• 제목/요약/키워드: Cell test

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연료전지자동차의 고압수소저장시스템 신뢰성 평가(II) (The Evaluation of Reliability for the High pressure hydrogen Storage System of Fuel Cell Vehicle(II))

  • 최영민;김상현;김형기;장규진;안병기;임태원
    • 한국신재생에너지학회:학술대회논문집
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    • 한국신재생에너지학회 2008년도 춘계학술대회 논문집
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    • pp.37-40
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    • 2008
  • We have concentrated on the performance improvement of each part for durability, safety and cost of high pressure storage system for fuel cell vehicle so far. But for the mass production of fuel cell vehicle, it is necessary to evaluate durability and safety in system module. We built the standard to evaluate and collision safety of high pressure storage system for fuel cell vehicle, and could verify reliability of high pressure storage system.

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연료전지자동차의 고압수소저장시스템 신뢰성 평가 (The Evaluation of Reliability for the High pressure hydrogen Storage System of Fuel Cell Vehicle)

  • 장규진;최영민;안병기;임태원
    • 한국신재생에너지학회:학술대회논문집
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    • 한국신재생에너지학회 2007년도 추계학술대회 논문집
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    • pp.71-74
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    • 2007
  • We have concentrated on the performance improvement of each part for durability, safety and cost of high pressure storage system for fuel cell vehicle so far. But for the mass production of fuel cell vehicle, it is necessary to evaluate durability and safety in system module. We built the standard to evaluate vibration and collision safety of high pressure storage system for fuel cell vehicle, and could verify reliability of high pressure storage system.

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신경회로망을 이용한 지능형 로봇 제어 시스템 설계 (Design of an Intelligent Robot Control System Using Neural Network)

  • 정동연
    • 한국공작기계학회:학술대회논문집
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    • 한국공작기계학회 2000년도 춘계학술대회논문집 - 한국공작기계학회
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    • pp.182-187
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    • 2000
  • In this paper, we have proposed a new approach to the design of robot vision system to develop the technology for the automatic test and assembling of precision mechanical and electronic parts for the factory automation. In order to perform real time implementation of the automatic assembling tasks in the complex processes, we have developed an intelligent control algorithm based-on neural networks control theory to enhance the precise motion control. Implementing of the automatic test tasks has been performed by the real-time vision algorithm based-on TMS320C31 DSPs. It distinguishes correctly the difference between the acceptable and unacceptable defective item through pattern recognition of parts by the developed vision algorithm. Finally, the performance of proposed robot vision system has been illustrated by experiment for the similar model of fifth cell among the twelve cell for similar model of fifth cell among the twelve cell for automatic test and assemblig in S company.

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GLP-Application to Cell Culture-Based Toxicity Tests

  • Koh, Woo-Suk
    • 한국독성학회:학술대회논문집
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    • 한국독성학회 2006년도 추계학술대회
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    • pp.95-101
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    • 2006
  • Compare to the toxicity tests using experimental animals, the GLP application and compliance in toxicity studies using cell culture systems may be less straightforward elucidated in the two documents published by the OECD Working Croup on GLP 'The Application of the GLP Principles to Short Term Studies (1999)' and 'The Application of the Principles of GLP to in vitro Studies (2004)' The object of this presentation is to show how to interpret the GLP principles and to apply with actual performances in a well known toxicity test using cell culture, chromosome aberration study. The presentation will cover test substance, test system (cell line), study environment management, documentation, quality assurance, and study protocol and report.

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회전익항공기 연료셀 충돌충격시험 Full-Scale 수치모사 (Numerical Simulation of Full-Scale Crash Impact Test for Fuel Cell of Rotorcraft)

  • 김현기;김성찬;김성준;김수연
    • 한국전산구조공학회논문집
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    • 제26권5호
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    • pp.343-349
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    • 2013
  • 항공기 연료셀은 추락 상황에서 승무원의 생존성과 직결되는 중요 구성품으로 회전익 항공기에 적용되고 있는 내충격성 연료셀은 추락시 승무원의 생존성 향상에 큰 역할을 하고 있다. 미육군은 항공기가 처할수 있는 다양한 상황에서 연료셀이 제 기능을 발휘할 수 있도록 1960년대 초부터 MIL-DTL-27422 이라는 연료셀 개발규격을 제정하여 현재까지 적용해 오고 있다. 해당 개발규격에 규정된 시험 중에서 충돌충격시험은 연료셀의 내충격 성능을 검증하는 시험으로써, 해당 시험을 통과하는 연료셀은 생존가능 충돌환경에서 화재가 발생하지 않아 승무원의 생존성이 대폭 향상될 수 있음을 의미한다. 그러나 충돌충격시험은 작용하는 하중 수준이 너무 높기 때문에 실패 위험성이 가장 큰 시험이기도 하다. 연료셀이 해당 시험을 통과하지 못하는 경우에는 재시험을 위한 비용과 준비기간이 상당히 소요되어 항공기 개발일정에 심각한 지장을 초래할 가능성도 높다. 따라서, 연료셀 설계 초기부터 내충격성능 만족여부에 대한 예측을 위해 충돌충격시험의 수치해석을 통한 실물시험에서의 실패 가능성을 최소화해야 한다는 필요성이 제기되어 왔다. 본 연구에서는 충돌모사 프로그램인 LS-DYNA에서 지원하는 유체-구조 연성해석 방법인 SPH 방법을 사용하여 연료셀 충돌충격시험 수치 모사를 수행하였다. 수치해석 조건으로 MIL-DTL-27422에서 요구하는 시험조건을 고려하였고, 실물 연료셀의 시편시험을 통해 확보한 물성데이타를 해석에 반영하였다. 그 결과로 연료셀 자체의 응력수준을 평가하고 취약부위에 대한 고찰을 수행하였다.

근관 충전용 시멘트의 치주인대 섬유아세포 독성에 관한 연구 (THE CYTOTOXIC EFFECTS OF ROOT CANAL SEALERS ON FIBROBLASTS IN HUMAN PERIODONTAL LIGAMENT)

  • 이광희;최호영;민병순;박상진;최기운
    • Restorative Dentistry and Endodontics
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    • 제18권1호
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    • pp.156-172
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    • 1993
  • The purpose of this study was to evaluate for the cytotoxicity of root canal sealers - Tubliseal, N2, AH26, and Sealapex - on fibroblasts cultivated from human periodontal ligament. Succinate dehydrogenase activity test and $^{51}Cr$ release test were performed to evaluate the shortterm cytotoxicity. According to l,3 and 6 days vital cell count and $^{14}C$-leucine incorporation rate to fibroblasts were evaluated. The results of this study were as follows: 1. In the test of SDH activity by millipore filter method, Sealapex showed mild cytotoxicity but Tubliseal, N2 and AH26 showed severe cytotoxicity. 2. In $^{51}Cr$ release test, Tubliseal was the most cytotoxic sealer tested, and rank ordered the relative cytotoxicity of the other sealers tested as follows: AH26, N2, Sealapex. 3. In the test of viable cell count, cytotoxicity in Tubliseal was continued because vital cell number reduced with time. Because vital cell was not showed in N2 and AH26 at 1 day, it was recognized that N2 and AH26 exhibited severe cytotoxicity. In Sealapex, vital cell number increased remarkably with time, so it showed that cytotoxicity decreased with time. 4. In $^{14}C$-leucine incorporation rate test, protein sythesis was not produced in Tubliseal, N2 and AH26 after 3 days, it showed that cytotoxicity in Tubliseal, N2 and AH26 was severe. Although protein synthesis in Sealapex decreased with time, it continued after 6 days. Therefore Sealapex has been exhibited mild cytotoxicity.

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오스터버그 셀$(O-cell^{\circledR})$을 이용한 말뚝 정재하시험의 국내 적용 (Osterberg Cell $(O-cell^{\circledR})$ method of Static Pile Load Testing in Korea)

  • Molnit, Thomas
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2006년도 춘계 학술발표회 논문집
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    • pp.520-529
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    • 2006
  • 그 동안 몇 차례 오스터버그 셀$(O-cell^{\circledR})$을 이용한 양방향 말뚝 재하시험기법이 국내에 성공적으로 소개/적용된 바 있다. 이 방법은 상대적으로 새롭고 독특한 방법으로서 시험비용과 기존 재래적인 시험방법이 적용상 제한을 받는 경우나 대규모 시험에서 발생할 수 있는 문제점들을 극복할 수 있다. 국내에서는 설계목적을 위한 시험말뚝 뿐 아니라 설계하중이 큰 실제말뚝에 대한 시험이 증가하는 추세에 있다. 이 논문에서 소개하는 양방향 재하시험 방법을 적용하면 기존의 소규모 재하시험 방법에서 발생될 수 있는 주면 마찰력과 선단지지력의 분리측정이나 각각의 지지력에 대한 극한상태를 확인하지 못하는 한계를 극복할 수 있고 시험하중이 270MN을 초과하는 경우까지 적용할 수 있는 장점을 갖고 있다. 본 논문에서는 오스터버그 셀을 이용한 정적 재하시험 방법에 대하여 말뚝종류별로 상세히 기술하였으며 이 시험방법의 장점과 국내현장에 적용되었던 사례를 소개하였다.

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A drug screening system: Preparation of cell islets on the hydrophilic surface

  • 권혁성;배근원;정경희;김익환
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2002년도 생물공학의 동향 (X)
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    • pp.581-584
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    • 2002
  • Individual surface(hydrophilic/hydrophobic) were prepared and mammalian cells were cultured on the hydrophilic region. For drug test, cancer and normal cells were treated with Taxol, as an example. Our system was compared with MTT assay. CHO cells were resistant to Taxol up to 100 nM in both Methods. However, A549 cells was sensitive at 100 nM Taxol in the 2 day-treatment. Cervical carcinoma cell, HeLa, was very sensitive to Taxol. In our system, the cells were not shown from above 20 nM Taxol treatment. Our system was competitive to MTT assay in animal cells for drug test.

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전자기장 프로브 교정용 비대칭형 TEM cell의 설계 및 성능 평가 (Design and performance of asymmetric TEM cell for calibration field probes)

  • 윤재훈;이혁재;황호정
    • 한국통신학회논문지
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    • 제25권1B호
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    • pp.48-55
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    • 2000
  • 본 논문에서는 전기장 프로브 교정용 비대칭형 TEM cell의 설계과정과 제작결과에 대해 나타내었다. 2cm$\times$2cm인 시험 공간(test space)에서 전자기장의 균일도가 $\pm$2 ㏈내를 유지하면서, 제1공진 주파수는 1.5GHz 이하에서 출현하도록 하고 제2공진 주파수는 약 GHz에서 출현할 수 있도록 제작하였다. 제작 결과 계산된 결과와 실험된 데이터가 일치함을 볼 수가 있었다.

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Mast Cell Concentrations in Peripheral and Central Giant Cell Granulomas: Is there any Angiogenetic Role?

  • Farhadi, Sareh;Shahsavari, Fatemeh;Taleghani, Ferial;Komasi, Elaheh
    • Asian Pacific Journal of Cancer Prevention
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    • 제17권2호
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    • pp.673-676
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    • 2016
  • Background: In the maxillofacial region, giant cell granulomas occur in 2 clinical forms, central and peripheral. Despite histopathological similarity between these 2 forms totally different clinical behaviors have been reported. The present study was undertaken to compare mast cell and vascular concentrations in these pathologic lesions. Materials and Methods: In this cross-sectional descriptive study, 20 pathological samples of central giant cell granuloma (CGCG) and 20 samples of peripheral giant cell granuloma (PGCG) were selected and examined through toluidine blue staining for mast cell assessment and immunohistochemical staining by VEGEF antibody for comparing the number of mast cells. T-test, chi-squared test and backward multivariate linear regression were used for statistical analysis using SPSS 20. Statistical significance was set at P<0.05. Results: This study showed significantly greater VEGF expression and mast cell concentrations in CGCG compared to PGCG cases. Also there was a significant correlation between VEGF expression and the concentration of mast cells. No relation was found between age, sex and site of the lesion and concentration of mast cells or VEGF expression. Conclusions: It is feasible that higher concentrations of mast cells in CGCG versus PGCG samples might lead to more aggressive clinical behavior via vascular proliferation and angiogenesis. However, other biologic mechanisms should be considered in this situation.