The imple-ment of Soryom were Kum Kyo Sangeui Saneui Ch'im Yok and Kyon in the Chinese shroud and were Kum Kyo Sangeui Saneui Ch'im Yok Kyonand Sinmyon in the Korean shroud. In the case of the implement of Taeryom the Chinese shroud had Kum Kyo Sangeui Saneui Ch'im and Yok the same as Korean shroud.

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Synthesis and Dissociation Constants of Cationic Rhodium (I)-Triphenylarsine Complexes of Unsaturated Nitriles and Aldehyde

  • Chin, Chong-Shik;Park, Jeong-Han;Shin, Sang-Young;Kim, Choong-Il
    • Bulletin of the Korean Chemical Society
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    • v.8 no.3
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    • pp.179-183
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    • 1987
  • Reactions of $Rh(ClO_4)(CO)(AsPh_3)_2$ with unsaturated nitriles and aldehyde, L, produce a series of new cationic rhodium (I) complexes, $[RhL(CO)(AsPh_3)_2]ClO_4$ (L = $CH_2$ = CHCN, $CH_2$ = C($CH_3$)CN, trans-$CH_3CH$ = CHCN, $CH_2$ = CH$CH_2$CN, trans-$C_6H_5CH$ = CHCN, and trans-$C_6H_5CH$ = CHCHD) where L are coordinated through the nitrogen and oxygen, respectively but not through the ${\pi}$-system of the olefinic group. Dissociation constants for the reaction, $[RhL(CO)(AsPh_3)_2]ClO_4$ $\rightleftharpoons$ $Rh(ClO_4)(CO)(AsPh_3)_2$ + L, have been measured to be $1.20{\times}10^{-4}$ M (L = $CH_2$ = CHCN), $1.05{\times}10^{-4}$ M (L = $CH_2$ = C($CH_3$)CN, $3.26{\times}10^{-5}$ M (L = trans-$CH_3$CH = CHCN) and $6.45{\times}10^{-5}$ M (L = $CH_2$ = CH$CH_2$CN) in chlorobenzene at $25^{\circ}C, and higher than those of triphenylphosphine complexes, $[RhL(CO)(AsPh_3)_2]ClO_4$ where L are the corresponding nitriles that are coordinated through the nitrogen atom. The differences in dissociation constants seem to be predominantly due to the differences in ${\Delta}H$ (not due to the differences in ${\Delta}S$). The weaker Rh-N (unsaturated nitriles) bonding in $AsPh_3$ complexes than in $PPh_3$ complexes (based on ${\Delta}H$ values) suggests that the unsaturated nitriles in 2∼5 are good ${\sigma}$-donor and poor ${\pi}$-acceptor.

Role of ChE and ChErs in the 21st century civilization: conceptual understanding of macroeconomic connections embedded in ChE discipline as related to the central theme (paradigm) of the 21st century civilization

  • Hyun, Jae-Chun
    • Korea-Australia Rheology Journal
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    • v.20 no.3
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    • pp.175-184
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    • 2008
  • Chemical engineering (ChE) was conceived at the close of the 19th century as a new discipline which was designed to support then the newly-emerging industries, oil and petrochemical, by supplying the competent engineers equipped with the pertinent engineering fundamentals and skills. It helped the said industries meet the various demands and ramifications of the new pattern of the human civilization spurred by the advent of automobile transportation at the turn of the 20th century. Now ChE once again is ready to fulfill its societal responsibility as probably the most important discipline and profession in sustaining the $21^{st}$ century human civilization providing the needed engineers (ChErs) and technologies. In this study, it is attempted to analyze the role of ChE and ChErs in this context, focusing on the macroeconomic connections embedded in the discipline that allow us to envision the big pictures of the 21st century civilization where the wellbeing of the mankind invariably hinges on five essential industries, i.e., medical, pharmaceutical, energy, environment and materials. It can be argued that ChE is the only discipline that can encompass simultaneously all those five industries indispensable to sustain the 21 st century human civilization that can be termed the era of "enjoy-healthy-living-longer". It is also believed that the historical mission ChE and ChErs are supposed to fulfill now is even bigger than that they took on a hundred years ago and subsequently accomplished with remarkable success in food, clothing, shelter and entertainment industries introducing various technological innovations. The macroeconomic viewpoints are called upon in this study as were in the 2006 article (Hyun, 2006) but focusing on ChE and ChErs this time to view the connections embedded in ChE as the essential components in understanding the historical nature of the role and responsibility of ChE and ChErs. The new paradigm for ChE is also pondered over together with the frequently-cited technology concepts such as IT, BT, NT, ET and ST which are regarded intimately germane to the characteristics and perspectives of the $21^{st}$ century civilization.

Capture and Reduction Technology of Greenhouse Gas Using Membrane from Anaerobic Digester Gas (분리막을 이용한 혐기성 소화가스로부터 온실가스 회수저감 기술)

  • Hwang, Cheol-Won;Jeong, Chang-Hun
    • Journal of Environmental Science International
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    • v.20 no.10
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    • pp.1233-1241
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    • 2011
  • The main objective of this experimental investigation was $CH_4$ recovery from biogas generated in municipal and wastewater treatment plant. The polysulfone hollow fiber membrane was prepared in order to investigate the permeation properties of $CH_4$ and $CO_2$. Permeability of $CO_2$ in Polysulfone membrane was 11-fold higher than of $CH_4$ gas. A membrane pilot plant for upgrading biogas was constructed and operated at a municipal wastewater treatment plant. The raw biogas contained 66 ~ 68 Vol % $CH_4$, the balance being mainly $CO_2$. The effect of the operating pressure of feed and permeate side and feed flowrate on $CH_4$ recovery concentration and efficiency were investigated with double stage membrane pilot plant. The $CH_4$ concentration in the retentate stream was raised in these tests to 93 Vol % $CH_4$.

A Gas Phaes Kinetic Study of the Energy Transfer by using the High Power CO$_2$ Laser. (II). Decomposition of $BrCH_2CH_2CH_2CH_2Cl$

  • Lee, Yong-Sik;Kim, Yang-Sik;Jeoung, Sae-Chae;Choo, Kwang-Yul
    • Bulletin of the Korean Chemical Society
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    • v.9 no.3
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    • pp.161-164
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    • 1988
  • Infrared multiphoton decompositions (IRMPD) of $BrCH_2CH_2CH_2CH_2Cl$ were studied by using the pulsed $CO_2$laser. At 0.3 J laser energy the experimentally observed product ratios could be reasonably explained by the RRKM calculation with initial excitation energy of ca. 80 Kcal/mol. The pressure dependence of product yields led us to conclude that the collisional deactivation by the inert gas decreased the yield of low energy dissociation channel more significantly.

Interaction of Di-Methylaluminum Groups with Hydroxyl Groups on a Fully Hydroxyl-Terminated Si (001) Surface

  • Kim, Dae-Hee;Kim, Dae-Hyun;Kim, Yeong-Cheol;Seo, Hwa-Il
    • Transactions on Electrical and Electronic Materials
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    • v.11 no.1
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    • pp.11-14
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    • 2010
  • The interaction of -$Al(CH_3)_2$ with -OH on a fully OH-terminated Si (001) surface was studied using density functional theory. Two sites for $Al(CH_3)_3$ to react with the -OH on the surface were identified. The $-Al(CH_3)_2$ product energetically favored the dimer-row site rather than the inter-row site because the Al atom of $-Al(CH_3)_2$ at the dimer-row site was attracted by the lone pair electrons of the O atom in the neighboring -OH. The energy barrier for the transfer of the $-Al(CH_3)_2$ between the two sites was 0.11 eV, and therefore, the $-Al(CH_3)_2$ at the inter-row site can easily transfer to the dimer-row site at room temperature.