• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.40-40
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• 2018

비고사리과(Lindsaeaceae) 버들일엽[Laxqgramme saliciblia (Makino) Makino]은 잎이 녹색의 단엽으로 버들처럼 보여 관상가치가 높아 조경소재로 이용되는 착생식물이다. 또한 식물구계학적 특정식물종 V급, 적색자료집 VU급, 국외반출 승인대상종에 수록된 식물로 생태학적 보전이 요구되며, 개체수도 적은 편으로 알려져 있다. 본 연구는 보전이 요구되는 버들일엽의 전엽체 증식 및 포자체 형성을 위한 기내 외 번식조건을 구명하고자 수행되었다. 서귀포시 상효동 일대에서 식물을 획득하였으며, 기내에서 포자를 발아시켜 획득한 전엽체를 8주 간격으로 계대배양하면서 실험재료를 확보하였다. 전엽체 증식에 적합한 배양배지를 선발하고자, 전엽체 300mg을 다진 후 배지종류와 농도를 달리한 배지(Knop, 1/4, 1/2 및 1MS)에 8주간 배양하였다. 배양실은 온도 $25{\pm}1.0^{\circ}C$, 광도 $30{\pm}1.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$(16/8h)로 조절되었다. 연구의 결과, 1/2MS배지에서 전엽체의 생체중이 5.8g으로 가장 많이 증가하였으며, 형태형성의 발달도 주걱형의 전엽체로 정상적으로 발달하였다. 기외 포자체 형성에 적합한 토양조건을 확인하고자, 인공토양(원예상토, 피트모스, 펄라이트 및 마사토)의 비율을 달리하여 5종류의 혼합토양을 조성하였다. 준비된 토양을 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진하고, 전엽체 1g을 10초간 분쇄한 다음 토양표면에 분주하여 16주간 재배하였다. 재배환경은 온도 $25{\pm}1.0^{\circ}C$, 광도 $43{\pm}2.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$(16/8h) 및 습도 $72{\pm}2.0%$로 조절되었다. 연구의 결과, 원예상토가 많은 비율로 혼합된 처리에서 포자체의 형성이 확인되었다. 원예상토와 펄라이트를 2:1(v:v)로 혼용한 토양, 원예상토와 마사토를 2:1(v:v)로 혼용한 토양, 원예상토 단용한 토양에서 각 28.5, 23.0, 22.3개로 조사되었다.

• 천문학회보
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• 제42권2호
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• pp.81.1-81.1
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• 2017

L1251B is an excellent example of a small group of pre- and protostellar objects in low-mass star-forming region. Previous interferometer data with a high angular resolution resolved the outflows associated with L1251B into a few components. To understand the physical conditions of the protostellar outflows in L1251B, we mapped this region spectroscopically with Spitzer/IRS and obtained spectral line data from Herschel/PACS. Spitzer/IRS provides the S(0)-S(7) pure rotational lines of H2 as well as fine-structure emissions produced in shocks such as S, [Si II], and [Fe II] and it is a powerful tool for studying shocked interstellar gas. In addition, [O I] lines observed with Herschel/PACS are described well by J-type shock models expected in the outflows from protostars. We will present an analysis of the L1251B protostellar outflow in the H2 pure rotational lines and fine-structure emissions.

• 동력기계공학회지
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• 제12권1호
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• pp.41-46
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• 2008

The primary objective of this study is to truly understand exciting forces of the in-line 4 cylinders engine. Exciting forces of the engine apply a source of the vehicle NVH(Noise, Vibration, Harshness). To understand exciting forces, first was governed theoretical equations for single cylinder engine. And this theoretical equations was programming using MATLAB software. To compare theoretical analysis value, was applied MSC.ADAMS software. To determined the specification of engine(2,000cc, in-line 4) was applied ADAMS/Engine module. And this specification for engine was applied ADAMS/View and MATLAB software. The geometry model for ADAMS/View analysis was produced by the 3-D design modeling software. After imported 3-D model, each rigid body was jointed suitable. Under idle speed for engine, was analysed. The results of analysis are fairly well agreed with those of three analysis method. Using MATLAB software proposed in this study, engine exciting fores can be predicted. Also using ADAMS/Engine module and ADAMS/View software, engine exciting forces can be predicted.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회B
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• pp.1984-1989
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• 2008

In this study, fire simulations were performed to analyze the characteristics of the fire driven flow and the effects of the platform screen door on the smoke flow in the station, when the fire occurred in the center of the platform. Soongsil Univ. station (line number 7, 47m in depth underground) was chosen which was the one of the deepest underground subway stations in the Seoul metro, SMRT. The parallel computational method was employed to compute the heat and mass transfer eqn's with 6 CPUs of the linux clustering machine. The fire driven flow was simulated with using FDS code in which LES method was applied. The Heat release rate was 10MW and The Ultrafast model was applied for the growing model of the fire source. The 10,000,000 structured grids were used.

• 대한영양사협회학술지
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• 제29권2호
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• pp.100-113
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• 2023

This was a clinical trial study to aid the recovery of elderly female alcoholic liver disease patients by providing a balanced nutritional supplement comprising soymilk. All patients gave their consent before enrolling. The average demographics of the subjects were age 81.57 years, height 150.43 cm, weight 52.67 kg, and body mass index 24.15 kg/m². An increase in the daily consumption of fruits and fruit juice was observed after the patients had started taking the balanced meal as compared to before. Intakes of vitamin A, vitamin C, vitamin E, and cholesterol decreased after consuming soymilk, whereas vitamin B₁₂, niacin, folic acid, and dietary fiber significantly increased (P<0.05). Blood cholesterol and BUN levels showed a decreasing tendency. Our results indicate that consuming soymilk in a balanced diet for female patients afflicted with alcoholic liver disease helps improve their nutritional status by increasing the nutrients lacking in the body.

• 한국식품영양학회지
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• 제27권3호
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• pp.470-477
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• 2014

The purpose of this study was to investigate the association of homocysteine and antioxidant nutrients with obesity in Korean middle aged women. The study subjects included were middle-aged obese women (n=36) whose body mass index (BMI) was greater than $25kg/m^2$. A total of 36 participants were randomly divided into two groups. They were assigned to either the Nutritional Management and Exercise (NME) program or the Exercise Management (EM) program, which were both conducted for 3 months. We measured serum homocysteine, total cholesterol and HDL cholesterol levels of the participant. We also measured the dietary intakes of antioxidant nutrient, cholesterol, body weight, body mass index, blood pressure, and $VO_2$. At the end of both programs, please confirm this change. It was unclear when these measurements were shown to have decreased. The body weight and BMI of NME group were decreased compared to those of EM group. The serum total cholesterol levels of the participants in NME group were significantly decreased, in contrast to EM group. Also, serum homocysteine levels of NME group participants were decreased compared to EM group, but the difference was not statistically significant. In the NME group, there were significant differences in the change of dietary cholesterol and vitamin C intake. These results showed that participating NME program will continuously promote the healthy status of the middle aged obese women, compared to participating only in the EM program.

• 한국산림과학회지
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• 제76권2호
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• pp.161-168
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• 1987

임업기계훈련원(林業機械訓練院)에서 1984년(年) 시범적(示範的)로 임도(林道)를 시설(施設)(10 km)할 목적(目的)으로 10 km를 시공(施工)했는데 1986년(年) 태풍(颱風) 내습시 부분적(部分的)으로 노견붕괴(路肩崩壞)와 측구침식(側溝浸蝕)이 발생(發生)하였다. 이에 대한 원인(原因)을 조사분석(調査分析)한 결과(結果)는 다음과 같다. 측구침식(側溝浸蝕)에 의한 피해(被害)길이는 전림도(全林道) 연장(10km)의 3%에 해당하였으며, 대부분(大部分) 요형사면(凹型斜面)에 사면장(斜面長) 10m 이상(以上)으로 성토(盛土)된 곡선부(曲線部)에서 발생하였고, 부분적(部分的)으로 상수(常水)가 흐르는 계곡부(溪谷部)에 성토(盛土)된 구역(區域)과 상수(常水)가 있는 배수관(排水管)의 유출구(流出口) 양사면(兩斜面)에 성토(盛土)된 부위에서 붕괴(崩壞)가 많이 발생하였다. 측구침식(側溝浸蝕)의 경우 심한 피해(被害)는 계곡수(溪谷水)가 월수(越水)하여 V형(型) 측구(側溝)를 따라 흐르므로서 피해(被害)를 발생시켰으며, 기타 원인(原因)으로는 종단(縱斷)물매가 10% 이상(以上) 급(急)하게 시공(施工)된 지역(地域)에서 나타나 문제점(問題點)으로 인식되고, 직경(直經) 400mm 이하(以下)인 배수관(排水管)이 매설된 집수정(集水井)이 낙엽(落葉) 등(等)의 퇴적(堆積)으로 매몰된 경우와 경사가 급한 침사지(沈砂池)(집수승(集水桝)) 벽이 무너져 내린 결과 이 지점을 월수(越水)하여 측구침식(側溝浸蝕)을 일으키고 있다. 이상(以上)의 결과(結果)에서 시범임도(示範林道)의 태풍피해(颱風被害)는 임도피해예방(林道被害豫防)을 위한 경험부족(經驗不足)에서 나타낸 현상으로 보인다. 상기(上記)와 같은 점을 개선하면 동(同) 시범임도(示範林道) 조성방법(造成方法)은 경제적(經濟的) 임도(林道)차 모델로서 발달될 수 있을 것이다.

• Archives of Pharmacal Research
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• 제28권9호
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• pp.1086-1091
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• 2005

An analytical method was developed for evaluating the cannabidiol (CBO), cannabinol (CBN), ${\Delta}^9-tetrahydrocannabinol$ $({\Delta}^9-THC)$ level in human hair using gas chromatography-mass spectrometry (GC-MS). Hair samples (50mg) were washed with isopropyl alcohol and cut into small fragments (< 1mm). After adding a deuterated internal standard, the hair samples were incubated in 1.0M NaOH for 10 min at $95^{\circ}C$. The analytes from the resulting hydrolyzed samples were extracted using a mixture of n-hexane-ethyl acetate (75:25, v/v). The extracts were then evaporated, derivatized, and injected into the GC-MS. The recovery ranges of CBD, CBN, and ${\Delta}^9-THC$ at three concentration levels were $37.9-94.5\%$ with good correlation coefficients $(r^2>0.9989)$. The intra-day precision and accuracy ranged from $-9.4\%\;to\;17.7\%$, and the inter-day precision and accuracy ranged from $-15.5\%\;to\;14.5\%$, respectively. The limits of detection (LOD) for CBD, CBN, and ${\Delta}^9-THC$ were 0.005, 0.002, and 0.006 ng/mg, respectively. The applicability of this method of analyzing the hair samples from cannabis abusers was demonstrated.

• Fisheries and Aquatic Sciences
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• 제21권3호
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• pp.5.1-5.8
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• 2018

Stimulator of interferon gene (STING) is induced by various inflammatory agents, such as lipopolysaccharide and microbial pathogens, including virus and bacteria. In this study, we obtained a full-length cDNA of a STING homolog from olive flounder using rapid amplification of cDNA ends PCR technique. The full-length cDNA of Paralichthys olivaceus STING (PoSTING) was 1442 bp in length and contained a 1209-bp open reading frame that translated into 402 amino acids. The theoretical molecular mass of the predicted protein sequence was 45.09 kDa. In the PoSTING protein, three transmembrane domains and the STING superfamily domain were identified as characteristic features. Quantitative real-time PCR revealed that PoSTING expressed in all the tissues analyzed, but showed the highest level in the spleen. Temporal expression analysis examined the significantly upregulated expression of PoSTING mRNA after viral hemorrhagic septicemia virus (VHSV) stimulation. In contrast, no significant changes in the PoSTING expression were detected in Edwardsiella tarda-challenged group compared to the un-injected control. The expression of P. olivaceus type I interferon (PoIFN-I) was also highly upregulated upon VHSV challenge. These results suggest that STING might be involved in the essential immune defense against viral infection together with the activation of IFN-I in olive flounder.

• Asian-Australasian Journal of Animal Sciences
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• 제23권9호
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• pp.1236-1243
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• 2010

The aim of this study was to screen lactic acid bacteria for the fermentation of garlic and to assess the increase in inhibitory activity of garlic fermented against antibiotic-resistant pathogens for use as an animal feed supplement. We screened 45 strains of lactobacillus for the fermentation of garlic. Of these strains, 23 showed similar growth rates with or without allicin. Cultures of the 23 strains were mixed with an equivalent amount of garlic juice and incubated overnight at $37^{\circ}C$. The three strains with the lowest pH values were Lactobacillus paracasei KCTC 3169, L5 strain, and L. reuteri SW. Garlic juice fermented by the L5 strain more strongly inhibited antibiotic-resistant pathogenic bacteria than L. paracasei KCTC 3169, L. reuteri SW, or garlic juice itself. By examining carbohydrate utilization, morphologic properties and 16S rRNA gene sequences, we identified the L5 strain as Pediococcus pentosaceus and deposited it in the name of P. pentosaceus KACC 91419 into the Korea Agricultural Culture Collection. To identify the antimicrobial compound from the garlic filtrate fermented by P. pentosaceus KACC 91419, we fractionated P. pentosaceus KACC 91419 culture on a C18 column and checked the antimicrobial activity of fractions A6 to A10. Only fraction A9 showed inhibitory activity on Staphylococcus aureus. Comparing the mass spectra of the fractions with and without antimicrobial activity, we observed a single dominant product ion (m/z 157.99) from the fraction showing antimicrobial activity. Its molecular mass (157.99) was 2 atomic mass units less than that of allicin (162.02). This suggests that allicin might be converted to its derivative, which has antimicrobial activity, during fermentation by P. pentosaceus KACC 91419.