• Title/Summary/Keyword: Cellular Decomposition

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Screening and Identification of the Yeasts for Orange Wine and Their Citric Acid Decomposition (밀감양조주 생산용 효모의 선별, 동정 및 Citric Acid 분해)

  • Ko, Young-Hwan;Kim, Jae-Ha;Koh, Jeong-Sam;Kim, Chang-Jin
    • Korean Journal of Food Science and Technology
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    • v.29 no.3
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    • pp.588-594
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    • 1997
  • Yeast strains useful for the production of wine using mandarine orange, Citrus unshiu, as a main substrate were screened, and their primary ability to decompose citric acid that affects directly wine quality was investigated. Total eleven strains were selected for brewing orange wine. Five wild strains were from soil-based collections and identified: four of them were Saccharomyces cerevisiae and one of them was S. ellipsoideus. The rest of six strains were from among eighteen laboratory strains: three of them were S. cerevisiae, and the other three were S. coreanus, S. uvarum, and S. sake. Two strains of S. cerevisiae out of these selections were chosen and their decomposition of citric acid was investigated. Citric acid was not utilized as sole carbon source for cellular growth. However, when both citric acid and glucose were added together as carbon sources, decrease of citric acid concentration was observed after incubation. Shaking incubation was more effective for the reduction of citric acid than standing incubation. Utilization of citric acid did not contribute to the increase of ethanol concentration during fermentation. On the other hand, it appeared that citric acid caused partial inhibition of cellular growth of the yeasts.

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Path Planning for an Intelligent Robot Using Flow Networks (플로우 네트워크를 이용한 지능형 로봇의 경로계획)

  • Kim, Gook-Hwan;Kim, Hyung;Kim, Byoung-Soo;Lee, Soon-Geul
    • The Journal of Korea Robotics Society
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    • v.6 no.3
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    • pp.255-262
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    • 2011
  • Many intelligent robots have to be given environmental information to perform tasks. In this paper an intelligent robot, that is, a cleaning robot used a sensor fusing method of two sensors: LRF and StarGazer, and then was able to obtain the information. Throughout wall following using laser displacement sensor, LRF, the working area is built during the robot turn one cycle around the area. After the process of wall following, a path planning which is able to execute the work effectively is established using flow network algorithm. This paper describes an algorithm for minimal turning complete coverage path planning for intelligent robots. This algorithm divides the whole working area by cellular decomposition, and then provides the path planning among the cells employing flow networks. It also provides specific path planning inside each cell guaranteeing the minimal turning of the robots. The proposed algorithm is applied to two different working areas, and verified that it is an optimal path planning method.

Optimal Underwater Coverage of a Cellular Region by Autonomous Underwater Vehicle Using Line Sweep Motion

  • Choi, Myoung-Hwan
    • Journal of Electrical Engineering and Technology
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    • v.7 no.6
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    • pp.1023-1033
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    • 2012
  • An underwater planar covering problem is studied where the coverage region consists of polygonal cells, and line sweep motion is used for coverage. In many subsea applications, sidescan sonar has become a common tool, and the sidescan sonar data is meaningful only when the sonar is moving in a straight line. This work studies the optimal line sweep coverage where the sweep paths of the cells consist of straight lines and no turn is allowed inside the cell. An optimal line sweep coverage solution is presented when the line sweep path is parallel to an edge of the cell boundary. The total time to complete the coverage task is minimized. A unique contribution of this work is that the optimal sequence of cell visits is computed in addition to the optimal line sweep paths and the optimal cell decomposition.

An Adaptive Adjacent Cell Interference Mitigation Method for Eigen-Beamforming Transmission in Downlink Cellular Systems (하향 링크 셀룰러 시스템의 Eigen-Beamforming 전송을 위한 적응적 인접 셀 간섭 완화 방법)

  • Chang, Jae-Won;Kim, Se-Jin;Kim, Jae-Won;Sung, Won-Jin
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.20 no.3
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    • pp.248-256
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    • 2009
  • EB(Eigen-Beamforming) has widely been applied to MIMO(Multiple-Input Multiple-Output) systems to form beams which maximize the effective signal-to-interference plus noise ratio(SINR) of the receiver using the singular value decomposition(SVD) of the MIMO channel. However, the signal detection performance for the mobile station near the cell boundary is severely degraded and the transmission efficiency decreases due to the influence of the interference signal from the adjacent cells. In this paper, we propose an adaptive interference mitigation method for the EB transmission, and evaluate the reception performance. In particular, a reception strategy which adaptively utilizes optimal combining(OC) and minimum mean-squared error for Intercell spatial demultiplexing(MMSE-lSD) is proposed, and the reception performance is investigated in terms of the effective SINR and system capacity. For the average system capacity, the proposed adaptive reception demonstrates the performance enhancement compared to the conventional EB reception using the receiver beamforming vector, and up to 2 bps/Hz performance gain is achieved for mobile station located at the cell edge.

A Space Division Multiple Access Technique for Downlink MIMO Systems (하향링크 MIMO 시스템을 위한 공간분할 다중접속 기술)

  • Rim, Min-Joong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.9A
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    • pp.1022-1030
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    • 2004
  • The next generation cellular radio systems require high data rate transmission and large system capacity In order to meet these requirements, multiple antennas can be used at the base and mobile stations, forming MIMO(multiple-input, multiple-output) channels This paper considers a downlink MIMO system assuming a large number of base station antennas, a small number of mobile station antennas, and rich-scattering, quasi-stationary, and flat-fading channel environments When the channel state information is given at the base station in a single user system, a MIMO technique with SVD(singular value decomposition) and water-filling can achieve the maximal downlink channel capacity. In multi-user environments, however, SDMA(space division multiple acces) technique can be used to further increase the total channel capacity supported by the base station This paper proposes a MIMO SDMA technique which can transmit parallel data streams to each of multiple users. The proposed method. can achieve higher total channel capacity than SVD-based MIMO techniques or conventional SDMA using smart antennas.

Glucose Deprivation and Immunostimulation Induced Death in Rat Primary Astrocytes is Mediated by Their Synergistic Effect on the Decrease in Cellular ATP Level

  • Choi, Ji-Woong;Yoo, Byoung-Kwon;Yoon, Seo-Young;Jeon, Mi-Jin;Ko, Kwang-Ho
    • Biomolecules & Therapeutics
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    • v.12 no.1
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    • pp.25-33
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    • 2004
  • In this study we investigated whether ATP loss was involved in the potentiated death of immunostimulated rat primary astrocytes in glucose-deprived condition. Rat primary astrocytes immunostimulated with LPS plus IFN-${\gamma}$ for 48 h underwent death upon glucose deprivation, which dependent on the production of peroxynitrite. Intracellular ATP level synergistically decreased by glucose deprivation in immunostimulated astrocytes but not in control cells, and the loss of ATP occurred well ahead of the LDH release. The synergistic cell death and ATP loss by immunostimulation and glucose deprivation were inhibited by iNOS inhibitor (L-NAME and L-NNA) or peroxynitrite decomposition catalyst (also a superoxide anion scavenger), Mn(III)tetrakis(N-methyl-4'-pyridyl)porphyrin (MnTMPyP). Exogenous addition of peroxynitrite generator, SIN-l timedependently induced ATP loss and cell death in the glucose-deprived astrocytes. Depletion of intracellular glutathione (GSH) and dis겨ption of mitochondrial transmembrane potential (MTP) were also observed under same conditions. Supply cellular ATP by the addition of exogenous adenosine or ATP during glucose deprivation inhibited ATP depletion, GSH depletion, MTP disruption and cell death in SIN-l treated or immunostimulated astrocytes. This study showed that perturbation in the regulation of intracellular ATP level in immunostimulated astrocytes might make them more vulnerable to energy challenging stimuli.

The Impact on Water Quality from Blue-Green Algae Microcystis Natural Phytoplankton by Algal Assay (생물검정에 의한 남조류 Microcystis가 수질에 미치는 영향)

  • Shin, Jae-Ki;Cho, Kyung-Ja
    • Journal of Environmental Science International
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    • v.9 no.3
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    • pp.267-273
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    • 2000
  • In order to understand the impact for decomposition of blue-green algae Microcystis on water quality, the algae were cultivated with collection of natural population during approximately one month, when water-bloom of Microcystis dominated at August 31, 1999 in the lower part of the Okchon Stream. The enrichment of inorganic NㆍP nutrients didn't in algal assay and the effect of Microcystis on water duality was assessed from the variation of nutrients by algal senescence. Microcystis population seemed to play a temporary role of sink for nutrients in the water body. Initial algal density of Microcystis was 2.3×10/sup 6/ cells/㎖. When Microcystis population died out under light condition, algal NㆍP nutrients between 9∼12 days affected to increase of biomass after reuse by other algal growth as soon as release to the ambient water. However, cellular nutrients under dark condition were almost moved into the water during algal cultivation. NH₄, NO₃ and SRP concentration were highly increased with 160, 17 and 79 folds, respectively relative to the early. As a result, the senescence of Microcystis population seemed to be an important biological factor in which cause more eutrophy and increase of explosive algal development by a lot of nutrients transfer to water body. There are significantly observed an effort of reduce for production of inner organic matters such a phytoplankton as well as load pollutants from watershed in side of the water quality management of reservoir.

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Rapd Analysis of Trichoderma Isolates for Superior Selection for Biopesticide Preparation

  • Parvin, Shahnaj;Islam, Abu Taher Mohammad Shafiqul;Siddiqua, Mahbuba Khatoon;Uddin, Mohammad Nazim;Meah, Mohammad Bahadur
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.1
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    • pp.35-43
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    • 2011
  • Thirty five isolates of Trichoderma species collected from seven different locations of Bangladesh were studied for morphological characters and molecular variation. Mycelial diameters of the isolates varied from 8.28 cm to 9.00 cm. Based on colony colour, isolates were grouped into five such as dark green, green, light green, yellowish green and whitish green. Maximum isolates were green and light green. On the basis of growth habit and colony consistency, the isolates were categorized into three groups, in which most species had fast growth and were compact in appearance. PCR-based Random Amplified Polymorphic DNA (RAPD) technique employing 3 decamer primers produced 36 scorable bands of which all (100%) were polymorphic. The co-efficient of gene differentiation (Gst) was 1.0000 reflecting the existence of high level of genetic diversity among the isolates. The Unweighted Pair Group Method of Arithmetic Means (UPGMA) dendrogram constructed from Nei's (1972) genetic distance produced 2 main clusters (13 isolates in cluster 1 and 22 isolates in cluster 2). The result indicating their genetic diversity has opened new possibility of using the most efficient and more isolates of Trichoderma in the preparation of biopesticide and decomposition of municipality waste.

Comparison of Hydroxyl Radical, Peroxyl Radical, and Peroxynitrite Scavenging Capacity of Extracts and Active Components from Selected Medicinal Plants

  • Kwon, Do-Young;Kim, Sun-Ju;Lee, Ju-Won;Kim, Young-Chul
    • Toxicological Research
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    • v.26 no.4
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    • pp.321-327
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    • 2010
  • The ability of 80% ethanol extracts from five medicinal plants, Aralia continentalis, Paeonia suffruticosa, Magnolia denudata, Anemarrhena asphodeloides, and Schizonepeta tenuifolia, to neutralize hydroxyl radical, peroxyl radical and peroxynitrite was examined using the total oxyradical scavenging capacity (TOSC) assay. Peroxyl radical was generated from thermal homolysis of 2,2'-azobis(2-methylpropionamidine) dihydrochloride (ABAP); hydroxyl radical by an iron-ascorbate Fenton reaction; peroxynitrite by spontaneous decomposition of 3-morpholinosydnonimine N-ethylcarbamide (SIN-1). The oxidants generated react with $\alpha$-keto-$\gamma$-methiolbutyric acid (KMBA) to yield ethylene, and the TOSC of the substances tested is quantified from their ability to inhibit ethylene formation. Extracts from P. suffruticosa, M. denudata, and S. tenuifolia were determined to be potent peroxyl radical scavenging agents with a specific TOSC (sTOSC) being at least six-fold greater than that of glutathione (GSH). These three plants also showed sTOSCs toward peroxynitrite markedly greater than sTOSC of GSH, however, only P. suffruticosa revealed a significant hydroxyl radical scavenging capacity. Seven major active constituents isolated from P. suffruticosa, quercetin, (+)-catechin, methyl gallate, gallic acid, benzoic acid, benzoyl paeoniflorin and paeoniflorin, were determined for their antioxidant potential toward peroxynitrite, peroxyl and hydroxyl radicals. Quercetin, (+)-catechin, methyl gallate, and gallic acid exhibited sTOSCs 40~85 times greater than sTOSC of GSH. These four components also showed a peroxynitrite scavenging capacity higher than at least 10-fold of GSH. For antioxidant activity against hydroxyl radical, methyl gallate was greatest followed by gallic acid and quercetin. Further studies need to be conducted to substantiate the significance of scavenging a specific oxidant in the prevention of cellular injury and disease states caused by the reactive free radical species.

Research Trends for Nanotoxicity Using Soil Nematode Caenorhabditis elegans (토양선충 Caenorhabditis elegans를 이용한 나노독성 연구동향)

  • Kim, Shin Woong;Lee, Woo-Mi;An, Youn-Joo
    • Journal of Korean Society of Environmental Engineers
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    • v.34 no.12
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    • pp.855-862
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    • 2012
  • Caenorhabditis elegans, a free-living nematode mainly found in the soil pore water, roles the critical function in trophic levels, energy flow, and decomposition in soil ecosystem. C. elegans is commonly used species to test soil toxicity. Recently, they are employed broadly as a test organism in nanotoxicology. In this study, a review of the toxicity of nanomaterials for C. elegans was presented based on SCI (E) papers. The nanotoxicity studies using C. elegans have been reported in 20 instances including the mechanism of toxicity. Most studies used K-medium, S-medium, and NGM (Nematode Growth Medium) plate as an exposure medium to test toxicity of nanoparticles. The effects observed include anti aging, phototoxicity, genotoxicity, and dermal effects on C. elegans exposed to nanoparticles. We found that the toxic mechanisms were related with various aspects such as lifespan abnormality, oxidative stress, distribution of particles on inter-organisms, and stress-related gene analysis. C. elegans has advantage to test toxicity of nanoparticles due to various cellular activities, full genome information, and easy observation of transparent body. C. elegans was considered to be a good test species to evaluate the nanotoxicity.