• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1265-1265
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• 2001

The organic materials included in excreta of livestock are important resources for organic manure and for improving soil quality, although there is still far from effective using. One reason for this is still unclearly standard of quality for evaluation of manure made from excreta of livestock. Therefore, the objective of this study is to develop rapid and accurate analytical method for analyzing organic compositions of manure made from excreta of livestock, and to establish quality evaluation method based on the compositions predicted by near infrared reflectance spectroscopy (NIRS). Sixteen samples of manure, each eight samples prepared from two treatments, were used in this study. The manure samples were prepared by mixing 560 kg feces of swine,60 kg sawdust with moisture content was adjusted to be 65%. The mixture was then keep under two kinds of shelter, black and clear sheets, as a treatment on the effect of sunlight. Samples were taken in every week (form week-0 to 7) during the process of manure making. Samples were analyzed to determine neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) by detergent methods, and organic cell wall (OCW) and fibrous content of low digestibility in OCW (Ob) by enzymatic methods. Biological oxygen demand (BOD) was analyzed by coulometric respirometer method. These compositions were carbohydrateds and lignin that were hardly digested. Spectra of samples were scanned by NIR instrument model 6500 (Pacific Scientific) and read over the range of wavelength between 400 and 2500nm. Calibration equations were developed using eight manure samples collected from black sheet shelter, while prediction was conducted to the other eight samples from clear sheet shelter. Accuracy of NTRS prediction was evaluated by correlation coefficients (r), standard error of prediction (SEP) and ration of standard deviation of reference data in prediction sample set to SEP (RPD). The r, SEP and RPD value of forage were 0.99, 0.69 and 7.6 for ADL, 0.96, 1.03 and 4.1 for NDF, 0.98, 0.60 and 4.9 for ADF, 0.92, 1.24 and 2.6 for Ob, and 0.91, 1.02 and 7.3 for BOD, respectively. The results indicated that NIRS could be used to measure the organic composition of forage used in manure samples.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.545-551
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• 2010

The primary objective of this study was to determine the relationship between fruit quality characteristics and pithiness fruit occurrence for confirming occurrence factors of pithiness tissues in 'Niitaka' pears ($Pyrus$ $pyrifolia$ Nakai). Fruits were harvested 4 times in 1 week intervals from 160 days after full bloom (DAFB) to 181 DAFB. Harvested fruits were separated with the specific gravity by Archimedes laws. Also, with specific gravity level in pear fruit, sugar content, acidity, and texture profile analysis (TPA) were investigated. Final verifiability of pithiness fruit was approved by eye observation at 90 days after storage. Also, in ASS (Alcohol Soluble Solid) and AIS (Alcohol Insoluble Solid), calcium contents were investigated. Although the differences in sugar and organic acid content were not significant in fruits divided by gravity, flesh hardness was depressed in fruits that showed low gravity. These results were more conspicuous in late harvested fruits (at 174 and 181days after full bloom) which showed low gravity, representing less content of AIS content and calcium in ASS and AIS when compared with early harvest fruits. Fruits of lower gravity below 1.0 measured with dipping induced the progression of pithiness symptom up to 76%. Consequently, harvest timing is a more concise factor in pithiness fruit occurrence although those symptom also affected by storage condition. Furthermore, the cause of pithiness fruit occurrence by late harvest is due to the lower cell wall binding calcium.

• Microbiology and Biotechnology Letters
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• v.20 no.6
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• pp.625-629
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• 1992

This work was carried out to study physiological characteristics of Rhodosporidium toru[oides cells having two different mating types. The mating type A produces internal. cell wall-bound, and external invertases while type a produces only two invertases except external invertase. Comparing their characteristics after partial purification of internal invertases from both mating type cells, invertase from type a has decreased 15% of invertase activity only by $Mn^{2+}$ I while invertase from type A has been increased 11% of invertase activity by $Zn^{2+}$ and decreased 15% of invertase activity by $Mn^{2+}$ On the effect of enzyme inhibitor, invertase of type a was inhibited from 12% to 57% by 2-mercaptoethanol, sodium dodecyl sulfate, phenol. but invertase of type A was slightly inhibited only by phenol. The thermal stability of both invertases has showed steep inactivation at above $80^{\circ}C$ and their optimal temperatures were similar at $60^{\circ}C$ . Invertase from type A showed stability only on condition of acid from pH 3 to 6 and its opimal pH was 5.0, while invertase from type a showed stability at the wide range of pH 3-10 and its optimal pH was 4.0. And the $K_m$ values of invertases from type A and type a were $2.5{\times}10^3$M and$3.4{\times}10^3$M, respectively.

• Microbiology and Biotechnology Letters
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• v.43 no.2
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• pp.126-133
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• 2015

Ten types of farm-made brewing vinegars were collected and four high acetic acid-producing strains (CV1, CV3, CV5, and CV6) were isolated. Among them strain CV1, exhibiting highly alcohol-resistant and acetic acid-producing properties, was selected and its taxonomic properties were investigated by phenotypic (particularly chemotaxonomic) characterization and phylogenetic inference based on 16S rRNA gene sequence analysis. On SM broth agar, cells of strain CV1 were gram-stainingnegative and formed pale white colonies with smooth to rough surfaces. Strain CV1 produced acetate from ethanol and was resistant to up to 8% (v/v) ethanol in LM broth. Strain CV1 had a G+C content of 61.0 mol%, contained meso-DAP as the cell wall amino acid, and possessed Q-10 as the major ubiquinone. A comparison of 16S rRNA gene sequences showed that strain CV1 was most closely related to Gluconacetobacter saccharivorans (≥99.0% identity). In liquid media, the optimum growth conditions for acetic acid production were 30℃ and pH >3.0 and strain CV1 produced 9.3% and 8.4% acetic acids from 10% and 9% alcohol concentrations, respectively.

• Korean Journal of Animal Reproduction
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• v.18 no.2
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• pp.141-150
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• 1994

Morphological features of the interaction between the hatching blastocyst and implantation in pig were studied by electron microscopy. The observations extended from late blastocyst stage to the completion of trophoblastic erosion of the epithelium and early decidual transformation of the epithelium and early decidual transformation of the stromal cells. Between day 7 and 17 of pregnancy, blastocysts from 0.3 to 12 mm in diameter were flushed from the uterine horns of Dutch Landrace pigs. On the 7th of development in the pig blastocyst, the blastocyst shedded of the zona pellucida established the tips of microvilli and with bleb-like cytoplasmic protrusions of the epithelial cells. From day 11 on in pig embryo, the bilayered trophoblast undergoes a dramatic phase of elongation so that the initially spherical expanded blastocyst becomes tubular. In pig, close apposition to the uterine wall beg-ins at about 12 $^1$/$_2$ days and then attachment occurred during the afternoon of the 16th or 18th day post coitum. At this stage, embryonic loss compared with corpus luteum number is up to 40% of ovulated oocytes. Therefore, the implantation failture of these embryos may be mainly caused by morphological abnormality and failture of zona shedding.

• Journal of the Korea Organic Resources Recycling Association
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• v.14 no.3
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• pp.77-84
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• 2006

The objective of this research is to evaluate various reclamation methods of sewage sludge cake after treating with microwave under optimum conditions. In this study the sewage sludge cakes used from S and N wastewater treatment plants in the P city. Microwave with an induced electricity heating way was employed for dehydration of sewage sludge cake. Microwave operation conditions is 2,450 MHz of frequency and the power with 1 to 4 kW. This sewage sludge cake had a moisture content of 70%. The moisture content of the sludge decreased notable up to 2%(wt) resulted in breaking of cell wall. When the treated sewage sludge cake mixed with soils could be applied to use midterm and last cover material soils. Moreover, the adsorption ability of heavy metals such as copper, lead, chromium and cadmium was greatly enhanced by treated sewage sludge cake. Within 30 minutes, 1ppm of copper, chromium and cadmium and 10ppm of lead with 1g of the treated sewage sludge cake in $100m{\ell}$ were below detection. It was possible to use the treated sewage sludge cake as an absorbent for absorption of toxic heavy metals. Results from this research indicated that using of microwave radiation was an effective method for treating sewage sludge cake economically and environmental. A point of view of reclamation, the treated sewage sludge cake appeared to be feasible with an adsorption of heavy metals in steady of using expensive yellow earth.

• Journal of Korean Society of Environmental Engineers
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• v.39 no.7
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• pp.418-425
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• 2017

ZnO nanoparticles (ZnO NPs) are mainly used in semiconductors, solar cells, biosensors, and cosmetics (sunscreen). In this study, we investigated the behavior of ZnO NPs in aquatic and soil environments and their effects on plants (Artemisia annua L.) in hydroponic cultivation. It was confirmed that the ZnO NPs size increased and their dissolution decreased with increasing in pH. Leaching distance of ZnO NPs was less than 2.5 cm, indicating that ZnO NPs had a little potential to leach into deeper soil layers. When ZnO NPs were exposed to plant, the total weights of plants decreased. The effects on the length of root and shoot were not observed. In addition large amount of ZnO NPs were adsorbed on the surface of plant root and didn't translocate into shoot. These results suggest that ZnO NPs block the pores of the root cell wall and decrease the bioavailability of plant nutrients. Therefore it can be speculated that the particles increase in size and settle down in the water environment and may adversely affect the plant growth by firmly adhering to the root surface when the ZnO NPs are exposed to the environment.

• Journal of Pharmaceutical Investigation
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• v.32 no.3
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• pp.215-221
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• 2002

Cephradine is a first generation cephalosporin and has broad spectrum antibacterial activity against gram-positive and gram-negative microorganisms, through inhibition of bacterial cell wall synthesis. Cephradine is useful for treatment of infections of the urinary and respiratory tract, skin and soft tissues. The purpose of the present study was to evaluate the bioequivalence of two cephradine capsules, Cefradine Yuhan (YuHan Corporation) and Broadcef (Ilsung Pharmaceuticals Co. Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). The cephradine release from the two cephradine capsules in vitro was tested using KP VII Apparatus II method with various different kinds of dissolution media (pH 1.2, 4.0, 6.8 buffer solution and water). Twenty normal male volunteers, $23.10{\pm}2.90$ years in age and $67.69{\pm}8.04\;kg$ in body weight, were divided into two groups and a randomized $2{\times}2$ cross-over study was employed. After one capsule containing 500 mg as cephradine was orally administered, blood was taken at predetermined time intervals and the concentrations of cephradine in serum were determined using HPLC method with UV detector. The dissolution profiles of two cephradine capsules were very similar at all dissolution media. Besides, the pharmacokinetic parameters such as $AVC_t,\;C_{max}\;and\;T_{max}$ were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed $AVC_t\;and\;C_{max}$ and untransformed $T_{max}$. The results showed that the differences in $AVC_t,\;C_{max}\;and\;T_{max}$ between two capsules based on the Cefradine Yuhan were -2.87%, -0.96% and -4.85%, respectively. There were no sequence effects between two capsules in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of 1og(0.8) to log(1.25) $(e.g.,\;log(0.93){\sim}log(1.02)\;and\;log(0.88){\sim}log(1.13)\;for \;AVC_t\;and\;C_{max},\;respectively)$. The 90% confidence interval using untransformed data was within ${\pm}20%$ $(e.g., \;-17.54{\sim}7.78\;for\;T_{max})$. All parameters met the criteria of KFDA guideline for bioequivalence, indicating that Broadcef capsule is bioequivalent to Cefradine Yuhan capsule.

• Current Research on Agriculture and Life Sciences
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• v.3
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• pp.92-98
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• 1985

The experiments were conducted to investigate the activity changes of peroxidase, existence of isoenzyme and the changes of reserved substances in tomato under subatmospheric pressure storage condition. The results obtained were as follows: 1. Soluble fraction showed the highest peroxidase activity and followed by cell wall fraction, mitochondria fraction and ribosome fraction in that order. 2. Peroxidase activity was decreased during the ripening and senescence period in tomato. Especially, peroxidase activity in tomato was higher at a room temperature ($25^{\circ}C$) than at a low temperature ($15^{\circ}C$). The decreasing inclination was similar in both treatment. The peroxidase activity was higher in 380 Torr, than in 570 Torr. 3. At least, two isoperoxidases(Soluble or solubilized) were identified from different extraction procedures. Three of four isoenzymes were recognized from a vertical slab of polyacrylamide gel electrophoresis. 4. The changes of components in tomato under SAP were generally affected by temperature and pressure. Especially, quality of tomato stored at a low temperature ($15^{\circ}C$) and SAP (380 Torr.) was best during storage.

• Applied Biological Chemistry
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• v.38 no.4
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• pp.313-319
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• 1995

The involvement of the cell-wall degrading enzymes in Rhizobium has long been an unsolved question about the infection process in the formation of root nodule. To assess the contribution of the cellulase to the nodulation of rhzobia, here we report the production of cellulase from R. meliloti TAL1372 which degrade carboxymethylcellulose (CMC) model substrate with CMC-plate method. We constructed a genomic library by cloning Sau3A-digested genomic DNA from R. meliloti TAL1372 into the BamHI site of the cosmid vector pLAFR3. Out of more than one thousand transductants of E. coli, one clone (pRC8-71) had CM-cellulase activity and contained pLAFR3 cosmid with 30 kb insert of R. meliloti DNA The product of CM-cellulase gene was analyzed by native PAGE. About 45 kD protein was considered to be a product of the gene. Tn5 mutagenesis reveals that the structural gene located in a ca. 3 kb KpnI fragment. The cellulase-minus mutants of R. meliloti TAL1372 were obtained by Tn5 mutagenesis of pRC8-71 and marker exchange techniques. Analyses of the nodulation ability of these Tn5 mutants showed that the CM-cellulase gene of R. meliloti TAL1372 may be involved in early nodulation development on alfalfa (Medicago satiua).