• Title/Summary/Keyword: Cell wall structure

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Growth and Histological Characteristics of Barley (Hordium vulgare L.) Seedling to NaCl Stress (NaCl Stress에 따른 보리 유묘의 생육특성 및 세포학적 반응)

  • Cho, Jin-Woong;Kim, Choong-Soo;Lee, Sok-Young;Park, Ki-Sun
    • Korean Journal of Environmental Agriculture
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    • v.17 no.4
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    • pp.335-340
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    • 1998
  • This study was conducted to determine the morphological responses of barley seedlings to NaCl stress and to investigate histological changes of cells with transmission electron microscope(TEM) after NaCl stress. Plant height and root length of 10-day old barley seedlings with NaCl stress were reduced and inhibition level was found to be more severe in the plant height than in the root length. The leaf length, leaf width and leaf area were shorter as well with NaCl stress than without NaCl stress. However, there was no difference in the number of roots between NaCl treatments. The weight of dry matter decreased at higher NaCl concentrations, especially at 100mM NaCl. The water content of shoots tend to decrease at higher NaCl concentrations, but there was no difference in the water content of roots, The reduced sugar content was greatly increase than starch. Cellulose content was higher in NaCl stressed-plant than control, and tended to decreased at higher NaCl concentrations. Lignin content also decreased NaCl stressed-plant but there was no tendency at NaCl stress concentrations. Electric conductivity of cell sap with seedlings was high with NaCl stressed-plant. Amount of cell sap gradually increased with time in the roots than in the shoots, The grana of chloroplasts was changed by 150mM NaCl concentration. The christe of mitochondria in root meristematic sells ruined in structure and cell wall of leaf and root was also ruined by NaCl stress.

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Analysis of Temperature and Power Generation Characteristics of Bifacial BIPV System Applied into Curtain Wall (양면형 BIPV 시스템의 커튼월 적용에 따른 온도 및 발전특성 분석)

  • Kang, Jun-Gu;Kim, Yong-Jae;Kim, Jun-Tae
    • Journal of the Korean Solar Energy Society
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    • v.35 no.4
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    • pp.57-66
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    • 2015
  • BIPV system not only produces electricity at building, but also acts as a material for building envelope. Thus, it can increase the economical efficiency of PV system by saving the cost for building materials. Bifacial solar cell can convert solar energy to electrical energy from both sides of the cell. In addition, it is designed as 3 busbar layout which is the same with ordinary mono-facial solar cells. Therefore, many of the module manufacturers can easily use the bifacial solar cells without changing their manufacturing equipments. Moreover, bifacial PV system has much potential in building application by utilizing glass-to-glass structure of PV module. However, the electrical generation of the bifacial PV module depends on the characteristics of the building surface which faces the module, as well as outdoor environment. Therefore, in order to apply the bifacial PV module to building envelope as BIPV system, its power generation characteristics are carefully evaluated. For this purpose this study focused on the electrical performance of the bifacial BIPV system through the comparative outdoor experiments. As a result, the power generation performance of the bifacial BIPV system was improved by up to 21% compared to that of the monofacial BIPV system. Therefore, it is claimed that the bifacial BIPV system can replace the conventional BIPV system to improve the PV power generation in buildings.

Study on Immuno-stimulating Activity of ${\beta}$-Glucan Isolated from the Cell Wall of Yeast Mutant Saccharomyces cerevisiae IS2 (효모변이주 Saccharomyces cerevisiae IS2 세포벽 유래의 베타글루칸 면역활성능에 관한 연구)

  • Park, Jeong-Hoon;Kang, Man-Sik;Kim, Hong-Il;Chung, Bong-Hyun;Lee, Kwang-Ho;Moon, Won-Kuk
    • Korean Journal of Food Science and Technology
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    • v.35 no.3
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    • pp.488-492
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    • 2003
  • Yeast cell wall mutant, Saccharomyces cerevisiae IS2 was screened by the NTG treatment of Saccharomyces cerevisiae KCTC 7911. The mutant was highly resistant to zymolase, which specifically degrades ${\beta}$-1,3-D-glucose chain of ${\beta}$-glucan and mechanical disruption by glass beads. These phenomena demonstrate that the yeast mutant has cell wall structure different from the wild-type. The ${\beta}$-glucan of yeast mutant and wild-type strains was recovered by sequential extraction with NaOH. The injection of ${\beta}$-glucan into the abdominal cavity of mouse resulted in an increase in the number of peritoneal immune cells, NO (nitric oxide) production, and phagocytic activity of macrophage. The number of immune cells was found to be $3.90{\times}10^6\;cells/10\;mL$ and $5.48{\times}10^6\;cells/10\;mL$ with the wild-type and mutant ${\beta}$-glucan, respectively. The effect on the NO production and phagocytic activity of mutant ${\beta}$-glucan were 1.69 and 1.43-fold higher than those of wild-type. These results indicate that the immuno-stimulating activity of alternated ${\beta}$-glucan from mutant yeast is higher than that of wild-type.

Zygosaccharomyces rouxii Combats Salt Stress by Maintaining Cell Membrane Structure and Functionality

  • Wang, Dingkang;Zhang, Min;Huang, Jun;Zhou, Rongqing;Jin, Yao;Wu, Chongde
    • Journal of Microbiology and Biotechnology
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    • v.30 no.1
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    • pp.62-70
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    • 2020
  • Zygosaccharomyces rouxii is an important yeast that is required in the food fermentation process due to its high salt tolerance. In this study, the responses and resistance strategies of Z. rouxii against salt stress were investigated by performing physiological analysis at membrane level. The results showed that under salt stress, cell integrity was destroyed, and the cell wall was ruptured, which was accompanied by intracellular substance spillover. With an increase of salt concentrations, intracellular Na+ content increased slightly, whereas intracellular K+ content decreased significantly, which caused the increase of the intracellular Na+/K+ ratio. In addition, in response to salt stress, the activity of Na+/K+-ATPase increased from 0.54 to 2.14 μmol/mg protein, and the ergosterol content increased to 2.42-fold to maintain membrane stability. Analysis of cell membrane fluidity and fatty acid composition showed that cell membrane fluidity decreased and unsaturated fatty acid proportions increased, leading to a 101.21% rise in the unsaturated/saturated fatty acid ratio. The results presented in this study offer guidance in understanding the salt tolerance mechanism of Z. rouxii, and in developing new strategies to increase the industrial utilization of this species under salt stress.

Numerical Analysis of Incompressible and Compressible Flow Around a Butterfly Valve (버터플라이 벨브 주위의 비압축성 및 압축성유동 특성에 대한 수치해석)

  • 이종욱;이두환;최윤호
    • Journal of Energy Engineering
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    • v.11 no.1
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    • pp.26-33
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    • 2002
  • In this paper, incompressible and compressible flow characteristics around the butterfly valve have been investigated. In order to simplify the problem, a flat disk valve with various valve disk angles and pressure ratios is considered in the present calculations. It was found that as the disk angle increases, the stagnation point on the front surface of the disk moves to the center of the surface and the inflow velocity decreases. The maximum flow velocity occurs at the downstream of throat because of the formation of vents contracta. As the pressure ratio decreases, compressibility effects increase and the jet formed between the throttle body wall and the disk edge becomes supersonic. This flow also builds up as a shock cell structure. The increase of disk angle and pressure ratio makes the mass flow at the inlet decrease, while the increase of disk angle and the decrease of pressure ratio make the pressure loss coefficient increase.

Effects of Condensation Heat Transfer Model in Calculation for KNGR Containment Pressure and Temperature Response

  • Eoh, Jae-Hyuk;Park, Shane;Jeun, Gyoo-Dong;Kim, Moo-Hwan
    • Nuclear Engineering and Technology
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    • v.33 no.2
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    • pp.241-253
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    • 2001
  • Under severe accidents, the pressure and temperature response has an important role for the integrity of a nuclear power plant containment. The history of the pressure and temperature is characterized by the amount and state of steam/air mixture in a containment. Recently, the heat transfer rate to the structure surface is supposed to be increased by the wavy interface formed on condensate film. However, in the calculation by using CONTAIN code, the condensation heat transfer on a containment wall is calculated by assuming the smooth interface and has a tendency to be underestimated for safety. In order to obtain the best- estimate heat transfer calculation, we investigated the condensation heat transfer model in CONTAIN 1.2 code and adopted the new forced convection correlation which is considering wavy interface. By using the film tracking model in CONTAIN 1.2 code, the condensate film is treated to consider the effect of wavy interface. And also, it was carried out to investigate the effect of the different cell modelings - 5-cell and 10-cell modeling - for KNGR(Korean Next Generation Reactor) containment phenomena during a severe accident. The effect of wavy interface on condensate film appears to cause the decrease of peak temperature and pressure response . In order to obtain more adequate results, the proper cell modeling was required to consider the proper flow of steam/air mixture.

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Ultrastructural and Cytochemical Studies on Root Hair Cells of Ginseng(Panax ginseng C.A. Meyer) (인삼(人蔘)(Panax ginseng C.A Meyer) 근모세포(根毛細胞)의 미세구조(微細構造) 및 (세포화학적)細胞化學的 연구(硏究))

  • Jeong, B.K.;Kim, W.K.
    • Applied Microscopy
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    • v.15 no.2
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    • pp.69-79
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    • 1985
  • Ultrastructural and cytochemical studies of the root hair cell and the trichoblast were undertaken with light and electron microscopes to clarify the type of root hair, fine structure and the activities of acid phosphatase and ATPase. The root hair was differentiated from the middle portion of the cell, and perpendicularly to the long axis of the cell. Consequently, the type of root hair comes under the panicoid type. In the trichoblast, nucleus and cytoplasm are located in the vicinity of cortex. On the contrary, after the root hair is formed, they migrate to the apical region of the root hair, and the basal region of the root hair is filled with numerous vacuoles. Cell walls of actively growing root hairs are subdivided into two layers on the basis of the arrangement of cellulose microfibrils. New cell wall of the root hair is presumptively formed from Golgi complex-derived vesicles. Activity of acid phosphatase appeared on tonoplast, plasma membrane, and nuclear envelope, whereas ATPase activity appeared on the plasma membrane, heterochromatin, and mitochondrial cristae.

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Capillary Flow in Different Cells of Metasequoia glyptostroboides, Anthocephalus cadamba, and Fraxinus rhynchophylla (메타세콰이어, 카담, 물푸레나무 세포내강의 액체이동)

  • Chun, Su Kyoung
    • Journal of the Korea Furniture Society
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    • v.29 no.1
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    • pp.1-7
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    • 2018
  • A study was carried out to observe the 1% aqueous safranine solution flow speed in longitudinal and radial directions of softwood Metasequoia glyptostroboides, diffuse-porous wood Anthocephalus cadamba and ring-porouswood Fraxinus rhynchophylla. In radial direction, ray cells and in longitudinal direction, tracheids, vessel and wood fiber were considered for the measurement of liquid penetration speed at less than 12% moisture contents (MC). The length, lumen diameter, pit diameter, end wall pit diameter and the numbers of end wall pits determined for the flow rate. The liquid flow in the those cells was captured via video and the capillary flow rate in the ones were measured. Vessel in hardwood species and tracheids in softwood was found to facilitate prime role in longitudinal penetration. Anatomical features like the length and diameter, end-wall pit numbers of ray parenchyma were found also responsible fluid flow differences. On the other hand, vessel and fiber structure affected the longitudinal flow of liquids. Therefore, the average liquid penetration depth in longitudinal tracheids of Metasequoia glyptostroboides was found the highest among all cells considered in Anthocephalus cadamba and Fraxinus rhynchophylla In radial direction, ray parenchyma of Metasequoia glyptostroboides was found the highest depth and the one of Fraxinus rhynchophylla was the lowest. The solution was penetrated lowest depth in the wood fiber of Fraxinus rhynchophylla. The large vessel of Fraxinus rhynchophylla was found the lowest depth among the vessels. The solutin was penetrated to the wood fiber of Anthocephalus cadamba higher than the one of Fraxinus rhynchophylla.

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Capillary Flow in Different Cells of Larix Kaempferi, Betula Davurica, Castanea crenata (일본잎갈나무, 물박달나무, 밤나무 세포내강의 액체이동)

  • Chun, Su Kyoung
    • Journal of the Korea Furniture Society
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    • v.28 no.1
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    • pp.88-93
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    • 2017
  • A study was carried out to observe the 1% aqueous safranine solution flow speed in longitudinal and radial directions of softwood Larix kaempferi (Lamb.)Carriere, diffuse-porous wood Betula davurica Pall.. and ring-porouswood Castanea crenata S.etZ. In radial direction, ray cells and in longitudinal direction, tracheids, vessel and wood fiber were considered for the measurement of liquid penetration speed at less than 12% moisture contents (MC). The length, lumen diameter, pit diameter, end wall pit diameter and the numbers of end wall pits determined for the flow rate. The liquid flow in the those cells was captured via video and the capillary flow rate in the ones were measured. Vessel in hardwood species and tracheids in softwood was found to facilitate prime role in longitudinal penetration. Anatomical features like the length and diameter, end-wall pit numbers of ray parenchyma were found also responsible fluid flow differences. On the other hand, vessel and fiber structure affected the longitudinal flow of liquids. Therefore, the average liquid penetration depth in longitudinal tracheids of Larix kaempferi was found the highest among all cells considered in Betula davurica and Castanea crenata, In radial direction, ray parenchyma of Larix kaempferi was found the highest depth and the one of Betula davurica was the lowest. The solution was penetrated lowest depth in the wood fiber of Castanea crenata. The large vessel of Castanea crenata was found the lowest depth among the vessels. The solutin was penetrated to the wood fiber of Betula davurica higher than the one of Castanea crenata.

Lignin Degradation of Pine Wood by Unidentified Decay Fungi and Observation by Scanning Electron Microscope (미동정 부후균에 의한 소나무재의 Lignin 분해와 주사전자현미경(SEM)을 이용한 관찰)

  • Park, Heon;Min, Kyeong-Heui
    • Journal of the Korean Wood Science and Technology
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    • v.31 no.4
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    • pp.71-80
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    • 2003
  • The lignin degrading fungi were isolated from decayed woods and fruiting bodies gathered in forest area. Lignin degradation ability was investigated by Klason lignin of microbial treated pine wood. Among selected fungi, CJ-6 had 49.48% Klason lignin loss which was greater than 40.58% shown by Trametes versicolor that it is known as a typical lignin degrading fungus. Also, the biodegradation process and morphological features of degraded pine wood by selected fungi were observed with the scanning electron microscope. At the stage of 20 days incubation, mycelia invasion was observed without any failure of wood structure. At 60 days, wood decay was gone in some degree and one part of tracheid and ray wall was destroyed. At 100 days, tracheid wall was severely destroyed, and distinction between ray cell was difficult as cell wall was decayed much.