Mass Production and Accumulation Characteristics of Polyhydroxyalkanoates by Fed-batch culture of Alcaligenes eutrophus under Phosphate Limitation

인산염 제한하에서 Alcaligenes eutrophus의 유가식 배양에 의한 Polyhydroxyalkanoates의 대량 산과 축적특성

  • 류희욱 (숭실대학교 환경·화학공학과) ;
  • 조경숙 (이화여자대학교 환경공학과) ;
  • 장용근 (한국과학기술원 화학공학과 및 생물공정연구센터)
  • Published : 1998.04.01

Abstract

For mass production of polyhydroxyalkanoates (PHA), high cell density cultures of Alcaligenes eutrophus by fed-batch culture under phosphate-limitation condition has been investigated. PHA accumulation by the regulation by the regulation of initial phosphate concentration could be automatically induced, and high density cell culture above 200 g/L also could be successfully produced. The production of Poly-$\beta$-hydroxybutyrate (PHB) and dry cell weight increased with increasing the initial phosphate concentration. When the initial concentrations of phosphate were in the ranges of 1.5~4.5 g-PO$_4$/L, PHB and dry cell weight obtained were 83~266 g/L and 61~216 g/L, respectively, and PHB productivity was in the ranges of 1.35~3.10 g/L.h. When a mixture of glucose and propionic acid is used as carbon sources, poly(3-hydroxybutyrate-co-poly-3-hydroxyvalerate), P(3HB-co-3HV), could be also successfully produced under phosphate limitation condition. When the mole ratio of propionic acid to glucose in the feeding solution is 0.22, a final dry cell weight of 150 g/L and a P(3-HB-co-3HV) of 90 g/L were produced. Morphological changes and size distribution of PHB granules synthesized in A. eutrophus under phosphate-limitation condition are determined by TEM during the course of fed-batch. Mean granule diameters of PHB produced are in the range of 0.36~0.39 $\mu$m, and mean cell size was elongated from 0.54~0.59 $\mu$m$\times$ 1.3~1.5 $\mu$m to 0.83~0.89 $\mu$m $\times$2.0~2.3 $\mu$m. Phosphate concentration in media did not affect size distribution of PHB granule and cell.

Keywords

References

  1. Mirobiol. Rev. v.54 Occurrence, Metabolism, Metabolic Role, and Industrial uses of Bacterial Polyhydroxyalkanoates Anderson,A.J.;E.A.Dawes
  2. Emerging Technologies in Plastics Recycling Recycled Plastics: Product Applications and Potential Bennett,R.A.;G.D.Andrews(ed.);P.M.Subramanian(ed.)
  3. Trends in Biotechnol. v.5 Polymer Synthesis by Microorganisms: Technology and Economics Byrom,D.
  4. Biotechnol. Lett. v.14 Production of Poly-β-hydroxybutyrate by Fed-Batch Culture of Recombinant Escherichia coli Kim,B.S.;S.Y.Lee;H.N.Chang
  5. Biotechnol. Bioeng. v.43 Production of Poly-3-hydroxybutyric Acid by Ffed-Batch Culture of Alcaligenes eutrophus with Glucose Cconcentration Control Kim,B.S.;S.C.Lee;S.Y.Lee;H.N.Chang;Y.K.Chang;S.I.Woo
  6. Enzyme Microbiol. Technol. v.16 Production of 3-Hydroxybutyric-co-3-Hydroxyvaleric Acid by Fed-Batch Culture of Alcaligenes eutrophus with Substrate Control using On-line Glucose Analyzer Kim,B.S.;S.C.Lee;S.Y.Lee;H.N.Chang;Y.K.Chang;S.I.Woo
  7. Biotechnol. Lett. v.18 High Production of Poly-β-Hydroxybutyrate (PHB) from Methylobacterium organophilum under Potassium Limitation Kim,S.W.;P.Kim;H.S.Lee;J.H.Kim
  8. Biomaterials: Novel Materials from Biological Sources Byrom,D.
  9. Makromolecular Chem. Rapid Commun. v.8 Biosynthesis of Terpolyesters of 3-Hydroxybutyrate, 3-Hydroxyvalerate, and 5-Hydroxyvalerate in Alcaligenes eutrophus from 5-Chloropentanoic and Pentanoic Acids Doi,Y.;K.M.Tamaki;K.Soga
  10. Appl. Microbiol. Biotechnol. v.28 Production of Copolyesters of 3-Hydroxybutyrate and Pentanoic Acids Doi,Y.;A.Tamaki;M.Kunioka;K.Soga
  11. Appl. Microbiol. Biotechnol. v.30 Production of Biodegradable Copolyesters of 3-Hydroxybutyrate and 4-Hydroxybutyrate by Alcaligenes eutrophus Doi,Y.;M.Kunioka;Y.Kawaguchi
  12. Biotechnol. Bioeng. v.44 Effect of Alcohol Feeding Mode on the Biosynthesis of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Park,C.H.;V.K.Damodaran
  13. Biotechnol. Prog v.10 Biosynthesis of Poly-3-hydroxybutyrate-co-3-hydroxyvalerate from Ethanol and Pentanol by Alcaligenes eutrophus Park,C.H.;V.K.Damodaran
  14. Biotechnol. Lett. v.19 Poly(hydroxybutyrate-co-hydroxyvalerate) from Swine Waste Liquor by Azotobater vinelandii UWD Cho,K.S.;H.W.Ryu;C.H.Park;P.R.Goodrich
  15. Appl. Microbiol. Biotechnol. v.31 Production of Poly-β-hydroxybutyrate by Azotobacter vinelandii Strain UWD during Growth on Molasses and Other Complex Carbon Sources Page,W.J.
  16. Appl. Environ. Microbiol. v.58 Formation of Poly(hydroxybutyrate-co-hydroxyvalerate) by Azotobacter vinelandii UWD Page,W.J.;J.Manchak;B.Rudy
  17. Appl. Microbiol. Biotechnol. v.23 Mass Production of Poly-β-hydroxybutyric Acid by Fully Automatic Fed-Batch Culture of Methylotroph Suzuki,T.;T.Yamane;S.Shimizu
  18. Biotechnol. Bioeng. v.41 High Cell Density Cultivation of Psedomonas olevorans: Growth and Production of Poly(3-hydroxybutyrates) in Two-liquid phase Batch and Fed-batch Systems Preusting,H.;R.van Houten;A.Hoefs;E.K.van Langenberghe;O.Favre-Bulle;B.Witholt
  19. Appl. Microbiol. Biotechnol. v.54 Formation of Poyesters by Pseudomonas oleovorans: Effect of Substrates on Formation and Composition of Poly-(R)-3-hydroxyalka noates and Poly-(R)-3-hydroxyalkanoates Lageveen,R.G.;G.W.Huisman;H.Preusting;P.Ketelaar;G.Eggink;B.Witholt
  20. Biotechnol. Prog. v.1 High Cell Cultivation of Pseudomonas oleovorans for the Production of Poly(3-Hydroxyalkanoates) Lee,S.Y.
  21. J. Bacteriol. v.32 Construction of Plasmids, Estimation of Plasmid Stability, and Use of Stable Plasmids for the Production of Poly-3-hydroxybutyric Acid by Recombinant Escherichia coli Lee,S.Y.;H.N.Chang;Y.K.Chang
  22. Biotechnol. Lett. v.17 Size Analysis of Poly(3-hydroxybutyric Acid) Granules Produced in Recombinant Escherichia coli Middelberg,A.P.J.;S.Y.Lee;J.Martin;D.R.G.Williams;H.N.Chang
  23. Synthesis of Poly-3-hydroxyalkanoates is a Common Feature of Fluorescent Pseudomonas v.55 Huisman,G.W.;O.De Leeuw;G.Eggink;B.Witholt
  24. Biotechnol. Bioeng. v.44 Comparison of Recombinant Escherichia coli Strains for Synthesis and Accumulation of Poly(3-hydroxybutyric Acid) and Morphological Changes Lee,S.Y.;K.M.Lee;H.N.Chang;A.Steinbuchel
  25. Biotechnology v.6b Microbial Production of Poly-β-hydroxybutyric Acid Lafferty,R.M.;B.Korsatko;W.Korsatko;Rehm,H.J.(ed.);G.Reed(ed.)