• Biotechnology and Bioprocess Engineering:BBE
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• 제8권1호
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• pp.32-36
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• 2003

Production of camptothecin ((PT) from callus cultures of Camptotheca acuminata Decne was affected by light and culture conditions. Among the culture media tested, modified B5 medium containing 3% (w/v) sucrose, 2 mg/L B,4-D, 2 times of MS medium vitamins, 500 mg/L casein hydrolysate, 250 mg/L myo-inositol, 0.05% (w/v) activated charcoal, and 0.15% (w/v) gelite was used for callus induction . The highest cell growth and CPT production were obtained in dark and green light condition, respectively. Photoperiod has no effect on cell growth and CPT production. Both cell growth and CPT production were also influenced by combination ratio of red and blue light .Cell growth and CPT production were the highest in the ratio of red and blue light,90:10.

• 한국미생물·생명공학회지
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• 제28권5호
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• pp.285-290
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• 2000

The effects of dissolved oxy-gen(DO) concentration and DO control modes on cell growth in a 4L computer-controlled bioreactor system were investigated using Phellinus linteus WI-001, a producer of protein-polysaccharides having potent anticancer activi-ties. When DO was controlled at about 20%, maximum cell concentration and specific growth rate of the strain were observed to increase 36% and 64%, respectively, as compared to the experiment performed without DO control. By adopting cascade automatic control of DO ar 20% in a mixed automatic control mode using computer-con-trolled program, 19.5g/L of maximum cell concentration was obtained. These results showed that the mixed auto-matic control mode was the effective method for enhancing cell growth of the shear sensitive Phellinus linteus.

• 한국가축번식학회지
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• 제22권1호
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• pp.101-104
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• 1998

This study examined the effects of growth factors in TCM199 on bovine 1-cell embryos development in vitro. After 6 day to 11 day in culture, 15.8%(19/120), 15.3%(20/130), 21.8%(35/160), 27.0%(56/207), 26.3%(53/201) and 30.7%(40/130) of the 1-cell embryos developed into expanding blastocysts in su, pp.ementing TCM199 with control, insulin, IGF-I, IGF-II, FGF and EGF, respectively. Hatching rate of 1-cell embryos in su, pp.ementing TCM199 with FGF, EGF and IGF-II were 21.4%(53/247), 20.3%(42/206) and 16.8%(41/243), respectively. The beneficial effect of growth factors on embryo development in vitro could be duplicated. These data indicate that the presence of FGF, EGF or IGF-II in the culture medium is beneficial for embryo development in vitro and accelerate cell differentiation.

• 약학회지
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• 제41권1호
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• pp.94-98
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• 1997

Ceramide. a product of sphingomyelin hydrolysis, has been proposed as a lipid second messenger mediating antiproliferative activation. In this study, we examined the role of the cell cycle-related proteins in the ceramide-mediated growth suppression. Treatment of U-937 cells with C$_2$-ceramide(N-acetylsphingosine) resulted in growth suppression in a time- and concentration dependent manner. Ceramide induced concentration dependent dephosphorylation of retinoblastoma gene product (Rb). Rb remains hypophosphorylated in synchronized cells even after serum stimulation in the presence of ceramide. Ceramide decreased the expression of cyclin D$_1$ and cyclin E levels. These results suggest that antiproliferative effect of ceramide is associated with hypophosphorylation of Rb and decreased expression of cyclin D1 and cyclin E.

• 미생물학회지
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• 제25권3호
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• pp.189-197
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• 1987

배지성분으로 첨가된 glutamate의 농도가 균의성장고 tylosin 생합성에 미치는 영향을 조사하였다. 그 결과 oxaloacetate를 공동기질로 사용하는 효소의 활성에 의하여 균의 성장과 tylosin의 생합성이 조절됨을 알았다. 즉, citrate synthase와 aspartate aminotransferase의 활성은 균의 성장에 아주 긴요하며 methylmalonyl-CoA carboxytransferase의 활성은 tylosin 생합성에 아주 중요한 효소임을 알았다. Glutamate의 농도는 우의 효소의 활성에 직접적으로 영향을 주고 있음을 알았다.

• KSBB Journal
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• 제13권4호
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• pp.431-437
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• 1998

Optimal media and cultural conditions for the production of prodigiosin-like pigment were established using Serratia sp. KH-95. Glucose and phosphate(K2PO4) stimulated the cell growth, but inhibited the production of pigment at concentration levels of above 10 g/L and 2.0 g/L, respectively. Addition of soy been oil or rice oil to the production medium accelerated cell growth up to more than 2-3 times, but the production of prodigiosin increased about 15-20% in spite of the good cell growth. The effect of pH on the production of pigment was investigated in a 5 liter-bioreactor. When the pH of culture broth was maintained below 8.0, most of pigment was attached to the surface of cells. When the pH of culture broth was above 8.5, however, about 70% of total pigment was suspended in the supernatant of the broth. The cell growth and production of pigment were inhibited at dissolved oxygen concentration of below 10% of air-saturation.

• KSBB Journal
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• 제14권5호
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• pp.534-538
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• 1999

식물세포배양을 위한 bioreactor의 운전조건 최적화를 위해 Nicotiana tabacum 현탁세포를 model system으로 bioreator의 종류, 교반기의 형태, 그리고 통기량에 따른 세포생장을 관찰하였다. Bioreactor로는 stirred tank bioreactor과 airlift bioreactor를 사용하였으며 두가지 배양기 모두 flask에서의 생장보다 낮은 생장을 보였으며 stirred tank bioreactor보다는 airlift bioreator에서 높은 세포농도를 얻을 수 있었다. 교반기의 종류에 따른 세포의 생장은 큰 차이가 없었으나 hollowed paddle impeller를 사용하였을 경우에는 배양기간 동안 세포의 크기가 작게 유지되었다. 통기량을 0.30 vvm으로 유지하는 경우에 가장 좋은 세포생장을 관찰할 수 있었으며 1.0 vvm이상의 통기량에서는 과도한 foam의 형성과 세포의 갈색화 현상을 보이며 세포의 생장도 저해되었다. 또한 통기량이 증가할수록 세포크기지수가 감소하는 결과를 보였다.

• Applied Biological Chemistry
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• 제39권5호
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• pp.343-348
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• 1996

Mouse의 대장에서 분리된 E. coli LI-10 균주를 보조균주로 하여 Cellulomonas sp. KL-6과 혼합배양한 결과, 균체증식은 주균주와 보조균주를 1 : 1(v/v)의 비로 혼합하였을 때 가장 좋았다. 혼합배양은 주균주 단독배양 보다 균체증식을 63% 정도로 증가시켰으며 두 균주의 분포도는 10 : 1 비율로 KL-6 균주가 주로 분포되어 있었다. 0.1%의 $CaCO_3$의 첨가는 무첨가에 비해 각 배양기간별로 pH를 상승시켜 어느정도의 균체증식을 가져왔다. Filter paper 배지에서 혼합배양시, 본 균주들은 cellobiose를 월등하게 많이 생산하였으나 glucose는 검출되지 않았다. 균체증식 최적배지에서 4일간 혼합배양하였을 때, $1.0\;g/{\ell}$의 균체량을 생산하여 기본배지인 CMC 배지에서 생산한 균체량보다 53% 정도가 증가되었다.

• 대한치과보철학회지
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• 제42권2호
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• pp.175-191
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• 2004

Statement of problem: Platelet-rich plasma(PRP) is well known to be very effective method to stimulate and accelerate the healing of bone and soft tissue. However, there are few reports which deal with the mechanisms of the PRP on the activation of the osteoblasts. Purpose: This study was aimed to investigate the effect of growth factors in PRP on the activity of osteoblasts. Material and method: To evaluate the effect on human, human osteoblast cell line was cultured. PRP was extracted from the blood of a healthy volunteer. Using the recombinant growth factors of PDGF, $TGFT-\beta$, IGF-1, bFGF which are mainly found at bone matrix and their neutralizing antibody, the effect of PRP on the attachment and proliferation of osteoblasts was evaluated. To evaluate the autocrine and paracrine effects, conditioned media(CM) of PRP was made and compared with PRP. By the western blot analysis, the expression of growth factors in PRP, CM was examined. Cell morphology was compared by the light microscope. Results : 1) The effects of CM on osteoblast were similar to the effects of PRP. 2) PRP, CM, recombinant $TGF-\beta$, bFGF, IGF-1 showed significantly higher cellular attachment than control(p<0.05) in the cell attachment assay. In the cell proliferation assay, PRP, CM, recombinant $TGF-\beta$, IGF-1, bFGF, PDGF increased significantly cell proliferation(p<0.01). Among the recombinant growth factors, IGF-1 showed the highest cellular attachment and proliferation. 3) In the western blot assay, bFGF, IGF-1, PDGF weve equally expressed in PRP and CM. 4) The attachment of osteoblast cell decreased significantly after the addition of neutralizing antibody against $TGF-\beta$, IGF-1(p<0.05). In the cell proliferation assay, the addition of neutralizing antibody against $TGF-\beta$, bFGF, PDGF, IGF-1 decreased significantly the cellular proliferation(p<0.05). The amount of decreasing in the cell attachment and proliferation is the highest in at-lGF-1. 5) The cells in control group were flattened and elongated with a few cellular processes in the a light microscope. But, the cells appeared as spherical, plump cells with well developed cellular processes in experimental groups. The cells in PRP and CM had more prominent developed features than recombinant growth factor groups. Conclusions : These findings imply that PRP maximize the cellular activity in early healing period using the synergistic effect, autocrine, paracrine effects of growth factors and increase the rate and degree of bone formation.

• 한국콘텐츠학회논문지
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• 제9권11호
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• pp.247-253
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• 2009

본 연구는 국소온열요법(Local Hyperthermia)에 사용되는 발열체(Thermo-rod)의 온도에 따른 세포성장률의 변화를 관찰하고자 한다. 연구에 사용된 발열체는 듀플렉스 스테인리스 강(Duplex Stainless Steel)을 이용하여 개발되었다. 세포성장률을 관찰하기 위하여 CPAE세포와 Osteoblast세포를 이용하였다. 각각의 세포를 well에 분주 후 3일, 6일, 9일, 12일, 15일 동안 배양만 한 군을 대조군으로 하고 well에 발열체를 식립 후 세포를 분주하여 3일 간격(3일, 6일, 9일, 12일, 15일)으로 하루 30분 유도가열을 실시하여 15일 간 배양한 군을 시험군으로 하였다. CPAE세포와 Osteoblast세포의 성장률을 관찰한 결과 두세포 모두 3일의 대조군과 시험군 모두 세포 성장률이 급격히 상승하다 6일의 대조군과 시험군 모두 급격히 감소하고 9일과 12일 그리고 15일의 대조군과 시험군의 성장률은 불규칙하게 감소하였다. 이러한 성장률 관찰 결과 두종의 세포 모두 약 $41^{\circ}C$의 온도를 가한 시험군과 온도를 가하지 않은 대조군의 차이가 없다. 따라서 CPAE세포와 Osteoblast세포는 발열체에 의한 온도(약 $41^{\circ}C$)에 영향이 없는 것으로 판단된다.