• Journal of Microbiology and Biotechnology
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• 제33권10호
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• pp.1257-1267
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• 2023

Although rational genetic engineering is nowadays the favored method for microbial strain improvement, building up mutant libraries based on directed evolution for improvement is still in many cases the better option. In this regard, the demand for precise and efficient screening methods for mutants with high performance has stimulated the development of biosensor-based high-throughput screening strategies. Genetically encoded biosensors provide powerful tools to couple the desired phenotype to a detectable signal, such as fluorescence and growth rate. Herein, we review recent advances in engineering several classes of biosensors and their applications in directed evolution. Furthermore, we compare and discuss the screening advantages and limitations of two-component biosensors, transcription-factor-based biosensors, and RNA-based biosensors. Engineering these biosensors has focused mainly on modifying the expression level or structure of the biosensor components to optimize the dynamic range, specificity, and detection range. Finally, the applications of biosensors in the evolution of proteins, metabolic pathways, and genome-scale metabolic networks are described. This review provides potential guidance in the design of biosensors and their applications in improving the bioproduction of microbial cell factories through directed evolution.

• 대한기계학회논문집A
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• 제32권10호
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• pp.844-849
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• 2008

We present a novel cell deformability monitoring chip based on the digitally measured cell lysis rate which is dependent on the areal strain of the cell membrane. This method offers simple cell deformability monitoring by automated high-throughput testing system. We suggest the filter design considering the areal strain imposed on the cell membrane passing through the filter array having gradually increased orifice length. In the experiment using erythrocytes, we characterized the cell deformability in terms of average fracture areal strain which was $0.24{\pm}0.014\;and\;0.21{\pm}0.002$ for normal and chemically treated erythrocytes, respectively. We also verified that the areal strain of 0.15 effectively discriminates the deformability difference of normal and chemically treated erythrocytes, which can be applied to the clinical situation. We compared the lysis rates and their difference for the samples from different donors and found that the present chips can be commonly used without any calibration process. The experimental results demonstrate the simple structure and high performance of the present cell deformability monitoring chips, applicable to simple and cost-effective cell aging process monitoring.

• 한국통신학회논문지
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• 제38A권3호
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• pp.268-276
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• 2013

본 연구에서는 Heterogeneous Network에서의 간섭제어에 관한 기술 동향을 살펴보고, 간섭 제어 기술 중 3GPP release 10에서의 주요 기술 enhanced Inter-Cell Interference Coordination (enhanced ICIC, eICIC)을 택하여 성능을 분석해 보았다. eICIC기술 중 시간영역에서의 간섭 제어인 Almost Blank Subframe (ABS)와 Cell Range Expansion (CRE) 기술에 의한 성능을 분석하였다. Heterogeneous Network에서 macrocell과 femtocell 간의 간섭 제어를 적절히 하기 위해서는 ABS기술과 CRE 기술 간의 조합이 중요할 것으로 보인다. Heterogeneous Network의 전체 성능 평가는 정량적으로는 분석되기 어려워 system level simulation에 의해서 평가하였다. 본 연구에서는 SLS 방식으로 다양한 환경에 대한 성능을 분석함으로써 Heterogeneous Network 운영에 있어 각각의 요소가 미치는 영향을 파악할 수 있도록 하였으며, Heterogeneous Network에서의 ABS와 CRE의 결합에 의한 효과를 분석하였다.

• 한국통신학회논문지
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• 제33권12A호
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• pp.1191-1199
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• 2008

주파수 재사용도 K = 1을 사용하는 셀룰러 OFDMA 시스템에서 셀 경계 사용자의 동일 채널 간섭에 의한 수율 저하 문제를 완화하기 위한 대역 분산적 자원 할당 알고리즘을 제안하고, 이에 따른 성능 향상 효과를 분석한다. 본 논문에서 고려한 대역 분산적 자원 할당 알고리즘은 셀 내의 사용자를 경로 감쇄 순서로 내림차순으로 정렬한 후에 각 셀마다 다르게 미리 정해져 있는 자원 할당 영역부터 자원을 할당한다. 셀마다 다른 시간과 공간적인 영역에서 자원 할당을 시작하도록 되어 있으므로 셀 경계의 사용자부터 셀 중앙의 사용자들까지 셀 내 특정위치의 사용자를 특정 시간대에 배치하여 셀 경계 사용자들이 동시에 같은 자원을 사용하여 간섭이 매우 커지는 경우를 미리 방지하고 셀 내의 사용자들에게 간섭이 평준화되어 고르게 영향을 받도록 한다. 이러한 대역 분산적 자원 할당 방식을 사용하여 셀 경계 사용자의 전송률과 outage 성능이 향상되었음을 확인하였다.

• JSTS:Journal of Semiconductor Technology and Science
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• 제15권3호
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• pp.427-435
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• 2015

This paper presents a high-throughput low-complexity decoder architecture and design technique to implement successive-cancellation (SC) polar decoding. A novel merged processing element with a one's complement scheme, a main frame with optimal internal word length, and optimized feedback part architecture are proposed. Generally, a polar decoder uses a two's complement scheme in merged processing elements, in which a conversion between two's complement and sign-magnitude requires an adder. However, the novel merged processing elements do not require an adder. Moreover, in order to reduce hardware complexity, optimized main frame and feedback part approaches are also presented. A (1024, 512) SC polar decoder was designed and implemented using 40-nm CMOS standard cell technology. Synthesis results show that the proposed SC polar decoder can lead to a 13% reduction in hardware complexity and a higher clock speed compared to conventional decoders.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제16권8호
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• pp.2648-2665
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• 2022

Device-to-device (D2D) multicast has become a promising technology to provide specific services within a small geographical region with a high data rate, low delay and low energy consumption. However, D2D multicast communications are allowed to reuse the same channels with cellular uplinks and result in mutual interference in a cell. In this paper, an intelligent channel assignment algorithm is designed in D2D underlaid cellular networks with the target of maximizing network throughput. We first model the channel assignment problem to be a throughput maximizing problem which is NP-hard. To solve the problem in a feasible way, a novel channel assignment algorithm is proposed. The key idea is to find the appropriate cellular communications and D2D multicast groups to share a channel without causing critical interference, i.e., finding a channel for a D2D multicast group which generates the least interference to network based on current channel assignment status. In order to show the efficacy and effectiveness of our proposed algorithm, a novel search algorithm is proposed to find the near-optimal solution as the baseline for comparisons. Simulation results show that the proposed algorithm improves the network throughput.

• Journal of Communications and Networks
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• 제18권4호
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• pp.639-648
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• 2016

Next-generation (4G) systems are designed to support universal frequency reuse (UFR) to achieve best use of valuable spectra. However, it leads to undesirable interference level near cell borders. To control this, 4G systems adopt techniques, such as network multiple-input multiple-output (MIMO) and inter-cell interference coordination (ICIC), to improve cell-edge throughput. Network MIMO aims at mitigating inter-cell interference towards cell-edge users (CEUs) through multi-cell cooperation, where each collaborative base station serves both cell-center users (CCUs) and CEUs, including other cells' CEUs, under a power constraint. The present ICIC strategies cannot be directly applied to network MIMO because they were designed in absence of multi-cell coordination. In the presence of network MIMO, this paper investigates antenna orientations in ICIC and the method of power management. Results show that a proper antenna orientation can improve the cell-edge capacity and meantime lower the interference to CCUs. Capacity inconsistency between CCUs and CEUs is detrimental to mobile communications. Simulation results show that the proposed power management for ICIC in network MIMO systems can achieve a uniform data rate regardless users' position.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권3호
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• pp.178-184
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• 2002

High-throughput screening has become a popular method used to identify new “leads”for potentially therapeutic compounds. Further screening of these lead compounds is typically done with secondary assays which may utilize living, functioning cells as screening tools. A problem (or benefit) with these cell-based assays is that living cells are very sensitive to their environment. We have been interested in the process of stem cell migration and how it relates to the cellular therapy of bone marrow transplantation. In this study we describe a secondary, cell-based assay for screening the effects of various in-vitro conditions on Immature Hematopoietic Cell (IHC) migration. Our results have revealed many subtle factors, such as the cell's adhesive characteristics, or the effect of a culture's growth phase, that need to be accounted for in a screening protocol. Finally, we show that exponentially glowing KG1a cells (a human IHC cell line) were 10 times more motile than those in the lag or stationary phases. These data strongly suggest that KG1a cells secrete a chemokinetic factor during the exponential growth phase of a culture.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2003년도 춘계종합학술대회
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• pp.390-393
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• 2003

본 논문에서는 ATM 교환기의 스위치 패브릭을 효과적이고, 빠르게 중재할 수 있는 Cell 스케줄링 알고리즘의 구현에 대해 연구한다. 본 논문에서 설계하는 ATM 셀 스케줄러는 iSLIP 알고리즘을 기본으로 하고 있으며, 이의 고속 구현에 대해 연구한다. 구현되는 셀 스케줄러는 random uniform 트래픽에 대해 100%에 수렴하는 스케줄링 성능을 제공하고 있다. 따라서 본 연구에서는 기본적인 스케줄러의 구조를 제안하고, 이를 HDL로 모델하여 동작 수준 및 타이밍 시뮬레이션을 완료하였다. 그리고 본 논문에서 설계된 셀 스케줄러는 8 포트를 지원하도록 설계하였으며, 이를 기반으로 하여 32 포트로 확장할 수 있다. 8 포트를 지원하는 스케줄러는 grant 및 accept 스테이지를 각각 2단 파이프라인 방식으로 설계하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권2호
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• pp.86-92
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• 2004

In this paper miniaturized disposable micro/nanofluidic components applicable to bio chip, chemical analyzer and biomedical monitoring system, such as blood analysis, micro dosing system and cell experiment, etc are reported. This system includes various microfluidic components including a micropump, micromixer, DNA purification chip and single-cell assay chip. For low voltage and low power operation, a surface tension-driven micropump is presented, as well as a micromixer, which was implemented using MEMS technology, for efficient liquid mixing is also introduced. As bio-reactors, DNA purification and single-cell assay devices, for the extraction of pure DNA from liquid mixture or blood and for cellular engineering or high-throughput screening, respectively, are presented.