• YAKHAK HOEJI
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• v.54 no.1
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• pp.8-12
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• 2010

Inositol 1,4,5-trisphosphate ($IP_3$) receptor ($IP_3R$)-mediated signaling pathway is involved in many cellular processes including fertilization, apoptosis and neuronal function. Although cardiac myocytes express the $IP_3R$, its pathophysiological role has not been clearly understood because of limited selectivity of currently available pharmacological blockers. In the present study we constructed shRNA-expressing adenovirus to knock-down the type 2 $IP_3R$ ($IP_3R2$), a major subtype in cardiac ventricular myocytes, and demonstrated that the virus successfully eliminated the expression and localization of the $IP_3R2$. These results may provide a reliable tool for probing pathophysiological roles of the $IP_3R2$ in isolated intact cardiac myocytes.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.12C
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• pp.1245-1250
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• 2002

For recent years, the environment of use on internet has been changed from the wired internet access to the wireless internet access increasingly, and the use of real time application data has been increased. Therefore the research and evolution of wireless network for providing of user's mobility and QoS at the same time must be essential. IETF already proposed Mobile IPv6 for providing mobility and RSVP for QoS through resource reservation. These future are expected to be used not only wired network but also wireless network. But there are not yet proposed the integration and efficient interworking of two mechanism. For the solution of problem on efficient interworking issue, for instance signaling overhead and reservation delay increasing due to often handoff, this paper first proposed to use of IETF Cellular IP based on Mobile IPv6 for wireless network model with fast mobility and a improved RSVP mechanism using the flow label field in IPv6 header.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.11A
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• pp.1101-1112
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• 2006

With the recent growth in demand for high-data rate multimedia services in the wireless environments, the Mobile Broadband Wireless Access (MBWA) technologies, such as WiBro (Wireless Broadband internet) system, are gradually coming into the spotlight. Unlike the conventional mobile communication networks based on cellular system, the WiBro system basically consists of IP based backbone networks that will be ultimately deployed by Ipv6 (IP version six) based backbone networks according to the All-IP trend for the network evolution. In such wireless mobile environments, it is needed to support the mobility management protocol on network layer as well as physical layer and Medium Access Control (MAC) layer in WiBro system. Accordingly, in this paper, we propose a fast handover scheme for improving the handover performance in IPv6 based WiBro system and show that the proposed scheme achieves loss-free and low handover latency during inter-subnet movement of the mobile stations through the simulation.

• Journal of Microbiology and Biotechnology
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• v.18 no.8
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• pp.1364-1367
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• 2008

Oxidative stress damages all cellular constituents, and therefore, cell has to possess various defense mechanisms to cope. Saccharomyces cerevisiae, widely used as a model organism for studying cellular responses to oxidative stress, contains three glutathione peroxidase (Gpx) proteins. Among them, Gpx3 plays a major defense role against oxidative stress in S. cerevisiae. In this study, in order to identity the new interaction proteins of Gpx3, we carried out two-dimensional gel electrophoresis after immunoprecipitation (IP-2DE), and MALDI-TOF mass spectrometry. The results showed that several proteins including protein disulfide isomerase, glutaredoxin 2, and SSY protein 3 specifically interact with Gpx3. These findings led us to suggest the possibility that Gpx3, known as a redox sensor and ROS scavenger, has another functional role by interacting with several proteins with various cellular functions.

• 한국정보통신설비학회:학술대회논문집
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• 2005.08a
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• pp.318-322
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• 2005

An IP address plays both endpoint identifier and location identifier in wire based network. However, one IP address can not provide both endpoint identifier and location identifier for mobile Internet environment such as cellular network and portable Internet. To resolve this problem, mobile IPv4 and mobile IPv6 technologies are developed. These technologies are based on the concept of separation the endpoint identifier from location identifier. This paper describes some results of the recent studies such as mobile IPv4, mobile IPv6, host identify protocol and so on. Also, we propose the criteria to decide which technologies are suitable for deployment in the practical network environment.

• Information and Communications Magazine
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• v.24 no.7
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• pp.79-88
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• 2007

본고에서는 현재 일반적으로 가장 널리 사용되고 있는 네트워크 시뮬레이터인 NS-2(Network Simulator-2)에 대하여 알아본다. NS-2는 네트워킹의 성능분석을 목적으로 개발된 이벤트 기반(event-driven)의 시뮬레이터이다. 이 시뮬레이터의 적용 범위는 유선 네트워크의 경우 TCP/IP 프로토콜 패밀리와 각종 라우팅 프로토콜에 대한 시뮬레이션이 가능하며, 무선 네트워크에 경우에는 Ad Hoc 네트워크, WLAN, Mobile-IP와 Cellular network 등의 시뮬레이션이 가능하다. GUI가 부족하고 사용이 어렵다는 단점에도 불구하고, 공개된 소스와 두터운 이용자층에 힘입어 널리 사용되는 NS-2의 개요 및 시뮬레이션 방법에 대해 알아보기로 한다.

• Journal of Animal Science and Technology
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• v.51 no.4
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• pp.289-294
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• 2009

Inositol 1,4,5-triphosphate receptor type 1 (IP3R1) is a $Ca^{2+}$ release channel that responds to the second messenger IP3 and that modulates diverse cellular functions such as contraction/excitation, secretion, gene expression and cellular growth. We discovered single nucleotide polymorphisms (SNPs) within IP3R1 gene and analyzed associations between gene polymorphisms and carcass traits in Korean cattle (Hanwoo) in order to develop novel DNA markers at genomic level. Three SNPs were detected at the position of g.1428617A>G, g.1418843C>T and g.1414377C>T with 24 unrelated Hanwoo samples by direct sequencing of the PCR products. We found that genotype of g.1414377C>T SNP was associated with live weight (P<0.05) and carcass weight (P<0.01) using the general linear model of SAS package. These results suggest that polymorphism of IP3R1 gene was associated with weight-related traits in Hanwoo.

• The Korean Journal of Physiology and Pharmacology
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• v.16 no.1
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• pp.31-36
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• 2012

The receptor activator of NF-${\kappa}B$ ligand (RANKL) signal is an activator of tumor necrosis factor receptor-associated factor 6 (TRAF6), which leads to the activation of NF-${\kappa}B$ and other signal transduction pathways essential for osteoclastogenesis, such as $Ca^{2+}$ signaling. However, the intracellular levels of inositol 1,4,5-trisphosphate ($IP_3$) and $IP_3$-mediated cellular function of RANKL during osteoclastogenesis are not known. In the present study, we determined the levels of $IP_3$ and evaluated $IP_3$-mediated osteoclast differentiation and osteoclast activity by RANKL treatment of mouse leukemic macrophage cells (RAW 264.7) and mouse bone marrow-derived monocyte/macrophage precursor cells (BMMs). During osteoclastogenesis, the expression levels of $Ca^{2+}$ signaling proteins such as $IP_3$ receptors ($IP_3Rs$), plasma membrane $Ca^{2+}$ ATPase, and sarco/endoplasmic reticulum $Ca^{2+}$ ATPase type2 did not change by RANKL treatment for up to 6 days in both cell types. At 24 h after RANKL treatment, a higher steady-state level of $IP_3$ was observed in RAW264.7 cells transfected with green fluorescent protein (GFP)-tagged pleckstrin homology (PH) domains of phospholipase C (PLC) ${\delta}$, a probe specifically detecting intracellular $IP_3$ levels. In BMMs, the inhibition of PLC with U73122 [a specific inhibitor of phospholipase C (PLC)[ and of $IP_3Rs$ with 2-aminoethoxydiphenyl borate (2APB; a non-specific inhibitor of $IP_3Rs$) inhibited the generation of RANKL-induced multinucleated cells and decreased the bone-resorption rate in dentin slice, respectively. These results suggest that intracellular $IP_3$ levels and the $IP_3$-mediated signaling pathway play an important role in RANKL-induced osteoclastogenesis.

• Molecules and Cells
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• v.40 no.6
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• pp.418-425
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• 2017

Interferon-${\gamma}$-inducible protein 10 (IP-10), also known as chemokine C-X-C motif ligand (CXCL) 10, is closely associated with antiviral immunity and the progression of chronic hepatitis B (CHB). However, the value of baseline serological and histological IP-10 expression levels in predicting the efficacy of the antiviral response to nucleoside/nucleotide analogues (NAs) is still unknown. In our research, intrahepatic and peripheral IP-10 expression levels were systemically examined before and after treatment with entecavir (ETV). Baseline serological and histological IP-10 expression levels were significantly increased in patients with CHB, particularly in patients with higher degrees of liver inflammation and liver fibrosis. Moreover, higher baseline intrahepatic IP-10 levels indicated better prognoses in patients with CHB after entecavir therapy. The baseline IP-10 level was also positively associated with several clinical parameters, including baseline levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatitis B virus (HBV) DNA, and hepatitis B surface antigen (HBsAg), and with the decrease in HBsAg levels after treatment. In addition, monocyte-derived IP-10 was expressed at higher levels in patients with CHB than in patients with liver cirrhosis (LC) and healthy controls (HC). According to the results of our in vitro experiments, IP-10 directly promoted hepatocyte apoptosis. Based on these findings, baseline serological and histological IP-10 levels might predict CHB severity and the decrease in HBsAg levels after entecavir therapy.

• The Journal of the Korea Contents Association
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• v.8 no.2
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• pp.67-73
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• 2008

Recently, due to the rapid advances of mobile communication, multimedia service can be provided by mobile devices. Cellular network tends to uses SIP as a call setup protocol in order to provide various multimedia service to consumers. Cellular network holds the characteristic of higher BER(Bit Error Rate), narrower bandwidth than the wired network. The value of SIP RTI(Retransmission Time interval) based on the wired network decreases a network efficiency and increases a call setup delay over cellular network. By using NS-2 simulator, we show new SIP RTI value adequate over cellular network. We design and implement home network by using the modified SIP that is suitable for cellular network.