• Journal of Microbiology and Biotechnology
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• v.33 no.10
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• pp.1257-1267
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• 2023

Although rational genetic engineering is nowadays the favored method for microbial strain improvement, building up mutant libraries based on directed evolution for improvement is still in many cases the better option. In this regard, the demand for precise and efficient screening methods for mutants with high performance has stimulated the development of biosensor-based high-throughput screening strategies. Genetically encoded biosensors provide powerful tools to couple the desired phenotype to a detectable signal, such as fluorescence and growth rate. Herein, we review recent advances in engineering several classes of biosensors and their applications in directed evolution. Furthermore, we compare and discuss the screening advantages and limitations of two-component biosensors, transcription-factor-based biosensors, and RNA-based biosensors. Engineering these biosensors has focused mainly on modifying the expression level or structure of the biosensor components to optimize the dynamic range, specificity, and detection range. Finally, the applications of biosensors in the evolution of proteins, metabolic pathways, and genome-scale metabolic networks are described. This review provides potential guidance in the design of biosensors and their applications in improving the bioproduction of microbial cell factories through directed evolution.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.32 no.10
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• pp.844-849
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• 2008

We present a novel cell deformability monitoring chip based on the digitally measured cell lysis rate which is dependent on the areal strain of the cell membrane. This method offers simple cell deformability monitoring by automated high-throughput testing system. We suggest the filter design considering the areal strain imposed on the cell membrane passing through the filter array having gradually increased orifice length. In the experiment using erythrocytes, we characterized the cell deformability in terms of average fracture areal strain which was $0.24{\pm}0.014\;and\;0.21{\pm}0.002$ for normal and chemically treated erythrocytes, respectively. We also verified that the areal strain of 0.15 effectively discriminates the deformability difference of normal and chemically treated erythrocytes, which can be applied to the clinical situation. We compared the lysis rates and their difference for the samples from different donors and found that the present chips can be commonly used without any calibration process. The experimental results demonstrate the simple structure and high performance of the present cell deformability monitoring chips, applicable to simple and cost-effective cell aging process monitoring.

• JSTS:Journal of Semiconductor Technology and Science
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• v.15 no.3
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• pp.427-435
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• 2015

This paper presents a high-throughput low-complexity decoder architecture and design technique to implement successive-cancellation (SC) polar decoding. A novel merged processing element with a one's complement scheme, a main frame with optimal internal word length, and optimized feedback part architecture are proposed. Generally, a polar decoder uses a two's complement scheme in merged processing elements, in which a conversion between two's complement and sign-magnitude requires an adder. However, the novel merged processing elements do not require an adder. Moreover, in order to reduce hardware complexity, optimized main frame and feedback part approaches are also presented. A (1024, 512) SC polar decoder was designed and implemented using 40-nm CMOS standard cell technology. Synthesis results show that the proposed SC polar decoder can lead to a 13% reduction in hardware complexity and a higher clock speed compared to conventional decoders.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.16 no.8
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• pp.2648-2665
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• 2022

Device-to-device (D2D) multicast has become a promising technology to provide specific services within a small geographical region with a high data rate, low delay and low energy consumption. However, D2D multicast communications are allowed to reuse the same channels with cellular uplinks and result in mutual interference in a cell. In this paper, an intelligent channel assignment algorithm is designed in D2D underlaid cellular networks with the target of maximizing network throughput. We first model the channel assignment problem to be a throughput maximizing problem which is NP-hard. To solve the problem in a feasible way, a novel channel assignment algorithm is proposed. The key idea is to find the appropriate cellular communications and D2D multicast groups to share a channel without causing critical interference, i.e., finding a channel for a D2D multicast group which generates the least interference to network based on current channel assignment status. In order to show the efficacy and effectiveness of our proposed algorithm, a novel search algorithm is proposed to find the near-optimal solution as the baseline for comparisons. Simulation results show that the proposed algorithm improves the network throughput.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38A no.3
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• pp.268-276
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• 2013

In this paper, we introduce the technologies to handle the interference in the heterogeneous network and evaluate the performance of enhanced Inter-Cell Interference Coordination (enhanced ICIC, eICIC) techniques that are being introduced in 3GPP Release 10. In the time-domain eICIC scheme, time-domain resources are scheduled to avoid the interference by using Almost Blank Subframe (ABS) and Cell Range Expansion (CRE). To mitigate the cross-tier interference between macro and femtocell, it is important to efficiently combine the ABS and CRE in heterogeneous network. Since it is hard to evaluate the total throughput of heterogeneous network numerically, we evaluate the total throughput by using system level simulation (SLS). As a result of evaluation, the throughputs of many different cases of combination of ABS and CRE are compared.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.12A
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• pp.1191-1199
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• 2008

We propose the Staggered-zone Resource Allocation (SRA) in order to relax throughput decrease problems by the co-channel interference of the cell boundary users at the cellular OFDMA system using frequency reuse factor K=1 and analyze the throughput improvement. The proposed algorithm allocates the resources to the users in compliance with resource allocation rule which is planned in order to minimize co-channel interference between cells without any additional information. The resource allocation method in the SRA lines up the users in pathloss order as descending series, and then allocates from pre-determined resource allocation region where decides differently in each cell. This algorithm prevents the co-channel interferences of the cell boundary user to be caused by using same resource simultaneously and equalizes interference to the users in the cell.

• Journal of Communications and Networks
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• v.18 no.4
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• pp.639-648
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• 2016

Next-generation (4G) systems are designed to support universal frequency reuse (UFR) to achieve best use of valuable spectra. However, it leads to undesirable interference level near cell borders. To control this, 4G systems adopt techniques, such as network multiple-input multiple-output (MIMO) and inter-cell interference coordination (ICIC), to improve cell-edge throughput. Network MIMO aims at mitigating inter-cell interference towards cell-edge users (CEUs) through multi-cell cooperation, where each collaborative base station serves both cell-center users (CCUs) and CEUs, including other cells' CEUs, under a power constraint. The present ICIC strategies cannot be directly applied to network MIMO because they were designed in absence of multi-cell coordination. In the presence of network MIMO, this paper investigates antenna orientations in ICIC and the method of power management. Results show that a proper antenna orientation can improve the cell-edge capacity and meantime lower the interference to CCUs. Capacity inconsistency between CCUs and CEUs is detrimental to mobile communications. Simulation results show that the proposed power management for ICIC in network MIMO systems can achieve a uniform data rate regardless users' position.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.3
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• pp.178-184
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• 2002

High-throughput screening has become a popular method used to identify new “leads”for potentially therapeutic compounds. Further screening of these lead compounds is typically done with secondary assays which may utilize living, functioning cells as screening tools. A problem (or benefit) with these cell-based assays is that living cells are very sensitive to their environment. We have been interested in the process of stem cell migration and how it relates to the cellular therapy of bone marrow transplantation. In this study we describe a secondary, cell-based assay for screening the effects of various in-vitro conditions on Immature Hematopoietic Cell (IHC) migration. Our results have revealed many subtle factors, such as the cell's adhesive characteristics, or the effect of a culture's growth phase, that need to be accounted for in a screening protocol. Finally, we show that exponentially glowing KG1a cells (a human IHC cell line) were 10 times more motile than those in the lag or stationary phases. These data strongly suggest that KG1a cells secrete a chemokinetic factor during the exponential growth phase of a culture.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2003.05a
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• pp.390-393
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• 2003

In this paper, we study on an implementation of cell scheduler which arbitrates the ATM exchange efficiently and swiftly. The designed ATM cell scheduler of this paper is based on iSLIP scheduling algorithm. It is aimed at the high-speed implementation. The implemented cell scheduler approximately provides 100% throughput for cell scheduling. We present a basic structure for cell scheduler and describe by using the HDL and perform behavior level and timing simulation. The cell scheduler of this paper is designed to support 8-port switch fabric and can expand in 32-port switch fabric. The cell scheduler for supporting the 8-port switch fabric is designed in 2-stage pipelines for the grant and accept stages respectively.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.2
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• pp.86-92
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• 2004

In this paper miniaturized disposable micro/nanofluidic components applicable to bio chip, chemical analyzer and biomedical monitoring system, such as blood analysis, micro dosing system and cell experiment, etc are reported. This system includes various microfluidic components including a micropump, micromixer, DNA purification chip and single-cell assay chip. For low voltage and low power operation, a surface tension-driven micropump is presented, as well as a micromixer, which was implemented using MEMS technology, for efficient liquid mixing is also introduced. As bio-reactors, DNA purification and single-cell assay devices, for the extraction of pure DNA from liquid mixture or blood and for cellular engineering or high-throughput screening, respectively, are presented.