• Asian Journal of Turfgrass Science
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• v.23 no.2
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• pp.361-368
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• 2009

Cenangium ferruginosum was known as the causal agent of dieback of pines including Pinus koraiensis and Pinus densiflora. Since the first report of the disease in Korea in 1989, a group dying occurred in Seoul, Gyeonggi, Kangwon and Chungbuk in 2007 spring. Although C. ferreginosum was known as a weak pathogen or a parasite, this disease caused in stressed pine by drought, wounding, extremely cold weather or unusual warm winter. In this study, we explained the features of cenangium dieback with the characteristics of pathogen to understand the trend of disease associated with the climatic change of the world. We collected pycnidia and apothecia from the diseased branches and stems of P. koraiensis and P. densiflora in Gyeonggi, Chungcheong and Gyeongsang province to characterization of pathogen. The fungal development on the diseased branches were observed and the isolates from pycnidia and apothecia were identified as Cenangium ferruginosum by their morphological characteristics and the molecular techniques.

• Journal of Korean Society of Forest Science
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• v.87 no.4
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• pp.557-561
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• 1998

A die-back fungus isolated from Pinus koraiensis was identified as Cenangium ferruginosum. Mycelial growth of the fungus was the best on PDA among five media tested. Mycelial growths on agar media supplemented with extracts from coniferous trees were relatively better than those on media with extracts from non-coniferous trees. Optimum temperature and pH for the mycelial growth of C. ferruginosum on PDA were $10^{\circ}C$ and pH 5, respectively. When five-years-old P. koraiensis seedlings were artificially inoculated with the fungus for the pathogenicity test of this fungus, 80% of seedlings were infected. and the same symptoms and signs were developed as those of naturally-infected trees.

• The Plant Pathology Journal
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• v.17 no.4
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• pp.216-221
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• 2001

Dieback of pine branches or twigs with brown needles occurs most commonly on Pinus species after severe winter in Korea. In this study, Cenangium ferruginosum was isolated from infected stems, branches, and twigs of Pinus koraiensis (C1), P. densiflora (C2), and P. thunbergii (C3). Morphological and cultural characteristics of the isolates were than compared. There were no significant differences in the morphological characteristics of conidia and ascospores produced by the three isolates. However, cultural differences were observed among the isolates. Optimum temperatures for mycelial growth of C1, C2, and C3 were 15, 20, and $20^{\circ}$, respectively. C1 produced a few conidia and no ascospores, while C2 and C3 produced abundant ascospores and conidia. While optimum temperatures for mycelial growth ranged from 15 to $20^{\circ}$, mycelial growth was also relatively good at lower temperatures of 5-$10^{\circ}$. Conidiomata and conidia were produced on MSA (malt extract soya peptone agar) after 25-30 days of incubation in the dark at $15^{\circ}$. Apothecia were produced by altering culture condition from 15 to $20^{\circ}$, and incubating for 35-60 more days. Optimum temperature for ascospore and conidium germination was $20^{\circ}$. RAPD analysis revealed that there was high similarity of 0.78 between C2 and C3, and low similarity of 0.31 between C2 or C3 and C1.

• Mycobiology
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• v.42 no.4
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• pp.331-338
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• 2014

To examine the effects of water stress and Cenangium ferruginosum (CF) on the fungal endophytic community of needles of Pinus koraiensis (PK), fungal endophytes isolated from the needles of 5-year-old PK seedlings were compared before and after exposure to water stress conditions and artificial inoculation with CF ascospores. Artificial CF inoculation was successfully confirmed using PCR with CF-specific primers (CfF and CfR). For comparison of the degree of water deficit in water-stressed and control groups of PK seedlings infected with CF, the water saturation deficit and water potential were measured. Lower water potential estimates were found in the water-stressed seedlings than in the control group. The fungal endophytes isolated from the second-year needles of non-water-stressed seedlings before and after CF inoculation revealed that primary saprobes were approximately 30% and 71.7%, respectively, and the remaining endophytes were rot fungi or pathogens. Sixty days after CF inoculation, diverse fungal endophytes in the first-year needles were isolated from the water-stressed seedlings. However, some fungal endophytes isolated from the non-water-stressed seedlings were also identified. Fungal endophytes in the second-year needles of the water-stressed and non-water-stressed seedlings were approximately 8% and 71.7% of saprobes, respectively, and the remaining endophytes were rot fungi or pathogens. On the basis of the results, we conclude that water deficit and CF can have an effect on fungal endophytic communities in the needles of PK seedlings.

• Landscaping Tree
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• s.110
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• pp.18-19
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• 2009

피목가지마름병은 Cenangium ferruginosum에 의하여 발생되는 병해로 전 세계적으로 적송, 곰솔, 잣나무 등의 소나무류에 흔히 발생하며, 특수한 경우를 제외하면 일반적으로 피해는 경미하게 나타난다. 하지만 최근 남부지역 소나무류 집단고사는 강수량 부족에 의한 스트레스가 유인되어 수세가 약화된 상태에서 피목가지마름병의 피해가 가속화된 것으로 추정되며, 이 병은 기상요인과 관련이 높기 때문에 가지치기, 임목밀도조절 등 재배환경을 조절하고 관리가 잘 되도록 신경을 써야 피해를 줄일 수 있다.