• Title/Summary/Keyword: Cell size

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Ultrastructural and Histochemical Study on the Prostate Gland of a Korean Slug, Incilaria fruhstorferi (한국산 산민달팽이 (Incilaria fruhstorferi) 전립선의 미세구조 및 조직화학적 연구)

  • Chang, Nam-Sub
    • Applied Microscopy
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    • v.27 no.2
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    • pp.155-163
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    • 1997
  • Ultrastructural and histochemical studies were carried out on the prostate gland of a Korean slug (Incilaria fruhstorferi) with light and electron microscopes. The results are as follows. The endothelial tissue of the prostate gland is constituted with tall and narrow ciliated columnar cells, irregularly shaped cells and gland cells in which are included the elongated oval or ellipsoid nucleus those are surrounded by curved membrane. The endothelial tissue of the prostate gland is composed by type-A, B, C and type-D gland cells, and the glanules of type-A, B, and D gland cells are certified to neutral mucopolysaccharide because are related by PAS-alcian blue (pH 2.5) , but the other hand type-C gland cell is only weakly reacted to PAS, but strongly reacted by Million reaction. The glanules of type-A gland cell are small size (about $0.4{\mu}m$) and are seen high electron density, but the glanules of type-B gland cell are large size (about $0.7{\mu}m$) and glanule density is same type-A glanules. Long ellipsoid type-C gland cell contained round nucleus which is well developed beterochromatin in, and that small oval glanules (size, about $0.9{\mu}m$) of moderate high electron density which are formed a group of large glanule together with $4\sim5ea$, but type-D gland cell possessed round small nucleus are seen high electron dense glanules (size, $0.8{\mu}m$).

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A Study on the Computation of Curve Number Using GIS (GIS를 이용한 CN 산정에 관한 연구)

  • Cho, Yong-Jae;Park, Sang-Ju;Jeong, In-Ju;Kim, Sang-Yong
    • Journal of Korean Society for Geospatial Information Science
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    • v.11 no.3 s.26
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    • pp.47-53
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    • 2003
  • Recently, there is studying about slope analysis according to cell size and affect in conformity to determination of hydrologic topographical parameters the cell size a classified map scale about subwatershed. In this study, we wish to offer the base data to determination of hydrologic topographical parameters request of runoff model analysis in this basin on the basis of this results that we compute the CN(curve number) using GIS after classify the map of soil and landuse on the Su-Young River basin. Also, as determination a classified cell size of $100m{\times}100m$ in case of the most optimum size.

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Comparison of Cell and Nuclear Size Difference between Diploid and Induced Triploid in Marine Medaka, Oryzias dancena

  • Goo, In Bon;Im, Jae Hyun;Gil, Hyun Woo;Lim, Sang Gu;Park, In-Seok
    • Development and Reproduction
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    • v.19 no.3
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    • pp.127-134
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    • 2015
  • The influence of triploidization on cell and nucleus size characteristics of the same tissues of erythrocyte, retina, kidney, hepatocyte and midgut epithelium in marine medaka, Oryzias dancena has been determined histologically. Induced triploid fish are produced by cold shock treatments. Likewise, the size of horizontal cell nucleus in inner nuclear layer of retina, ganglion cell nucleus in ganglion cell layer of retina, proximal tubule cell of kidney, hepatocytes and nuclear height of midgut epithelium all appear to be significantly larger than diploid (P<0.05). On the other hand, retina thickness is larger in diploid than induced triploid (P<0.05). Induced triploid shows low density of cell number. Results of this study suggest that same characteristics in the induced triploid exhibiting larger cells and nucleus sizes with fewer number of cells than the diploid can be useful criteria for the distinction between diploid and induced triploid, and also the ploidy level in marine medaka.

Establishment of bovine Fetal Fibroblasts Line for Production of Cloned Calves in Korean Native Cattle: The Effects of Culture Period and Various Cell Size on the Efficiency of Nuclear Transfer (복제 한우 생산을 위한 Bovine Fetal Fibroblasts의 이용에 관한 연구: 공여핵원의 배양기간 및 세포 크기가 핵이식의 효율에 미치는 영향)

  • 황우석;박종임;조종기;김기연;신수정;용환율;이병천
    • Journal of Embryo Transfer
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    • v.14 no.2
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    • pp.93-97
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    • 1999
  • The development potential of bovine somatic cells was evaluated using nuclear transfer. A single donor cell derived from fetus of HanWoo(Korean Native Cattle) was selected and deposited into perivitelline space of each enucleated oocyte before electrical fusion and activation. Nuclei of donor cells starved for 7 days (37%) tended to support the development of reconstitute embryo the blastocyst stage better than those of donor cells starved 3, 14 and 30 days. The cleavage rate was significantly lower(P<0.05) in reconstitute embryos derived from large size donor cells(51.2%), than those from small medium size donor cells(76.6 and 73.5, respectively). The developmental rate to blastocyst of reconstructed embryos from medium size donor cells was higher than those from small and medium size donor cells. This study demonstrates that an appropriate culture period for induction into quiescent stage and the size of donor cells effect on the efficiency of nuclear transfer using cultured bovine cells.

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Mass Production and Accumulation Characteristics of Polyhydroxyalkanoates by Fed-batch culture of Alcaligenes eutrophus under Phosphate Limitation (인산염 제한하에서 Alcaligenes eutrophus의 유가식 배양에 의한 Polyhydroxyalkanoates의 대량 산과 축적특성)

  • 류희욱;조경숙;장용근
    • KSBB Journal
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    • v.13 no.2
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    • pp.187-194
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    • 1998
  • For mass production of polyhydroxyalkanoates (PHA), high cell density cultures of Alcaligenes eutrophus by fed-batch culture under phosphate-limitation condition has been investigated. PHA accumulation by the regulation by the regulation of initial phosphate concentration could be automatically induced, and high density cell culture above 200 g/L also could be successfully produced. The production of Poly-$\beta$-hydroxybutyrate (PHB) and dry cell weight increased with increasing the initial phosphate concentration. When the initial concentrations of phosphate were in the ranges of 1.5~4.5 g-PO$_4$/L, PHB and dry cell weight obtained were 83~266 g/L and 61~216 g/L, respectively, and PHB productivity was in the ranges of 1.35~3.10 g/L.h. When a mixture of glucose and propionic acid is used as carbon sources, poly(3-hydroxybutyrate-co-poly-3-hydroxyvalerate), P(3HB-co-3HV), could be also successfully produced under phosphate limitation condition. When the mole ratio of propionic acid to glucose in the feeding solution is 0.22, a final dry cell weight of 150 g/L and a P(3-HB-co-3HV) of 90 g/L were produced. Morphological changes and size distribution of PHB granules synthesized in A. eutrophus under phosphate-limitation condition are determined by TEM during the course of fed-batch. Mean granule diameters of PHB produced are in the range of 0.36~0.39 $\mu$m, and mean cell size was elongated from 0.54~0.59 $\mu$m$\times$ 1.3~1.5 $\mu$m to 0.83~0.89 $\mu$m $\times$2.0~2.3 $\mu$m. Phosphate concentration in media did not affect size distribution of PHB granule and cell.

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Development and Evaluation of SWAT Topographic Feature Extraction Error(STOPFEE) Fix Module from Low Resolution DEM (저해상도 DEM 사용으로 인한 SWAT 지형 인자 추출 오류 개선 모듈 개발 및 평가)

  • Kim, Jong-gun;Park, Youn-shik;Kim, Nam-won;Chung, Il-moon;Jang, Won-seok;Park, Jun-ho;Moon, Jong-pil;Lim, Kyoung Jae
    • Journal of Korean Society on Water Environment
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    • v.24 no.4
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    • pp.488-498
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    • 2008
  • Soil and Water Assessment Tool (SWAT) model have been widely used in simulating hydrology and water quality analysis at watershed scale. The SWAT model extracts topographic feature using the Digital Elevation Model (DEM) for hydrology and pollutant generation and transportation within watershed. Use of various DEM cell size in the SWAT leads to different results in extracting topographic feature for each subwatershed. So, it is recommended that model users use very detailed spatial resolution DEM for accurate hydrology analysis and water quality simulation. However, use of high resolution DEM is sometimes difficult to obtain and not efficient because of computer processing capacity and model execution time. Thus, the SWAT Topographic Feature Extraction Error (STOPFEE) Fix module, which can extract topographic feature of high resolution DEM from low resolution and updates SWAT topographic feature automatically, was developed and evaluated in this study. The analysis of average slope vs. DEM cell size revealed that average slope of watershed increases with decrease in DEM cell size, finer resolution of DEM. This falsification of topographic feature with low resolution DEM affects soil erosion and sediment behaviors in the watershed. The annual average sediment for Soyanggang-dam watershed with DEM cell size of 20 m was compared with DEM cell size of 100 m. There was 83.8% difference in simulated sediment without STOPFEE module and 4.4% difference with STOPFEE module applied although the same model input data were used in SWAT run. For Imha-dam watershed, there was 43.4% differences without STOPFEE module and 0.3% difference with STOPFEE module. Thus, the STOPFEE topographic database for Soyanggang-dam watershed was applied for Chungju-dam watershed because its topographic features are similar to Soyanggang-dam watershed. Without the STOPFEE module, there was 98.7% difference in simulated sediment for Chungju-dam watershed for DEM cell size of both 20 m and 100 m. However there was 20.7% difference in simulated sediment with STOPFEE topographic database for Soyanggang-dam watershed. The application results of STOPFEE for three watersheds showed that the STOPFEE module developed in this study is an effective tool to extract topographic feature of high resolution DEM from low resolution DEM. With the STOPFEE module, low-capacity computer can be also used for accurate hydrology and sediment modeling for bigger size watershed with the SWAT. It is deemed that the STOPFEE module database needs to be extended for various watersheds in Korea for wide application and accurate SWAT runs with lower resolution DEM.

Effect of cutting time, media and plug cell size on rooting of Coreopsis spp and Dianthus spp. (Coreopsis spp와 Dianthus spp의 삽목 시 삽목시기, 용토 및 프러그 셀 크기가 발근에 미치는 영향)

  • Park, N.B.;Shin, Y.K.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.12 no.1
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    • pp.39-45
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    • 2010
  • This study was carried out to investigate the growth and rooting of cutting time, media and plug cell size in Dianthus spp and Coreopsis spp. A rooting rate was the low in lated rooting time rooting rate was the over 90% in 20th May and 20th August otherwise was the 82% and 87% in 20th September. Media of rooting was the over the 90% in all treatment media and sand media was low. Number of rooting was the best in peatmoss(1)+perlite(1) media. The size of plug cell was the 98% in 162 plug cell in Coreopsis spp. The 162-200 plug cell size was the 95.8-96% in Dianthus spp.

Effect of Frozen Storage Temperature on the Quality of Premium Ice Cream

  • Park, Sung Hee;Jo, Yeon-Ji;Chun, Ji-Yeon;Hong, Geun-Pyo;Davaatseren, Munkhtugs;Choi, Mi-Jung
    • Food Science of Animal Resources
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    • v.35 no.6
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    • pp.793-799
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    • 2015
  • The market sales of premium ice cream have paralleled the growth in consumer desire for rich flavor and taste. Storage temperature is a major consideration in preserving the quality attributes of premium ice cream products for both the manufacturer and retailers during prolonged storage. We investigated the effect of storage temperature (−18℃, −30℃, −50℃, and −70℃) and storage times, up to 52 wk, on the quality attributes of premium ice cream. Quality attributes tested included ice crystal size, air cell size, melting resistance, and color. Ice crystal size increased from 40.3 µm to 100.1 µm after 52 wk of storage at −18℃. When ice cream samples were stored at −50℃ or −70℃, ice crystal size slightly increased from 40.3 µm to 57-58 µm. Initial air cell size increased from 37.1 µm to 87.7 µm after storage at −18℃ for 52 wk. However, for storage temperatures of −50℃ and −70℃, air cell size increased only slightly from 37.1 µm to 46-47 µm. Low storage temperature (−50℃ and −70℃) resulted in better melt resistance and minimized color changes in comparison to high temperature storage (−18℃ and −30℃). In our study, quality changes in premium ice cream were gradually minimized according to decrease in storage temperature up to−50℃. No significant beneficial effect of −70℃ storage was found in quality attributes. In the scope of our experiment, we recommend a storage temperature of −50℃ to preserve the quality attributes of premium ice cream.

Interaction fields based on incompatibility tensor in field theory of plasticity-Part II: Application-

  • Hasebe, Tadashi
    • Interaction and multiscale mechanics
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    • v.2 no.1
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    • pp.15-30
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    • 2009
  • The theoretical framework of the interaction fields for multiple scales based on field theory is applied to one-dimensional problem mimicking dislocation substructure sensitive intra-granular inhomogeneity evolution under fatigue of Cu-added steels. Three distinct scale levels corresponding respectively to the orders of (A)dislocation substructures, (B)grain size and (C)grain aggregates are set-up based on FE-RKPM (reproducing kernel particle method) based interpolated strain distribution to obtain the incompatibility term in the interaction field. Comparisons between analytical conditions with and without the interaction, and that among different cell size in the scale A are simulated. The effect of interaction field on the B-scale field evolution is extensively examined. Finer and larger fluctuation is demonstrated to be obtained by taking account of the field interactions. Finer cell size exhibits larger field fluctuation whereas the coarse cell size yields negligible interaction effects.

Emulsion rheology and properties of polymerized high internal phase emulsions

  • Lee, Seong-Jae
    • Korea-Australia Rheology Journal
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    • v.18 no.4
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    • pp.183-189
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    • 2006
  • High internal phase emulsions are highly concentrated emulsion systems consisting of a large volume of dispersed phase above 0.74. The rheological properties of high internal phase water-in-oil emulsions were measured conducting steady shear, oscillatory shear and creep/recovery experiments. It was found that the yield stress is inversely proportional to the drop size with the exponent of values between 1 and 2. Since the oil phase contains monomeric species, microcellular foams can easily be prepared from high internal phase emulsions. In this study, the microcellular foams combining a couple of thickeners into the conventional formulation of styrene and water system were investigated to understand the effect of viscosity ratio on cell size. Cell size variation on thickener concentration could be explained by a dimensional analysis between the capillary number and the viscosity ratio. Compression properties of foam are important end use properties in many practical applications. Crush strength and Young's modulus of microcellular foams polymerized from high internal phase emulsions were measured and compared from compression tests. Of the foams tested in this study, the foam prepared from the organoclay having reactive group as an oil phase thickener showed outstanding compression properties.