• 제목/요약/키워드: Cell surface display

검색결과 205건 처리시간 0.032초

바이오접합과 자가결합을 이용한 박테리아 세포막의 위치 특이적 형광 표지 (Site-specific Dye-labeling of the Bacterial Cell Surface by Bioconjugation and Self-assembly)

  • 양이지;임성인
    • Korean Chemical Engineering Research
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    • 제60권3호
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    • pp.398-406
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    • 2022
  • 그람음성균의 외막은 수많은 생물물리학적 및 생화학적 과정이 작용하여 생존력을 유지하도록 설계되어 있는 세포환경의 가장 바깥 층이다. 세포공학의 발전으로 인해 박테리아의 막 환경을 변경하는 등 유전정보를 원하는 대로 조작할 수 있게 되었고 이는 박테리아를 특정 목적에 적용시킬 수 있게 하였다. 그중 기능성 분자를 박테리아 외막에 표지하는 세포 표면공학은 숙주세포가 특정 외부물질이나 자극에 반응하도록 유도하는 전략 중 하나이다. 기능성 펩타이드 또는 단백질을 세포 표면에 표지하기 위한 방법으로 막 고정 모티프를 융합한 후 세포 내에서 발현하는 방법이 일반적으로 사용되고 있지만 이는 박테리아 시스템에서 발현할 수 없는 외인성 단백질이나 크기가 큰 단백질에는 적용할 수 없다는 한계점이 있다. 박테리아 외막의 구성요소에 자연적으로 존재하는 반응성 그룹과 기능성 물질을 화학접합하는 방법도 있으나 필수 구성 요소의 비특이적 변형으로 인해 세포의 생장이 저해되는 경우가 많다. 본 연구에서는 비천연아미노산 또는 자가결합 도메인을 사용해 대장균의 세포 표면을 부위 특이적으로 형광 표지하는 두 가지의 접근법을 수행하였다. 첫 번째 접근법은 화학선택적 반응성을 지닌 비천연아미노산이 삽입된 펩타이드를 대장균 표면에 발현하여 위치 특이적으로 형광염료를 접합시키는 방법이다. 두 번째 접근법은 자가결합능력을 지닌 이종 이량체 코일-코일에서 유래된 α-나선 도메인을 대장균 외막에 발현하고 녹색 형광 단백질이 융합된 상보적인 α-나선 도메인을 막 표면에 특이적으로 고정하는 방법이다. 제시된 방법들은 위치와 시간이 제어된 방식으로 박테리아 외막에 새로운 기능을 부여하는 방법론으로서 유용하다.

이온빔 조사각도에 따른 네마틱 액정의 액정 배향 특성 (LC Orientation Characteristics of NLC on Polyimide Surface According to Ion-beam Irradiation Angles)

  • 이강민;오병윤;박홍규;임지훈;이원규;나현재;김병용;한정민;이상극;서대식
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2008년도 추계학술대회 논문집 Vol.21
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    • pp.329-329
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    • 2008
  • To date, rubbing has been widely used to align LC molecules uniformly. Although rubbing can be simple, it has fundamental problems such as the generation of defects by dust and static electricity, and difficulty in achieving a uniform LC alignment on a large substrate. Therefore, noncontact alignment has been investigated. Ion beam induced alignment method, which provides controllability, nonstop process, and high resolution display. In this study, we investigated liquid crystal (LC) alignment with ion beam (IB) that non contact alignment technique on polyimide and electro-optical characteristics of twisted nematic (TN)-liquid crystal display (LCD) on the poly imide under various ion beam angles. In this experiment, Polyimide layer was coated on glass by spin-coating and Voltage-transmittance(VT) and response time characteristics of the TN cell were measured by a LCD evaluation system. The good characteristics of the nematic liquid crystal (NLC) alignment with the ion beam exposure poly imide surface was observed. The tilt angle of NLC on the PI surface with ion beam exposure can be measured under $1^{\circ}4 for all of irradiation angles. In addition, it can be achieved the good ED properties, and residual DC property of the ion beam aligned TN cell on polyimide surface.

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Ultra High Luminance and Luminous Efficacy MFFL (Mercury-free Flat Fluorescent Lamp) for Scanning Backlights of LCD TVs

  • Seo, In-Woo;Jung, Jae-Chul;Oh, Byung-Joo;Whang, Ki-Woong
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2009년도 9th International Meeting on Information Display
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    • pp.231-234
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    • 2009
  • In order to realize the high luminance and luminous efficacy MFFLs for LCD backlights, we optimized the phosphor profile to enlarge the surface area. The MFFL with the new phosphor profile shows a very wide luminance range from 2,600 to 17,000 nit with the corresponding luminous efficacy from 66 to 32.5 lm/W. The results were obtained with the color coordinate of the phosphor to be around (0.25, 0.23). And the single cell is expanded into a multi-structured configuration to realize a 32 inch-sized lamp by a simple repetition of the single cells, and a new driving scheme is proposed for an adaptive local dimming and scanning drive using dual auxiliary electrodes and bipolar drive scheme. Especially the ultra high luminance and luminous efficacy results suggest the duty time of illumination of the backlight unit can be significantly reduced.

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Development of 2-inch Plastic Film STN LCD

  • Park, Sung-Kyu;Han, Jeong-In;Kim, Won-Keun;Kwak, Min-Gi
    • Journal of Information Display
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    • 제1권1호
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    • pp.14-19
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    • 2000
  • Due to distinct properties of plastic substrates such as poor thermal resistance, non-rigidness and high thermal expansion, it is difficult to fabricate plastic film LCDs by conventional LCD processes. Poor thermal resistance and high thermal expansion of substrates induced deformation of substrates surface, mismatch of thermal expansion between ITO electrodes and substrates resulted in defects in the ITO electrodes during the high temperature process. Defects of ITO electrodes and non-uniform cell gap caused by non-rigid and flexible properties were also observed in the pressuring process. Based on in these observations, we used a newly developed material and fabrication process to prevent deformation of substrates, defects of electrodes and to maintain uniform cell gap. The maximum temperature of the process is limited up to $110^{\circ}C$ and pressure loaded during the process is five times less than conventional one. With these invented processes and materials, we obtained highly reliable Plastic Film STN LCDs whose electro-optical characteristics are better than or equivalent to those of typical glass LCDs.

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Si 태양전지 금속배선 공정을 위한 나노 Ag 잉크젯 프린터 제작 및 응용 (Manufacturing of Ag Nano-particle Ink-jet Printer and the Application into Metal Interconnection Process of Si Solar Cells)

  • 이정택;최재호;김기완;신명선;김근주
    • 반도체디스플레이기술학회지
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    • 제10권2호
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    • pp.73-81
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    • 2011
  • We manufactured the inkjet printing system for the application into the nano Ag finger line interconnection process in Si solar cells. The home-made inkjet printer consists of motion part for XY motion stage with optical table, head part, power and control part in the rack box with pump, and ink supply part for the connection of pump-tube-sub ink tanknozzle. The ink jet printing system has been used to conduct the interconnection process of finger lines on Si solar cell. The nano ink includes the 50 nm-diameter. Ag nano particles and the viscosity is 14.4 cP at $22^{\circ}C$. After processing of inkjet printing on the finger lines of Si solar cell, the nano particles were measured by scanning electron microscope. After the heat treatment at $850^{\circ}C$, the finger lines showed the smooth surface morphology without micropores.

A Novel ECB cell using bend structure for Nematic Liquid Crystal on Rubbed Polyimide Surface

  • Hwang, J.Y.;Kim, K.W.;Seo, D.S.
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2004년도 Asia Display / IMID 04
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    • pp.1137-1141
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    • 2004
  • In this paper, we have improved a novel ECB mode using bend structure with high tilt angle in the unique condition by Hot plate equipment. The new control of tilt angle for nematic liquid crystal (NLC) with negative and positive dielectric anisotropy on the rubbed homeotropic polyimide (PI) using baking method by Hot plate equipment was investigated. LC tilt angle decreased with increasing baking temperature and time. We suggest that the development of the novel ECB cell using control of tilt angle on the homeotropic surface is a promising technique for the achievement of fast response time and high contrast ratio.

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프로젝션 TV 적용을 위한 액정 디스플레이의 열적 및 UV 안전성에 관한 연구 (Study on thermal and UV stability of Liquid Crystal Display for Projection TV Application)

  • 최성호;황정연;배유한;이휘원;서대식
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2005년도 추계학술대회 논문집 Vol.18
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    • pp.287-288
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    • 2005
  • In this study, we have investigated electro-optical characteristics of thermal and UV stressed TN cells on the rubbed polyimide surface. Mono-domain alignments of thermal stressed TN cells over temperature of liquid crystal isotropic phase were almost same that of no thermal stressed TN cells. Also, threshold voltage and response time of thermal stressed TN cells were same that of no thermal stressed TN cells. Finally, the residual DC voltage of the thermal stressed TN cell on the polyimide surface show decrease of characteristics as increasing thermal stress time. Therefore, thermal stability of TN cell was decreased by high thermal stress for the long times.

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바람 증폭 장치를 가진 태양전지 오염방지시스템의 TRIZ(6SC) 응용 설계 (A Study on TRIZ Applied Design for Contamination Preventation System of Solar Battery with Wind Amplifier)

  • 허용정;홍성도;김재민
    • 반도체디스플레이기술학회지
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    • 제12권2호
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    • pp.7-11
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    • 2013
  • This paper presents a study on the design for contamination preventation system of solar battery. The system of contamination preventation has an wind amplifier. Wind amplifier is conceptually designed by using TRIZ. The surface of solar battery is covered with dust during accumulate the energy. The dust cause reduce power of photovoltaic module and efficiency drop of photovoltaic system. Reflect and absorb of incident ray are caused by the dust on surface of solar battery. The solution of this problem has been derived using 6SC(6 steps creativity)TRIZ. The wind amplifier which has structure such as funnel shape. The incident wind in the wide hole is amplified by the gate become narrow. The system of contamination preventation with wind amplifier which mounted on the side of the solar battery surface reduces the reflect and absorb and improve the efficiency of photovoltaic system.

DLC 박막을 이용한 Ion Beam 배향 TN 셀의 전기광학특성에 관한 연구 (A Study on Electro-Optical Characteristics of the Ion Beam Aligned TN Cell on the DLC Thin Film)

  • 황정연;조용민;노순준;이대규;백홍구;서대식
    • 한국전기전자재료학회논문지
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    • 제15권8호
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    • pp.726-730
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    • 2002
  • Electro-optical (EO) performances of the ion beam (IB) aligned twisted-nematic (TN)-liquid crystal display (LCD) with ion beam exposure on the new diamond-like carbon (DLC) thin film surface were investigated. A good voltage-transmittance (V-T) curve of the ion beam aligned TN-LCD with oblique ion beam exposure on the DLC thin film surface for 1 min was observed. Also, the fast response time of the ion beam aligned TN-LCD with oblique ion beam exposure on the DLC thin film surface for 1 min can be achieved. Finally, the residual DC voltage of the ion beam aligned TN-LCD on the DLC thin film surface is almost the same as that of the rubbing aligned TN-LCD on a polyimide (Pl) surface.

Electro-Optical Performances of In plane Switching (IPS) Cell on the Inorganic Thin Film by Ion Beam (IB) Method

  • Kim, Sang-Hoon;Hwang, Jeoung-Yeon;Kim, Jong-Hwan;Han, Jung-Min;Seo, Dae-Shik;Kim, Sung-Yeon;Oh, Byeong-Yun;Myoung, Jae-Min
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2006년도 6th International Meeting on Information Display
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    • pp.796-799
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    • 2006
  • We studied the nematic liquid crystal (NLC) alignment capability by the Ion beam (IB) alignment method on a NDLC (Nitrogen doped Diamond Like Carbon) as a C:H thin film, and investigated electro-optical (EO) performances of the IB aligned In plane switching (IPS) cell with NDLC surface. A good LC alignment by IB exposure on a NDLC surface was achieved. Monodomain alignment of the IB aligned IPS cell can be observed. The good electro-optical characteristics of the IB aligned IPS cell was observed with oblique IB exposure on the NDLC as a-C:H thin film for 1min.

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