• Maxillofacial Plastic and Reconstructive Surgery
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• 제33권2호
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• pp.112-119
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• 2011

Purpose: Collagen membranes are used extensively as bioabsorbable barriers in guided bone regeneration. However, collagen has different effects on tissue restoration depending on the type, structure, degree of cross-linking and chemical treatment. The purpose of this study was to evaluate the inflammatory reaction, bone formation, and degradation of dehydrothermal treated porcine type I atelocollagen (CollaGuide$^{(R)}$) compared to of the non-crosslinked porcine type I, III collagen (BioGide$^{(R)}$) and the glutaldehyde cross-linked bovine type I collagen (BioMend$^{(R)}$) in surgically created bone defects in rat mandible. Methods: Bone defect model was based upon 3 mm sized full-thickness transcortical bone defects in the mandibular ramus of Sprague-Dawley rats. The defects were covered bucolingually with CollaGuide$^{(R)}$, BioMend$^{(R)}$, or BioGide$^{(R)}$ (n=12). For control, the defects were not covered by any membrane. Lymphocyte, multinucleated giant cell infiltration, bone formation over the defect area and membrane absorption were evaluated at 4 weeks postimplantation. For comparison of the membrane effect over the bone augmentation, rats received a bone graft plus different covering of membrane. A $3{\times}4$ mm sized block graft was harvested from the mandibular angle and was laid and stabilized with a microscrew on the naturally existing curvature of mandibular inferior border. After 10 weeks postimplantation, same histologic analysis were done. Results: In the defect model at 4 weeks post-implantation, the amount of new bone formed in defects was similar for all types of membrane. Bio-Gide$^{(R)}$ membranes induced significantly greater inflammatory response and membrane resorption than other two membranes; characterized by lymphocytes and multinucleated giant cells. At 10 weeks postoperatively, all membranes were completely resorbed. Conclusion: Dehydrotheramal treated cross-linked collagen was safe and effective in guiding bone regeneration in alveolar ridge defects and bone augmentation in rats, similar to BioGide$^{(R)}$ and BioMend$^{(R)}$, thus, could be clinically useful.

• 한국버섯학회지
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• 제20권3호
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• pp.178-182
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• 2022

버섯의 오랜 역사에도 불구하고 버섯의 유전적 기능과 분자유전학을 응용한 신품종 개발에 대한 연구는 크게 부족한 상황이다. 그러나 최근 유전자 가위인 CRISPR/Cas를 이용한 새로운 유전자 교정 기술이 개발됨에 따라 버섯 연구에서 이 기술을 이용한 다양한 시도가 이루어지고 있다. 특히 선택의 용이성을 위해 외래 유전자 삽입 없이도 고효율로 유전자 편집이 가능한 RNPs를 활용한 연구가 활발히 진행되고 있다. 그러나 RNPs는 원형질체의 세포막을 통과하기에 Cas9이 너무 거대하고 guide RNA가 쉽게 파괴된다는 단점을 가지고 있다. 이러한 단점을 극복하기 위하여 세포막 통과에 용이한 미네랄 성분인 CaP와 PAA를 조합하여 Nanoparticle을 형성함으로써 극복하고자 했다. 표고버섯 단핵 균주인 산조705-13을 이용하여 원형질체 분리에 적합한 Osmotic buffer를 찾기 위하여 0.6M과 1.2M의 Sucrose, Sorbitol, Mannitol, KCl을 처리하였고 그 결과 0.6M Sucrose가 가장 적합한 osmotic buffer임을 확인하였다. 또한 CaP으로 RNPs와 Nanoparticle 복합체를 형성하고 이 복합체가 RNase A로부터 RNPs의 기능을 온전히 보호하는 것을 확인할 수 있었다.

• 유기물자원화
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• 제21권1호
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• pp.39-44
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• 2013

본 연구에서는 수평 흐름 미생물 연료전지에서 유체 흐름이 전력 수율에 미치는 영향을 평가하고자 하였다. 본 연구에서는 산화전극 반응조의 바닥에 아크릴 막대를 설치하여 각각 4가지 유체의 흐름을 유도하였다. 각 반응조 형상에 따라 최대전력수율을 평가하였으며 추적자 실험을 통해 유체 흐름을 해석하였다. 분극 곡선 실험 결과 반응조별 최대 전력수율은 case 1, 2, 3 및 4에서 각각 95.7, 129.1, 190.9 및 $114.2mW/m^2$로 나타났다. 좌우 도류벽을 설치하여 S 형태의 유체 흐름을 유도한 case 3 반응조에서 가장 높은 전력이 생산되는 것으로 나타났다. 추적자 실험의 Morrill 분산지수 값에 따르면 case 4 반응조의 경우 반응조 전체에 기질이 골고루 분포하여 미생물 활성을 높일 수 있을 것으로 나타났다. 그러나 월류 현상에 의해 안정적인 운영을 할 수 없을 것으로 판단된다. 따라서 case 3 반응조의 경우 안정적인 운영 및 높은 전력수율을 얻을 수 있으므로 미생물 연료전지로 이용하기에 효과적일 것으로 나타났다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권17호
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• pp.7303-7307
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• 2014

Objective: To explore the clinical efficacy of gemcitabine concomitant with nedaplatin and drug resistance in the treatment of non-small cell lung cancer (NSCLC) and associated molecular predicators. Materials and Methods: A total of 68 patients diagnosed with NSCLC by histology served as the study objects and were randomly divided into an observation group treated with gemcitabine concomitant with nedaplatin and a control group with cisplatin concomitant with gemcitabine, 34 cases for each group. Short-term and long-term efficacies, adverse responses as well as the expression of nucleotide excision repair cross complementing 1 (ERCC1), ribonucleotide reductase subunit M1 (RRM1) and lung resistance-related protein (LRP) in NSCLC tissues in both groups were assessed. Results: The short-term objective response rate (ORR) and disease control rate (DCR) were 35.3% (12/34) and 76.5% (26/34) in the observation group and 38.2% (13/34) and 85.3% (29/34) in the control group, respectively, the differences not being statistically significant. The time to progression (TTP) in both groups were 1~12 months, while the median TTP was 135 d and 144 d, respectively. Though the survival was slightly higher in the control group, there were no significant differences in TTP and survival time. The rates of decreased hemoglobin, vomiting and nausea as well as renal toxicity were evidently lower in the observation group, while other adverse responses demonstrated no significant difference. The positive expression rates of ERCC1, RRM1 and LRP were 47.1% (16/34), 61.8% (21/34) and 64.7% (22/34) in the observation group, respectively. Compared with negative ERCC1 expression, ORR had decreasing trend and the overall survival time (OS) decreased significantly in patients with positive ERCC1 expression, which were markedly decreased by the positive expressions of RRM1 and LRP. Conclusions: Gemcitabine concomitant with nedaplatin has significant effects in the treatment of NSCLC, with an adverse response rate obviously lower than for cisplatin concomitant with gemcitabine, suggesting that wider use in the clinic is warranted. Additionally, the positive expressions of ERCC1, RRM1 and LRP may increase patient drug resistance, so they can be applied as the chemotherapeutic predicators to guide individualized therapy of NSCLC patients.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6493-6499
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• 2013

Background: Non-epithelial malignant ovarian tumors and clear cell carcinomas, Brenner tumors, transitional cell tumors, and carcinoid tumors of the ovary are rare ovarian tumors (ROTs). In this study, our aim was to determine the clinicopathological features of ROT patients and prognostic factors associated with survival. Materials and Methods: A total of 167 patients with ROT who underwent initial surgery were retrospectively analyzed. Prognostic factors that may influence the survival of patients were evaluated by univariate and multivariate analyses. Results: Of 167 patients, 75 (44.9%) were diagnosed with germ-cell tumors (GCT) and 68 (40.7%) with sex cord-stromal tumors (SCST); the remaining 24 had other rare ovarian histologies. Significant differences were found between ROT groups with respect to age at diagnosis, tumor localization, initial surgery type, tumor size, tumor grade, and FIGO stage. Three-year progression-free survival (PFS) rates and median PFS intervals for patients with other ROT were worse than those of patients with GCT and SCST (41.8% vs 79.6% vs 77.1% and 30.2 vs 72 vs 150 months, respectively; p=0.01). Moreover, the 3-year overall survival (OS) rates and median OS times for patients with both GCT and SCST were better as compared to patients with other ROT, but these differences were not statistically significant (87.7% vs 88.8% vs 73.9% and 170 vs 122 vs 91 months, respectively; p=0.20). In the univariate analysis, tumor localization (p<0.001), FIGO stage (p<0.001), and tumor grade (p=0.04) were significant prognostic factors for PFS. For OS, the univariate analysis indicated that tumor localization (p=0.01), FIGO stage (p=0.001), and recurrence (p<0.001) were important prognostic indicators. Multivariate analysis showed that FIGO stage for PFS (p=0.001, HR: 0.11) and the presence of recurrence (p=0.02, HR: 0.54) for OS were independent prognostic factors. Conclusions: ROTs should be evaluated separately from epithelial ovarian cancers because of their different biological features and natural history. Due to the rarity of these tumors, determination of relevant prognostic factors as a group may help as a guide for more appropriate adjuvant or recurrent therapies for ROTs.

• Tuberculosis and Respiratory Diseases
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• 제78권4호
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• pp.341-348
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• 2015

Background: There have been various results from studies concerning the predictors of recurrence in early-stage nonsmall cell lung cancer (NSCLC). Therefore, an accurate assessment is needed to guide effective adjuvant therapy. We investigated the predictors of a recurrence in patients with resected, early-stage NSCLC and the risk factors associated with locoregional or distant recurrence. Methods: This retrospective study was conducted on patients at the Pusan National University Hospital from January 2006 to December 2011. Patients with pathological stages I or II were included in this study, as based on the seventh edition TNM staging system. Multivariate Cox proportional hazard models were used to identify factors associated with recurrence. Results: Two hundred and forty-nine patients were included. Among them, 180 patients were stage I, and 69 were stage II. Overall, by multivariate analysis, the independent factors associated with a 5-year total recurrence were the presence of visceral pleural invasion (VPI) (p=0.018) and maximal standardized uptake values (SUVs) of tumors on positron emission tomography (PET) >4.5 (p=0.037). The VPI was the only independent risk factor associated with both locoregional and distant recurrence, in the analysis of the patterns of tumor recurrence and their risk factors. In the subgroup analysis of stage I patients, three variables (male, VPI and resection margin positive) were significantly associated with a 5-year recurrence. Conclusion: The independent factors associated with postoperative recurrence in early-stage NSCLC were as follows: PET SUV >4.5 and the presence of VPI. For patients with those factors adjuvant therapy should be recommended as a more efficacious treatment.

• 대한바이러스학회지
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• 제26권2호
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• pp.163-171
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• 1996

The recombinant pseudorabies virus major capsid protein (rMCP) was produced by expression of the MCP gene in Sf-9 cell using baculovirus transfer vector system. Following evaluation of the immunochemical properties of the rMCP, the immunogenicity of the recombinant subunit protiens were investigated in guinea pig and swine to obtain the preliminary guide line for the subunit vaccine using rMCP and gP50. It was proved that ultrasonication and 30% ammonium sulfate was most efficient to concentrate and purify the protein. The rMCP was safe in mice, guinea pigs and piglets. In guinea pigs, rMCP mixed with various adjuvants induced substantial degree of serum neutralizing antibody titers, but revealed incomplete protectivity against challenge. In swine, the combination of rMCP and gP50 showed the higher serum neutralizing antibody titers and cellular immune responses than rMCP alone. However, the protectivity was lower in comparison with the commercial gI-deleted inactivated vaccine. We expect these results to contribute to characterization of MCP gene of Korean isolate of PRV and to ultilize as preliminary information for prodution and evaluation of PRV recombinant subunit vaccines.

• 한국산학기술학회논문지
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• 제15권7호
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• pp.4528-4535
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• 2014

본 논문은 승객 위치정보 기반의 지하철 분산대피 안내 시스템을 제안하고자 한다. 최근 지하철 역사는 지하철 승강장 역할 뿐만 아니라, 환승, 쇼핑몰, 영화관 등의 다양한 편의 시설과 결합하여 대형화, 복잡화 되어 가고 있다. 이러한 지하철 역사의 복잡도 증가에 비해서 비상시를 대비한 대피경로, 대피안내 등의 대비체계는 부족한 실정이다. 이를 해결하기 위하여 현재 지하철에 설치되어 있는 Access Point(AP)와 실제 통계 자료를 이용하여 지하철 각 객차의 전체 승객 수를 추정하고, 추정된 승객 수와 지하철 역사의 출구 정보를 기반으로 각 객차의 승객 대피 경로를 방향 지시등과 스마트폰의 Push 알람을 통해 제공함으로써, 비상 시 가장 위험한 지하철 승객의 신속하고 안전한 대피시스템을 제안하였다. OpenWrt 임베디드 OS가 설치된 AP를 이용하여 접속자 수를 추정하는 실험을 수행하였으며, 시뮬레이션을 통해 승객 수를 추정한 결과 신뢰구간 파라미터 $1{\sigma}$, $2{\sigma}$, $3{\sigma}$에서 15%, 17% 그리고 23%의 평균 에러율을 갖는 것을 확인하였다. 이를 통해 본 시스템의 가능성을 확인하였다.

• 한국학교ㆍ지역보건교육학회지
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• 제12권3호
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• pp.1-15
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• 2011

Objectives: We describe the development of a smoking and drinking prevention program for adolescents, using intervention mapping. Methods: The study sample consisted of 1,000 high school second-grade students from 6 high schools in Seoul. The PRECEDE model was applied for the needs assessment. We carried out a social diagnosis by assessing the factors such as the quality of life, happiness level, and satisfaction with school life; an epidemiological diagnosis on the perceived health status, stress levels, and priority of health issues; a behavioral diagnosis on the smoking and drinking rate and the intention to smoke and drink; and an educational diagnosis on knowledge, beliefs, attitudes, self-efficacy, outcome expectations, social norms and life skills. Results: The development process included a needs assessment, identifying factors that influence smoking and drinking among adolescents. Intention, knowledge, perceived norms, perceived benefit, perceived cost, perceived susceptibility, self-efficacy, and life skills were identified as determinants. Three performance objectives were formulated to describe what an individual needs to do in order to avoid smoking and drinking. Subsequently, we constructed an intervention matrix by crossing the performance objectives with the selected determinants. Each cell describes the learning objectives of the smoking and drinking prevention program. The program used methods from the transtheoretical model, such as consciousness raising, outcome expectations, self-reevaluation, self-liberation, counterconditioning, environmental reevaluation, and stimulus control. The program deals with the effects of smoking and drinking, self-improvement, decision making, understanding advertisements, communication skills, social relationships, and assertiveness. Conclusions: By using the process of intervention mapping, the program developer was able to ensure a systematical incorporation of empirical and new data and theories to guide the intervention design. Programs targeting other health-related behavior and other methods or strategies can also be developed using this intervention mapping process.

• 한국HCI학회논문지
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• 제6권1호
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• pp.17-20
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• 2011

Fred.E Fiedler는 상황 리더쉽 이론에서 사회 문화적 특성에 따른 Work-style을 근거하여 문화집단을 과업지향적인(Task-oriented) 집단과 관계지향적인 (Relationship-oriented)집단으로 나눈다. 본 연구는 제품사용에 있어 Task를 수행하는 성향의 정도가 제품 인터렉션에 영향을 미칠 것이라는 가설을 세우고, 이를 검증하기 위하여 Fred.E Fiedler의 상황 리더쉽 이론을 근거로 하는 LPC 실험결과의 성향 정도에 따른 제품 사용 인터렉션을 연구한다. Task-oriented 성향과 가까운 문화권 집단은 제품사용에 있어 의도한 목적을 단 시간 빠르게 수행(usability)하는 퍼포먼스의 인터렉션을 중시하고, Relationship-oriented 성향과 가까운 문화권 집단은 제품사용에 있어 목적을 수행(usability)할 때 의미부여(refelctive)적인 측면을 더 중요하게 생각할 것이라는 가설을 증명하기 위한 실험으로 비교적 Task의 의미부여(refelctive)가 동등하고 빈번하게 잘 일어나는 기기인 모바일 단말기 사용에 있어 이 두 집단의 주소록의 주기능과 부기능 사용행태를 비교하고 데이터를 수집, 분석하여 가설을 검증한다. 이 연구결과, 주소록 사용에 있어서 피들러의 상황이론에 의한 Task 수행성향은 모바일 단말기 인터렉션 사용에 영향과의 연관성은 다소 낮게 나타났으며, 관련하여 본 실험 도중 몇 가지 흥미로운 패턴을 발견할 수 있었는데, 이를 기반으로 향후 모바일 단말기의 주소록의 사용자 지향적인 디자인 방향성에 대한 가이드라인을 제시하였다.