• 제목/요약/키워드: Cementum

검색결과 263건 처리시간 0.023초

흡수성 차폐막의 치주조직 재생에 혈소판유래 성장인자가 미치는 영향 (The Effect of PDGF-Loaded Biodegradable Membrane on Early Healing Stage in Guided Tissue Regeneration)

  • 류인철;배규현;설양조;구영;이승진;한수부;최상묵;정종평
    • Journal of Periodontal and Implant Science
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    • 제29권3호
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    • pp.507-519
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    • 1999
  • The ultimate objective of periodontal treatment is to stop disease progression and to regenerate destroyed periodontal tissues and thereby regain normal function. Growth factors are naturally found polypetides which stimulate many cellular activities pertaining to wound healing by acting as signal molecule in controlling cell movement, proliferation, and matrix production. Platelet derived growth factor (PDGF) is 28,000-35,000 Da molecular weight dimeric protein with 2 long positively charged polypeptide chains connected by sulfide bonds. The purpose of this study is to evaluate histologically the initial guided tissue regeneration in a periodontal defect f a beagle dog treated with a biodegradable membrane formed with polylactic acid (poly-L-lactic acid) and polyglycolic acid loaded with 200ng/$cm^2$ platelet derived growth factor. 2 beagle dogs were used in he experiment. $5mm{\times}6mm$ alveolar bone defect was formed in upper and lower canines and third premolars and a reference notch was placed. PDGF-BB non-containing membrane was used as control. Each defect was randomly assigned to the test roup or the control group. The dogs were sacrificed 3 weeks after membrane placement. Toluidine blue and multiple staining was done for histological analysis. In the 3 week specimen in the control group, no new one formation could be seen. Small amount f bone resorption below the notch could be seen. In the notch, loose connective tissue with infiltration of inflammatory cells could be seen. Also thin discontinuous new cementum could be seen and the membrane still retained its structure. Where PDGF-BB containing membrane was used, new bone formation could be seen in the notch at weeks and also continuous thin cementum could be seen. PDL cells were observed between new bone and new cementum and some were attached to bone and cementum. These results suggest that new bone and cementum formation seen when PDGF-BB loaded membrane was used was due to inhibition of downgrowth of epithelial cells and also due to continuous release of the growth factor. Further study on the resorption characteristics of the membrane nd the release characteristics of the PDGF-BB is necessary. Also, development of a membrane easier to use clinically is necessary.

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Periodontal healing using a collagen matrix with periodontal ligament progenitor cells in a dehiscence defect model in beagle dogs

  • Yoo, Seung-Yoon;Lee, Jung-Seok;Cha, Jae-Kook;Kim, Seul-Ki;Kim, Chang-Sung
    • Journal of Periodontal and Implant Science
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    • 제49권4호
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    • pp.215-227
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    • 2019
  • Purpose: To histologically characterize periodontal healing at 8 weeks in surgically created dehiscence defects in beagle dogs that received a collagen matrix with periodontal ligament (PDL) progenitor cells. Methods: The bilateral maxillary premolars and first molars in 6 animals were used. Standardized experimental dehiscence defects were made on the buccal side of 3 premolars, and primary culturing of PDL progenitor cells was performed on the molars. Collagen matrix was used as a scaffold and a delivery system for PDL progenitor cells. The experimental sites were grafted with collagen matrix (COL), PDL progenitor cells with collagen matrix (COL/CELL), or left without any material (CTL). Histologic and histomorphometric analyses were performed after 8 weeks. Results: The defect height from the cementoenamel junction to the most apical point of cementum removal did not significantly differ across the CTL, COL, and COL/CELL groups, at $4.57{\pm}0.28$, $4.56{\pm}0.41$, and $4.64{\pm}0.27mm$ (mean ${\pm}$ standard deviation), respectively; the corresponding values for epithelial adhesion were $1.41{\pm}0.51$, $0.85{\pm}0.29$, and $0.30{\pm}0.41mm$ (P<0.05), the heights of new bone regeneration were $1.32{\pm}0.44$, $1.65{\pm}0.52$, and $1.93{\pm}0.61mm$ (P<0.05), and the cementum regeneration values were $1.15{\pm}0.42$, $1.81{\pm}0.46$, and $2.57{\pm}0.56mm$ (P<0.05). There was significantly more new bone formation in the COL/CELL group than in the CTL group, and new cementum length was also significantly higher in the COL/CELL group. However, there were no significant differences in the width of new cementum among the groups. Conclusions: PDL progenitor cells carried by a synthetic collagen matrix may enhance periodontal regeneration, including cementum and new bone formation.

소구치의 intrusion시 치근 흡수 및 회복에 대한 주사전자 현미경적 연구 (A SCANNING ELECTRON MICROSCOPIC STUDY OF THE ROOT RESORPTION AND REPAIR IN BICUSPID INTRUSION)

  • 구미숙
    • 대한치과교정학회지
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    • 제18권2호
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    • pp.387-404
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    • 1988
  • The purpose of this study was to investigate the root resorption and repair pattern of human teeth under intrusive orthodontic loadings of varying magnitude and duration. 50 gms, 100 gms, 200 gms of force applied to the 96 experimental teeth with duration of 2, 5, 7, 10 weeks, after removing the arch wire and retaining during 2 and 6 weeks, experimental teeth were extracted. Results were as follows: 1. The initial resorption site was visible in the apex within 2 weeks with 50 gms, 100 gms samples. 2. The sites of resorption increased markedly with duration of the force. 3. The 50 grams samples of the all duration reveal the shallow resorption and the 100 gms samples of the all duration except 2 weeks reveal the shallow and deep resorption sites. 4. After 7 weeks with 50 gms and 100 gms, cellular cementum repair accompanied the continuing resorption. 5. 2 and 6 weeks after removing the force with 200 gms, the same cellular cementum repair accompanied with 50 gms, 100 gms samples.

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근관충전제가 치근단위조직에 미치는 영향에 관한 실험적 연구

  • 최호영
    • 대한치과의사협회지
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    • 제18권9호통권136호
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    • pp.739-745
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    • 1980
  • The histologic responses of periapical tissues to root canal fillings with Grossman sealer were studied 10 dogs. Root canal fillings were performed on the 20 lower and upper teeth. The animals were sacrificed 1,2,3,4 and 5 weeks after the completion of operation. The following results were based on histopathologic studies; 1) After 1 week, the necrosis of dentin and cementum surrounding root apex was found in the root canal fillings. 2) After 2 weeks, the necrosis of cementum and surrounding alveolar bone were revealed. The resorption of dentin was appeared partially. 3) After 2 weeks, newly formed dentin was appeared surrounding necrotic dentin. 4) After 4-5 weeks, the osteoblastic activity was revealed abundantly surrounding the alveolar bone. 5) Fibrosis was prominantly appeared surrounding over-filled area, and fibrous encapsulation was performed.

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악안면부의 섬유골성 병소 명칭에 대한 고찰 (Review of nomenclature revision of fibro-ossous lesions in the maxillofacial region)

  • 이병도
    • Imaging Science in Dentistry
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    • 제37권1호
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    • pp.1-7
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    • 2007
  • Fibro-osseous lesions are composed of connective tissue and varying amount of mineralized substances, which may be bony or cementum-like structures. It is necessary for oral radiologist to differentiate due to the tendency of these fibro-osseous lesions to show similar histopathologic appearances, while the management of each lesion is different. However we often encounter a little difficulty in judgement because there are some overlaps between concept of each lesions. So recently I suggest, we face a need to review basic concept and classification of several fibro-osseous jaw lesions. In this article, several fibre-osseous lesions, such as fibrous dysplasia, cemento-ossifying fibroma and cemento-osseous dysplasia, will be discussed basing on the review of literature. particular emphasis will be made on the nomenclature revision of WHO's classification in 1992.

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Treatment of the cemental tear

  • Park, Ye-Sol;Lee, Jae-Hong;Jeong, Seong-Nyum
    • 구강생물연구
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    • 제42권4호
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    • pp.248-253
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    • 2018
  • Cemental tears are uncommon form of root fracture that can lead to rapid localized periodontal attachment loss. Studies have described periodontal breakdown as being associated with the separation of the cementum from the underlying tooth structure. The aim of this case report is to assess the outcome of treatment of cemental tear with several surgical treatment regimens. Three patients with cemental tear were treated with different surgical method. In all three cases, the cemental tear occurred on maxillary right central incisors. In each case, the root fragment were removed, the localized defect was treated using different surgical methods including guided tissue regeneration and bone graft followed by scaling and root planting. In all three cases, symptoms subsided after the treatment and clinical attachment level was improved up to 2 mm at 3 month after surgery. Both conventional and regenerative periodontal surgery could achieve successful outcomes.

뇌성마비 환자의 지연맹출을 동반한 다발성 과백악질증 : 증례보고 (GENERALIZED HYPERCEMENTOSIS WITH ARRESTED DENTAL ERUPTION IN A CEREBRAL PALSY PATIENT : A CASE REPORT)

  • 김별이라;선예지;송제선;이제호
    • 대한장애인치과학회지
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    • 제13권2호
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    • pp.91-94
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    • 2017
  • 본 증례는 다발성 과백악질증 및 영구치의 맹출 지연을 나타내는 뇌성마비 병력 환자에 대해 보고이다. 현재 환자는 큰 불편감 없이 유구치를 유지하고 있으며 방사선 사진 검사를 통해 매복 영구치의 낭종 발생 여부를 확인하고 잔존 유치를 최대한 보존해야 한다.

성견 1면 치조골 결손부에서 특수제조된 Calcium Sulfate Paste가 치주조직 치유에 미치는 영향 (Periodontal repair in dogs: effect of the modified calcium sulfate paste on the 1-wall intrabony defects)

  • 김종관
    • Journal of Periodontal and Implant Science
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    • 제29권1호
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    • pp.153-171
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    • 1999
  • The purpose of this study was to evaluate the effect of modified calcium sulfate paste on periodontal regeneration. l-wall intrabony defect(mesio-distal width: 4mm, depth: 4mm) was surgically created on the distal side of P2 and mesial side of p4 in four dogs. The control group(GFS) was treated with conventional flap operation alone, and the experimental group(CS) was treated with conventional flap operation with modified calcium sulfate paste application. Both control and experimental groups were sacrificed after 8weeks of healing period, The results of histological and histometric observations were as follows. 1. The length of the junctional epithelium was 0.41${\pm}$0.01mm in the control groups, 0.47${\pm}$0.01mm in the experimental group. 2. The connective tissue attachment was 0.28${\pm}$0.02mm(6.15${\pm}$0.28%) in the control group, 0.18${\pm}$0.01mm(3.41${\pm}$0.14%) in the experimental group. The control group showed more connective tissue attachment. 3. The new cementum formation was 3.80${\pm}$0.06mm(84.80${\pm}$0.33%) in the control group, 4.49${\pm}$0.06mm(87.57${\pm}$0.15%) in the experimental group. Both groups showed a lot of new cementum formation. 4. The new bone formation was 1.43${\pm}$0.03mm(32.37%) in the control group, 2.04${\pm}$O.09mm(40.94%) in the experimental group. 5. The inflamatory cells were observed partially around resorbed calcium sulfate in the connective tissue of the experimental group. 6. Partially resorbed calcium sulfate were found within the connective tissue, around alveolar bone, and in the newly formed alveolar bone, On the basis of these results, newly formed calcium sulfate paste enhanced new bone formation and new cementum formation. The resorption rate of calcium sulfate seems to be controlled by the add-in compounds. Thus research about biocompatibility and adequate resorptionrate is required to develop a improved material.

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성견의 3급 이개부 병변에서 성형성 합성 펩타이드 젤의 치주재생 능력에 관한 실험적 연구 (Experimental study on the periodontal regenerative capacity of moldable synthetic peptide domain gel in degree III furcation defect of beagles)

  • 김정범;박윤정;이상철;김태일;설양조;이용무;구영;류인철;한수부;정종평
    • Journal of Periodontal and Implant Science
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    • 제38권4호
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    • pp.621-628
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    • 2008
  • Purpose: Osteopontin is one of the major non-collagenous protein of hard tissue. Use of peptide domain of biologically active protein has some advantages. The objective of this experimental study is evaluation of periodontal regenerative potency of synthetic peptide gel which containing collagen binding domain of osteopontin in the degree III periodontal defect of beagle dogs. Material and Methods: Experimental degree III furcation defect was made in the mandibular third and fourth premolar of beagles. Regenerative material was applied during flap operation. 8 weeks after regenerative surgery, all animals were sacrificed and histomorphometric measurement was performed to calculate the linear percentage of the new cementum formation and the volume percentage of new bone formation. Result: The linear percent of new cementum formation was 41.6% at control group and 67.1% at test group and there was statistically significant difference. The volume percent of new bone formation was 52.1% at control group and 58.9% at test group. Conclusion: As the results of present experiment, synthetic peptide gel containing collagen binding domain of osteopontin significantly increase new bone and cementum formation in the degree III furcation defect of canine mandible.