• 한국동물학회지
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• 제15권3호
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• pp.101-104
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• 1972

간편하고 경제적이며 재현성도 우수한 cellulose acetate 전기영동장치를 제작하였다. 본 장치를 사용하여 몇가지 동물 조직의 lactate 및 malate dehydrogenase isozyme 을 분리 검출하여 본 결과, 기존 제품으로 실험한 결과와 별로 차이가 없음을 알았다. 흔히 cellulose acetate 전기영동은 일정한 정전류에서 실시하는데, 본 장치에서는 약간의 전류변동이 있는 것이 사실이나 실험결과는 충분히 실용적인 것이었다.

• 대한수의학회지
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• 제24권1호
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• pp.31-35
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• 1984

The hemoglobin phenotype and the gene frequencies of 44 Formosan deer(Cervus nippon) in Kyung-Gi area were examined by using cellulose acetate and starch gel electrophoresis. 1. The method of cellulose acetate electrophoresis was simplier, more clear and preserative than starch gel electrophoresis. 2. The hemoglobin phenotype was appeared 3 types as $Hb^F$ $Hb^{FS}$ and $Hb^S$. The frequencies of appearance were $Hb^F$ 47.7%, $Hb^{FS}$ 47.7% and $Hb^S$ 4.5%, respectively. 3. The genetic factors of hemoglobin were observed as $Hb^F$ and $Hb^S$ and the rates of gene frequencies were 71.6% and 28.4%, respectively.

• 생약학회지
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• 제33권3호통권130호
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• pp.257-261
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• 2002

Each ferritin molecule consists of light subunit 19,000 dalton and heavy subunit 22,000 dalton. Twenty-four protein subunit about $440,000{\sim}500,000$ dalton apoferritin which contained $20{\sim}30%$ Fe as ferric hydroxyphosphate polymer form. Horse spleen-derived ferritin consists of 90% light subunit. These genetic characteristics of ferritin preparations were able to determine by cellulose acetate electrophoresis, but these ferritin preparations contained other components to be disturbed during refining, extraction and making finish products and have difficulties in deciding to be just. So, this study was performed to establish the scientific method for determine the quality of ferritin preparations with immunodiffusion methods which has high specificity between heterogeneous proteins.

• 한국동물학회지
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• 제17권4호
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• pp.159-162
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• 1974

韓國産 개구리 目의 8種에 대하여 血色素와 血淸 단백질의 cellulose acetate 電基泳動圖를 조사하였다. 혈색소의 전기영동도는 종에 따라 그 구성성분의 수와 이동도에 차이가 있었다. 두꺼비와 금개구리에서는 단일 밴드로 나타났고, 여타의 종에서는 2밴드로 분리되었다. 혈청단백질의 전기영동도는 종에 따른 특징을 잘 나타내고 있다. 이들 동물의 혈청단백질에서는 다형현상이 나타나지 않았다. 모든 실험재료에서 albumin은 뚜렷이 나타나지만 prealbumin은 나타나지 않았다. 두꺼비에서는 albumin과 postalbumin 사이가 분명하게 분리되지 않았다. 따라서 혈청단백질의 전기영동도에서 두꺼비와 다른 종들을 쉽게 구별할 수가 있었다.

• 대한수의학회지
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• 제31권1호
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• pp.99-108
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• 1991

To observe the changes of serum proteins according to the process of Babesia gibsoni(B gibsoni) infection, the babesia protozoa($10^8/kg$) were inoculated into the cephalic vein of healthy dogs. The serum proteins of experimentally infected dogs were separated by using cellulose acetate electrophoresis. The results obtained were as follows; 1. Cellulose acetate electrophoresis was fractionated to total 6 of bands such as, albumin, ${\alpha}_1$, ${\alpha}_2$, ${\beta}_1$, ${\beta}_2$ and $\gamma$-globulin. 2. The concentration of total protein was shown a decreasing tendency after B gibsoni infection. Albumin and A/G ratio were lowered through all periods of the infection, but they were not significant changes. 3. The level of ${\alpha}_1$-globulin was significantly(p<0.05) incresed in early stage of the infection. 4. The levels of ${\alpha}_2$ and total $\alpha$-globulin were shown highly significant decreases (p<0.01) through all periods of the infection. 5. The levels of ${\beta}_1$ and total $\beta$-globulin had highly significant changes (p<0.01) that was increased in early stage of infection and decreased later. 6. The level of $\gamma$-globulin was seen to be constantly increased through all periods of infection. It was a highly significant change (p<0.01). 7. Plasma protein: fibrinogen (PP:F) ratio was shown a temporally significant increase (p<0.05) following the decrease in early infection.

• Asian-Australasian Journal of Animal Sciences
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• 제19권4호
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• pp.601-604
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• 2006

The objective of this study was to investigate technical methods for extraction of mucopolysachharide-protein containing chondroitin sulfate from keel cartilage of chickens. The chemical composition of chicken keel cartilage was determined. For the preparation of mucopolysaccharide-protein from lyophilized chicken keel cartilage, hot water extraction and alcalase hydrolysis methods were examined. Results showed that the optimum condition of hot water extraction was incubation for 120 min with a yield of 40.09% and chondroitin sulfate content of 28.46%. For alcalase hydrolysis, the most effective condition was 2% alcalase in 10 volumes of distilled water for 120 min. The yield of hydrolysate was 75.87%, and chondroitin sulfate content was 26.61%. For further separation of chondroitin sulfate from the alcalase hydrolysate, which has a higher yield than that of hot water, 60% ethanol precipitation was performed. The yield of the ethanol precipitate was 21.41% and its chondroitin sulfate content was 46.31%. The hot water extract, alcalase hydrolysate and ethanol precipitate showed similar electrophoretic migration with standard chondroitin sulfate (chondroitin sulfate A), using cellulose acetate membrane electrophoresis. These results indicated that a significant amount of mucopolysaccharide-protein containing chondroitin sulfate could be acquired form chicken keel cartilage. Therefore, keel cartilage in chicken may provide an inexpensive source of chondroitin sulfate for commercial purposes.

• 한국동물학회지
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• 제16권1호
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• pp.67-77
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• 1973

한국산 초파리(Drosophila melanogaster)의 starch gel 영동상을 조사하기 위하여 춘천, 진주, 신촌(서울), 대전의 초파리 4계통을 starch gel media를 사용하여 벤드의 분포, 염색강도 및 이동도를 비교 검토한 결과는 다음과 같다. 1) 춘천, 진주, 신촌(서울)의 3계통은 벤드의 수와 분포, 염색강도, 이동도가 다 같이 심한 변이를 나타내고 있는데 반하여 대전계통은 변이가 거의 없다. 2) 이러한 전기영동상의 차이는 대전 계통의 초파리 집단이 ADH구성에 관하여 동질적(homogeneous)이고 다른 3계통의 집단은 이질적(heterogeneous)이라는 것을 암시하고 있다. 3) 전기영동상이 4계통의 초파리에서 다 같이 암$\\cdot$수에 따라 차이를 보이고 있는데 이것은 설명하기 매우 어려운 문제로서 장차 분석 검토할 필요가 있다고 본다. 4) Starch gel media가 cellulose acetate media보다 해상력이 월등하다는 것이 재확인 되고 있다. 5) Agar gel이나 acrylamide gel 같은 다른 영동 media를 써서 ADH의 전기영동 실험을 하여 starch gel media를 사용한 본 실험의 결과와 비교하는 것과 ADH에 관한 유전적 분석이 시급히 요구되는 앞으로의 과제이다.

• 약학회지
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• 제16권1호
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• pp.34-46
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• 1972

Lactate dehydrogenase isozymes in heart, kidney, liver and skeletal muscle of 15 species of vertebrate animals belonging to 5 classes were separated by cellulose acetate electrophoresis and the levels of them were measured and compared with each other. Lactate dehydrogenase isozyme patterns were different from each other among animal species and among tissues. The activity of LDH$_{5}$ was superior in anaerobic tissues such as liver and skeletal muscle, and the activity of LDH$_{1}$ was superior in aerobic tissues such as heart and kidney. The level of LDH of vertebrate animals of the 5 classes has found approximatry increasing in the following order: Pisces>Amphibia>Reptelia

• Restorative Dentistry and Endodontics
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• 제2권1호
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• pp.20-26
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• 1976

Cellulose acetate electrophoresis was used for differential diagnosis of radicular cysts and granulomas. Sixteen periapical lesions were excised from sixteen teeth with radiographic evidence of periapical pathosis, and two normal periodontal tissues were surgically removed from two wisdom teeth. The tissue samples were separated into two parts. Half was prepared for cellulose acetate electrophoresis, and the electrophoretic patterns were scanned with Helena densitometer. The other half was examined histologically, and the histologic diagnoses were then compared with the electrophoretic patterns and with the x-ray findings. The results were the following: 1. The histopathologic results showed the presence of 13 granulomas (81.8%) and 3 cysts (18.3%). 2. An albumin pattern, alpha-globulin pattern, beta-globulin pattern, fibrinogen pattern and gamma-globulin pattern were found in all eighteen cases. 3. All eighteen cases were divided into normal periodontal tissue group, dental granuloma group, and radicular cyst group by the histopathologic diagnosis. The electrophoretic pattern of dental granuloma group and that of normal periodontal tissue group resembled each other. The two groups showed the highest percentage in beta-globulin fraction, and the beta-globulin of dental granuloma group(47.17%) was higher than that of normal periodontal tissue group (40.54%) by 6.63%. On the contrary, beta-globulin fraction of radicular cyst group (28.00%) was much lower than those of the granuloma group (47.17%) and normal periodontal tissue group (40.54%), and alpha-globulin of radicular cyst group (34.16%) was much higher than those of the granuloma group (20.04%) and normal group (19.58%). 4. Five cases (31.3%) of the sixteen periodontal lesions showed different results between histopathologic diagnoses and radiographic findings.

• 대한치과의사협회지
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• 제15권12호
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• pp.1031-1035
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• 1977

Acidic glycosaminoglycans were isolated from the bovine gingiva and analyzed by chemical methods and by two dimensional electrophoresis on cellulose acetate strip. The average total amount of acidic glycosaminoglycans-expressed as glucuronic acid-was 0.36% of dry gingival tissue. By using colorimetric analysis with two dimenional electrophoresis, the distribution of dermatan sulfate was calculated to be 33% of whole acidic glycosaminoglycans, chondroitin sulfate A to be 26% ad hyaluronic acid to be 38%, respectively.