• Transactions of the Korean Society of Mechanical Engineers A
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• v.26 no.2
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• pp.387-398
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• 2002

The impact response and damage of CLAS panel was investigated experimentally. The facesheet material used was RO4003 woven-glass hydrocarbon/ceramic and the core material was Nomex honeycomb with a cell size of 3.2mm and a density of 96 kg/㎥. The shield plane used was RO4003 and 2024-T3 aluminum. Static indentation and impact test was conducted to characterize the type and extent of the damage observed in two CLAS panels, and the performance of antenna used in a wireless LAN system. Correlation of peak contact force, residual indentation and the delamination area shows impact damage of the panel with an aluminum shield plane is larger than that of the panel with RO4003 shield plane, although the former is more penetration resistant. The damage was observed by naked eye, ultrasonic inspection and cross sectioning. The shape and size of delamination was estimated by ultrasonic inspection, and the area of delamination linearly increases as impact energy increases. The performance of impact damaged antenna was estimated by measuring return loss and radiation pattern. It was revealed that the performance of antenna was related to the impact damage and there was a threshold that the performance of antenna fell as impact energy level changed. The threshold was between the impact energies of 1.5J and 1.75J.

• Applied Microscopy
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• v.30 no.3
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• pp.255-264
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• 2000

The topographic correlation between the processes of photoreceptor cells and nerve cells in the pineal organ of catfish, Parasiluns asotus, was studied using 3D electron microscopy. Upon examination, one neuronal cell process was found to pass through the intertwined processes of the photoreceptor cells. Interestingly , we observed two photoreceptor processes interlock, after which two buds from one process penetrated the other. Synaptic ribbons were observed in the cytoplasm of the photoreceptor cella, especially near the neuronal process. Macrophages were occasionally found to be contact with the outer segments of the photoreceptor cells in the pineal lumen.

• Microbiology and Biotechnology Letters
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• v.11 no.1
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• pp.75-79
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• 1983

To exploit a suitable vector and recipient strain for molecular cloning in Bacillus subtilis, oxytetracycline-resistant plasmic DNA has been prepared from Streptomyces rimosus by phenol-buffer extraction of lysozyme-lysed cells and introduced into B. subtilis KPM 60 [St $r^{R}$-mutant of RM 125 (leu A8, arg 15, hsm $M^{-10}$ , hsr $M^{-10}$ )] by transformation. Oxitetracycline-resistant plasmid was well transferred into B. subtilis KPM 60 with average frequency of 10$^{-4}$ per $\mu\textrm{g}$ of DNA. The highest frequency of plasmid transformation was obtained after 3 hours incubation of recipient cells in the growth medium and 30 to 60 minutes incubation in the competence medium, and then 20 minutes contact of DNA and host cells. Optimum pH for competence was 7.5, and optimum temperature for transformation was 2$0^{\circ}C$.>.

• The Korean Journal of Zoology
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• v.32 no.3
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• pp.258-263
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• 1989

Nuclear Transplantation between Rana pipiens and Rana dybowskii When diploid blastula nuclei of Rana pipiens are traraplanted into enucleated eggs of Rana dybowskii the resulting nucleocytoplasmic hybrids are lethal-those development were arrested around the stage of the dorsal lip formation For the improvement of developmental capacity, serial nuclear transplantation was carried out. Even though serial transplantation of 15 generations showed normal development in each generation until gastrula stage, there was no sign of fundamental improvement in development afterward. This results implied that up to gastrulation normal DNA replication and cell division can take place in foreign cytoplasm. Since chromosomal aberrations both in shape and number were usually observed, the nuclei must have been modifted while resided in the foreign cytoplasm. Those nuclei didn't participate in normal development and led the embryos to early death. Tissue graft experiment indicated that the abnormal behavior of this lethal nucleocytoplasmic hybrid is an inherent property which is not corrected by the contact with its own tissue.

• Applied Microscopy
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• v.34 no.4
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• pp.223-230
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• 2004

To identify the structural basis of mutations that affect synaptic transmission we have begun quantitative ultrastructural descriptions of synapses in cultured Drosophila embryonic neurons. In wild-type cultures, synapses are distinguished by the parallel arrangement of a thickened pre- and post synaptic membrane separated by a synaptic cleft. The presynaptic active zones and postsynaptic densities are defined by electron dense material close to the membrane. Presynaptic regions are also characterized by the presence of one or more electron dense regions, presynaptic densities, around which a variable number of small, clear core synaptic vesicles (mean $35.1{\pm}1.44$ nm in diameter) are clustered. Subsets of these vesicles are in direct contact with either the presynaptic density or the membrane and are considered morphologically docked. A small number of larger, dense core vesicles are also observed in most presynaptic profiles.

• Macromolecular Research
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• v.11 no.5
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• pp.368-374
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• 2003

Porous scaffolds composed of gelatin and polysaccharides such as hyaluronic acid and $\beta$-glucan were prepared by using the freeze-drying method after cross-linking with l-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). The scaffold had an inter-connected pore structure with the sufficient pore size for use as a support for the growth of fibroblasts. Results for the contact angle and cell attachment confirmed that high gelatin content in a mixture was suitable for cellular attachment and distribution in two- or three-dimensional fibroblast cultures. However, the addition of polysaccharides aroused the synergistic effects of morphologic and mechanical property of gelatin-based scaffolds. To prepare the artificial dermis for the wound dressing to mimic the normal human dermal skin, fibroblasts were isolated from a child's foreskin, and cultured in gelatin-based scaffolds. An in vivo study showed that the artificial dermis containing the fibroblasts enhanced the wound healing rate and re-epithelialization of a full-thickness skin defect rather than the acellular scaffold after one week.

• Transactions of the Korean hydrogen and new energy society
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• v.27 no.3
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• pp.276-282
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• 2016

The effects of conductive additives in a $La_{0.8}Sr_{0.2}MnO_3$ perovskite bifunctional electrode for oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) were investigated in an alkaline solution. Highly porous carbon black (CB) and Ni powder were added to the bifunctional electrodes as conductive additives. The surface morphologies of electrodes containing CB and Ni were observed by scanning electron microscopy (SEM). The current densities for both ORR and OER were changed by the addition of CB. The conductive additive changed physical properties of bifunctional electrodes such as the sheet conductance, gas permeability and contact angle. It was observed that the air permeability of electrode was most effective to enhance the currents for ORR and OER.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.20 no.6
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• pp.69-74
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• 2006

The effects of mechanical grinding(MG) treatment on the hydrogen storage of $Mg_2Ni$ alloy and $Mg_2Ni$ composite alloy($Mg_2Ni+graphite$) were investigated by pressure-composition-temperature(PCT) measurement, the micro-electrode technique of electrochemistry and etc, in which PCT was measured at high temperature(around $300[^{\circ}C]$) of gas phase and a carbon-filament micro-electrode for electrochemical evaluation was manipulated to make electrical contact with the particle in 1M KOH aqueous solution. It was found that the hydrogenation properties of $Mg_2Ni$ and graphite composite particle were greatly improved by the mechanical grinding treatment by which the $Mg_2Ni$ and graphite composite alloys could be changed into microstructure and nano-level particles. namely; the hydrogen dissociation pressure of PCT measurement was decreased from 0.55[MPa] to 0.42[MPa] and hydrogenation peaks by micro-electrode were also observed more clearly on the same sample.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.1 no.1
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• pp.23-26
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• 2000

In spot welded joint. Electronic Speckle Pattern Interferometry(ESPI) method using the Model 95 Ar laser a video system and an image processor was applied to measure the stress Unlike traditional strain gauges or Moire method, ESPI method has no special surface preparation or attachments and can be measured in-plane displacement with non-contact and real time. In this experiment, specimens are loaded in parallel with a load cell. The specimens are made of the cold rolled steel sheet with 1mm thickness, are attached strain. gauges. This study Provides an example of how ESPI has been used to measure stress and strain inspecimen. The results measured by ESPI are compared with the data which was measured by strain gauge method under tensile testing.

• Korean Chemical Engineering Research
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• v.57 no.2
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• pp.210-218
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• 2019

Batch experiment studies were carried out on the adsorption of the major tar compound, 2-picoline, derived from the plant cell cultures of Taxus chinensis, using Sylopute while varying parameters such as initial 2-picoline concentration, contact time and adsorption temperature. The experimental data were fitted to the Langmuir, Freundlich, Temkin and Dubinin-Radushkevich isotherm models. Comparison of results revealed that the Langmuir isotherm model could account for the adsorption isotherm data with the highest accuracy among the four isotherm models considered. From the analysis of adsorption isotherms, it was found that adsorption capacity decreased with increasing temperature and the adsorption of 2-picoline onto Sylopute was favorable. The kinetic data were well described by the pseudo-second-order kinetic model, while intraparticle diffusion and boundary layer diffusion did not play a dominated role in 2-picoline adsorption according to the intraparticle diffusion model. Thermodynamic parameters revealed the exothermic, irreversible and non-spontaneous nature of adsorption. The isosteric heat of adsorption decreased as surface loading ($q_e$) increased, indicating a heterogeneous surface.