Browse > Article

Identification of Ferritin Using Immunodiffusion Methods  

Ha, Kwang-Won (Korea Food and Drug Administration)
Cho, Jung-Hee (Korea Food and Drug Administration)
Kim, Do-Hoon (Korea Food and Drug Administration)
Kim, Young-Lim (Korea Food and Drug Administration)
Kim, Hong-Jin (College of Pharmacy, Chung-Ang Univ.)
Shim, Young-Hun (Korea Food and Drug Administration)
Publication Information
Korean Journal of Pharmacognosy / v.33, no.3, 2002 , pp. 257-261 More about this Journal
Abstract
Each ferritin molecule consists of light subunit 19,000 dalton and heavy subunit 22,000 dalton. Twenty-four protein subunit about $440,000{\sim}500,000$ dalton apoferritin which contained $20{\sim}30%$ Fe as ferric hydroxyphosphate polymer form. Horse spleen-derived ferritin consists of 90% light subunit. These genetic characteristics of ferritin preparations were able to determine by cellulose acetate electrophoresis, but these ferritin preparations contained other components to be disturbed during refining, extraction and making finish products and have difficulties in deciding to be just. So, this study was performed to establish the scientific method for determine the quality of ferritin preparations with immunodiffusion methods which has high specificity between heterogeneous proteins.
Keywords
Ferritin; Cellulose acetate electrophoresis; Immunodiffusion;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kitasato University (1997) Sequencing of cDNA Clones that encode bovine ferrtin H and L chains, 118(3): 667-73   DOI   ScienceOn
2 Edelman, G. M. (1973) The Structure and Function of Antibodies, Sci. Am., 223: 34-42   DOI   ScienceOn
3 European Pharmacopoea (1997), Biological assays-Immuno-chemical methods, pp. 103-104
4 Dickerson, R. E. (1972) Structuren and History of an Ancient Protein, Sci. Am., 226: 58-72   DOI   ScienceOn
5 Eli B., Sidney L. (1991) Immunology A Short Course, Wiley-Liss