• 한국식품과학회지
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• 제29권3호
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• pp.588-594
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• 1997

제주산 온주밀감(Citrus unshiu)을 이용한 양조주를 제조하는데 적합한 효모를 선별하고, 밀감양조주의 품질에 직접적으로 연관된 효모의 citric acid 분해능에 대해서 기초조사를 실시하였다. 밀감양조주용으로 활용가능하다고 판단된 균주는 모두 11주이었다. 제주지역의 토양을 중심으로 5개의 효모균주를 분리, 선별하였는데, 그중 4개 주는 Saccharomyces cerevisiae, 나머지 1개 주는 S. ellipsoideus로 동정되었다. 한편 분양 균주 18주 중에서 선별된 균주는 6개 주로, S. cerevisiae 계통의 3개 주와 S. coreanus, S. uvarum, S. sake 각각 1개 주었다. 이중에서 S. cerevisiae 2개 균주의 citric acid 분해능을 조사하였다. Citric acid는 효모의 유일한 탄소원으로는 이용될 수 없었으나, citric acid가 glucose와 함께 탄소원으로 사용되었을 때, 배양액 중의 citric acid 농도는 감소하였으며, 정치배양 보다 진탕배양하였을 때 더 많이 감소하였다. 효모에 의한 citric acid의 이용은 ethanol 농도를 증가시키지 못하였으며, 오히려 citric acid는 효모의 생육을 부분적으로 저해하였다.

• 로봇학회논문지
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• 제6권3호
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• pp.255-262
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• 2011

Many intelligent robots have to be given environmental information to perform tasks. In this paper an intelligent robot, that is, a cleaning robot used a sensor fusing method of two sensors: LRF and StarGazer, and then was able to obtain the information. Throughout wall following using laser displacement sensor, LRF, the working area is built during the robot turn one cycle around the area. After the process of wall following, a path planning which is able to execute the work effectively is established using flow network algorithm. This paper describes an algorithm for minimal turning complete coverage path planning for intelligent robots. This algorithm divides the whole working area by cellular decomposition, and then provides the path planning among the cells employing flow networks. It also provides specific path planning inside each cell guaranteeing the minimal turning of the robots. The proposed algorithm is applied to two different working areas, and verified that it is an optimal path planning method.

• Journal of Electrical Engineering and Technology
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• 제7권6호
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• pp.1023-1033
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• 2012

An underwater planar covering problem is studied where the coverage region consists of polygonal cells, and line sweep motion is used for coverage. In many subsea applications, sidescan sonar has become a common tool, and the sidescan sonar data is meaningful only when the sonar is moving in a straight line. This work studies the optimal line sweep coverage where the sweep paths of the cells consist of straight lines and no turn is allowed inside the cell. An optimal line sweep coverage solution is presented when the line sweep path is parallel to an edge of the cell boundary. The total time to complete the coverage task is minimized. A unique contribution of this work is that the optimal sequence of cell visits is computed in addition to the optimal line sweep paths and the optimal cell decomposition.

• 한국전자파학회논문지
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• 제20권3호
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• pp.248-256
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• 2009

MIMO(Multiple-Input Multiple-Output) 시스템에서 EB(Eigen-Beamforming)는 MIMO 채 널의 특이 값 분해(Singular Value Decomposition: SVD)를 통하여 수신기의 유효 신호 대 잡음비(Signal-to-Interference Plus Noise Ratio: SI-NR)를 최대화하는 빔을 형성하는 방법으로써 널리 활용되고 있으나, 인접 셀 간섭 신호의 영향으로 셀 경계에 위치한 단말기의 신호 검출 성능은 급격히 열화되고 전송 효율은 감소하게 된다. 본 논문에서는 EB 전송을 활용하는 경우, 적응적 인접 셀 간섭 완화 방안을 제시하고 그 수신 성능을 평가한다. 특히, EB 전송을 이용하여 기지국예서 전송된 신호를 단말기가 수신할 때, 최대의 유효 신호 대 간섭 잡음비를 얻기 위한 OC(Optimum Combining) 및 MMSE-ISD(Minimum Mean-Squared Error for Intercell Spatial Demultiplexing)를 적응적으로 사용하기 위한 기준을 제시하고 유효 신호 대 간섭 잡음비 및 전송 용량 측면의 수신 성능을 분석한다. 제안하는 적응적 수신 방식은 수신 빔포밍 벡터만을 사용하는 기존의 EB 수신 방식 대비 평균 전송 용량 측면에서 향상된 성능을 보이며, 셀 경계 지역에 단말기가 위치할 경우 최대 2 bps/Hz 성능 개선을 가져온다.

• 한국통신학회논문지
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• 제29권9A호
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• pp.1022-1030
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• 2004

차세대 이동 및 우선통신 시스댐은 현재보나 얘우 큰 고속의 데이터 전송과 시스템 용량을 요구하고 였으며 이러한 요구를 충족시키기 위해서 복수의 안테나를 사용하여 송수신을 하는 MIMO(m 비 tiple input, multiple output) 기술이 널리 연구되고 있다 이 논문은 다수의 안테나를 가지고 있는 기지국, 소수의 안테나를 가지고 있는 단말기의 하향 링크에서 스케터령과 변동이 적으며 평탄한 페이딩 채널을 가정한다. 단영 사용가 MIMO 시스템에서 가지국이 채널상태정보를 가지고 있다연 SVD(singular value decomposition) 와 water filling 읍 사용 한 MIMO 기숭이 최대의 채널용량을 이룬다. 그러나 복수 사용자 시스템의 경우에는 공간분할 다중접속 기술을 이용하여, 보다 큰 전체 채널용량을 얻는 것이 가능하다 이 논문은 복수 사용가 시스템에서 각 사용자에게 복수 의 데이터 스트림을 전송하는 MIMO 공간분할 다중접속 기술을 제안한다. 제안하는 기술은 SVD 기반의 MIMO 기술이나 스마트 안테나를 사용한 공간분항 다중정속 기술보다 더 큰 전체 채널용량을 얻을 수 있다.

• Biomolecules & Therapeutics
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• 제12권1호
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• pp.25-33
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• 2004

In this study we investigated whether ATP loss was involved in the potentiated death of immunostimulated rat primary astrocytes in glucose-deprived condition. Rat primary astrocytes immunostimulated with LPS plus IFN-${\gamma}$ for 48 h underwent death upon glucose deprivation, which dependent on the production of peroxynitrite. Intracellular ATP level synergistically decreased by glucose deprivation in immunostimulated astrocytes but not in control cells, and the loss of ATP occurred well ahead of the LDH release. The synergistic cell death and ATP loss by immunostimulation and glucose deprivation were inhibited by iNOS inhibitor (L-NAME and L-NNA) or peroxynitrite decomposition catalyst (also a superoxide anion scavenger), Mn(III)tetrakis(N-methyl-4'-pyridyl)porphyrin (MnTMPyP). Exogenous addition of peroxynitrite generator, SIN-l timedependently induced ATP loss and cell death in the glucose-deprived astrocytes. Depletion of intracellular glutathione (GSH) and dis겨ption of mitochondrial transmembrane potential (MTP) were also observed under same conditions. Supply cellular ATP by the addition of exogenous adenosine or ATP during glucose deprivation inhibited ATP depletion, GSH depletion, MTP disruption and cell death in SIN-l treated or immunostimulated astrocytes. This study showed that perturbation in the regulation of intracellular ATP level in immunostimulated astrocytes might make them more vulnerable to energy challenging stimuli.

• 한국환경과학회지
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• 제9권3호
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• pp.267-273
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• 2000

In order to understand the impact for decomposition of blue-green algae Microcystis on water quality, the algae were cultivated with collection of natural population during approximately one month, when water-bloom of Microcystis dominated at August 31, 1999 in the lower part of the Okchon Stream. The enrichment of inorganic NㆍP nutrients didn't in algal assay and the effect of Microcystis on water duality was assessed from the variation of nutrients by algal senescence. Microcystis population seemed to play a temporary role of sink for nutrients in the water body. Initial algal density of Microcystis was 2.3×10/sup 6/ cells/㎖. When Microcystis population died out under light condition, algal NㆍP nutrients between 9∼12 days affected to increase of biomass after reuse by other algal growth as soon as release to the ambient water. However, cellular nutrients under dark condition were almost moved into the water during algal cultivation. NH₄, NO₃ and SRP concentration were highly increased with 160, 17 and 79 folds, respectively relative to the early. As a result, the senescence of Microcystis population seemed to be an important biological factor in which cause more eutrophy and increase of explosive algal development by a lot of nutrients transfer to water body. There are significantly observed an effort of reduce for production of inner organic matters such a phytoplankton as well as load pollutants from watershed in side of the water quality management of reservoir.

• 한국작물학회지
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• 제56권1호
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• pp.35-43
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• 2011

Thirty five isolates of Trichoderma species collected from seven different locations of Bangladesh were studied for morphological characters and molecular variation. Mycelial diameters of the isolates varied from 8.28 cm to 9.00 cm. Based on colony colour, isolates were grouped into five such as dark green, green, light green, yellowish green and whitish green. Maximum isolates were green and light green. On the basis of growth habit and colony consistency, the isolates were categorized into three groups, in which most species had fast growth and were compact in appearance. PCR-based Random Amplified Polymorphic DNA (RAPD) technique employing 3 decamer primers produced 36 scorable bands of which all (100%) were polymorphic. The co-efficient of gene differentiation (Gst) was 1.0000 reflecting the existence of high level of genetic diversity among the isolates. The Unweighted Pair Group Method of Arithmetic Means (UPGMA) dendrogram constructed from Nei's (1972) genetic distance produced 2 main clusters (13 isolates in cluster 1 and 22 isolates in cluster 2). The result indicating their genetic diversity has opened new possibility of using the most efficient and more isolates of Trichoderma in the preparation of biopesticide and decomposition of municipality waste.

• Toxicological Research
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• 제26권4호
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• pp.321-327
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• 2010

The ability of 80% ethanol extracts from five medicinal plants, Aralia continentalis, Paeonia suffruticosa, Magnolia denudata, Anemarrhena asphodeloides, and Schizonepeta tenuifolia, to neutralize hydroxyl radical, peroxyl radical and peroxynitrite was examined using the total oxyradical scavenging capacity (TOSC) assay. Peroxyl radical was generated from thermal homolysis of 2,2'-azobis(2-methylpropionamidine) dihydrochloride (ABAP); hydroxyl radical by an iron-ascorbate Fenton reaction; peroxynitrite by spontaneous decomposition of 3-morpholinosydnonimine N-ethylcarbamide (SIN-1). The oxidants generated react with $\alpha$-keto-$\gamma$-methiolbutyric acid (KMBA) to yield ethylene, and the TOSC of the substances tested is quantified from their ability to inhibit ethylene formation. Extracts from P. suffruticosa, M. denudata, and S. tenuifolia were determined to be potent peroxyl radical scavenging agents with a specific TOSC (sTOSC) being at least six-fold greater than that of glutathione (GSH). These three plants also showed sTOSCs toward peroxynitrite markedly greater than sTOSC of GSH, however, only P. suffruticosa revealed a significant hydroxyl radical scavenging capacity. Seven major active constituents isolated from P. suffruticosa, quercetin, (+)-catechin, methyl gallate, gallic acid, benzoic acid, benzoyl paeoniflorin and paeoniflorin, were determined for their antioxidant potential toward peroxynitrite, peroxyl and hydroxyl radicals. Quercetin, (+)-catechin, methyl gallate, and gallic acid exhibited sTOSCs 40~85 times greater than sTOSC of GSH. These four components also showed a peroxynitrite scavenging capacity higher than at least 10-fold of GSH. For antioxidant activity against hydroxyl radical, methyl gallate was greatest followed by gallic acid and quercetin. Further studies need to be conducted to substantiate the significance of scavenging a specific oxidant in the prevention of cellular injury and disease states caused by the reactive free radical species.

• 대한환경공학회지
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• 제34권12호
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• pp.855-862
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• 2012

Free-living 선충으로 알려진 Caenorhabditis elegans는 주로 토양 공극수에서 서식하며, 토양 영양단계, 에너지 흐름, 그리고 분해자로서 중요한 역할을 하는 것으로 알려져 있다. 최근 들어, C. elegans는 나노독성연구에 널리 사용되고 있는 추세이다. 본 연구에서는 C. elegans를 이용한 나노독성과 그에 대한 기작에 관련된 선행연구를 조사하였으며, 총 20건의 연구를 확인하였다. 대부분의 연구는 K-medium, S-medium, 그리고 NGM (Nematode Growth Medium) plate를 노출매체로 이용하고 있으며, 나노물질에 노출된 C. elegans로부터 관찰된 영향으로는 노화억제, 광독성영향, 유전독성, 그리고 표피자극 등이 포함되었다. C. elegans를 이용한 독성기작 연구는 개체 생활사 영향 평가, 산화스트레스 영향 평가, 유전독성영향 평가, 나노물질의 생체 내 분포, 그리고 나노물질 특성에 의한 독성영향 평가로 분류할 수 있다. 본 연구에서는 다양한 세포활성, 잘 알려진 유전정보, 그리고 투명한 구조로 인한 나노물질 관찰의 용이성을 바탕으로, C. elegans를 이용한 나노독성연구의 장점을 확인하였다. C. elegans는 나노독성을 평가하기에 적합한 시험종으로 고려되고 있다.