• The Journal of Pediatrics of Korean Medicine
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• v.23 no.3
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• pp.9-36
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• 2009

Objectives The purpose of this study is to examine the effect of a concurrent administration of JUJSTK and AJ on atopic dermatitis in an in-vivo experiment. Thus, this study is expressed by using NC/Nga atopic dermatitis mice which have histological and clinical similarities to that of humans have been used. Methods Clinical skin score, hematology, serum total IgE and IgG1 of the mouse was evaluated, and cytokine levels, total number of the cells, immunohistochemical staining, histological features of axillary lymph node(ALN), peripheral blood mononuclear cells(PBMCs), and a dorsal skin tissue of the mouse were analyzed. Results Oral administration of JUJSTK and concurrent administration of JUJSTK and AJ lowered the clinical skin score, total cell number of WBC, eosinophils in blood, and serum total of IgE & IgG1, IFN-$\gamma$, IL-5, IL-13, IL-17. In addition, total cell number of ALN and dorsal skin tissue, absolute cell number of $CD3e^+$ T cell, $CD4^+$ Th cell, $CD8^+$ c/sT cell, $CD3^+CCR3^+$ cell, $CCR3^+$ cell, $CD3^+CD69^+$, $CD4^+CXCR5^+$ in ALN, PBMCs, absolute cell number of $CCR3^+$, $CD3^+/CD69^+$, $CD11b^+/Gr-1^+$, $CD11b^+/Gr-1^+$ in dorsal skin tissue, Eotaxin2 mRNA, CCR3 mRNA in dorsal skin tissue and gene expression of IL-5 mRNA, IL-13 mRNA in ALN were significantly decreased. Furthermore, thickness of epidermis infiltrated inflammatory immune cell & mast cell in dermis, histological infiltration of mast cell, the size of inflammatory lymphocytes cells & plasma cells in ALN and histological infiltration of $CD4^+$ & $CCR3^+$ in ALN and dorsal skin tissue were significantly decreased as well. Conclusions Concurrent administration of JUJSTK and AJ on atopic dermatitis in an in-vivoexperiment by using an NC/Nga atopic dermatitis mouse was very effective as an atopic dermatitis treatment.

• Journal of Acupuncture Research
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• v.17 no.1
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• pp.189-219
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• 2000

To study the effects of anti-cancer, anti-metastasis and immune response improvement effects of aqua-acupuncture with Rubi Fructus infusion solution, we used Rubi Fructus infusion solution(taken by water-alcohol method) put into Chung-wan (CV12) and Chok-Samni(ST36) of BALB/c or C57BL/6 which are corresponding to humanbody. We observed the cytotoxicity, the effect on the expression of MMP-9 gene, the ability to control cancer cell proliferation, change of body weight, surviving number, median surviving time, increase of life span, changes in amount of leukocyte, erythrocyte, platelet, total protein, creatinine, glucose and LDH, weight of spleen, number of pulmonary colony, histological analysis on tissue metastasis of lung and liver, splenic cell proliferation, the expression of cytokine gene, the number of $CD4^+$, $CD8^+$, $CD19^+$ and NK cell, and concluded like this. The results were obtained as follows : 1. Effects of Anti-cancer 1) The cytotoxicity about B16-F10 cell line of $2^0$, $2^{-1}$, $2^{-2}$, $2^{-3}$, $2^{-5}$, $2^{-6}$, $2^{-7}$, $2^{-8}$ diluent groups in Rubi Fructus infusion solution treatment was inhibited significantly, compared with control group. 2) The cytotoxicity about HT1080 cell line of $2^0{\sim}2^{-8}$ diluent groups in Rubi Fructus infusion solution treatment was inhibited significantly, compared with control group. 3) The effect on expression of MMP-9 gene was inhibited significantly in all the sample groups, compared with control group. 4) The effect on the control-ability on the cancer cell proliferation showed cytotoxicity significantly in $2^0$, $2^{-1}$, $2^{-2}$, $2^{-3}$, $2^{-4}$, $2^{-5}$, $2^{-6}$, $2^{-7}$, diluent groups. 2. Effects of Anti-metastasis 1) S-180 cancer cell line transplants in BALB/c mice were inhibited significantly in weight increase in all the sample groups, compared with control group. The surviving number increased in almost sample groups, except one group put into Chok-Samni(ST36) with 20% Rubi Fructus infusion solution treatment group that showed same number of the control group. 2) S-180 cancer cell line transplants in BALB/c mice showed high MST significantly in almost sample groups, compared with control group. But one group put into Chok-Samni(ST36) with 20% Rubi Fructus infusion solution showed low MST than control group. 3) The group injected in vein with B16-F10 cancer cell line in C57BL/6 mice showed increased ILS than control group significantly in anti-metastasis test. 3. Effects of Immune response improvement 1) The group injected in vein with B16-F10 cancer cell line in C57BL/6 mice were increased significantly in the number of leukocyte and glucose, and decreased significantly in the amount of platelet and LDH, compared with control group. However, there's no significant increase or decrease in number of erythrocyte, total protein and creatinine. 2) We couldn't find any significant relation in spleen weight of the sample group. 3) In pulmonary colony, sample group was decreased significantly, compared with control group. 4) Histological analysis of sample group inhivited compared with that of control group in both of lung and liver. 5) In immune system, all the sample groups showed having more relevancy to the effect on splenic cell proliferation than normal group. 6) Cytokine gene increased in almost sample groups, except one group treated with $50{\mu}g/m{\ell}$ Rubi Fructus infusion solution on IL-12. 7) In flow cytometry there's no significant relation in number of $CD8^+$ cell, however, the number of $CD4^+$, $CD19^+$ cell and NK cell in sample group had more relation than in control group. Above the results showed that aqua-acupuncture of Rubi Fructus solution has effects of anti-cancer, and-metastasis and immune response improvement.

• Journal of Acupuncture Research
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• v.17 no.1
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• pp.251-286
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• 2000

To study the effects of anti-cancer, anti-metastasis and immune response improvement of aqua-acupuncture with Cistanches Herba infusion solution, we used Cistanches Herba infusion solution(taken by water-alcohol method) put into Chung-wan(CV12) and Chok-Samni(S36) of BALB/c or C57BL/6 which are corresponding to human body. We observed the cytotoxicity, the effect on the expression of MMP-9 gene, the ability to control cancer cell proliferation, change of body weight, surviving number, MST, ILS, changes in amount of WBC, RBC, PLT, total protein, creatinine, glucose and LDH, weight of spleen, number of pulmonary colony, histological analysis on tissue metastasis of lung and liver, splenic cell proliferation, the expression of cytokine gene, the number of $CD4^+$, $CD8^+$, $CD19^+$ and NK cell, and concluded like this. The results were obtained as follows : 1. The cytotoxicity about B16-F10 cell line of $2^0$, $2^{-1}$, $2^{-2}$, $2^{-5}$ diluent groups in Cistanches Herba infusion solution treatment was inhibited significantly, compared with control group. 2. The cytotoxicity about HT1080 cell line of $2^0$, $2^{-1}$, $2^{-2}$, $2^{-3}$, $2^{-5}$, $2^{-8}$ diluent groups in Cistanches Herba infusion solution treatment was inhibited significantly, compared with control group. 3. The effect on expression of MMP-9 gene was decreased in all the sample groups, compared with control group. 4. The effect on the control-ability on the cancer cell proliferation showed cytotooicity significantly in $2^0$, $2^{-1}$, $2^{-2}$, $2^{-3}$, $2^{-4}$, $2^{-5}$ diluent groups. 5. S-180 cancer cell line transplants in BALB/c mice were inhibited significantly in weight increase in all the sample groups, compared with control group. The surviving number increased in almost sample groups, except one group put into Chok-Samni(S36) with 20% Cistanches Herba infusion solution treatment group that showed same number of the control group. 6. S-180 cancer cell line transplants in BALB/c mice showed high MST and ILS significantly in almost sample groups, compared with control group. But one group put into Chok-Samni(S36) with 20% Cistanches Herba infusion solution treatment group showed low MST and ILS than control group. 7. The sample group injected in vein with B16-F10 cancer cell line in C57BL/6 mice showed increased ILS compared with control group significantly in anti-metastasis test. 8. The sample group injected in vein with B16-F10 cancer cell line in C57BL/6 mice were increased significantly in the number of WBC and glucose, and decreased significantly in the amount of LDH, compared with control group. However, there's no significant increase or decrease in number of RBC, PLT, total protein and creatinine. 9. We couldn't find any significant relation in spleen weight of the sample group. 10. In pulmonary colony, sample group was decreased significantly, compared with control group. 11. Histological analysis of sample group inhivited compared with that of control group in both of lung and liver. 12. In immune system, all the sample groups showed having more relevancy to the effect on splenic cell proliferation than normal group. 13. The effect on cytokine gene expression of all the sample groups were increased than control group. 14. In flow cytometry there's no significant relation in number of $CD8^+$, $CD19^+$ cell, however, the number of $CD4^+$ cell and NK cell in sample groups were increased than in control group. Above the results showed that aqua-acupuncture of Cistanches Herba infusion solution has effects of anti-cancer, anti-metastasis and immune response improvement.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 1996.04a
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• pp.121-124
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• 1996

In general, cellular manufacturing system can be constructed by the following two steps. The first step forms machine cells and part families, and the second step determines cell layout based on the result of first step. Cell layout has to be considered when cell is formed becauese the result of cell formation affects it. This paper presents a cell formation algorithm and proposes an integrated mathematical model for cell formation and cell layout. The cell formation algorithm minimizes the number of exceptional element in cellular manufacturing system. New concept for similarity and incapability is introduced, based on machine-operation incidence matrix and part-operation incidence matrix. One is similarity between the machines, the other is similarity between preliminary machine cells and machines. The incapability identifies relations between machine cells and parts. In this procedure, only parts without an exceptional element are assigned to machine cell. Bottleneck parts are considered with cell layout design in an integrated mathematical model. The integrated mathematical model determines cell layout and assigns bottleneck parts to minimize the number of exceptional element and intercell moving distance, based on linearixed 0-1 integer programming. The proposed algorithm is illustrated by using numerical examples.

• Journal of Korean Institute of Industrial Engineers
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• v.19 no.3
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• pp.91-102
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• 1993

This paper suggests an approach to quantitative evaluation of a manufacturing flexibility in automated manufacturing systems. The flexibility of a cell is newly defined and evaluated in use of the environmental change factors which may influence flexibility for satisfying a manufacturing performance objective. The number of machines, the number of operations, machine breakdowns and processing times are considered for this cell flexibility measure. The cell flexibility measures the extent that the cell utilizes the processes to acquire high throughput. Simulation program written in SLAM System was used to help measure cell flexibility. The proposed cell flexibility measure provides a prediction of the influence of the factors on throughput performance, and applies in case of comparison of existing system and a new system, changes in operation conditions of a cell, and comparison of rival machines. Therefore it can be used as decision making criteria for system justification.

• Journal of Korean Institute of Industrial Engineers
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• v.20 no.2
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• pp.51-64
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• 1994

Cellular manufacturing requires formation of machine cells that can produce families of parts with similar processing requirement. The purpose of cell formation is to create separable machine clusters and part families simultaneously. However, the cell formation process often includes the identification of exceptional elements. This paper presents cell formation method under consideration of alternative routings in FMS which consists of machines capable of multi-processing and parts which require more than one operation. We suggest theorems to calculate the maximum number of machine cell and part family which have no exceptional elements. We also develop a cell formation algorithm which is based on the suggested theorem. A numerical example is provided to illustrate the proposed theorem and algorithm.

• Reproductive and Developmental Biology
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• v.28 no.1
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• pp.29-36
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• 2004

The objective of this study was to investigate the effects of amino acid supplementation of oocyte maturation medium on 1st polar body(PB) extrusion, embryo development and blastocsyt cell number. In experiment 1, Cumulus oocyte complexes(COCs) were matured in in vitro maturation(IVM) medium supplemented with 1, 2, or 4-fold of 10 $\mu$l/ml MEM non-essential amino acid(NEAA) and 20 Park, $\mu$ l/ml BME essential amino acid(EAA). The PB extrusion rate of oocytes matured in 1-fold amino acid group was significantly higher than that matured in medium without amino acid (p＜0.05), but it was decreased by the increase of the dosage of amino acid. There were no difference in the percentage of embryos reaching 2-cell, 8-cell and blastocyst in all treatments. The number of trophectoderm(TE) cells and total cell number of blastocysts were highest in 2-fold amino acid group, and the number of inner cell mass(ICM) cells was increased by the increase of the dosage of amino acid. In experiment 2, COCs were matured in IVM medium with 1, 5, or 10 mg/ml lactalbumin hydrolysate(LAH). The PB extrusion rate of oocytes matured in medium with 5 mg LAH was significantly higher than that matured in medium with 1 mg LAH (p＜0.05). The development rate to the blastocyst stage was significantly higher in non-supplement and 1 mg LAH group than in 5 mg and 10 mg LAH group (p＜0.05). The number of TE cells and total cell number did not differ among treatment groups, but the number of ICM cells was increased by the increase of LAH supplement. These results suggested that the supplement of certain group of amino acid in IVM medium effective on the quality of blastocyst, and further studies will be accompany with the search of new sources of amino acid used for the use of in vitro embryo production.

• Journal of international Conference on Electrical Machines and Systems
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• v.3 no.2
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• pp.200-206
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• 2014

This paper describes magnetically levitated vehicle with hybrid magnets, which have been studied by the authors in place of streetcars or conveyance system. An experimental vehicle of 20kg was magnetically levitated by using a small number of dry-cell batteries, which consisted of 10 Ni-MH cells of 1900mAh in series. The magnets were activated sequentially, because the internal resistance of the batteries suppressed the maximum current. The vehicle was kept levitating for about 2 hours and was stable against disturbance due to instantaneous external force. In this paper, dynamic characteristics of the magnetically levitated vehicle using a small number of dry cell batteries are presented.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.68-71
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• 2004

Recently, a great deal of research and development of a fuel cell have been carried out to solve problems on the drain of fossil fuel, air pollution and global warning. In order to improve the efficiency of a fuel cell, it is necessary to clarify the flow in separator. In this study, distributions of velocity flow rate and pressure, and streamlines are examined in detail from numerical analysis with CFD code. In the experiment the distribution of flow rate is measured and flow in the each grooves of the separator is visualized by dye method changing Reynolds number. Furthermore, effects of size of the inlet and outlet manifolds and shape of ribs near the inlet outlet on the distributions of flow and pressure are examined.

• Transactions of the Korean hydrogen and new energy society
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• v.26 no.2
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• pp.127-135
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• 2015

High-Temperautre Polymer Electrolyte Membrane Fuel Cell (HT-PEMFC) with phosphoric acid-doped polybenzimidazole (PBI) membrane has high power density because of high operating temperature from 100 to $200^{\circ}C$. In fuel cell stack, heat is generated by electrochemical reaction and high operating temperature makes a lot of heat. This heat is caouse of durability and performance decrease about stack. For these reasons, heat management is important in HT-PEMFC. So, we developed HT-PEMFC model and study heat flow in HT-PEMFC stack. In this study, we placed coolant plate number per cell number ratio as variable and analysed heat flow distribution in stack.