• 약학회지
• /
• 제36권3호
• /
• pp.230-240
• /
• 1992

The metabolic profile of doxylamine, N,N-dimethyl-2-[1-phenyl-1-(2-pyridinyl)ethoxy] ethanamine, was determined in the human urine. The free fractions of extracts were obtained without hydrolysis, and the conjugated fractions of extracts were obtained with enzyme hydrolysis using ${\beta}-glucuronidase/arylsulfatase$ from Helix pomatia. The mixture of acetic anhydride/pyridine (10 : 1, v : v) was used to derivatize the urinary extracts and then analyzed by gas chromatography and mass selective detector. N-desmethyldoxylamine, doxylamine carboxylic acid, desaminohydroxydoxylamine, N, N-didesmethyldoxylamine, N-acetyl conjugates of N-desmethyl and N, N-didesmethyldoxylamine, quarternary ammonium N-glucuronide of doxylamine, N-desmethyldoxylamine N-glucuronide and unchanged doxylamine were detected in the human urine obtained after oral treatment with doxylamine succinate. $N-methyl-{\alpha}-hydroxy-2-[1-phenyl-1-(2-pyridinyl)$ ethoxy] ethanamine, which can be a key intermediate of this metabolism, was tentatively identified by the interpretation of its mass spectrum. In this study, we proposed the metabolic pathway of doxylamine in the human on the basis of our data of the identified metabolites of doxylamine.

• Mass Spectrometry Letters
• /
• 제9권1호
• /
• pp.30-36
• /
• 2018

A quantitation method for free amino acids in human serum was developed using a stepwise-dilution method and a bimodal cation exchange (CEX)/hydrophilic interaction liquid chromatography (HILIC)-tandem mass spectrometry system equipped with an electrospray ionization source (ESI/MS/MS). This method, which was validated using quality control samples, was optimized for enhanced selectivity and sensitivity. Dithiothreitol (DTT) was used as a reducing agent to prevent the oxidation of a serum sample ($50{\mu}L$), which was then subjected to stepwise dilution using 3, 30, and 90 volumes of acetonitrile containing 0.1% formic acid. Chromatographic separation was performed on an Imtakt Intrada Amino Acid column ($50mm{\times}3mm$, $3{\mu}m$) in mixed mode packed with CEX and HILIC ligands embedded in the stationary phase. Underivatized free amino acids were eluted and separated within 10 min. As a result of the validation, the precision and accuracy for the inter- and intraday assays were determined as 2.11-11.51% and 92.82-109.40%, respectively. The lowest limit of quantification (LLOQ) was $0.5-4.0{\mu}g/mL$ and the matrix effect was 80.22-115.93%. The proposed method was successfully applied to the quantitative analysis of free amino acids in human serum.

• Biomolecules & Therapeutics
• /
• 제4권3호
• /
• pp.251-256
• /
• 1996

The metabolism of carbinoxamine, 2-[(4-chlorophenyl)-2-pyridinyl-methoxy]-N, N-dimethylethaneamine, was studied in adult male volunteers after an oral dose of 15 mg. Solvent extracts of urine obtained with or without enzyme hydrolysis were analyzed by gas chromatography-mass spectrometry after derivatization with MSTFA/TMSCl (N-methyl-N-trimethylsilyltrifluoroacetamide/trimethyl chlorosilane). The structures of metabolites were determined based on the electron impact (EI) and chemical ionization (CI) mass spectra. Nonconjugated metabolites identified in the urine were carbinoxamine, nor-carbinoxamine, and bits-nor-carbinoxamine. Parent drug, nor-carbinoxamine, and bits-nor-carbinoxamine were also detected as conjugated forms. These metabolites observed in human urine were different from those previously reported in the rat. Urinary excretions of carbinoxamine were reached to maxima in 4 hours after drug administration with 4.9%-8.1% and 2.5-4.2% of the dose excreted during 24 h as carbinoxamine and its glucuronide, respectively.

• 해양환경안전학회지
• /
• 제28권6호
• /
• pp.937-945
• /
• 2022

자율운항선박(MASS : Maritime Autonomous Surface Ships)은, 고도의 자율도를 가지고, 계획된 경로를 따라 자율 운항하지만, 필요시 육상원격제어센터(SRCC : Shore Remote Control Center)에서 선박의 운항에 직접 개입할 수 있다. 본 연구에서는 이러한 자율운항선박의 운항을 육상에서 모니터링하고 유사시 원격제어하는 역할을 담당할 육상원격제어사(SRCO : Shore Remote Control Officer)의 교육훈련에 필요한 시뮬레이터 시스템의 운용개념과 이를 가능하게 하기 위한 요구기능에 대해 검토하였다. 육상원격제어 시뮬레이터 시스템은, 다수의 자율운항선박의 운항상황을 모니터링하는 Monitoring Station, 유사시 특정 선박의 운항에 직접 원격개입하는 Control Station의 기능을 모의하도록 하였고, 시뮬레이션 종합통제실, 자율운항선박 운항상황 모의 시뮬레이터, 그리고 주변의 유인선 운항을 모의하기 위한 통항선 시뮬레이터 등으로 구성하였다. 기능적으로는, 육상에서 선박을 직접 제어하기 위하여 원격으로 개입하는 ESRC(Emergency Situation for Remote Control) 상황을 정의하여 이러한 상황을 모의할 수 있도록 하였다.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2001년도 Proceedings of the Pharmaceutical Society of Korea
• /
• pp.221.2-222
• /
• 2001

• 대한화학회지
• /
• 제33권3호
• /
• pp.332-334
• /
• 1989

• Mass Spectrometry Letters
• /
• 제7권3호
• /
• pp.55-63
• /
• 2016

Growth hormone (GH)-releasing peptides (GHRPs) and GH secretagogues (GHSs) are listed in the World Anti-Doping Agency (WADA) Prohibited List. In the present study, we developed and validated a method for the simultaneous analysis of seven GHRPs (alexamorelin, GHRP-1, -2, -4, -5, -6, and hexarelin) and three GHSs (anamorelin, ibutamoren, and ipamorelin) in human urine. Method validation was performed at minimum required performance levels specified by WADA technical documents (2 ng/mL) for all substances, and the method was validated with regard to selectivity (no interference), linearity (R2 > 0.9986), matrix effects (50.0%-141.2%), recovery (10.4%-100.8%), and intra- (2.8%-16.5%) and inter-day (7.0%-22.6%) precisions. The limits of detection for screening and confirmation were 0.05-0.5 ng/mL and 0.05-1 ng/mL, respectively.

• Archives of Pharmacal Research
• /
• 제16권3호
• /
• pp.213-218
• /
• 1993

($\pm$)-Higenamine is known as a cardiotonic principle of aconite root (root of Aconitum spp., Ranunculaceae). A simple and sensitive detection method for higenamine was developed by using gas chromatography-mass spectrometry (GC/MS). The recovery of higenamine after extraction and concentration with XAD-2 resin column was around 95% from rat biological fluids such as bile, plasma and urine. The limits of detection of higenamine in these biological fluids were approximately 0.1 ng/ml each. It has well been suggested that tetrahydroisoquinolines possessing catechol moiety such as higenamine should be subjected to the catechol-O-methyl transferase (COMT) activity in vivo. We detected two major peaks of presumed metabolites of higenamine in the total ion chromatogram obtained from the rat urine sample after the oral adminstration of ($\pm$)-higenamine. The scan mass spectrum of one of the metabolties coincided with those obtained from coclaurine $(C_6$-O-methyl higenamine) and those of the other metabolite are suggestive of isococlaurine $(C_7$-O-methyl higenamine).

• 한국우주과학회:학술대회논문집(한국우주과학회보)
• /
• 한국우주과학회 2008년도 한국우주과학회보 제17권2호
• /
• pp.35.3-36
• /
• 2008

The ground-based spacecraft simulator is a useful tool to realize various space missions and satellite formation flying in the future. Also, the spacecraft simulator can be used to develop and verify new control laws required by modern spacecraft applications. In this research, therefore, Hardware-in-the-loop (HIL) simulator which can be demonstrated the experimental validation of the theoretical results is designed and developed. The main components of the HIL simulator which we focused on are the thruster system to attitude control and automatic mass-balancing for elimination of gravity torques. To control the attitude of the spacecraft simulator, 8 thrusters which using the cold gas (N2) are aligned with roll, pitch and yaw axis. Also Linear actuators are applied to the HIL simulator for automatic mass balancing system to compensate for the center of mass offset from the center of rotation. Addition to the thruster control system and Linear actuators, the HIL simulator for spacecraft attitude control includes an embedded computer (Onboard PC) for simulator system control, Host PC for simulator health monitoring, command and post analysis, wireless adapter for wireless network, rate gyro sensor to measure 3-axis attitude of the simulator, inclinometer to measure horizontality and battery sets to independently supply power only for the simulator. Finally, we present some experimental results from the application of the controller on the spacecraft simulator.

• Parasites, Hosts and Diseases
• /
• 제59권6호
• /
• pp.615-623
• /
• 2021

Human sparganosis is a food-borne parasitic disease caused by the plerocercoids of Spirometra species. Clinical diagnosis of sparganosis is crucial for effective treatment, thus it is important to identify sensitive and specific antigens of plerocercoids. The aim of the current study was to identify and characterize the immunogenic proteins of Spirometra erinaceieuropaei plerocercoids that were recognized by patient sera. Crude soluble extract of the plerocercoids were separated using 2-dimensional gel electrophoresis coupled with immunoblot and mass spectrometry analysis. Based on immunoblotting patterns and mass spectrometry results, 8 antigenic proteins were identified from the plerocercoid. Among the proteins, cysteine protease protein might be developed as an antigen for diagnosis of sparganosis.