• Asian Pacific Journal of Cancer Prevention
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• v.17 no.2
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• pp.581-590
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• 2016

Resistance to anoikis, a cell-detachment induced apoptosis, is one of the malignant phenotypes which support tumor metastasis. Molecular mechanisms underlying the establishment of this phenotype require further investigation. This study aimed at exploring protein expression profiles associated with anoikis resistance of a metastatic breast cancer cell. Cell survival of suspension cultures of non-metastatic MCF-7 and metastatic MDA-MB-231 cells were compared with their adherent cultures. Trypan blue exclusion assays demonstrated a significantly higher percentage of viable cells in MDA-MB-231 than MCF-7 cell cultures, consistent with analysis of annexin V-7-AAD stained cells indicating that MDA-MB-231 possess anti-apoptotic ability 1.7 fold higher than MCF-7 cells. GeLC-MS/MS analysis of protein lysates of MDA-MB-231 and MCF-7 cells grown under both culture conditions identified 925 proteins which are differentially expressed, 54 of which were expressed only in suspended and adherent MDA-MB-231 but not in MCF-7 cells. These proteins have been implicated in various cellular processes, including DNA replication and repair, transcription, translation, protein modification, cytoskeleton, transport and cell signaling. Analysis based on the STITCH database predicted the interaction of phospholipases, PLC and PLD, and 14-3-3 beta/alpha, YWHAB, with the intrinsic and extrinsic apoptotic signaling network, suggesting putative roles in controlling anti-anoikis ability. MDA-MB-231 cells grown in the presence of inhibitors of phospholipase C, U73122, and phospholipase D, FIPI, demonstrated reduced ability to survive in suspension culture, indicating functional roles of PLC and PLD in the process of anti-anoikis. Our study identified intracellular mediators potentially associated with establishment of anoikis resistance of metastatic cells. These proteins require further clarification as prognostic and therapeutic targets for advanced breast cancer.

• The Korean Journal of Physiology and Pharmacology
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• v.22 no.5
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• pp.513-523
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• 2018

The tumor microenvironment greatly influences cancer cell characteristics, and acidic extracellular pH has been implicated as an essential factor in tumor malignancy and the induction of drug resistance. Here, we examined the characteristics of gastric carcinoma (GC) cells under conditions of extracellular acidity and attempted to identify a means of enhancing treatment efficacy. Acidic conditions caused several changes in GC cells adversely affecting chemotherapeutic treatment. Extracellular acidity did inhibit GC cell growth by inducing cell cycle arrest, but did not induce cell death at pH values down to 6.2, which was consistent with down-regulated cyclin D1 and up-regulated p21 mRNA expression. Additionally, an acidic environment altered the expression of atg5, HSPA1B, collagen XIII, collagen XXAI, slug, snail, and zeb1 genes which are related to regulation of cell resistance to cytotoxicity and malignancy, and as expected, resulted in increased resistance of cells to multiple chemotherapeutic drugs including etoposide, doxorubicin, daunorubicin, cisplatin, oxaliplatin and 5-FU. Interestingly, however, acidic environment dramatically sensitized GC cells to apoptosis induced by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Consistently, the acidity at pH 6.5 increased mRNA levels of DR4 and DR5 genes, and also elevated protein expression of both death receptors as detected by immunoblotting. Gene silencing analysis showed that of these two receptors, the major role in this effect was played by DR5. Therefore, these results suggest that extracellular acidity can sensitize TRAIL-mediated apoptosis at least partially via DR5 in GCs while it confers resistance to various type of chemotherapeutic drugs.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.4
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• pp.327-334
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• 2020

The present study was conducted to assess the relationship between metabolic syndrome, metabolic syndrome score, homeostasis model assessment of insulin resistance (HOMA-IR), and beta-cell function (HOMA-B) in obese Korean adults. The study included 1,860 adults aged 20 years or older from the 2010 Korean National Health and Nutrition Examination Survey (KNHANES) data. Metabolic syndrome and metabolic syndrome score (MSS) were positively associated with HOMA-IR (both P<0.001). HOMA-B levels of elevated blood pressure (P<0.001) and elevated fasting blood glucose group (P<0.001) were significantly lower than the normal group. However, the HOMA-B levels of abdominal obesity (P=0.003) and reduced high-density lipoprotein cholesterol group (P=0.030) were significantly higher than the normal group. Nevertheless, metabolic syndrome (P<0.001) and MSS (P<0.001) were inversely associated with the HOMA-B levels. In conclusion, metabolic syndrome and MSS were positively associated with insulin resistance and inversely associated with beta-cell function in Korean adults with obesity.

• Current Photovoltaic Research
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• v.5 no.4
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• pp.135-139
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• 2017

We fabricated two different transparent conducting oxide thin films of ZnO doped with Ga ($Ga_2O_3$ 0.9 wt%) as well as Al ($Al_2O_3$ 2.1 wt%) (GAZO) and ZnO doped only with Al ($Al_2O_3$ 3 wt%) (AZO). It was investigated how it affects the moisture resistance of the transparent electrode. In addition, $Cu(In,Ga)Se_2$ thin film solar cells with two transparent oxides as front electrodes were fabricated, and the correlation between humidity resistance of transparent electrodes and device performance of solar cells was examined. When both transparent electrodes were exposed to high temperature distilled water, they showed a rapid increase in sheet resistance and a decrease in the fill factor of the solar cell. However, AZO showed a drastic decrease in efficiency at the beginning of exposure, while GAZO showed that the deterioration of efficiency occurred over a long period of time and that the long term moisture resistance of GAZO was better.

• Journal of Sensor Science and Technology
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• v.26 no.1
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• pp.15-23
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• 2017

In this study, bioelectrical impedance analysis, which has been used to assess an alteration in intracellular fluid (ICF) of the body, was applied to detect intravenous infiltration. The experimental results are described as follows. Firstly, when infiltration occurred, the resistance gradually decreased with time and frequency i.e., the resistance decreased with increasing time, proportional to the amount of infiltrated intravenous (IV) solution. At each frequency, the resistance gradually decreased with time, indicating the IV solution (also blood) accumulated in the extracellular fluid (ECF) (including interstitial fluid). Secondly, the resistance ratio started to increase at infiltration, showing the highest value after 1.4 min of infiltration, and gradually decreased thereafter. Thirdly, the impedance ($Z_C$) of cell membrane decreased significantly (especially at 50 kHz) during infiltration and gradually decreased thereafter. Fourthly, Cole-Cole plot indicated that the positions of (R, $X_C$) shifted toward left owing to infiltration, reflecting the IV solution accumulated in the ECF. The resistance ($R_0$) at zero frequency decreased continuously over time, indicating that it is a vital impedance parameter capable of detecting early infiltration during IV infusion. Finally, the mechanism of the current flowing through the ECF, cell membrane, and ICF in the subcutaneous tissues was analyzed as a function of time before and after infiltration, using an equivalent circuit model of the human cell. In conclusion, it was confirmed that the infiltration could be detected early using these impedance parameters during the infusion of IV solution.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.12
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• pp.4995-5000
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• 2014

Background: Cancer constitutes a key pressure on public health regardless of the economy state in different countries. As a kind of highly malignant epithelial tumor, lacrimal gland adenoid cystic carcinoma can occur in any part of the body, such as salivary gland, submandibular gland, trachea, lung, breast, skin and lacrimal gland. Chemotherapy is one of the key treatment techniques, but drug resistance, especially MDR, seriously blunts its effects. As an element of the 60S large ribosomal subunit, the ribosomal protein L39-L gene appears to be documented specifically in the human testis and many human cancer samples of different origins. Materials and Methods: Total RNA of cultured drug-resistant and susceptible lacrimal gland adenoid cystic carcinoma cells was seperated, and real time quantitative RT-PCR were used to reveal transcription differences between amycin resistant and susceptible strains of lacrimal gland adenoid cystic carcinoma cells. Viability assays were used to present the amycin resistance difference in a RPL39-L transfected lacrimal gland adenoid cystic carcinoma cell line as compared to control vector and null-transfected lacrimal gland adenoid cystic carcinoma cell lines. Results: The ribosomal protein L39-L transcription level was 6.5-fold higher in the drug-resistant human lacrimal gland adenoid cystic carcinoma cell line than in the susceptible cell line by quantitative RT-PCR analysis. The ribosomal protein L39-L transfected cells revealed enhanced drug resistance compared to plasmid vector-transfected or null-transfected cells as determined by methyl tritiated thymidine (3H-TdR) incorporation. Conclusions: The ribosomal protein L39-L gene could possibly have influence on the drug resistance mechanism of lacrimal gland adenoid cystic carcinoma cells.

• Korean Journal of Environmental Agriculture
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• v.31 no.1
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• pp.30-36
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• 2012

BACKGROUND: The resistance of rice bakanae disease pathogens against the fungicide prochloraz has been reported. Understanding the resistance mechanisms is an important for better control of the pathogens. In the present study, we investigated the resistance mechanisms of Fusarium fujikuroi CF337 (CF337) against prochloraz. METHODS AND RESULTS: Morphological changes in the cell wall of CF337 grown in potato dextrose broth (PDB) with or without prochloraz was investigated by transmission electron microscopy. Growth inhibition of CF337 was examined in PDB containing prochloraz or an ABC transporter inhibitor or both of them. Cell wall thickness of CF337 grown in PDB with prochloraz was significantly increased from $80.73{\pm}1.99nm$ to $193.11{\pm}7.07nm$. Significant inhibition in the growth of CF337 was observed in the presence of both prochloraz and the inhibitor, but no growth inhibition was observed in the presence of the inhibitor or prochloraz. Sequence analysis of ATP-binding cassette transporter (ABC) gene of CF337 showed 70 to 80% similarities to the genes of the pathogens resistant to other fungicides. CONCLUSION: Efflux transporter system and changes in cell wall thickness were suggested as resistance mechanisms of CF337 against prochloraz.

• Journal of the Korean Institute of Gas
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• v.28 no.1
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• pp.65-72
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• 2024

Green Hydrogen demonstration complex is under conduction in Jeju island which is rich in renewable energy resources and will produces green hydrogen using a water electrolysis systems. In order to check durability of long-term operation, AST(accelerated stress test) was applied and the power pattern based on Jeju Island's wind power was applied. After 800 hours of repeated application of low current and high current, the performance of the PEM water electrolysis cell was reduced by up to 10% and by about 5.5% in operating conditions. As the result of impedance analysis, it can be seen that the electrode polarization resistance greatly increased than ohmic polarization resistance. In addition, when the durability evaluation was conducted by applying the wind power pattern of Jeju Island, the performance of the PEM water electrolysis cell showed up to 1.6% and a decrease of less than 1% in operating conditions. As a result of the impedance, it can be seen that the change of ohmic resistance and electrode polarization resistance is small.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.58 no.4
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• pp.597-601
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• 2009

Green car such as a hybrid electrical vehicle and fuel cell vehicle is developed as a commercial target. UN/ECE/WP29 is developing GTR of HFCV and establishing the regulation and standard of electrical safety by ELSA. The regulation and standard about Electrical safety of vehicle are prescribed in ISO, UN/ECE, FMVSS, Japanese Attachment and so on, in case of insulation resistance is referred to keep more than 100/Vdc, 500/Vac. However, accurate method to measure insulation resistance agreeable to structure of vehicle does not exist now, it is actually that correctness of measurement drops according to the feature of battery and fuel cell stack. In this paper, the method to measure insulation resistance for protection against electrical shock by direct contact or indirect contact in Green Car will be indicated by making a comparison between the insulation measurement in standard of electrical safety and the experiment results for HEV and HFCV.

• Corrosion Science and Technology
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• v.11 no.6
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• pp.247-256
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• 2012

Harmonic current was measured for a dummy cell with various values of resistance, and the procedure developed through the measurements was applied to the investigation of effects of the amplitude of applied frequency and applied potential on the harmonic current of a stainless steel and a carbon steel in chloride containing solutions. From the measurements of harmonic current in the dummy cell, the optimum values of applied frequency and applied potential in harmonic current measurements were found to be 1 mHz and 20 mV (or lower), respectively. Increase in harmonic current with applied frequency was observed in the case where the level of harmonic current is low as in a stainless steel. Decrease in polarization resistance was also noted in this corrosion system with either increasing applied frequency or decreasing applied potential. However, no obvious effects of applied frequency was observed on harmonic current and polarization resistance in a carbon steel in which the level of harmonic current is high.