• Journal of Acupuncture Research
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• v.19 no.5
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• pp.219-233
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• 2002

면역억제활성이 알려진 Cerves korean TEMMINCK var. manchur-icus Swinhoe(Nokyong) 약침(CPH)은 다모 뿔의 녹용을 열수추출한 용액이다. 본 연구에서는 녹용 약침의 효과를 adjuvant 유발 관절염 실험용쥐를 이용하여 골체, 골강도 및 골대체율의 감소를 평가하였다. 위의 골 대사 관련 검정실험을 위하여 6주령의 암컷 실험용쥐에 20일간 약물투여를 실시하였다. 실험적인 관절염유발은 실험용쥐의 뒤쪽 다리에 Adjuvant를 주사하여 유발시킨 결과, 요부의 골 무기질함량과 밀도(BMC, BMD) 그리고 압축강도는 관절염 실험용쥐에서 감소되었다. 10일 경과 후 골형성도(BFR/BS, BFR/BV)의 조직형태학적 기준척도인 혈청 osteocalcin 수가 정상대조군과 비교해 볼 때 현저하게 감소되었다. 그러나, 몸무게로 나눈 BMC치는 관절염 유발군과 정상군 사이에 큰 차이가 보이지 않았다. 그리고 골무기질 함량은 정상군에 비해 감소하지 않았다. 20일 경과후 몸무게로 나누거나 나누지 않은 BMC치 모두, 관절염 군에서 요부 몸체의 골무기질 함량과 강도가 정상군과 비교해 볼 때 현저하게 감소되었다. 잔존 소주(小柱)의 무기질 침착 표면은 현저하게 감소하였으며 파골 세포의 수는 증가하였다. 초기부터 매일 Shinsu(B23)에 CPH 약침 투여(10, 20, $50{\mu}g/kg$)는 20일 경과후 만성적인 다리 부종을 현저하게 방지하였으며, 골 무기질함량, 골강도 및 소주골 형성등의 감소와 파골세포수 증가도 완화하였다. Adjuvant주사로 장애를 받았던 연령대비 요부 길이 증가도 유지되었다. 이러한 결과는 Adjuvant에 의한 관절염 실험용쥐의 2차적인 골관절염에서 충분히 요추 몸체뼈와 강도 감소를 나타내기 위해서는 적어도 20일이 필요하다는 것을 제시하였다. 본 결과로부터 CPH가 실험용쥐의 관절염에 대한 골체, 골강도 및 골대체율을 조절하는데 유효하게 작용하고 있음을 알 수 있었다.

• YAKHAK HOEJI
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• v.42 no.4
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• pp.370-381
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• 1998

The effect of herbal medicine on osteoporosis was studied using ovariectomized rats as an animal model of Type I osteoporosis and SAM P6 mice as that of Type II. Each traditiona l boiling water extract of Achyranthis Radix, Psoraleae Radix, Rehmanniae Radix Preparat, Corni Fructus and Mycelia of Ganoderma, and systemic water fraction of Astragali Radix was given 5g(dried herbal weight)/kg/day p.o. for 30 days in each group of ovariectomized rats, SAM R1 and SAM P6. The extract of Cervi parvum Cornu was given for 14 days only. One ml of blood was taken by tail vein at day 0, 7, 14, 21 and 30 days after administration of the extract. Plasma levels of alkaline phosphatase, calcium, creatinine,inorganic phosphate, blood urea nitrogen, cortisol, total $T_3\;and\;total\;T_4$ were measured. In ovariectomized rats, administration of Achyranthis Radix or Corni Fructus decreased in alkaline phosphatase and that of Achyranthis Radix or Psoraleae Radix decreased in calcium comparing to the control (p<0.05). The administration of Psoralese Radix decreased in calcium and increased in urea comparing to day o(P<0.05)(Table I). There were not much changes in plasma calcium, inorganic phosphate, and alkaline phosphatase concentrations after uptake of these herbal medicine used in SAM P6(Table III). However, administration of Astragali Radix altered plasma inorganic phosphate and creatinine levels in SAM R1(p<0.01)(Table UU). The administration of Corni Fructus or Psoralease Radix induced the changes in plasma concentrations of cortisol, total $T_3$ and total $T_4$ in Type I(p<0.05) (Table IV). The uptake of Cervi parvum Cornu increased in total $T_3$ concentration and that of Mycelia of Ganodtragali Radix in SAM P6. However, the uptake of Mycelia of Ganoderma induced changes in cortisol and $T_4$ concentrations in SAM R1(p<0.05). Thus, there were significant differences in responses of herbal medicine in different types of osteoporosis.

• Journal of Acupuncture Research
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• v.27 no.4
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• pp.1-8
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• 2010

목적 : 본 연구는 녹용약침액의 주름 개선 효과에 대하여 여러 가지 in vitro 실험을 통해 확인해 보고자 계획되었다. 방법 : 녹용약침액의 항산화효과를 DPPH(1,1-diphenyl-2-picrylhydrazyl) 자유라디칼 소거법을 이용하여 측정하였고, 또, elastase 억제 효과를 측정하였다. 그리고 녹용약침액이 사람 정상 섬유아세포 HS 68에서 UVB 조사 후 type I procollagen의 생산량 회복에 미치는 효과를 ELISA법을 이용하여 측정하였다. 결과 : 녹용약침액에서 우수한 DPPH 자유라디칼 소거 효과가 관찰되었다. 또한 통계적으로 유의한 elastase 활성 억제 효과를 관찰할 수 있었다. 사람 섬유아세포 HS 68을 이용한 실험에서는 녹용약침액 처치군에서 UVB 조사로 감소된 type I procollagen이 유의하게 회복되는 것이 관찰되었다. 결론 : 본 연구 결과에 의하면, 녹용약침액에는 유효한 주름개선 효과가 있어, 미용약침 소재로 개발할 수 있을 것으로 사료된다.

• Korean Journal of Pharmacognosy
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• v.35 no.4 s.139
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• pp.276-282
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• 2004

This study has been conducted to investigate the amount of residual sulfur dioxide for herbal medicines of 30 species which are purchased in 13 different regions (Bonghwa, Busan, Chunju, Jecheun, Kwangiu, Keumsan, Seoul, Taeku, Cheungdu, Xian, Beijing, Tokyo and Osaka). The sulfur dioxide residues were determined in the collected 386 samples by the modified Monier-Williams method. The residues of sulfur dioxide in 386 samples ranged from ND (under detection limit) to 2808 ppm. The sulfur dioxide in Cassiae Semon, Cinnamomi Ramulus, Cervi Cornu, Hoelen, Crataegi Fructus, Artemisiae Argyi Herba, Scolopendrae Corpus, Schizandrae Fructus and Cyperi Rhizoma were detected under the detection limit regardless of the collected regions. The sulfur dioxide residues in herbal medicines collected in domestic regions were relatively lower than those in foreign regions. There are no standards for sulfur dioxide residual limits of herbal medicines in Korea. This results will provide the scientific basis for the standardization of sulfur dioxide residues in Korea Pharmacopoeia.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1122-1122
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• 2001

A compact and handhold near infrared (NIR) system using microspectrometer was developed. This system was suitable not only in the laboratory, but also in the field or in the process. This system was first applied for classification of geographical origin of herbal medicine such as ginseng and sesame. To identify the origin of ginseng on site, the portable NIR system is more suitable for real field application. For this study, using the compact NIR system, soft independent modeling of class analogies (SIMCA) with 1100-1750 nm NIR spectra was utilized for classification of geographical origin (Korea and China) of both ginseng and sesame. The accuracy of results is more than 90%. Quantitative analysis for petroleum such as toluene, benzene, tri-methyl benzene, and ethyl benzene was performed with partial least squares (PLS) regression with NIR 1100-1750 nm spectra. This study showed that the NIR method and gas chromatography (GC), which is a standard method, have good correlations. Furthermore, the ash content of Cornu Cervi Parvum was analyzed and the accuracy was confirmed by the developed compact NIR system.

• The Journal of Korean Medical History
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• v.33 no.1
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• pp.99-112
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• 2020

In this study, we examine the changes to Korean medicine that occurred when 'proprietary medicines' (賣藥) swept through the pharmaceutical market during the Japanese occupation (1910-1945 C.E.). Proprietary medicine during the Japanese colonial period took various forms including ready-made, over-the-counter, patent, and nostrum type pharmaceuticals. This paper examines how Korean medicine, which was the dominant form of medicine during the Joseon Dynasty, was forced to adapt to the rise of proprietary medicines. We found that the prescription of Korean medicine herbal decoctions became more like proprietary medicine in the way that they were formulated. In addition, prescriptions in Korean medicine books were reformulated with prescriptions and medicines from outside the tradition. Proprietary medicines, many of which were made with secret recipes handed down in a family, also attracted attention. Such prescriptions were made famous through advertisements and further influenced future Korean medicine doctors. New prescriptions took advantage of the trust and authority existing in traditional Korean medicine by introducing ginseng and traditional medicinal herbs such as deer antler velvet (鹿茸, Cervi Parvum Cornu). This paper argues that proprietary medicine of the Japanese colonial period distorted the concept of traditional herbal medicine.

• Journal of Oriental Neuropsychiatry
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• v.9 no.1
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• pp.59-71
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• 1998

The effect of Samyonjihwangtang(SJT) on th level of brain antioxidants was examined in aged rat. Samyongjihwangtang(SJT) is assed Cervi Pantiri-chum Cornu, Ginseng Radix to Yukmijihwangtang. The experimental groups were divided into three groups and treated as follows ; normal group(NC), Vt.E administrated group(PC), SJT administrated Group(SJT). From the purified microsome of brain tissue, those were measures the amounts of oxidant materials like malonaldehyde(MDA) and H_2O_2, then activities of antioxidants enxymes like Superoxide dismutase, Catalase, NADPH-cytochrome P-450 reductase.The results were as follows;1. In TBA reaction to measure the amount of MDA, oxidant material of brain tissue of aged rat, both treated groups showed significant decrease.2. In the formation of Hydrogen peroxide, the treated group(SJT) showed a little decrease.3. The activity of Superoxide dismutase was increased significantly in both treated groups than normal group.4. the activity of Catalase was increased significantly in both treated groups than normal group. 5. The activity of NADPH-cytochrome P-450 reductase in the treated group(SJT) showed a little increase.According to the above results, it is suggested that Samyongjihwangtag(SJT) has some antioxidant effects on the tissue of brain.

• The Journal of Pediatrics of Korean Medicine
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• v.13 no.2
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• pp.79-92
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• 1999

Background/Aims: Aplastic anemia is defined as pancytopenia (anaemia, leucopenia, and thrombocytopenia) result from aplasia of the bone marrow. Many studies have shown that survival rate of aplastic anemia is 50-60% with immunomodulation therapy. In Korea, there is a lack of research considering oriental herbal medicine with aplastic anemia. Methods: It was compared and analyzed that recently several experimental or clinical reports of oriental herbal medicine on aplastic anemia. Results and Conclusion: The oriental herb of Panax ginseng radix, Cprdonopsis pilosula radix, Astragalus membranaceus radix, Atractylodes marcrocephala. Cervi Cornu Parvum, Epimedii Herba, Boshniakiae Herba, Morindae Radix, Angelicae gigantis Radix, Cascutae Semen, Lycii Fructus, Polygoni Multiflori Radix potently stimulated hematopoietic stem cell activity, Response rate to oriental herbal medicine of aplastic anemia was 30-60% and effect rate of aplastic anemia was 73-93%, Bian zheng Lun zhi(辨證論治 treatment according to syndrome differentiation) which based on Shen xu(腎虛) is presumed to approach highest degree effect in response rate.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.13 no.1
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• pp.44-59
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• 2000

Naetakcheonkeumsan(NCS) was a drug that treated carbuncle and cellulitis. So, the purpose of this Study was to investigate effect of NCS on the anti-cancer and proliferation of lymphocytes in normal mouse group, L1210 cells-transplanted mouse group and anti-cancer drug (vincristine) 0.005mg/kg were injected mouse(Ll210 cells-transplanted) group. We used NCS extract with freeze-dried, 8wks-old male mice, and Ll210 cell lines for this Study, The proliferation of cells was tested using a colorimetric tetrazoliun assay(MTT assay). The results of this Study were obtained as follow ; Group C(NCS plus Rehmanniae Radix Preparat administered group) inhibited proliferaion of lymphocytes in normal mouse group and Ll210 cells transplanted mouse group. Group A(NCS administered group) and Group B(NCS plus Cervi pantotrichum Cornu administered group) inhibited proliferation of Ll210 cells in Ll210 cells-transplanted mouse group and anti-cancer drug were injected mouse(Ll210 cells-transplanted) group. Group C incresed proliferation of L1210 cells in L1210 cells-transplanted mouse group, but inhibited in anti-cancer drug(vincristine) 0.005mg/kg were injected mouse(L1210 cells-transplanted) group.

• Journal of Pharmacopuncture
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• v.3 no.2
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• pp.233-244
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• 2000

After observing a patient diagnosed with Progressive Muscular Dystropies from the August, 31, 2000 to the January, 2001, the following results are obtained. Method and Result: Under our assumption that the Korean Bee Venom Therapy is a good method to treat Progress Muscular Dystropies. Korean Bee Venom Therapy was applied on the following acupuncture points: BL23(Shinsu:腎兪), BL26(Guanyuanshu:關元兪), ST36(Chok-Samni:足三里), LI4(Hapkok:合谷), LV3(Taechung:太), SP10(Hyolhae:血海), SI9(Sojang-Kyonjong:貞). In addition CFC(Carthami Flos;紅花: and Cervi Pantotrichum Cornu;) Herbal-Acupuncture is also treated on the other acupuncture points. For herbal medication was given to the patient based on the Sasang Constitution, Taeyangin Ogapijangchuk-tang. Following these treatments in this case of Progressive Muscular Dystropies, the skeletal muscle functions made remarkable improvement. Conclusion: Based on the clinical results, traditional Korean Medical treatment is believed to be effective for treating Progressive Muscular Dystropies, and further studies should be conducted to provide more valuable information.