• Applied Microscopy
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• 제27권2호
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• pp.155-163
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• 1997

Ultrastructural and histochemical studies were carried out on the prostate gland of a Korean slug (Incilaria fruhstorferi) with light and electron microscopes. The results are as follows. The endothelial tissue of the prostate gland is constituted with tall and narrow ciliated columnar cells, irregularly shaped cells and gland cells in which are included the elongated oval or ellipsoid nucleus those are surrounded by curved membrane. The endothelial tissue of the prostate gland is composed by type-A, B, C and type-D gland cells, and the glanules of type-A, B, and D gland cells are certified to neutral mucopolysaccharide because are related by PAS-alcian blue (pH 2.5) , but the other hand type-C gland cell is only weakly reacted to PAS, but strongly reacted by Million reaction. The glanules of type-A gland cell are small size (about $0.4{\mu}m$) and are seen high electron density, but the glanules of type-B gland cell are large size (about $0.7{\mu}m$) and glanule density is same type-A glanules. Long ellipsoid type-C gland cell contained round nucleus which is well developed beterochromatin in, and that small oval glanules (size, about $0.9{\mu}m$) of moderate high electron density which are formed a group of large glanule together with $4\sim5ea$, but type-D gland cell possessed round small nucleus are seen high electron dense glanules (size, $0.8{\mu}m$).

• 대한공간정보학회지
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• 제11권3호
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• pp.47-53
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• 2003

격자크기에 따른 경사도 분석과 소유역에 대하여 축척별 격자크기(cell size)가 수문인자 결정에 어떠한 영향을 미치는지 연구하고자, 본 연구에서는 수영강 유역의 토지이용도 및 토양도를 분류 선정한 후 GIS를 이용하여 CN을 계산하고, 이 값을 이용하여 수영강 유역의 유출모형을 분석하는데 요구되는 수문학적 지형인자의 결정에 기초자료로 활용하고자 한다. 각 격자크기에 따라 CN을 산정한 결과, 본 연구에서는 격자크기가 $100m{\times}100m$인 경우 가장 최적인 것으로 나타났다.

• 한국발생생물학회지:발생과생식
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• 제19권3호
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• pp.127-134
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• 2015

The influence of triploidization on cell and nucleus size characteristics of the same tissues of erythrocyte, retina, kidney, hepatocyte and midgut epithelium in marine medaka, Oryzias dancena has been determined histologically. Induced triploid fish are produced by cold shock treatments. Likewise, the size of horizontal cell nucleus in inner nuclear layer of retina, ganglion cell nucleus in ganglion cell layer of retina, proximal tubule cell of kidney, hepatocytes and nuclear height of midgut epithelium all appear to be significantly larger than diploid (P<0.05). On the other hand, retina thickness is larger in diploid than induced triploid (P<0.05). Induced triploid shows low density of cell number. Results of this study suggest that same characteristics in the induced triploid exhibiting larger cells and nucleus sizes with fewer number of cells than the diploid can be useful criteria for the distinction between diploid and induced triploid, and also the ploidy level in marine medaka.

• 한국수정란이식학회지
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• 제14권2호
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• pp.93-97
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• 1999

The development potential of bovine somatic cells was evaluated using nuclear transfer. A single donor cell derived from fetus of HanWoo(Korean Native Cattle) was selected and deposited into perivitelline space of each enucleated oocyte before electrical fusion and activation. Nuclei of donor cells starved for 7 days (37%) tended to support the development of reconstitute embryo the blastocyst stage better than those of donor cells starved 3, 14 and 30 days. The cleavage rate was significantly lower(P<0.05) in reconstitute embryos derived from large size donor cells(51.2%), than those from small medium size donor cells(76.6 and 73.5, respectively). The developmental rate to blastocyst of reconstructed embryos from medium size donor cells was higher than those from small and medium size donor cells. This study demonstrates that an appropriate culture period for induction into quiescent stage and the size of donor cells effect on the efficiency of nuclear transfer using cultured bovine cells.

• KSBB Journal
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• 제13권2호
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• pp.187-194
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• 1998

For mass production of polyhydroxyalkanoates (PHA), high cell density cultures of Alcaligenes eutrophus by fed-batch culture under phosphate-limitation condition has been investigated. PHA accumulation by the regulation by the regulation of initial phosphate concentration could be automatically induced, and high density cell culture above 200 g/L also could be successfully produced. The production of Poly-$\beta$-hydroxybutyrate (PHB) and dry cell weight increased with increasing the initial phosphate concentration. When the initial concentrations of phosphate were in the ranges of 1.5~4.5 g-PO$_4$/L, PHB and dry cell weight obtained were 83~266 g/L and 61~216 g/L, respectively, and PHB productivity was in the ranges of 1.35~3.10 g/L.h. When a mixture of glucose and propionic acid is used as carbon sources, poly(3-hydroxybutyrate-co-poly-3-hydroxyvalerate), P(3HB-co-3HV), could be also successfully produced under phosphate limitation condition. When the mole ratio of propionic acid to glucose in the feeding solution is 0.22, a final dry cell weight of 150 g/L and a P(3-HB-co-3HV) of 90 g/L were produced. Morphological changes and size distribution of PHB granules synthesized in A. eutrophus under phosphate-limitation condition are determined by TEM during the course of fed-batch. Mean granule diameters of PHB produced are in the range of 0.36~0.39 $\mu$m, and mean cell size was elongated from 0.54~0.59 $\mu$m$\times$ 1.3~1.5 $\mu$m to 0.83~0.89 $\mu$m $\times$2.0~2.3 $\mu$m. Phosphate concentration in media did not affect size distribution of PHB granule and cell.

• 한국물환경학회지
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• 제24권4호
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• pp.488-498
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• 2008

Soil and Water Assessment Tool (SWAT) model have been widely used in simulating hydrology and water quality analysis at watershed scale. The SWAT model extracts topographic feature using the Digital Elevation Model (DEM) for hydrology and pollutant generation and transportation within watershed. Use of various DEM cell size in the SWAT leads to different results in extracting topographic feature for each subwatershed. So, it is recommended that model users use very detailed spatial resolution DEM for accurate hydrology analysis and water quality simulation. However, use of high resolution DEM is sometimes difficult to obtain and not efficient because of computer processing capacity and model execution time. Thus, the SWAT Topographic Feature Extraction Error (STOPFEE) Fix module, which can extract topographic feature of high resolution DEM from low resolution and updates SWAT topographic feature automatically, was developed and evaluated in this study. The analysis of average slope vs. DEM cell size revealed that average slope of watershed increases with decrease in DEM cell size, finer resolution of DEM. This falsification of topographic feature with low resolution DEM affects soil erosion and sediment behaviors in the watershed. The annual average sediment for Soyanggang-dam watershed with DEM cell size of 20 m was compared with DEM cell size of 100 m. There was 83.8% difference in simulated sediment without STOPFEE module and 4.4% difference with STOPFEE module applied although the same model input data were used in SWAT run. For Imha-dam watershed, there was 43.4% differences without STOPFEE module and 0.3% difference with STOPFEE module. Thus, the STOPFEE topographic database for Soyanggang-dam watershed was applied for Chungju-dam watershed because its topographic features are similar to Soyanggang-dam watershed. Without the STOPFEE module, there was 98.7% difference in simulated sediment for Chungju-dam watershed for DEM cell size of both 20 m and 100 m. However there was 20.7% difference in simulated sediment with STOPFEE topographic database for Soyanggang-dam watershed. The application results of STOPFEE for three watersheds showed that the STOPFEE module developed in this study is an effective tool to extract topographic feature of high resolution DEM from low resolution DEM. With the STOPFEE module, low-capacity computer can be also used for accurate hydrology and sediment modeling for bigger size watershed with the SWAT. It is deemed that the STOPFEE module database needs to be extended for various watersheds in Korea for wide application and accurate SWAT runs with lower resolution DEM.

• 현장농수산연구지
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• 제12권1호
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• pp.39-45
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• 2010

본 연구는 Dianthus spp와 Coreopsis spp의 삽목시기와 용토, Plug 규격이 생육 및 발근 상태에 미치는 영향을 구명하고자 실시하였다. Dianthus spp와 Coreopsis spp 두 품종 모두 삽목 시기가 늦어질 수록 발근율과 발근 수가 낮아졌다. 5월 20일에서 8월20일 삽목 처리구에서는 두 품종 모두 90% 이상 발근하였으나 9월 20일 삽목한 처리구에서는 Dianthus spp와 Coreopsis spp 두 품종이 각각 87%와 82%의 저조한 발근율을 보였고 발근 수 역시 9월 20일 처리구가 가장 저조하였다. 또한 Dianthus spp와 Coreopsis spp 두 품종 모두 모래를 제외한 모든 용토에서 90%이상 발근되었고 peatmoss(1)+perlite(1) 혼합용토에서 발근수가 가장 많았다. Coreopsis spp는 plug 162공에서 발근율이 98%로 가장 높았고 Dianthus spp는 162공과 200공에서 각각 발근율이 95.8%와 96%로 높고 근권부 형성율이 좋았다.

• 한국축산식품학회지
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• 제35권6호
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• pp.793-799
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• 2015

The market sales of premium ice cream have paralleled the growth in consumer desire for rich flavor and taste. Storage temperature is a major consideration in preserving the quality attributes of premium ice cream products for both the manufacturer and retailers during prolonged storage. We investigated the effect of storage temperature (−18℃, −30℃, −50℃, and −70℃) and storage times, up to 52 wk, on the quality attributes of premium ice cream. Quality attributes tested included ice crystal size, air cell size, melting resistance, and color. Ice crystal size increased from 40.3 µm to 100.1 µm after 52 wk of storage at −18℃. When ice cream samples were stored at −50℃ or −70℃, ice crystal size slightly increased from 40.3 µm to 57-58 µm. Initial air cell size increased from 37.1 µm to 87.7 µm after storage at −18℃ for 52 wk. However, for storage temperatures of −50℃ and −70℃, air cell size increased only slightly from 37.1 µm to 46-47 µm. Low storage temperature (−50℃ and −70℃) resulted in better melt resistance and minimized color changes in comparison to high temperature storage (−18℃ and −30℃). In our study, quality changes in premium ice cream were gradually minimized according to decrease in storage temperature up to−50℃. No significant beneficial effect of −70℃ storage was found in quality attributes. In the scope of our experiment, we recommend a storage temperature of −50℃ to preserve the quality attributes of premium ice cream.

• Interaction and multiscale mechanics
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• 제2권1호
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• pp.15-30
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• 2009

The theoretical framework of the interaction fields for multiple scales based on field theory is applied to one-dimensional problem mimicking dislocation substructure sensitive intra-granular inhomogeneity evolution under fatigue of Cu-added steels. Three distinct scale levels corresponding respectively to the orders of (A)dislocation substructures, (B)grain size and (C)grain aggregates are set-up based on FE-RKPM (reproducing kernel particle method) based interpolated strain distribution to obtain the incompatibility term in the interaction field. Comparisons between analytical conditions with and without the interaction, and that among different cell size in the scale A are simulated. The effect of interaction field on the B-scale field evolution is extensively examined. Finer and larger fluctuation is demonstrated to be obtained by taking account of the field interactions. Finer cell size exhibits larger field fluctuation whereas the coarse cell size yields negligible interaction effects.

• Korea-Australia Rheology Journal
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• 제18권4호
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• pp.183-189
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• 2006

High internal phase emulsions are highly concentrated emulsion systems consisting of a large volume of dispersed phase above 0.74. The rheological properties of high internal phase water-in-oil emulsions were measured conducting steady shear, oscillatory shear and creep/recovery experiments. It was found that the yield stress is inversely proportional to the drop size with the exponent of values between 1 and 2. Since the oil phase contains monomeric species, microcellular foams can easily be prepared from high internal phase emulsions. In this study, the microcellular foams combining a couple of thickeners into the conventional formulation of styrene and water system were investigated to understand the effect of viscosity ratio on cell size. Cell size variation on thickener concentration could be explained by a dimensional analysis between the capillary number and the viscosity ratio. Compression properties of foam are important end use properties in many practical applications. Crush strength and Young's modulus of microcellular foams polymerized from high internal phase emulsions were measured and compared from compression tests. Of the foams tested in this study, the foam prepared from the organoclay having reactive group as an oil phase thickener showed outstanding compression properties.