• 제목/요약/키워드: Cell surface properties

검색결과 766건 처리시간 0.026초

시간영역 변위근사 유한차분법의 자유면 경계조건 (Free-surface Boundary Condition in Time-domain Elastic Wave Modeling Using Displacement-based Finite-difference Method)

  • 민동주;유해수
    • 지구물리와물리탐사
    • /
    • 제6권2호
    • /
    • pp.77-86
    • /
    • 2003
  • 자유면 경계조건을 정착하게 묘사할 수 있는 변위근사 유한차분법을 이용하는 시간영역 탄성파 모델링법을 고안하였다. 기존의 변위근사 유한차분법의 경우 변위와 매질의 물성을 격자점에 정의하는 격자군(격자점 기반의 격자군)을 이용하였으나, 이 연구에서 제시하는 새로운 유한차분법에서는 변위는 격자점에 정의하지만 매질의 물성을 격자점으로 둘러싸인 면에 정의하는 격자군(셀 기반의 격자군)을 이용한다. 매질의 물성을 셀에 정의할 경우 자유면에서 응력이 사라진다는 자유면 경계조건을 추가로 적용할 필요가 없으며 매질의 물성 변화만으로 자유면 경계조건을 표현할 수 있다. 수치예를 통한 정확도 분석 결과 셀 기반의 격자군을 이용할 경우 계산된 수치석인 해가 해석적인 해에 매우 근사함을 알 수 있었다.

Novel Properties for Endoglucanase Acquired by Cell-Surface Display Technique

  • Shi, Baosheng;Ke, Xiaojing;Yu, Hongwei;Xie, Jing;Jia, Yingmin;Guo, Runfang
    • Journal of Microbiology and Biotechnology
    • /
    • 제25권11호
    • /
    • pp.1856-1862
    • /
    • 2015
  • In order to improve the stability of endoglucanase under thermal and acidic conditions, the endoglucanase gene was fused to the N-terminus of the Saccharomyces cerevisiae pir gene, encoding the cell wall protein PIR. The fusion gene was transformed into Pichia pastoris GS115 for expression. A resulting strain with high expression and high activity was identified by examining resistance to Geneticin 418, Congo red staining, and quantitative analysis of enzyme activity. SDS-PAGE analysis revealed that the endoglucanase was successfully displayed on the yeast cell surface. The displayed endoglucanase (DEG) showed maximum activity towards sodium carboxyl methyl cellulose at approximately 275 IU/g cell dry weight. DEG exhibited greater than 60% residual activity in the pH range 2.5-8.5, higher than free endoglucanase (FEG), which had 40% residual activity at the same pH range. The highest tolerated temperature for DEG was 70℃, much higher than that of FEG, which was approximately 50℃. Moreover, DEG showed 91.1% activity at 65℃ for 120 min, while FEG only kept 77.8% residual activity over the same period. The half-life of DEG was 270 min at 65℃, compared with only 150 min for FEG. DEG could be used repeatedly at least three times. These results suggest that the DEG has broad applications as a yeast whole-cell biocatalyst, due to its novel properties of high catalytic efficiency, acid-thermal stabilities, and reusability.

ULTRAVIOLET MICROSCOPIC STUDY ON LIGNIN DISTRIBUTION IN THE FIBER CELL WALL OF BCTMP

  • Seung-Lak YooN;Yasuo KOJIMA;Lee, Seon-Ho
    • 한국펄프종이공학회:학술대회논문집
    • /
    • 한국펄프종이공학회 1999년도 Pre-symposium of the 10th ISWPC Recent Advances in Paper Science and Technology
    • /
    • pp.375-380
    • /
    • 1999
  • In order to improve the optical properties of high yield pulp, bleached chemi-thermo-mechanical pulp (BCTMP) was produced from CTMP of Betula maximowicziana Regel by two staged ozone-hydrogen peroxide bleaching. This pulp was used for the evaluation of the improvement of optical properties, chemical characteristics of lignin in fiber, and the relationship between lignin and optical properties in fiber cell wall. By hydrogen peroxide treatment, the brightness was improved, but the post color number (PC No.) was not. There was little improvement on optical properties by ozone treatment, but his could be solved by using two staged ozone-hydrogen peroxide bleaching. The hydrogen peroxide treatment did not make nay change on chemical characteristics of lignin in cell wall, but by ozone treatment, it was found that the non-aromatic conjugated structure was existed in the surface of cell wall, but this could be removed by hydrogen peroxide treatment in two staged ozone-hydrogen peroxide treatment. Therefore, the optical properties was significantly improved due to the removal of non-aromatic conjugated structure.

SMD 타입 태양전지 어레이를 이용한 white GaN LED용 전원 공급 장치 (Power Supply for White GaN LED by Using SMD Type Solar Cell Array)

  • 김성일;이윤표
    • 신재생에너지
    • /
    • 제5권4호
    • /
    • pp.34-37
    • /
    • 2009
  • Using six SMD(surface mount device) type AlGaAs/GaAs single junction solar cells connected in series, a power source was fabricated for a white GaN LED. The electrical properties of the power source was measured and analyzed under one sun (100mW/$cm^2$) and various indoor light (300 - 900 lux) conditions. Under 600 lux indoor light condition, output power was 17.06 ${\mu}W$ and it was 30.75 ${\mu}W$ under 900 lux indoor light condition. Using the fabricated solar cell power supply, we have turned on the white GaN LED. It was worked well under 15 ${\mu}W$(at 480 lux) power supplied from solar cell array. This kind of solar cell power supply can be used as a power source for ubiquitous sensor network (USN).

  • PDF

Investigation of the Corrosive Chemical Interaction on Antireflective Layers of Solar Cell Multilayers

  • 최성현;김선미;진숙영;박정영
    • 한국진공학회:학술대회논문집
    • /
    • 한국진공학회 2011년도 제41회 하계 정기 학술대회 초록집
    • /
    • pp.187-187
    • /
    • 2011
  • Nowadays, the issue of solar cell durability in local weather and environment is a crucial issue. Above all, surface corrosion on solar cell multilayers is a major factor that determines the durability of commercial solar cells; corrosive chemical interactions between air, humidity and chemical species and solar cell multilayers can unfavorably affect the durability. Here, we study microscopic and spectroscopic surface techniques to investigate the corrosive interaction on the antireflective layers of solar cell multilayers under various conditions such as acid, base, constant temperature and humidity. Surface morphology and adhesion force were characterized with atomic force microscopy before and after chemical treatment. Chemical composition, and transmittance factors were studied with X-ray photoelectron spectroscopy, and ultraviolet-visible spectroscopy, respectively. Based on these studies, we suggest the dominant factors in the corrosive chemical processes, and their influences on the structural, compositional, and optical properties of the antireflective layers.

  • PDF

Highly conductive and transparent ITO:Zr films for amorphous/crystalline silicon heterojnction solar cell

  • Kim, Yongjun;Hussain, Shahzada Quamar;Kim, Sunbo;Yi, Junsin
    • 한국진공학회:학술대회논문집
    • /
    • 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
    • /
    • pp.296-296
    • /
    • 2016
  • ITO films doped with a small amount of high-permittivity materials not only retain the basic properties of ITO films but also improve some of their properties. We report the highly conductive and transparent (ITO:Zr) films with various substrate (RT to 300oC) temperatures on glass substrate for the HIT solar cell applications. We observed a decrease in sheet resistance from 36 to $11.8{\Omega}/{\Box}$ with the increasing substrate temperature from RT to 300oC, respectively. The ITO:Zr films showed also lowest resistivity of $1.38{\times}10-4{\Omega}.cm$ and high mobility of 42.37cm-3, respectively. The surface and grain boundaries are improved with the increase of substrate temperature as shown by SEM and AFM surface morphologies. The highly conductive and transparent ITO:Zr films were employed as front electrode in HIT solar cell and the best performance of device was found to be Voc = 710 mV, Jsc = 33.70 mA/cm2, FF = 0.742, ${\eta}=17.76%$ at the substrate temperature of $200^{\circ}C$.

  • PDF

락토바실러스 아시도필러스 CBT LA1 생균과 사균체의 세포부착력, 자가응집력, 소수성 상호작용력, LPS 결합력에 대한 평가 (Assessment of cell adhesion, cell surface hydrophobicity, autoaggregation, and lipopolysaccharide-binding properties of live and heat-killed Lactobacillus acidophilus CBT LA1)

  • 신주현;이중수;서재구
    • 미생물학회지
    • /
    • 제51권3호
    • /
    • pp.241-248
    • /
    • 2015
  • 프로바이오틱스에 대한 연구는 주로 생균의 효과가 많이 알려져 있지만 가열 처리된 유산균인 사균체의 기능에 대한 연구도 활발히 이루어지고 있다. 본 연구에서는 락토바실러스 아시도필러스 CBT LA1의 사균체의 장관장벽 기능에 대하여 in vitro, in vivo에서 실험하였다. 이를 위하여, 세포표면 소수성 상호작용력(cell surface hydrophobicity), 자가응집력(autoaggregation), 세포에 부착하는 능력(cell adhesion)과 자가응집력(autoaggregation), LPS와의 결합력을 조사하였다. 또한 HT-29 장상피세포에서 LPS로 유도되는 IL-8의 발현을 억제하는 효과를 조사하였다. CBT LA1을 80도에서 121도까지 10분 동안 열을 처리하였을 때, 80도에서 열을 처리한 CBT LA1 사균체가 가장 높은 세포에 부착하는 능력을 보여 주었다. CBT LA1 생균과 비교했을 때, 80도에서 열을 처리한 CBT LA1 사균체는 높은 LPS와의 결합력, 소수성 상호작용력, 자가응집력, HT-29 세포에 부착하는 능력과 IL-8의 발현을 억제하는 능력을 보여주었다. In vivo 실험에서 FITC로 label된 LPS를 투여하였을 때 16시간 후, CBT LA1 사균체를 섭취한 동물의 장관 내 LPS가 가장 많이 제거되었다. 이러한 연구 결과들은 CBT LA1 생균처럼 CBT LA1 사균체도 장관장벽 기능을 가지며 이는, 파마바이오틱스로서 그 가능성을 시사한다.

고분자전해질 연료전지용 금속분리판의 특성 및 코팅 기술 (Properties and coating technology of metallic bipolar plate for polymer electrolyte fuel cells)

  • 문성모;이수연;권두영
    • 한국표면공학회지
    • /
    • 제55권3호
    • /
    • pp.133-142
    • /
    • 2022
  • This paper reviews bipolar plate materials and coatings for polymer electrolyte fuel cell. First, six roles and 10 requirements of the bipolar plate are described in detail. Secondly, type of materials for the bipolar plate and their advantages and disadvantages are mentioned. Thirdly, different metallic materials are introduced in terms of electrical and thermal conductivities, corrosion resistance, weight, strength and cost. Finally, various types of coating materials and methods were briefly reviewed.

Effect of surface-treatments on flexibility and guided bone regeneration of titanium barrier membrane

  • Kim, Jin-Tae;Kim, Byoung Soo;Jeong, Hee Seok;Heo, Young Ku;Shin, Sang-Wan;Lee, Jeong-Yol;Shim, Young Ho;Lee, Deuk Yong
    • 한국결정성장학회지
    • /
    • 제25권3호
    • /
    • pp.98-104
    • /
    • 2015
  • Titanium barrier membranes are prepared to investigate the effect of surface-treatments, such as machining, electropolishing, anodizing, and electropolishing + TiN coating, on the biocompatibility and physical properties of the membranes. The surface roughness (Ra) of the membrane decreases from machining ($0.37{\pm}0.09{\mu}m$), TiN coating ($0.22{\pm}0.09{\mu}m$), electropolishing ($0.20{\pm}0.03{\mu}m$), to anodizing ($0.15{\pm}0.03{\mu}m$). The highest ductility (24.50 %) is observed for the electropolished Ti membrane. No evidence of causing cell lysis or toxicity is found for the membranes regardless of the surface-treatments. Cell adhesion results of L-929 and MG-63 show that the machined Ti membrane exhibits the highest cell adhesion while the electropolished membrane is the best membrane for the L-929 cell proliferation after 7 days. However, no appreciable difference in MG-63 cell proliferation among variously surface-treated membranes is detected, suggesting that the electropolished Ti membrane is likely to be the best membrane due to the synergic combination of tailored flexibility and excellent fibroblast proliferation.