• Asian Pacific Journal of Cancer Prevention
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• v.15 no.15
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• pp.6233-6237
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• 2014

Background: Intraperitoneal spread of gynecologic cancers is a major cause of mortality and morbidity and often presents with malignant ascites. Microscopic tumor spread can be demonstrated by a peritoneal wash cytology and help assess the prognosis of the disease. In our study, the roles of paraoxonase and ceruloplasmin, measured in peritoneal washing fluid of patients operated for gynecologic pathologies in differential diagnosis was investigated. Materials and Methods: Patients operated for malign or benign gynecologic pathologies in Antalya Education and Research Hospital Gynecology Clinic between 2010-2012 were included in the study. Samples were obtained during surgery. Results: A statistically significant difference was detected between patients with benign and malign diseases with regards to PON1 levels measured in peritoneal washing fluid (p:0.044), the average values being $64.2{\pm}30.8$ (Range 10.8-187.2) and $41.4{\pm}21.4$ (Range 10.4-95.5), respectively. No significant variation was evident for ceruloplasmin. Conclusions: Paraoxonase levels measured in peritoneal washing fluid may contribute to the differentiation of malign-benign diseases in gynecologic pathologies.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.4 no.2
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• pp.199-206
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• 2001

Purpose: To review the clinical and laboratory features of patients with Wilson disease at diagnosis. Methods: In this retrospective study, records of all 20 patients, who were diagnosed as having Wilson disease at the Paik hospital in Busan from 1990 to 2000, were reviewed. Results: Out of 20 patients, 12 pateints (60%) have hepatic presentation alone, 2 patients (10%) have neurologic presentation, 4 patients (20%) have hepatic and neuropsychiatric presentation, and one patient (5%) has hematologic presentation at diagnosis. One patient (5%) has neither symptom nor laboratory finding of Wilson disease except very low serum ceruloplasmin level and positive family history. Family screening test revealed 3 cases of Wilson disease. 12 patients were revealed to be combined with liver cirrhosis at diagnosis. Conclusion: Early diagnosis and treatment is very important in patients with Wilson disease. Children or adolescents who manifest symptoms of hepatitis, who has prolonged elevation of liver enzymes, and has family history of hapatitis of unknown origin, with mild hematologic or urinary abnormalities must be suspected to have Wilson disease. Also, in adolescents with extrapyramidal symptoms or other neuropsychiatric symptoms, liver function test should be done.

• Proceedings of the KSCN Conference
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• 1996.12a
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• pp.36-36
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• 1996

• Korean Journal of Community Nutrition
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• v.2 no.1
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• pp.105-122
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• 1997

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.124-124
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• 1993

녹용은 강심작용, 여성홀몬작용, 강장작용, 창상치유작용 등의 약리작용이 있는 것으로 보고되어 있는데 녹용의 상용량에서는 강장작용, 창상치유작용이 있는 것으로 알려져 있다. 녹용의 창상치유작용은 생체내 질소 평형이 음의 방향으로 되었을 때 이를 빠른 시간내에 정상적으로 회복시키는 약리효과에 기인하는 것으로 볼 수 있겠다. 본 연구에서도 흰쥐에 turpentineee oil (TPO)를 주사하여 acute-phase response를 일으켜 질소평형을 깨뜨린 실험 모델에서 녹용의 효과를 측정하였다. Acute-phase reactants중에서 $\alpha$$_2$-macroglobulin, $\alpha$$_1$-cysteine pretense inhibitor (T-kininogen), ceruloplasmin을 측정 하였는데 이 중에서 creuloplasmin의 측정이 재현성이 높았다. TPO로 급성염증을 유발시키면 2일 후에 ceruloplasmin의 혈청내 농도가 약 100% 증가하며 4일까지 지속하다가 5일째부터 감소하는데 이때 미리 녹용추출물을 투여한 군은 TPO주사 후 4일째에 50% 감소하였다. TPO로 염증을 유발하기 전에 녹용을 투여했을 때가 염증유발 후에 투여했을 때보다 녹용의 효과가 현저히 나타났다. 이러한 결과는 급성염증에 의해 깨어진 단백질 평형을 신속히 정상화시키는 효과가 있음을 나타내는 것으로 이러한 녹용의 효과를 지표로 하여 녹용의 유효성분 분리를 수행하고자 한다.

• Korean Journal of Community Nutrition
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• v.7 no.6
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• pp.844-851
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• 2002

Smoking can increase oxidative stress and thereby change the antioxidant defense system in the body. To investigate the relationship between male adolescent smoking and antioxidant status, we surveyed the eating habits and dietary intake of 82 smokers and 44 nonsmokers recruited from a male technical high school. In addition, antioxidant enzyme activity and lipid peroxide values were determined in both the plasma and the erythrocytes. Although the frequency of food intake was not significantly different, most nutrient intake was unexpectedly higher in smokers than in nonsmokers. In comparison with the Korean RDA, especially the average intake of Ca, Fe and vitamin $B_2$ didn t reach 75% of the Korean RDA in either smokers or nonsmokers. An analysis of antioxidant enzyme activity showed that plasma catalase. superoxide dismutase (SOD), glutathione peroxidase (GSH-px), erythrocyte catalase and GSH-px activities showed no significant difference between smokers and nonsmokers. However, the erythrocyte SOD activity of smokers (1.57 unit/mgHb) was significantly lower than that of nonsmokers (2.00 unit/mg Hb). In addition, the plasma ceruloplasmin concentration of smokers (28.68 mg/$d\ell$) was significantly higher than that of nonsmokers (26.30 mg/$d\ell$), whereas the specific ceruloplasmin ferroxidase activity of smokers (0.31 unit/mg) was lower than that of nonsmokers (0.35 unit/mg). The plasma and erythrocyte thlobarbituric acid reactive substance (TBARS) of smokers (2.57 $\mu$mol/L, 0.32 $\mu$mol/gHb) were also significantly higher than those of nonsmokers (2.25 $\mu$mol/L, 0.27 $\mu$mol/gHb). The overall data indicate that adolescent smoking might decrease the antioxidant capacity of the body, in part, by lowering the erythrocyte SOD activity and the specific ceruloplasmin ferroxidase activity.

• Parasites, Hosts and Diseases
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• v.49 no.2
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• pp.133-138
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• 2011

This study was undertaken to investigate the hematological and biochemical changes in experimentally infected goats with Besnoitia caprae from the time of infection till 360 days post-infection (PI). Six male goats were inoculated subcutaneously with $13{\times}10^7$ bradyzoites of B. caprae, and blood samples were collected from the jugular vein. The total erythrocyte and total leukocyte counts, hematocrit value, and differential leukocyte counts were determined. Serum biochemical analysis, including the total protein, albumin, total globulin, cholesterol, triglyceride, chloride, testosterone, calcium ($Ca^{2+}$), inorganic phosphorus, sodium ($Na^+$), potassium ($K^+$), iron ($Fe^{2+}$), glucose, serum amyloid A (SAA), haptoglobin (Hp), fibrinogen, ceruloplasmin, aspartate aminotransferase, alanine aminotransferase, creatine kinase, lactate dehydrogenase, and alkaline phosphatase, was undertaken. Skin biopsy from the limbs were collected at weekly intervals and histologically examined for Besnoitia cysts. Cysts were present in the skin biopsies of the leg of the infected goats from day 28 PI. There were variations in hematological analyses, but no significant difference was seen. From day 30 to 360 PI, results showed that SAA, Hp, fibrinogen, and ceruloplasmin concentrations increased, whereas testosterone concentrations decreased. Infected goats exhibited decrease of albumin and increase of serum total protein and globulin concentrations. By contrast, there were no significant differences in the remained analyses concentrations.

• Biomolecules & Therapeutics
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• v.22 no.1
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• pp.47-54
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• 2014

The present study was aimed to evaluate the antioxidant defense system of cinnamaldehyde in normal, diabetic rats and its possible protection of pancreatic ${\beta}$-cells against its gradual loss under diabetic conditions. In vitro free radical scavenging effect of cinnamaldehyde was determined using DPPH (1,1-diphenyl-2-dipicrylhydrazyl), superoxide radical, and nitric oxide radical. Streptozotocin (STZ) diabetic rats were orally administered with cinnamaldehyde at concentrations of 5, 10 and 20 mg/kg body weight for 45 days. At the end of the experiment, the levels of plasma lipid peroxides and antioxidants such as vitamin C, vitamin E, ceruloplasmin, catalase, superoxide dismutase, reduced glutathione and glutathione peroxidase were determined. A significant increase in the levels of plasma glucose, vitamin E, ceruloplasmin, and lipid peroxides and significant decrease in the levels of plasma insulin and reduced glutathione were observed in the diabetic rats. Also the activities of pancreatic antioxidant enzymes were altered in the STZ-induced diabetic rats. The altered enzyme activities were reverted to near-normal levels after treatment with cinnamaldehyde and glibenclamide. Histopathological studies also revealed a protective effect of cinnamaldehyde on pancreatic ${\beta}$-cells. Cinnamaldehyde enhances the antioxidant defense against reactive oxygen species produced under hyperglycemic conditions and thus protects pancreatic ${\beta}$-cells against their loss and exhibits antidiabetic properties.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.89-90
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• 2003

Effects of interaction of dietary soybean oil with NaHCO$_3$on the energy metabolism and antioxidants enzyme activity in broiler chicks during acute phase response was studied, Broiler chicks 10 d-old were fed on diets containing 5.0 ％ of soybean oil with or without 0.5 ％(60 meq/kg) of NaHCO$_3$. Then acute phase response were induced by injecting Salmonella typhymurium lipopolysacharide(LPS) In saline 3 times i.p. at alternative day on 16-d of age. Dietary soybean oil with NaHCO$_3$lessened the performance(growth, feed efficiency, calcium balance, excretion of uric acid, and nitrogen balance) -suppressing effect of the LPS effect, but enhanced metabolizable energy value of diet due to increased digestive absorption of energy sources and phosphoruse balance, and decreased activities of SOD in erythrocyte cytosols and circulating ceruloplasmin in plasma. The results indicated dietary energy utilization and electrolytes levels may interact with the antioxidants systems during acute phase response.

• BMB Reports
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• v.38 no.1
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• pp.71-76
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• 2005

Ceruloplasmin (CP) is the major plasma antioxidant and copper transport protein. Monoclonal antibodies (mAbs) against human CP were produced and characterized. A total of five hybridoma cell lines were established (CP2, CP10, CP20, CP25, CP30). From the epitope mapping analysis, two subgroups of mAbs recognize different peptide fragments were identified. When the purified CP was incubated with the mAbs, the ferroxidase activity of CP was inhibited up to a maximum 57%. Immunoblotting with various tissue homogenates indicated that all the mAbs specifically recognize a single protein band of 130 kDa. They also appear to be extensively cross-reactive among different mammalian including human and avian sources. These results demonstrated that only one type of immunologically similar CP is present in all of the mammalian tissues including human. The CP mAbs could be of great benefit to design the diagnostic kit for CP-related diseases such as Wilson's disease.